##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccIII AflII AflIII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AviII BbeI BcgI BciVI BfrI
BseAI BsiWI BspEI BspLU11I BsrBI
BssHII BstBI CpoI DraI Eam1105I
Eco47III EcoRV EcoT22I FseI FspI
HindIII HpaI KasI KspI MfeI
MluI MroI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
RsrII SacI SacII SalI SbfI
SfoI SfuI SgrAI SphI Sse8387I
SspI SwaI TfiI Tru9I XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 3632 GACGTC GACGT^C
Acc65I 1036 GGTACC G^GTACC
AclI 2888 AACGTT AA^CGTT
AcsI 3197 GAATTC [GA]^AATT[CT]
ApoI 3197 GAATTC [GA]^AATT[CT]
Asp700I 3365 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1036 GGTACC G^GTACC
AspI 1799 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3121 CCTAGG C^CTAGG
BamHI 1204 GGATCC G^GATCC
BbvCI 2908 CCTCAGC CCTCAGC(-5/-2)
BlnI 3121 CCTAGG C^CTAGG
BsaBI 1948 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2223 GCATTC GAATGC(1/-1)
BspHI 1075 TCATGA T^CATGA
Bst1107I 1258 GTATAC GTA^TAC
BstZ17I 1258 GTATAC GTA^TAC
EagI 60 CGGCCG C^GGCCG
EclXI 60 CGGCCG C^GGCCG
Eco52I 60 CGGCCG C^GGCCG
EcoNI 4279 CCTGCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3197 GAATTC G^AATTC
HaeII 4577 GGCGCT [GA]GCGC^[CT]
KpnI 1036 GGTACC GGTAC^C
MamI 1948 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 4 CTCGAG C^TCGAG
PflFI 1799 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 455 GACGGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2888 AACGTT AA^CGTT
PvuI 3513 CGATCG CGAT^CG
RcaI 1075 TCATGA T^CATGA
SapI 4147 GAAGAGC GCTCTTC(1/4)
ScaI 1015 AGTACT AGT^ACT
SexAI 2481 ACCTGGT A^CC[AT]GGT
SnaBI 4136 TACGTA TAC^GTA
SpeI 4641 ACTAGT A^CTAGT
Tth111I 1799 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4 CTCGAG C^TCGAG
XmnI 3365 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BclI 1437 TGATCA T^GATCA
2538 TGATCA
BglII 36 AGATCT A^GATCT
3270 AGATCT
BlpI 2662 GCTCAGC GC^T[ACGT]AGC
4123 GCTGAGC
Bpu1102I 2662 GCTCAGC GC^T[ACGT]AGC
4123 GCTGAGC
BsaWI 215 TCCGGT [AT]^CCGG[AT]
311 ACCGGA
BspDI 1157 ATCGAT AT^CGAT
3515 ATCGAT
BsrFI 4257 GCCGGC [GA]^CCGG[CT]
4387 GCCGGC
BstAPI 1482 GCACCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2479 GCACCTGGTGC
CelII 2662 GCTCAGC GC^T[ACGT]AGC
4123 GCTGAGC
Cfr10I 4257 GCCGGC [GA]^CCGG[CT]
4387 GCCGGC
ClaI 1157 ATCGAT AT^CGAT
3515 ATCGAT
DrdI 784 GACAGATGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3335 GACTACGAAGTC
FbaI 1437 TGATCA T^GATCA
2538 TGATCA
NaeI 4257 GCCGGC GCC^GGC
4387 GCCGGC
NcoI 2053 CCATGG C^CATGG
3891 CCATGG
NgoMIV 4257 GCCGGC G^CCGGC
4387 GCCGGC
PflMI 2506 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4568 CCATTTGGTGG
SfiI 331 GGCCCGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4454 GGCCGCCCTGGCC
SmlI 4 CTCGAG C^T[CT][GA]AG
4283 CTCAAG
Van91I 2506 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4568 CCATTTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SspBI StuI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI