##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BbvCI BcgI
BfrI BglII BlnI BsaBI BseAI
BsiEI BsiWI BsmBI BspEI BspHI
BspLU11I BssHII Bst1107I BstBI BstZ17I
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV EcoT22I
FseI HincII HindII HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1173 GTCTAC GT^[AC][GT]AC
AflIII 2340 ACGTGT A^C[GA][CT]GT
Aor51HI 3218 AGCGCT AGC^GCT
AviII 4125 TGCGCA TGC^GCA
BclI 15 TGATCA T^GATCA
BlpI 1271 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1271 GCTGAGC GC^T[ACGT]AGC
BsaWI 51 ACCGGA [AT]^CCGG[AT]
BsmI 3508 GAATGC GAATGC(1/-1)
Bsp1407I 1036 TGTACA T^GTACA
BspDI 3941 ATCGAT AT^CGAT
BsrBI 3374 GAGCGG CCGCTC(-3/-3)
BsrGI 1036 TGTACA T^GTACA
BssSI 1165 CTCGTG CACGAG(-5/-1)
BstAPI 4153 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 300 GGTAACC G^GT[ACGT]ACC
BstPI 300 GGTAACC G^GT[ACGT]ACC
BstXI 3660 CCAGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1271 GCTGAGC GC^T[ACGT]AGC
ClaI 3941 ATCGAT AT^CGAT
Eco47III 3218 AGCGCT AGC^GCT
EcoO65I 300 GGTAACC G^GT[ACGT]ACC
FbaI 15 TGATCA T^GATCA
FspI 4125 TGCGCA TGC^GCA
HindIII 2349 AAGCTT A^AGCTT
NcoI 3828 CCATGG C^CATGG
SfiI 3082 GGCCACAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1267 GCATGC GCATG^C
SspBI 1036 TGTACA T^GTACA
SspI 4528 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AocI 762 CCTCAGG CC^T[ACGT]AGG
4383 CCTGAGG
BbrPI 2339 CACGTG CAC^GTG
2966 CACGTG
BsaAI 2339 CACGTG [CT]AC^GT[GA]
2966 CACGTG
BsrFI 3440 ACCGGC [GA]^CCGG[CT]
3492 ACCGGC
Bsu36I 762 CCTCAGG CC^T[ACGT]AGG
4383 CCTGAGG
Cfr10I 3440 ACCGGC [GA]^CCGG[CT]
3492 ACCGGC
DraI 498 TTTAAA TTT^AAA
1697 TTTAAA
Eco81I 762 CCTCAGG CC^T[ACGT]AGG
4383 CCTGAGG
HgaI 1329 GACGC GACGC(5/10)
3369 GCGTC
PaeR7I 1945 CTCGAG C^TCGAG
2004 CTCGAG
PmaCI 2339 CACGTG CAC^GTG
2966 CACGTG
PmlI 2339 CACGTG CAC^GTG
2966 CACGTG
XhoI 1945 CTCGAG C^TCGAG
2004 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbeI BbsI BbvI BciVI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshAI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII