##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccIII AgeI AhdI Alw44I AocI
ApaLI AscI AseI AsnI AspEI
BbrPI BcgI BsaAI BsaBI BseAI
BsiWI BspDI BspEI BspHI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI Eam1105I Eco81I EcoRI
EcoRV FseI HindIII MamI MluI
MroI NdeI NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RcaI
RsrII SalI ScaI SexAI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 274 GGTACC G^GTACC
AccI 702 GTCTAC GT^[AC][GT]AC
AclI 4171 AACGTT AA^CGTT
AflIII 4550 ACATGT A^C[GA][CT]GT
Aor51HI 3587 AGCGCT AGC^GCT
Asp718I 274 GGTACC G^GTACC
AspI 957 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 457 TGCGCA TGC^GCA
BamHI 102 GGATCC G^GATCC
BbeI 3534 GGCGCC GGCGC^C
BciVI 817 GGATAC GTATCC(6/5)
BclI 4413 TGATCA T^GATCA
BglII 1568 AGATCT A^GATCT
BsaI 1232 GAGACC GGTCTC(1/5)
BsiEI 2 CGGCCG CG[GA][CT]^CG
BsmBI 934 GAGACG CGTCTC(1/5)
Bsp1407I 2192 TGTACA T^GTACA
BspLU11I 4550 ACATGT A^CATGT
BsrGI 2192 TGTACA T^GTACA
BstEII 2732 GGTCACC G^GT[ACGT]ACC
BstPI 2732 GGTCACC G^GT[ACGT]ACC
DraIII 249 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 57 GACATGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2 CGGCCG C^GGCCG
EclXI 2 CGGCCG C^GGCCG
Eco47III 3587 AGCGCT AGC^GCT
Eco52I 2 CGGCCG C^GGCCG
EcoO65I 2732 GGTCACC G^GT[ACGT]ACC
EcoT22I 4491 ATGCAT ATGCA^T
FbaI 4413 TGATCA T^GATCA
FspI 457 TGCGCA TGC^GCA
HpaI 4168 GTTAAC GTT^AAC
KasI 3534 GGCGCC G^GCGCC
KpnI 274 GGTACC GGTAC^C
KspI 3210 CCGCGG CCGC^GG
MfeI 2417 CAATTG C^AATTG
MunI 2417 CAATTG C^AATTG
NarI 3534 GGCGCC GG^CGCC
NsiI 4491 ATGCAT ATGCA^T
PflFI 957 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 4171 AACGTT AA^CGTT
SacII 3210 CCGCGG CCGC^GG
SbfI 217 CCTGCAGG CCTGCA^GG
SfoI 3534 GGCGCC GGC^GCC
SmaI 3456 CCCGGG CCC^GGG
Sse8387I 217 CCTGCAGG CCTGCA^GG
SspBI 2192 TGTACA T^GTACA
Tth111I 957 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3456 CCCGGG C^CCGGG
XmaI 3456 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 936 GACGTC GACGT^C
3397 GACGTC
AflII 1275 CTTAAG C^TTAAG
2840 CTTAAG
ApaI 230 GGGCCC GGGCC^C
2288 GGGCCC
Asp700I 2404 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3642 GAACTAATTC
AvrII 137 CCTAGG C^CTAGG
2696 CCTAGG
BbsI 939 GTCTTC GAAGAC(2/6)
2032 GTCTTC
BfrI 1275 CTTAAG C^TTAAG
2840 CTTAAG
BlnI 137 CCTAGG C^CTAGG
2696 CCTAGG
BpuAI 939 GTCTTC GAAGAC(2/6)
2032 GTCTTC
BsaWI 143 TCCGGT [AT]^CCGG[AT]
3055 TCCGGT
BsmI 1611 GCATTC GAATGC(1/-1)
4323 GCATTC
BsrBI 16 CCGCTC CCGCTC(-3/-3)
3808 CCGCTC
BsrDI 1840 CATTGC GCAATG(2/0)
4338 CATTGC
BsrFI 84 GCCGGC [GA]^CCGG[CT]
316 GCCGGC
BstAPI 3079 GCATGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4323 GCATTCCCTGC
BstYI 102 GGATCC [GA]^GATC[CT]
1568 AGATCT
Cfr10I 84 GCCGGC [GA]^CCGG[CT]
316 GCCGGC
EcoNI 4008 CCTTTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4131 CCTTCCCCAGG
HincII 1574 GTCAAC GT[CT]^[GA]AC
4168 GTTAAC
HindII 1574 GTCAAC GT[CT]^[GA]AC
4168 GTTAAC
MflI 102 GGATCC [GA]^GATC[CT]
1568 AGATCT
NaeI 84 GCCGGC GCC^GGC
316 GCCGGC
NgoMIV 84 GCCGGC G^CCGGC
316 GCCGGC
PaeR7I 284 CTCGAG C^TCGAG
2652 CTCGAG
SacI 3341 GAGCTC GAGCT^C
3960 GAGCTC
SfiI 74 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1071 GGCCTCCTTGGCC
XhoI 284 CTCGAG C^TCGAG
2652 CTCGAG
XhoII 102 GGATCC [GA]^GATC[CT]
1568 AGATCT
XmnI 2404 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3642 GAACTAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI