##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AclI AflII AgeI AseI AsnI
AviII AvrII BbsI BfrI BlnI
BpuAI BsaBI BsiWI BspDI BspLU11I
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI EagI EclXI Eco52I
EcoRI EcoT22I FseI FspI HpaI
KspI MamI MfeI MluI MunI
NdeI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
RsrII SacII SalI ScaI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 4448 GACGTC GACGT^C
AflIII 3250 ACACGT A^C[GA][CT]GT
AscI 27 GGCGCGCC GG^CGCGCC
AspI 4596 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2934 GGATCC G^GATCC
BbeI 72 GGCGCC GGCGC^C
BcgI 3932 CGAAAGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1645 TGATCA T^GATCA
BglII 3874 AGATCT A^GATCT
BlpI 617 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 617 GCTGAGC GC^T[ACGT]AGC
BsiEI 4730 CGATCG CG[GA][CT]^CG
BsmBI 1639 CGTCTC CGTCTC(1/5)
Bsp1407I 3799 TGTACA T^GTACA
BspHI 1963 TCATGA T^CATGA
BsrGI 3799 TGTACA T^GTACA
BssHII 28 GCGCGC G^CGCGC
BstEII 3205 GGTCACC G^GT[ACGT]ACC
BstPI 3205 GGTCACC G^GT[ACGT]ACC
CelII 617 GCTGAGC GC^T[ACGT]AGC
DraIII 1338 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 3205 GGTCACC G^GT[ACGT]ACC
EcoRV 2645 GATATC GAT^ATC
FbaI 1645 TGATCA T^GATCA
HincII 5028 GTCAAC GT[CT]^[GA]AC
HindII 5028 GTCAAC GT[CT]^[GA]AC
KasI 72 GGCGCC G^GCGCC
NarI 72 GGCGCC GG^CGCC
NheI 355 GCTAGC G^CTAGC
NspI 4366 GCATGC [GA]CATG^[CT]
PaeR7I 4608 CTCGAG C^TCGAG
PflFI 4596 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1859 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 4730 CGATCG CGAT^CG
RcaI 1963 TCATGA T^CATGA
SapI 4320 GAAGAGC GCTCTTC(1/4)
SfoI 72 GGCGCC GGC^GCC
SphI 4366 GCATGC GCATG^C
SspBI 3799 TGTACA T^GTACA
Tth111I 4596 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4608 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 3224 GTAGAC GT^[AC][GT]AC
5114 GTAGAC
AccIII 885 TCCGGA T^CCGGA
1115 TCCGGA
AcsI 4458 AAATTT [GA]^AATT[CT]
4738 GAATTT
AhdI 50 GACCAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2516 GACACCTTGTC
Alw44I 641 GTGCAC G^TGCAC
4116 GTGCAC
Aor51HI 2564 AGCGCT AGC^GCT
2706 AGCGCT
ApaLI 641 GTGCAC G^TGCAC
4116 GTGCAC
ApoI 4458 AAATTT [GA]^AATT[CT]
4738 GAATTT
Asp700I 3473 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4738 GAATTTCTTC
AspEI 50 GACCAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2516 GACACCTTGTC
BbrPI 2408 CACGTG CAC^GTG
3251 CACGTG
BciVI 133 GTATCC GTATCC(6/5)
4929 GTATCC
BsaI 3834 GAGACC GGTCTC(1/5)
3906 GAGACC
BseAI 885 TCCGGA T^CCGGA
1115 TCCGGA
BspEI 885 TCCGGA T^CCGGA
1115 TCCGGA
BssSI 3 CACGAG CACGAG(-5/-1)
4119 CACGAG
BstXI 159 CCACCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
286 CCAATACAGTGG
Eam1105I 50 GACCAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2516 GACACCTTGTC
Eco47III 2564 AGCGCT AGC^GCT
2706 AGCGCT
HindIII 1352 AAGCTT A^AGCTT
2497 AAGCTT
MroI 885 TCCGGA T^CCGGA
1115 TCCGGA
NaeI 2828 GCCGGC GCC^GGC
4370 GCCGGC
NgoMIV 2828 GCCGGC G^CCGGC
4370 GCCGGC
PmaCI 2408 CACGTG CAC^GTG
3251 CACGTG
PmlI 2408 CACGTG CAC^GTG
3251 CACGTG
PpuMI 790 AGGACCT [GA]G^G[AT]CC[CT]
2079 GGGACCC
SbfI 2530 CCTGCAGG CCTGCA^GG
4836 CCTGCAGG
SexAI 1186 ACCTGGT A^CC[AT]GGT
1368 ACCTGGT
SfiI 1541 GGCCCAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4339 GGCCTCCGTGGCC
SmaI 3468 CCCGGG CCC^GGG
4335 CCCGGG
Sse8387I 2530 CCTGCAGG CCTGCA^GG
4836 CCTGCAGG
XmaCI 3468 CCCGGG C^CCGGG
4335 CCCGGG
XmaI 3468 CCCGGG C^CCGGG
4335 CCCGGG
XmnI 3473 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4738 GAATTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII