##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccIII AclI AflII AgeI Aor51HI
AscI AseI AsnI Asp700I AspI
AviII BciVI BclI BfrI BsaBI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrGI Bst1107I BstZ17I
ClaI CpoI DraI DrdI Eco47III
EcoRI EcoRV EcoT22I FbaI FspI
HindIII HpaI MamI MfeI MluI
MroI MunI NheI NotI NruI
NsiI PacI PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI ScaI SfiI SgrAI
SnaBI SpeI SspBI SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 1991 GACGTC GACGT^C
Acc65I 88 GGTACC G^GTACC
AcsI 2623 AAATTC [GA]^AATT[CT]
AhdI 5036 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2623 AAATTC [GA]^AATT[CT]
Asp718I 88 GGTACC G^GTACC
AspEI 5036 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4153 CCTAGG C^CTAGG
BamHI 1855 GGATCC G^GATCC
BbeI 860 GGCGCC GGCGC^C
BcgI 314 CGAGGTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 4153 CCTAGG C^CTAGG
BlpI 3836 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3836 GCTTAGC GC^T[ACGT]AGC
BsaWI 2029 ACCGGA [AT]^CCGG[AT]
BsiEI 2054 CGGCCG CG[GA][CT]^CG
BspLU11I 4617 ACATGT A^CATGT
BssHII 3398 GCGCGC G^CGCGC
BssSI 949 CACGAG CACGAG(-5/-1)
BstBI 399 TTCGAA TT^CGAA
BstEII 1292 GGTCACC G^GT[ACGT]ACC
BstPI 1292 GGTCACC G^GT[ACGT]ACC
CelII 3836 GCTTAGC GC^T[ACGT]AGC
EagI 2054 CGGCCG C^GGCCG
Eam1105I 5036 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 3099 GAAGAG CTCTTC(1/4)
EclXI 2054 CGGCCG C^GGCCG
Eco52I 2054 CGGCCG C^GGCCG
EcoO65I 1292 GGTCACC G^GT[ACGT]ACC
FseI 588 GGCCGGCC GGCCGG^CC
HincII 4235 GTTGAC GT[CT]^[GA]AC
HindII 4235 GTTGAC GT[CT]^[GA]AC
KasI 860 GGCGCC G^GCGCC
KpnI 88 GGTACC GGTAC^C
Ksp632I 3099 GAAGAG CTCTTC(1/4)
KspI 2046 CCGCGG CCGC^GG
MslI 4819 CATTCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 589 GCCGGC GCC^GGC
NarI 860 GGCGCC GG^CGCC
NgoMIV 589 GCCGGC G^CCGGC
NspV 399 TTCGAA TT^CGAA
PaeR7I 3421 CTCGAG C^TCGAG
SacII 2046 CCGCGG CCGC^GG
SfoI 860 GGCGCC GGC^GCC
SfuI 399 TTCGAA TT^CGAA
SmaI 1708 CCCGGG CCC^GGG
SphI 788 GCATGC GCATG^C
SspI 4670 AATATT AAT^ATT
XhoI 3421 CTCGAG C^TCGAG
XmaCI 1708 CCCGGG C^CCGGG
XmaI 1708 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 285 GTCTAC GT^[AC][GT]AC
2456 GTAGAC
AflIII 4389 ACGTGT A^C[GA][CT]GT
4617 ACATGT
Alw44I 1272 GTGCAC G^TGCAC
2214 GTGCAC
ApaLI 1272 GTGCAC G^TGCAC
2214 GTGCAC
AvaI 1708 CCCGGG C^[CT]CG[GA]G
3421 CTCGAG
BbrPI 2100 CACGTG CAC^GTG
4388 CACGTG
BglII 246 AGATCT A^GATCT
1050 AGATCT
BsmBI 3359 CGTCTC CGTCTC(1/5)
79 GAGACG
BsoBI 1708 CCCGGG C^[CT]CG[GA]G
3421 CTCGAG
DraIII 1810 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4726 CACCCAGTG
HgaI 81 GACGC GACGC(5/10)
4961 GCGTC
NdeI 5138 CATATG CA^TATG
5157 CATATG
PmaCI 2100 CACGTG CAC^GTG
4388 CACGTG
PmlI 2100 CACGTG CAC^GTG
4388 CACGTG
PshAI 311 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1987 GACTGACGTC
SacI 2308 GAGCTC GAGCT^C
3050 GAGCTC
SexAI 421 ACCTGGT A^CC[AT]GGT
4104 ACCTGGT
StuI 1308 AGGCCT AGG^CCT
3687 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SmlI Sse8387I
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII