##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
AscI AseI AsnI Asp718I AviII
AvrII BamHI BbeI BclI BlnI
BsiEI BsiWI Bsp1407I BspDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI ScaI SfoI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 3519 GTCTAC GT^[AC][GT]AC
AcyI 3015 GACGCC G[GA]^CG[CT]C
AflII 5156 CTTAAG C^TTAAG
AspI 3806 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 640 CACGTG CAC^GTG
BfrI 5156 CTTAAG C^TTAAG
BsaAI 640 CACGTG [CT]AC^GT[GA]
BsaBI 1061 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3015 GACGCC G[GA]^CG[CT]C
BsmI 5366 GAATGC GAATGC(1/-1)
BspHI 3148 TCATGA T^CATGA
BspLU11I 3364 ACATGT A^CATGT
BsrBI 3820 CCGCTC CCGCTC(-3/-3)
EcoRI 1449 GAATTC G^AATTC
HaeII 2501 AGCGCC [GA]GCGC^[CT]
Hin1I 3015 GACGCC G[GA]^CG[CT]C
MamI 1061 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 4305 CATATG CA^TATG
PflFI 3806 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 640 CACGTG CAC^GTG
PmlI 640 CACGTG CAC^GTG
PshAI 4663 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3148 TCATGA T^CATGA
SbfI 1157 CCTGCAGG CCTGCA^GG
SexAI 12 ACCTGGT A^CC[AT]GGT
SmaI 5551 CCCGGG CCC^GGG
SphI 3235 GCATGC GCATG^C
Sse8387I 1157 CCTGCAGG CCTGCA^GG
StuI 1242 AGGCCT AGG^CCT
Tth111I 3806 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 5551 CCCGGG C^CCGGG
XmaI 5551 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 2221 CGCG CG^CG
4651 CGCG
AhdI 2625 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4002 GACTGCGAGTC
Asp700I 856 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
923 GAAACCCTTC
AspEI 2625 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4002 GACTGCGAGTC
BbsI 3803 GAAGAC GAAGAC(2/6)
1201 GTCTTC
BbvCI 3953 CCTCAGC CCTCAGC(-5/-2)
657 GCTGAGG
BcgI 2166 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4739 CGAGATCATTGC
BciVI 1621 GGATAC GTATCC(6/5)
3627 GGATAC
BglI 998 GCCCCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4581 GCCAAAGAGGC
BglII 3472 AGATCT A^GATCT
4714 AGATCT
BlpI 255 GCTCAGC GC^T[ACGT]AGC
2680 GCTTAGC
Bpu1102I 255 GCTCAGC GC^T[ACGT]AGC
2680 GCTTAGC
BpuAI 3803 GAAGAC GAAGAC(2/6)
1201 GTCTTC
BsrFI 4047 ACCGGC [GA]^CCGG[CT]
4562 ACCGGC
BssSI 4090 CTCGTG CACGAG(-5/-1)
4461 CTCGTG
BstUI 2221 CGCG CG^CG
4651 CGCG
CelII 255 GCTCAGC GC^T[ACGT]AGC
2680 GCTTAGC
Cfr10I 4047 ACCGGC [GA]^CCGG[CT]
4562 ACCGGC
Eam1105I 2625 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4002 GACTGCGAGTC
MvnI 2221 CGCG CG^CG
4651 CGCG
NcoI 963 CCATGG C^CATGG
3857 CCATGG
SfiI 997 GGCCCCGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4580 GGCCAAAGAGGCC
XcmI 2034 CCAGTGCTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3661 CCACAGATGGCTTGG
XmnI 856 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
923 GAAACCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccIII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DrdI DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NheI NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XhoII