##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI BcgI BciVI BclI
BfrI BlpI Bpu1102I BsaBI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DraIII Eam1105I
Eco47III EcoRV EcoT22I FbaI FseI
KpnI MamI MfeI MunI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AatII 3853 GACGTC GACGT^C
AccI 3961 GTCTAC GT^[AC][GT]AC
AflIII 4414 ACGCGT A^C[GA][CT]GT
Alw44I 4898 GTGCAC G^TGCAC
ApaLI 4898 GTGCAC G^TGCAC
Asp700I 4639 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 1823 CACGTG CAC^GTG
BglII 5473 AGATCT A^GATCT
BsaAI 1823 CACGTG [CT]AC^GT[GA]
BspHI 1457 TCATGA T^CATGA
BssSI 2411 CTCGTG CACGAG(-5/-1)
BstAPI 3285 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoRI 229 GAATTC G^AATTC
HincII 5374 GTTAAC GT[CT]^[GA]AC
HindII 5374 GTTAAC GT[CT]^[GA]AC
HindIII 4642 AAGCTT A^AGCTT
HpaI 5374 GTTAAC GTT^AAC
MluI 4414 ACGCGT A^CGCGT
NdeI 2779 CATATG CA^TATG
PmaCI 1823 CACGTG CAC^GTG
PmlI 1823 CACGTG CAC^GTG
RcaI 1457 TCATGA T^CATGA
SexAI 2027 ACCTGGT A^CC[AT]GGT
SphI 2478 GCATGC GCATG^C
SspI 4314 AATATT AAT^ATT
XmnI 4639 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 474 TCCGGA T^CCGGA
3000 TCCGGA
AvrII 8 CCTAGG C^CTAGG
2463 CCTAGG
BamHI 1633 GGATCC G^GATCC
2333 GGATCC
BbeI 108 GGCGCC GGCGC^C
4597 GGCGCC
BbsI 3685 GAAGAC GAAGAC(2/6)
4969 GTCTTC
BlnI 8 CCTAGG C^CTAGG
2463 CCTAGG
BpuAI 3685 GAAGAC GAAGAC(2/6)
4969 GTCTTC
BsaWI 474 TCCGGA [AT]^CCGG[AT]
3000 TCCGGA
BseAI 474 TCCGGA T^CCGGA
3000 TCCGGA
BsiEI 67 CGGCCG CG[GA][CT]^CG
103 CGGCCG
BsmBI 391 GAGACG CGTCTC(1/5)
3851 GAGACG
BspEI 474 TCCGGA T^CCGGA
3000 TCCGGA
BsrBI 1397 CCGCTC CCGCTC(-3/-3)
4321 CCGCTC
BsrDI 2732 CATTGC GCAATG(2/0)
5492 GCAATG
BsrFI 105 GCCGGC [GA]^CCGG[CT]
3281 GCCGGC
BstEII 3119 GGTCACC G^GT[ACGT]ACC
5403 GGTGACC
BstPI 3119 GGTCACC G^GT[ACGT]ACC
5403 GGTGACC
Cfr10I 105 GCCGGC [GA]^CCGG[CT]
3281 GCCGGC
EagI 67 CGGCCG C^GGCCG
103 CGGCCG
EclXI 67 CGGCCG C^GGCCG
103 CGGCCG
Eco52I 67 CGGCCG C^GGCCG
103 CGGCCG
EcoO65I 3119 GGTCACC G^GT[ACGT]ACC
5403 GGTGACC
KasI 108 GGCGCC G^GCGCC
4597 GGCGCC
KspI 55 CCGCGG CCGC^GG
3553 CCGCGG
MroI 474 TCCGGA T^CCGGA
3000 TCCGGA
NaeI 105 GCCGGC GCC^GGC
3281 GCCGGC
NarI 108 GGCGCC GG^CGCC
4597 GGCGCC
NcoI 2318 CCATGG C^CATGG
3608 CCATGG
NgoMIV 105 GCCGGC G^CCGGC
3281 GCCGGC
NspI 145 GCATGT [GA]CATG^[CT]
2478 GCATGC
SacI 541 GAGCTC GAGCT^C
3004 GAGCTC
SacII 55 CCGCGG CCGC^GG
3553 CCGCGG
SfoI 108 GGCGCC GGC^GCC
4597 GGCGCC
SmaI 1471 CCCGGG CCC^GGG
3538 CCCGGG
XbaI 573 TCTAGA T^CTAGA
2211 TCTAGA
XmaCI 1471 CCCGGG C^CCGGG
3538 CCCGGG
XmaI 1471 CCCGGG C^CCGGG
3538 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AspI AvaI AvaII AviII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflFI PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII