# /usr/local/bin/fasta34_t -T 8 -b50 -d10 -E0.01 -H -O./tmp/as00108.fasta.nr -Q ../query/FLJ00108.ptfa /cdna2/lib/nr/nr 2 FASTA searches a protein or DNA sequence data bank version 34.26.5 April 26, 2007 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 FLJ00108, 216 aa vs /cdna2/lib/nr/nr library 2693465022 residues in 7827732 sequences statistics sampled from 60000 to 7826556 sequences Expectation_n fit: rho(ln(x))= 4.9988+/-0.000185; mu= 10.0487+/- 0.010 mean_var=78.6239+/-15.558, 0's: 33 Z-trim: 35 B-trim: 2108 in 1/64 Lambda= 0.144643 FASTA (3.5 Sept 2006) function [optimized, BL50 matrix (15:-5)] ktup: 2 join: 36, opt: 24, open/ext: -10/-2, width: 16 The best scores are: opt bits E(7827732) gi|10440518|dbj|BAB15789.1| FLJ00108 protein [Homo ( 216) 1483 318.1 6.4e-85 gi|119603011|gb|EAW82605.1| spondin 2, extracellul ( 353) 1466 314.8 1.1e-83 gi|114592789|ref|XP_001141566.1| PREDICTED: spondi ( 289) 533 120.0 3.7e-25 gi|52783469|sp|Q9BUD6.1|SPON2_HUMAN RecName: Full= ( 331) 533 120.0 4.1e-25 gi|6172221|dbj|BAA85892.1| spondin 2 [Homo sapiens ( 331) 533 120.0 4.1e-25 gi|119603010|gb|EAW82604.1| spondin 2, extracellul ( 331) 533 120.0 4.1e-25 gi|55725252|emb|CAH89491.1| hypothetical protein [ ( 331) 533 120.0 4.1e-25 gi|114592773|ref|XP_001141883.1| PREDICTED: hypoth ( 331) 533 120.0 4.1e-25 gi|63993758|gb|AAY40988.1| unknown [Homo sapiens] ( 331) 533 120.0 4.1e-25 gi|108995245|ref|XP_001092523.1| PREDICTED: simila ( 331) 509 115.0 1.3e-23 gi|52783424|sp|Q9WV75.1|SPON2_RAT RecName: Full=Sp ( 330) 481 109.2 7.6e-22 gi|87578313|gb|AAI13292.1| Spondin 2, extracellula ( 332) 477 108.3 1.4e-21 gi|47847418|dbj|BAD21381.1| mFLJ00108 protein [Mus ( 289) 476 108.1 1.4e-21 gi|17160895|gb|AAH17616.1| Spondin 2, extracellula ( 330) 476 108.1 1.6e-21 gi|26324944|dbj|BAC26226.1| unnamed protein produc ( 330) 476 108.1 1.6e-21 gi|52783454|sp|Q8BMS2.2|SPON2_MOUSE RecName: Full= ( 330) 476 108.1 1.6e-21 gi|149640610|ref|XP_001510812.1| PREDICTED: hypoth ( 332) 471 107.1 3.2e-21 gi|126332264|ref|XP_001376130.1| PREDICTED: simila ( 489) 453 103.5 5.8e-20 gi|118090805|ref|XP_420847.2| PREDICTED: similar t ( 330) 450 102.7 6.7e-20 gi|111308033|gb|AAI21779.1| Spon2b protein [Danio ( 331) 433 99.2 7.9e-19 gi|209154848|gb|ACI33656.1| Spondin-2 precursor [S ( 339) 430 98.5 1.2e-18 gi|148705465|gb|EDL37412.1| spondin 2, extracellul ( 202) 424 97.1 2e-18 gi|47214176|emb|CAF96977.1| unnamed protein produc ( 355) 420 96.5 5.4e-18 gi|50418027|gb|AAH77984.1| Spon2-prov protein [Xen ( 313) 419 96.2 5.7e-18 gi|2529223|dbj|BAA22809.1| MINDIN2 [Danio rerio] ( 331) 415 95.4 1.1e-17 gi|112418592|gb|AAI22079.1| Hypothetical protein M ( 313) 412 94.8 1.6e-17 gi|47214367|emb|CAG01212.1| unnamed protein produc ( 280) 370 86.0 6.3e-15 gi|190339330|gb|AAI62543.1| Spondin 2a, extracellu ( 334) 355 82.9 6.3e-14 gi|2529221|dbj|BAA22808.1| MINDIN1 [Danio rerio] ( 334) 353 82.5 8.4e-14 gi|210105619|gb|EEA53626.1| hypothetical protein B ( 679) 329 77.8 4.5e-12 gi|210105616|gb|EEA53623.1| hypothetical protein B ( 693) 329 77.8 4.6e-12 gi|210096511|gb|EEA44655.1| hypothetical protein B ( 321) 316 74.7 1.7e-11 gi|2130613|gb|AAB58341.1| M-spondin [Drosophila me ( 598) 319 75.6 1.8e-11 gi|190658787|gb|EDV56000.1| GG22407 [Drosophila er ( 589) 318 75.4 2e-11 gi|194125852|gb|EDW47895.1| GM20193 [Drosophila se ( 590) 318 75.4 2e-11 gi|194193543|gb|EDX07119.1| GD25664 [Drosophila si ( 593) 318 75.4 2e-11 gi|198135927|gb|EAL25189.2| GA10108 [Drosophila ps ( 619) 318 75.4 2.1e-11 gi|72040205|ref|XP_794178.1| PREDICTED: similar to ( 418) 316 74.8 2.1e-11 gi|194158857|gb|EDW73758.1| GK19519 [Drosophila wi ( 573) 317 75.2 2.3e-11 gi|194177444|gb|EDW91055.1| GE12296 [Drosophila ya ( 597) 315 74.8 3.1e-11 gi|7303154|gb|AAF58219.1| M-spondin [Drosophila me ( 601) 315 74.8 3.1e-11 gi|193899213|gb|EDV98079.1| GH22917 [Drosophila gr ( 600) 311 73.9 5.6e-11 gi|193911843|gb|EDW10710.1| GI18408 [Drosophila mo ( 593) 310 73.7 6.4e-11 gi|190622058|gb|EDV37582.1| GF13520 [Drosophila an ( 597) 310 73.7 6.4e-11 gi|194144050|gb|EDW60446.1| GJ21488 [Drosophila vi ( 601) 310 73.7 6.5e-11 gi|91078104|ref|XP_972867.1| PREDICTED: similar to ( 446) 297 70.9 3.4e-10 gi|194110031|gb|EDW32074.1| GL10630 [Drosophila pe (1190) 296 71.1 8e-10 gi|212512404|gb|EEB15182.1| f-spondin, putative [P ( 397) 287 68.8 1.3e-09 gi|215501491|gb|EEC10985.1| f-spondin, putative [I ( 191) 279 66.8 2.5e-09 gi|3319874|emb|CAA06854.1| F-spondin [Branchiostom ( 898) 283 68.3 4.3e-09 >>gi|10440518|dbj|BAB15789.1| FLJ00108 protein [Homo sap (216 aa) initn: 1483 init1: 1483 opt: 1483 Z-score: 1680.9 bits: 318.1 E(): 6.4e-85 Smith-Waterman score: 1483; 100.000% identity (100.000% similar) in 216 aa overlap (1-216:1-216) 10 20 30 40 50 60 FLJ001 SAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGDRWREQAALDLYPYDAGTDSGFT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: gi|104 SAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGDRWREQAALDLYPYDAGTDSGFT 10 20 30 40 50 60 70 80 90 100 110 120 FLJ001 FSSPNFATIPQDTVTEVSGHPACARAAMAPAVGTGLGPGCTLASQPRTAACGFCVEEALL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: gi|104 FSSPNFATIPQDTVTEVSGHPACARAAMAPAVGTGLGPGCTLASQPRTAACGFCVEEALL 70 80 90 100 110 120 130 140 150 160 170 180 FLJ001 LAPAVSKGVQPSHTLPTRGHSTAGRTAGRASRGGRPFLASVSSPCSVGWLRGVHGTLSPT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: gi|104 LAPAVSKGVQPSHTLPTRGHSTAGRTAGRASRGGRPFLASVSSPCSVGWLRGVHGTLSPT 130 140 150 160 170 180 190 200 210 FLJ001 VVQRVRWTLGSGPSEAIRIPEGGLLGPQANLFFLPR :::::::::::::::::::::::::::::::::::: gi|104 VVQRVRWTLGSGPSEAIRIPEGGLLGPQANLFFLPR 190 200 210 >>gi|119603011|gb|EAW82605.1| spondin 2, extracellular m (353 aa) initn: 1494 init1: 1450 opt: 1466 Z-score: 1658.9 bits: 314.8 E(): 1.1e-83 Smith-Waterman score: 1466; 99.078% identity (99.539% similar) in 217 aa overlap (1-216:137-353) 10 20 30 FLJ001 SAELEVQRRHSLVSFVVRIVPSPDWFVGVD :::::::::::::::::::::::::::::: gi|119 MKEIEAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVD 110 120 130 140 150 160 40 50 60 70 80 90 FLJ001 SLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEVSGHPACARAAMAP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: gi|119 SLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEVSGHPACARAAMAP 170 180 190 200 210 220 100 110 120 130 140 150 FLJ001 AVGTGLGPGCTLASQPRTAACGFCVEEALLLAPAVSKGVQPSHTLPTRGHSTAGRTAGRA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: gi|119 AVGTGLGPGCTLASQPRTAACGFCVEEALLLAPAVSKGVQPSHTLPTRGHSTAGRTAGRA 230 240 250 260 270 280 160 170 180 190 200 210 FLJ001 SRGGRPFLASVSSPCSVGWLRGVHGTLSPTVVQRVRWTLGSGPSEAIRIPEGGLLGPQAN ::::::::::::::::::::::::::::::::::.::::::::::::::::::::::::: gi|119 SRGGRPFLASVSSPCSVGWLRGVHGTLSPTVVQRARWTLGSGPSEAIRIPEGGLLGPQAN 290 300 310 320 330 340 FLJ001 L-FFLPR : ::::: gi|119 LLFFLPR 350 >>gi|114592789|ref|XP_001141566.1| PREDICTED: spondin 2, (289 aa) initn: 549 init1: 526 opt: 533 Z-score: 607.8 bits: 120.0 E(): 3.7e-25 Smith-Waterman score: 533; 91.860% identity (95.349% similar) in 86 aa overlap (1-82:137-222) 10 20 30 FLJ001 SAELEVQRRHSLVSFVVRIVPSPDWFVGVD :::::::::::::::::::::::::::::: gi|114 MKEIEAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVD 110 120 130 140 150 160 40 50 60 70 80 FLJ001 SLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEVSG----HPACARA ::::::::::::::::::::::::::::::::::::::::::::::... ::: gi|114 SLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFY 170 180 190 200 210 220 90 100 110 120 130 140 FLJ001 AMAPAVGTGLGPGCTLASQPRTAACGFCVEEALLLAPAVSKGVQPSHTLPTRGHSTAGRT gi|114 YPRLKALPPIARVTLVRLRQSPRAFIPPAPVLPSRDNEIVDSASGNGHTGHTGHTAAPNP 230 240 250 260 270 280 >>gi|52783469|sp|Q9BUD6.1|SPON2_HUMAN RecName: Full=Spon (331 aa) initn: 549 init1: 526 opt: 533 Z-score: 607.1 bits: 120.0 E(): 4.1e-25 Smith-Waterman score: 533; 91.860% identity (95.349% similar) in 86 aa overlap (1-82:137-222) 10 20 30 FLJ001 SAELEVQRRHSLVSFVVRIVPSPDWFVGVD :::::::::::::::::::::::::::::: gi|527 MKEIEAAGEALQSVHEVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVD 110 120 130 140 150 160 40 50 60 70 80 FLJ001 SLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEVSG----HPACARA ::::::::::::::::::::::::::::::::::::::::::::::... ::: gi|527 SLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFY 170 180 190 200 210 220 90 100 110 120 130 140 FLJ001 AMAPAVGTGLGPGCTLASQPRTAACGFCVEEALLLAPAVSKGVQPSHTLPTRGHSTAGRT gi|527 YPRLKALPPIARVTLLRLRQSPRAFIPPAPVLPSRDNEIVDSASVPETPLDCEVSLWSSW 230 240 250 260 270 280 >>gi|6172221|dbj|BAA85892.1| spondin 2 [Homo sapiens] (331 aa) initn: 549 init1: 526 opt: 533 Z-score: 607.1 bits: 120.0 E(): 4.1e-25 Smith-Waterman score: 533; 91.860% identity (95.349% similar) in 86 aa overlap (1-82:137-222) 10 20 30 FLJ001 SAELEVQRRHSLVSFVVRIVPSPDWFVGVD :::::::::::::::::::::::::::::: gi|617 MKEIEAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVD 110 120 130 140 150 160 40 50 60 70 80 FLJ001 SLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEVSG----HPACARA ::::::::::::::::::::::::::::::::::::::::::::::... ::: gi|617 SLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFY 170 180 190 200 210 220 90 100 110 120 130 140 FLJ001 AMAPAVGTGLGPGCTLASQPRTAACGFCVEEALLLAPAVSKGVQPSHTLPTRGHSTAGRT gi|617 YPRLKALPPIARVTLVRLRQSPRAFIPPAPVLPSRDNEIVDSASVPETPLDCEVSLWSSW 230 240 250 260 270 280 >>gi|119603010|gb|EAW82604.1| spondin 2, extracellular m (331 aa) initn: 549 init1: 526 opt: 533 Z-score: 607.1 bits: 120.0 E(): 4.1e-25 Smith-Waterman score: 533; 91.860% identity (95.349% similar) in 86 aa overlap (1-82:137-222) 10 20 30 FLJ001 SAELEVQRRHSLVSFVVRIVPSPDWFVGVD :::::::::::::::::::::::::::::: gi|119 MKEIEAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVD 110 120 130 140 150 160 40 50 60 70 80 FLJ001 SLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEVSG----HPACARA ::::::::::::::::::::::::::::::::::::::::::::::... ::: gi|119 SLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFY 170 180 190 200 210 220 90 100 110 120 130 140 FLJ001 AMAPAVGTGLGPGCTLASQPRTAACGFCVEEALLLAPAVSKGVQPSHTLPTRGHSTAGRT gi|119 YPRLKALPPIARVTLLRLRQSPRAFIPPAPVLPSRDNEIVDSASVPETPLDCEVSLWSSW 230 240 250 260 270 280 >>gi|55725252|emb|CAH89491.1| hypothetical protein [Pong (331 aa) initn: 526 init1: 526 opt: 533 Z-score: 607.1 bits: 120.0 E(): 4.1e-25 Smith-Waterman score: 533; 91.860% identity (95.349% similar) in 86 aa overlap (1-82:137-222) 10 20 30 FLJ001 SAELEVQRRHSLVSFVVRIVPSPDWFVGVD :::::::::::::::::::::::::::::: gi|557 MKEIEAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVD 110 120 130 140 150 160 40 50 60 70 80 FLJ001 SLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEVSG----HPACARA ::::::::::::::::::::::::::::::::::::::::::::::... ::: gi|557 SLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFH 170 180 190 200 210 220 90 100 110 120 130 140 FLJ001 AMAPAVGTGLGPGCTLASQPRTAACGFCVEEALLLAPAVSKGVQPSHTLPTRGHSTAGRT gi|557 YPRLKALPPIARVTLVRLRQSPRAFVPPAPVLPSRDNEIVDSASVPETPLDCEVSLWSSW 230 240 250 260 270 280 >>gi|114592773|ref|XP_001141883.1| PREDICTED: hypothetic (331 aa) initn: 549 init1: 526 opt: 533 Z-score: 607.1 bits: 120.0 E(): 4.1e-25 Smith-Waterman score: 533; 91.860% identity (95.349% similar) in 86 aa overlap (1-82:137-222) 10 20 30 FLJ001 SAELEVQRRHSLVSFVVRIVPSPDWFVGVD :::::::::::::::::::::::::::::: gi|114 MKEIEAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVD 110 120 130 140 150 160 40 50 60 70 80 FLJ001 SLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEVSG----HPACARA ::::::::::::::::::::::::::::::::::::::::::::::... ::: gi|114 SLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFY 170 180 190 200 210 220 90 100 110 120 130 140 FLJ001 AMAPAVGTGLGPGCTLASQPRTAACGFCVEEALLLAPAVSKGVQPSHTLPTRGHSTAGRT gi|114 YPRLKALPPIARVTLVRLRQSPRAFIPPAPVLPSRDNEIVDSASVPETPLDCEVSLWSSW 230 240 250 260 270 280 >>gi|63993758|gb|AAY40988.1| unknown [Homo sapiens] (331 aa) initn: 549 init1: 526 opt: 533 Z-score: 607.1 bits: 120.0 E(): 4.1e-25 Smith-Waterman score: 533; 91.860% identity (95.349% similar) in 86 aa overlap (1-82:137-222) 10 20 30 FLJ001 SAELEVQRRHSLVSFVVRIVPSPDWFVGVD :::::::::::::::::::::::::::::: gi|639 MKEIEAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVD 110 120 130 140 150 160 40 50 60 70 80 FLJ001 SLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEVSG----HPACARA ::::::::::::::::::::::::::::::::::::::::::::::... ::: gi|639 SLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFY 170 180 190 200 210 220 90 100 110 120 130 140 FLJ001 AMAPAVGTGLGPGCTLASQPRTAACGFCVEEALLLAPAVSKGVQPSHTLPTRGHSTAGRT gi|639 YPRLKALPPIARVTLVRLRQSPRAFIPPAPVLPSRDNEIVDSASVPETPLDCEVSLWSSW 230 240 250 260 270 280 >>gi|108995245|ref|XP_001092523.1| PREDICTED: similar to (331 aa) initn: 525 init1: 502 opt: 509 Z-score: 580.0 bits: 115.0 E(): 1.3e-23 Smith-Waterman score: 509; 89.535% identity (94.186% similar) in 86 aa overlap (1-82:137-222) 10 20 30 FLJ001 SAELEVQRRHSLVSFVVRIVPSPDWFVGVD :::::::::::::::::::::::::::::: gi|108 MKEIEAAGEALQSVHAVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVD 110 120 130 140 150 160 40 50 60 70 80 FLJ001 SLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEVSG----HPACARA ::::::::: :::::::::::.::::::::::::::::::::::::... ::: gi|108 SLDLCDGDRGREQAALDLYPYEAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFY 170 180 190 200 210 220 90 100 110 120 130 140 FLJ001 AMAPAVGTGLGPGCTLASQPRTAACGFCVEEALLLAPAVSKGVQPSHTLPTRGHSTAGRT gi|108 YPRLKALPPIARVTLVRLRQSPRAFVPSAPVLPSRDNEIIDSASVPETPLDCEVSLWSSW 230 240 250 260 270 280 216 residues in 1 query sequences 2693465022 residues in 7827732 library sequences Tcomplib [34.26] (2 proc) start: Fri Feb 27 23:35:05 2009 done: Fri Feb 27 23:39:57 2009 Total Scan time: 691.910 Total Display time: 0.020 Function used was FASTA [version 34.26.5 April 26, 2007]