##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AccIII AflII AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AviII
BciVI BfrI BseAI BsiWI BspEI
BssHII CpoI DraI Eam1105I Eco47III
EcoRV EcoT22I FseI FspI HindIII
KspI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
PacI PinAI PmeI PshBI RsrII
SacI SacII SbfI SgrAI SphI
Sse8387I SspI SwaI TfiI XbaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 4625 GGTACC G^GTACC
AclI 6477 AACGTT AA^CGTT
AcsI 6786 GAATTC [GA]^AATT[CT]
ApoI 6786 GAATTC [GA]^AATT[CT]
Asp718I 4625 GGTACC G^GTACC
AspI 5388 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 6710 CCTAGG C^CTAGG
BlnI 6710 CCTAGG C^CTAGG
BsaBI 5537 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 5812 GCATTC GAATGC(1/-1)
BspLU11I 3399 ACATGT A^CATGT
BstBI 1734 TTCGAA TT^CGAA
EcoRI 6786 GAATTC G^AATTC
HpaI 2120 GTTAAC GTT^AAC
KpnI 4625 GGTACC GGTAC^C
MamI 5537 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2121 TTAA T^TAA
NspV 1734 TTCGAA TT^CGAA
PflFI 5388 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4044 GACGGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 6477 AACGTT AA^CGTT
PvuI 7102 CGATCG CGAT^CG
SalI 99 GTCGAC G^TCGAC
SapI 7736 GAAGAGC GCTCTTC(1/4)
ScaI 4604 AGTACT AGT^ACT
SexAI 6070 ACCTGGT A^CC[AT]GGT
SfuI 1734 TTCGAA TT^CGAA
SnaBI 7725 TACGTA TAC^GTA
SpeI 8230 ACTAGT A^CTAGT
Tru9I 2121 TTAA T^TAA
Tth111I 5388 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 355 ACGTGT A^C[GA][CT]GT
3399 ACATGT
Asp700I 176 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6954 GAACACATTC
BbeI 633 GGCGCC GGCGC^C
3318 GGCGCC
BsaWI 3804 TCCGGT [AT]^CCGG[AT]
3900 ACCGGA
BspDI 4746 ATCGAT AT^CGAT
7104 ATCGAT
BsrBI 617 GAGCGG CCGCTC(-3/-3)
3549 GAGCGG
Bst1107I 2358 GTATAC GTA^TAC
4847 GTATAC
BstAPI 5071 GCACCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6068 GCACCTGGTGC
BstZ17I 2358 GTATAC GTA^TAC
4847 GTATAC
ClaI 4746 ATCGAT AT^CGAT
7104 ATCGAT
EcoNI 140 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
7868 CCTGCTCAAGG
KasI 633 GGCGCC G^GCGCC
3318 GGCGCC
NarI 633 GGCGCC GG^CGCC
3318 GGCGCC
SfiI 3920 GGCCCGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
8043 GGCCGCCCTGGCC
SfoI 633 GGCGCC GGC^GCC
3318 GGCGCC
XmnI 176 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6954 GAACACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 161 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbrPI BbsI BbvCI BbvI BcgI
BclI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrDI BsrFI
BsrGI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DrdI DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PaeR7I
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspBI StuI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoI XhoII XmaCI
XmaI