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# NO CUT ENZYMES ( 74 enzymes) #
##################################
AclI AflII AflIII AgeI Aor51HI
AscI AseI AsnI AviII AvrII
BbeI BfrI BlnI BsiEI BsiWI
BspHI BspLU11I BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
CpoI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FseI FspI
HaeII HincII HindII HpaI KasI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspI
SwaI TfiI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 385 GGTACC G^GTACC
AccII 3809 CGCG CG^CG
AccIII 4072 TCCGGA T^CCGGA
AhdI 1881 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1421 GTGCAC G^TGCAC
ApaLI 1421 GTGCAC G^TGCAC
Asp718I 385 GGTACC G^GTACC
AspEI 1881 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 5041 CGAGGGGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5152 GTATCC GTATCC(6/5)
BclI 3101 TGATCA T^GATCA
BsaBI 5390 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4072 TCCGGA [AT]^CCGG[AT]
BseAI 4072 TCCGGA T^CCGGA
BspDI 2562 ATCGAT AT^CGAT
BspEI 4072 TCCGGA T^CCGGA
BssSI 3509 CTCGTG CACGAG(-5/-1)
BstUI 3809 CGCG CG^CG
ClaI 2562 ATCGAT AT^CGAT
Eam1105I 1881 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 3101 TGATCA T^GATCA
KpnI 385 GGTACC GGTAC^C
KspI 3808 CCGCGG CCGC^GG
MamI 5390 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4072 TCCGGA T^CCGGA
MvnI 3809 CGCG CG^CG
SacII 3808 CCGCGG CCGC^GG
SmaI 2760 CCCGGG CCC^GGG
XmaCI 2760 CCCGGG C^CCGGG
XmaI 2760 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 4194 GACGTC GACGT^C
5235 GACGTC
AcyI 4194 GACGTC G[GA]^CG[CT]C
5235 GACGTC
AocI 407 CCTGAGG CC^T[ACGT]AGG
1677 CCTGAGG
Asp700I 600 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3905 GAAGATTTTC
AvaI 2760 CCCGGG C^[CT]CG[GA]G
5039 CCCGAG
BamHI 1134 GGATCC G^GATCC
2920 GGATCC
BsaHI 4194 GACGTC G[GA]^CG[CT]C
5235 GACGTC
BsaI 160 GGTCTC GGTCTC(1/5)
1713 GGTCTC
BsmI 632 GAATGC GAATGC(1/-1)
2226 GAATGC
BsoBI 2760 CCCGGG C^[CT]CG[GA]G
5039 CCCGAG
Bsp1407I 799 TGTACA T^GTACA
2050 TGTACA
BsrBI 2616 CCGCTC CCGCTC(-3/-3)
5129 GAGCGG
BsrDI 2328 CATTGC GCAATG(2/0)
3826 GCAATG
BsrGI 799 TGTACA T^GTACA
2050 TGTACA
Bsu36I 407 CCTGAGG CC^T[ACGT]AGG
1677 CCTGAGG
DrdI 4005 GACATGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5412 GACGGCATGGTC
Eco81I 407 CCTGAGG CC^T[ACGT]AGG
1677 CCTGAGG
EcoT22I 2325 ATGCAT ATGCA^T
2848 ATGCAT
HgaI 3305 GACGC GACGC(5/10)
5103 GCGTC
Hin1I 4194 GACGTC G[GA]^CG[CT]C
5235 GACGTC
HindIII 3656 AAGCTT A^AGCTT
4118 AAGCTT
NsiI 2325 ATGCAT ATGCA^T
2848 ATGCAT
SapI 1187 GAAGAGC GCTCTTC(1/4)
4154 GAAGAGC
SbfI 1025 CCTGCAGG CCTGCA^GG
3566 CCTGCAGG
SexAI 3340 ACCTGGT A^CC[AT]GGT
4066 ACCTGGT
SphI 1040 GCATGC GCATG^C
4270 GCATGC
Sse8387I 1025 CCTGCAGG CCTGCA^GG
3566 CCTGCAGG
SspBI 799 TGTACA T^GTACA
2050 TGTACA
XmnI 600 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3905 GAAGATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AspI AvaII BalI BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII