##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AspI AvaI AvaII
AviII BamHI BanII BbeI BbrPI
BclI BfrI BglI BglII BlpI
Bpu1102I BsaHI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsoBI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstEII BstPI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRV FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MflI
MluI MroI MspA1I NaeI NarI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 290 GTCTAC GT^[AC][GT]AC
AlwNI 2418 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AvrII 2404 CCTAGG C^CTAGG
BcgI 2513 CGAAAAGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 3253 CCGGG CC^[GC]GG
BlnI 2404 CCTAGG C^CTAGG
BsaAI 3755 TACGTG [CT]AC^GT[GA]
BsaBI 2323 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 2972 GAGGAG GAGGAG(10/8)
BsgI 3358 CTGCAC GTGCAG(16/14)
Bsp1407I 1950 TGTACA T^GTACA
BsrGI 1950 TGTACA T^GTACA
BstBI 470 TTCGAA TT^CGAA
CfoI 3123 GCGC GCG^C
Cfr13I 134 GGCCC G^G[ACGT]CC
DraI 2771 TTTAAA TTT^AAA
DsaI 2657 CCATGG C^C[GA][CT]GG
HhaI 3123 GCGC GCG^C
HinP1I 3123 GCGC G^CGC
MaeII 3756 ACGT A^CGT
MamI 2323 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3227 CAATTG C^AATTG
MunI 3227 CAATTG C^AATTG
NciI 3253 CCGGG CC^[GC]GG
NcoI 2657 CCATGG C^CATGG
NspI 4128 GCATGC [GA]CATG^[CT]
NspV 470 TTCGAA TT^CGAA
SapI 791 GCTCTTC GCTCTTC(1/4)
Sau96I 134 GGCCC G^G[ACGT]CC
SfuI 470 TTCGAA TT^CGAA
SmlI 4175 CTTGAG C^T[CT][GA]AG
SphI 4128 GCATGC GCATG^C
SspBI 1950 TGTACA T^GTACA
StuI 2297 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 162 CGCG CG^CG
3124 CGCG
AseI 2231 ATTAAT AT^TAAT
4200 ATTAAT
AsnI 2231 ATTAAT AT^TAAT
4200 ATTAAT
BalI 2206 TGGCCA TGG^CCA
4068 TGGCCA
BbvI 239 GCAGC GCAGC(8/12)
1120 GCAGC
BciVI 3633 GGATAC GTATCC(6/5)
3778 GGATAC
BmyI 3758 GTGCCC G[AGT]GC[ACT]^C
4233 GGGCAC
BsaWI 1761 ACCGGA [AT]^CCGG[AT]
2097 ACCGGA
Bsp1286I 3758 GTGCCC G[AGT]GC[ACT]^C
4233 GGGCAC
BstUI 162 CGCG CG^CG
3124 CGCG
DraIII 3 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
3764 CACACTGTG
EaeI 2206 TGGCCA [CT]^GGCC[GA]
4068 TGGCCA
HindIII 186 AAGCTT A^AGCTT
212 AAGCTT
MluNI 2206 TGGCCA TGG^CCA
4068 TGGCCA
MscI 2206 TGGCCA TGG^CCA
4068 TGGCCA
MvnI 162 CGCG CG^CG
3124 CGCG
PflMI 2 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3763 CCACACTGTGG
PleI 1754 GACTC GAGTC(4/5)
4178 GAGTC
PshBI 2231 ATTAAT AT^TAAT
4200 ATTAAT
PstI 237 CTGCAG CTGCA^G
3017 CTGCAG
ScaI 2884 AGTACT AGT^ACT
4014 AGTACT
TseI 239 GCAGC G^C[AT]GC
1120 GCAGC
Van91I 2 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3763 CCACACTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI Asp700I BanI BbsI
BbvCI BfaI BpmI BpuAI BsaI
BsaJI BsiYI BslI BsmAI BsmI
BspHI BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI Bsu36I
Cac8I DdeI DpnII EarI Eco81I
EcoRI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NdeI NdeII
NlaIII NlaIV NsiI RcaI RsaI
Sau3AI ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI TthHB8I XmnI