################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AclI AflII AgeI AseI AsnI AviII AvrII BbsI BfrI BlnI BpuAI BsaBI BsiWI BspDI BspLU11I Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI EagI EclXI Eco52I EcoRI EcoT22I FseI FspI HpaI KspI MamI MfeI MluI MunI NdeI NotI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I RsrII SacII SalI ScaI SfuI SgrAI SnaBI SpeI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 4448 GACGTC GACGT^C AflIII 3250 ACACGT A^C[GA][CT]GT AscI 27 GGCGCGCC GG^CGCGCC AspI 4596 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2934 GGATCC G^GATCC BbeI 72 GGCGCC GGCGC^C BcgI 3932 CGAAAGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1645 TGATCA T^GATCA BglII 3874 AGATCT A^GATCT BlpI 617 GCTGAGC GC^T[ACGT]AGC Bpu1102I 617 GCTGAGC GC^T[ACGT]AGC BsiEI 4730 CGATCG CG[GA][CT]^CG BsmBI 1639 CGTCTC CGTCTC(1/5) Bsp1407I 3799 TGTACA T^GTACA BspHI 1963 TCATGA T^CATGA BsrGI 3799 TGTACA T^GTACA BssHII 28 GCGCGC G^CGCGC BstEII 3205 GGTCACC G^GT[ACGT]ACC BstPI 3205 GGTCACC G^GT[ACGT]ACC CelII 617 GCTGAGC GC^T[ACGT]AGC DraIII 1338 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 3205 GGTCACC G^GT[ACGT]ACC EcoRV 2645 GATATC GAT^ATC FbaI 1645 TGATCA T^GATCA HincII 5028 GTCAAC GT[CT]^[GA]AC HindII 5028 GTCAAC GT[CT]^[GA]AC KasI 72 GGCGCC G^GCGCC NarI 72 GGCGCC GG^CGCC NheI 355 GCTAGC G^CTAGC NspI 4366 GCATGC [GA]CATG^[CT] PaeR7I 4608 CTCGAG C^TCGAG PflFI 4596 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1859 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 4730 CGATCG CGAT^CG RcaI 1963 TCATGA T^CATGA SapI 4320 GAAGAGC GCTCTTC(1/4) SfoI 72 GGCGCC GGC^GCC SphI 4366 GCATGC GCATG^C SspBI 3799 TGTACA T^GTACA Tth111I 4596 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 4608 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccI 3224 GTAGAC GT^[AC][GT]AC 5114 GTAGAC AccIII 885 TCCGGA T^CCGGA 1115 TCCGGA AcsI 4458 AAATTT [GA]^AATT[CT] 4738 GAATTT AhdI 50 GACCAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2516 GACACCTTGTC Alw44I 641 GTGCAC G^TGCAC 4116 GTGCAC Aor51HI 2564 AGCGCT AGC^GCT 2706 AGCGCT ApaLI 641 GTGCAC G^TGCAC 4116 GTGCAC ApoI 4458 AAATTT [GA]^AATT[CT] 4738 GAATTT Asp700I 3473 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4738 GAATTTCTTC AspEI 50 GACCAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2516 GACACCTTGTC BbrPI 2408 CACGTG CAC^GTG 3251 CACGTG BciVI 133 GTATCC GTATCC(6/5) 4929 GTATCC BsaI 3834 GAGACC GGTCTC(1/5) 3906 GAGACC BseAI 885 TCCGGA T^CCGGA 1115 TCCGGA BspEI 885 TCCGGA T^CCGGA 1115 TCCGGA BssSI 3 CACGAG CACGAG(-5/-1) 4119 CACGAG BstXI 159 CCACCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 286 CCAATACAGTGG Eam1105I 50 GACCAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2516 GACACCTTGTC Eco47III 2564 AGCGCT AGC^GCT 2706 AGCGCT HindIII 1352 AAGCTT A^AGCTT 2497 AAGCTT MroI 885 TCCGGA T^CCGGA 1115 TCCGGA NaeI 2828 GCCGGC GCC^GGC 4370 GCCGGC NgoMIV 2828 GCCGGC G^CCGGC 4370 GCCGGC PmaCI 2408 CACGTG CAC^GTG 3251 CACGTG PmlI 2408 CACGTG CAC^GTG 3251 CACGTG PpuMI 790 AGGACCT [GA]G^G[AT]CC[CT] 2079 GGGACCC SbfI 2530 CCTGCAGG CCTGCA^GG 4836 CCTGCAGG SexAI 1186 ACCTGGT A^CC[AT]GGT 1368 ACCTGGT SfiI 1541 GGCCCAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 4339 GGCCTCCGTGGCC SmaI 3468 CCCGGG CCC^GGG 4335 CCCGGG Sse8387I 2530 CCTGCAGG CCTGCA^GG 4836 CCTGCAGG XmaCI 3468 CCCGGG C^CCGGG 4335 CCCGGG XmaI 3468 CCCGGG C^CCGGG 4335 CCCGGG XmnI 3473 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4738 GAATTTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### Acc65I AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI Asp718I AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII