##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AspEI AviII
AvrII BanI BbeI BclI BlnI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FbaI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MluI MroI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 2511 GTAGAC GT^[AC][GT]AC
ApaI 3835 GGGCCC GGGCC^C
AspI 3329 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 4 GGATCC G^GATCC
BbrPI 2809 CACGTG CAC^GTG
BcgI 3410 GCAGTCCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2542 GTATCC GTATCC(6/5)
BglI 4406 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1296 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1296 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 3956 TGTACA T^GTACA
BspLU11I 2586 ACATGT A^CATGT
BsrBI 1545 GAGCGG CCGCTC(-3/-3)
BsrFI 784 ACCGGC [GA]^CCGG[CT]
BsrGI 3956 TGTACA T^GTACA
CelII 1296 GCTGAGC GC^T[ACGT]AGC
Cfr10I 784 ACCGGC [GA]^CCGG[CT]
DraIII 501 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2514 GACCCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 4380 AGCGCC [GA]GCGC^[CT]
PflFI 3329 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2809 CACGTG CAC^GTG
PmlI 2809 CACGTG CAC^GTG
PpuMI 1447 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 3538 GAGCTC GAGCT^C
SapI 126 GAAGAGC GCTCTTC(1/4)
ScaI 3572 AGTACT AGT^ACT
SexAI 65 ACCAGGT A^CC[AT]GGT
SfiI 4405 GGCCTGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 3956 TGTACA T^GTACA
StuI 4398 AGGCCT AGG^CCT
SwaI 808 ATTTAAAT ATTT^AAAT
Tth111I 3329 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 4577 CGCG CG^CG
4661 CGCG
AflIII 2586 ACATGT A^C[GA][CT]GT
2808 ACACGT
Asp700I 1689 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1758 GAAGAATTTC
AvaI 3399 CCCGGG C^[CT]CG[GA]G
4629 CCCGGG
BalI 196 TGGCCA TGG^CCA
3604 TGGCCA
BglII 3228 AGATCT A^GATCT
3649 AGATCT
BsaAI 1942 CACGTA [CT]AC^GT[GA]
2809 CACGTG
BseRI 2488 CTCCTC GAGGAG(10/8)
2533 CTCCTC
BsoBI 3399 CCCGGG C^[CT]CG[GA]G
4629 CCCGGG
BspMI 2925 ACCTGC ACCTGC(4/8)
2378 GCAGGT
BssSI 4488 CACGAG CACGAG(-5/-1)
410 CTCGTG
BstUI 4577 CGCG CG^CG
4661 CGCG
EcoRI 143 GAATTC G^AATTC
1206 GAATTC
FauI 1547 GCGGG CCCGC(4/6)
4611 GCGGG
MfeI 3680 CAATTG C^AATTG
5302 CAATTG
MluNI 196 TGGCCA TGG^CCA
3604 TGGCCA
MscI 196 TGGCCA TGG^CCA
3604 TGGCCA
MunI 3680 CAATTG C^AATTG
5302 CAATTG
MvnI 4577 CGCG CG^CG
4661 CGCG
NcoI 2703 CCATGG C^CATGG
5079 CCATGG
SmaI 3399 CCCGGG CCC^GGG
4629 CCCGGG
SphI 1139 GCATGC GCATG^C
4830 GCATGC
TaqI 4503 TCGA T^CGA
4654 TCGA
TthHB8I 4503 TCGA T^CGA
4654 TCGA
XmaCI 3399 CCCGGG C^CCGGG
4629 CCCGGG
XmaI 3399 CCCGGG C^CCGGG
4629 CCCGGG
XmnI 1689 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1758 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaII BanII BbsI BbvCI
BbvI BcnI BfaI BfrI BmyI
BpmI BpuAI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I BspHI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeI NdeII NheI
NlaIII NlaIV NsiI NspI PflMI
PleI PshBI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII