##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI AlwNI AocI AscI
Asp718I AspEI AspI BamHI BbeI
BbrPI BcgI BglI BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BssHII Bst1107I BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
FseI HgaI Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NarI NheI
NotI NruI PacI PflFI PinAI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AclI 3199 AACGTT AA^CGTT
Aor51HI 4128 AGCGCT AGC^GCT
ApaI 3961 GGGCCC GGGCC^C
AseI 4415 ATTAAT AT^TAAT
AsnI 4415 ATTAAT AT^TAAT
Asp700I 3648 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 957 CTCGAG C^[CT]CG[GA]G
AvrII 2625 CCTAGG C^CTAGG
BbvCI 156 CCTCAGC CCTCAGC(-5/-2)
BciVI 4320 GGATAC GTATCC(6/5)
BlnI 2625 CCTAGG C^CTAGG
BlpI 2536 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2536 GCTGAGC GC^T[ACGT]AGC
BsoBI 957 CTCGAG C^[CT]CG[GA]G
BsrFI 3049 GCCGGC [GA]^CCGG[CT]
CelII 2536 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3049 GCCGGC [GA]^CCGG[CT]
Eco47III 4128 AGCGCT AGC^GCT
EcoNI 1944 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3019 GATATC GAT^ATC
HindIII 4331 AAGCTT A^AGCTT
NaeI 3049 GCCGGC GCC^GGC
NgoMIV 3049 GCCGGC G^CCGGC
PaeR7I 957 CTCGAG C^TCGAG
PflMI 1408 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1159 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4415 ATTAAT AT^TAAT
Psp1406I 3199 AACGTT AA^CGTT
SphI 2843 GCATGC GCATG^C
SspI 1476 AATATT AAT^ATT
Van91I 1408 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 957 CTCGAG C^TCGAG
XmnI 3648 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Alw44I 1643 GTGCAC G^TGCAC
2028 GTGCAC
ApaLI 1643 GTGCAC G^TGCAC
2028 GTGCAC
AviII 2968 TGCGCA TGC^GCA
3871 TGCGCA
BalI 25 TGGCCA TGG^CCA
1598 TGGCCA
BanI 2146 GGCACC G^G[CT][GA]CC
3529 GGCACC
BclI 327 TGATCA T^GATCA
1832 TGATCA
BglII 3003 AGATCT A^GATCT
3620 AGATCT
BseRI 643 GAGGAG GAGGAG(10/8)
2770 GAGGAG
BspHI 438 TCATGA T^CATGA
656 TCATGA
BssSI 628 CACGAG CACGAG(-5/-1)
3259 CTCGTG
BstBI 2272 TTCGAA TT^CGAA
3514 TTCGAA
DrdI 351 GACTCACACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1963 GACTCAAAAGTC
EaeI 25 TGGCCA [CT]^GGCC[GA]
1598 TGGCCA
EcoT22I 1301 ATGCAT ATGCA^T
3357 ATGCAT
FbaI 327 TGATCA T^GATCA
1832 TGATCA
FspI 2968 TGCGCA TGC^GCA
3871 TGCGCA
HaeII 3073 AGCGCC [GA]GCGC^[CT]
4128 AGCGCT
HincII 1225 GTCAAC GT[CT]^[GA]AC
2404 GTTGAC
HindII 1225 GTCAAC GT[CT]^[GA]AC
2404 GTTGAC
MluNI 25 TGGCCA TGG^CCA
1598 TGGCCA
MscI 25 TGGCCA TGG^CCA
1598 TGGCCA
MspA1I 984 CAGCTG C[AC]G^C[GT]G
4209 CAGCTG
NdeI 2611 CATATG CA^TATG
3035 CATATG
NsiI 1301 ATGCAT ATGCA^T
3357 ATGCAT
NspV 2272 TTCGAA TT^CGAA
3514 TTCGAA
PpuMI 1100 AGGTCCT [GA]G^G[AT]CC[CT]
1157 AGGACCC
PvuII 984 CAGCTG CAG^CTG
4209 CAGCTG
RcaI 438 TCATGA T^CATGA
656 TCATGA
SapI 1354 GAAGAGC GCTCTTC(1/4)
633 GCTCTTC
SfuI 2272 TTCGAA TT^CGAA
3514 TTCGAA
XcmI 851 CCAACCTCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2996 CCAAGCAAGATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI ApoI AspHI
AvaII BanII BbsI BbvI BcnI
BfaI BfrI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspLU11I BspMI BsrBI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII