##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AspEI AspI AviII
BalI BbeI BbrPI BbvCI BcgI
BglI BsaAI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII Bst1107I BstEII BstPI
BstUI BstXI BstZ17I CfoI Cfr10I
ClaI CpoI DrdI Eam1105I Eco47III
EcoNI EcoO65I FseI FspI HaeII
HhaI HinP1I HpaI KasI KspI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NgoMIV
NotI NruI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 4585 GGTACC G^GTACC
AcyI 1007 GACGCC G[GA]^CG[CT]C
AocI 1400 CCTGAGG CC^T[ACGT]AGG
Asp718I 4585 GGTACC G^GTACC
AvrII 3586 CCTAGG C^CTAGG
BamHI 576 GGATCC G^GATCC
BanI 4585 GGTACC G^G[CT][GA]CC
BlnI 3586 CCTAGG C^CTAGG
BlpI 3179 GCTCAGC GC^T[ACGT]AGC
BpmI 2017 CTGGAG CTGGAG(16/14)
Bpu1102I 3179 GCTCAGC GC^T[ACGT]AGC
BsaBI 3571 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1007 GACGCC G[GA]^CG[CT]C
BsaI 3645 GAGACC GGTCTC(1/5)
BsiEI 155 CGGCCG CG[GA][CT]^CG
BsmBI 3290 CGTCTC CGTCTC(1/5)
BssSI 1082 CTCGTG CACGAG(-5/-1)
BstBI 4647 TTCGAA TT^CGAA
Bsu36I 1400 CCTGAGG CC^T[ACGT]AGG
CelII 3179 GCTCAGC GC^T[ACGT]AGC
EaeI 155 CGGCCG [CT]^GGCC[GA]
EagI 155 CGGCCG C^GGCCG
EclXI 155 CGGCCG C^GGCCG
Eco52I 155 CGGCCG C^GGCCG
Eco81I 1400 CCTGAGG CC^T[ACGT]AGG
Hin1I 1007 GACGCC G[GA]^CG[CT]C
KpnI 4585 GGTACC GGTAC^C
MaeII 1064 ACGT A^CGT
MamI 3571 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 3803 CAGCTG C[AC]G^C[GT]G
NdeI 5369 CATATG CA^TATG
NheI 651 GCTAGC G^CTAGC
NspV 4647 TTCGAA TT^CGAA
PflMI 1775 CCACAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 53 GGGACCT [GA]G^G[AT]CC[CT]
PstI 5315 CTGCAG CTGCA^G
PvuII 3803 CAGCTG CAG^CTG
SexAI 1597 ACCTGGT A^CC[AT]GGT
SfuI 4647 TTCGAA TT^CGAA
StuI 891 AGGCCT AGG^CCT
Van91I 1775 CCACAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 843 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwNI 2063 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
5606 CAGCCACTG
AspHI 1591 GAGCTC G[AT]GC[AT]^C
3164 GAGCTC
AvaI 170 CCCGGG C^[CT]CG[GA]G
2263 CCCGGG
BciVI 3999 GGATAC GTATCC(6/5)
4463 GTATCC
BclI 4870 TGATCA T^GATCA
4880 TGATCA
BglII 2624 AGATCT A^GATCT
4415 AGATCT
BsaWI 3128 ACCGGA [AT]^CCGG[AT]
4119 TCCGGT
BseRI 4449 CTCCTC GAGGAG(10/8)
1208 GAGGAG
BsgI 3806 CTGCAC GTGCAG(16/14)
1912 GTGCAG
BsiHKAI 1591 GAGCTC G[AT]GC[AT]^C
3164 GAGCTC
BsoBI 170 CCCGGG C^[CT]CG[GA]G
2263 CCCGGG
BspHI 500 TCATGA T^CATGA
4756 TCATGA
BspMI 297 ACCTGC ACCTGC(4/8)
1332 GCAGGT
DraIII 1596 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
5305 CACTTGGTG
EcoRV 4105 GATATC GAT^ATC
5113 GATATC
FauI 177 CCCGC CCCGC(4/6)
2388 CCCGC
FbaI 4870 TGATCA T^GATCA
4880 TGATCA
HgaI 1007 GACGC GACGC(5/10)
3289 GCGTC
HincII 220 GTTGAC GT[CT]^[GA]AC
988 GTTGAC
HindII 220 GTTGAC GT[CT]^[GA]AC
988 GTTGAC
NcoI 44 CCATGG C^CATGG
570 CCATGG
PacI 3914 TTAATTAA TTAAT^TAA
4276 TTAATTAA
RcaI 500 TCATGA T^CATGA
4756 TCATGA
SacI 1591 GAGCTC GAGCT^C
3164 GAGCTC
SapI 724 GAAGAGC GCTCTTC(1/4)
671 GCTCTTC
SfcI 741 CTACAG C^T[GA][CT]AG
5315 CTGCAG
SmaI 170 CCCGGG CCC^GGG
2263 CCCGGG
XcmI 1771 CCATCCACAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2407 CCAAGAATTATTTGG
XmaCI 170 CCCGGG C^CCGGG
2263 CCCGGG
XmaI 170 CCCGGG C^CCGGG
2263 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApaI ApoI AseI AsnI
Asp700I AvaII BanII BbsI BbvI
BcnI BfaI BfrI BmyI BpuAI
BsaJI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PleI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI