##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp700I
AspEI AviII AvrII BbeI BcgI
BciVI BfrI BglII BlnI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspHI
BsrGI BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FseI FspI HindIII KasI
KspI MfeI MluI MunI NarI
NdeI NheI NotI NsiI NspV
PacI PinAI PmeI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SpeI Sse8387I SspBI SspI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 3647 GGTACC G^GTACC
AccI 3072 GTCTAC GT^[AC][GT]AC
AseI 4671 ATTAAT AT^TAAT
AsnI 4671 ATTAAT AT^TAAT
Asp718I 3647 GGTACC G^GTACC
BbvCI 2432 GCTGAGG CCTCAGC(-5/-2)
BclI 3244 TGATCA T^GATCA
BsaBI 2702 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 2188 CATTGC GCAATG(2/0)
BssHII 1196 GCGCGC G^CGCGC
BstAPI 2208 GCATTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3452 GGTCACC G^GT[ACGT]ACC
BstPI 3452 GGTCACC G^GT[ACGT]ACC
DraIII 412 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 3452 GGTCACC G^GT[ACGT]ACC
FbaI 3244 TGATCA T^GATCA
HpaI 4340 GTTAAC GTT^AAC
KpnI 3647 GGTACC GGTAC^C
MamI 2702 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NruI 1111 TCGCGA TCG^CGA
PshAI 149 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4671 ATTAAT AT^TAAT
SnaBI 118 TACGTA TAC^GTA
SphI 1189 GCATGC GCATG^C
XbaI 3985 TCTAGA T^CTAGA
XcmI 956 CCAGCAGCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 2625 TCCGGA T^CCGGA
3868 TCCGGA
AcyI 2764 GGCGTC G[GA]^CG[CT]C
4093 GACGCC
AocI 329 CCTGAGG CC^T[ACGT]AGG
1895 CCTGAGG
BalI 1362 TGGCCA TGG^CCA
3064 TGGCCA
BbrPI 2945 CACGTG CAC^GTG
4349 CACGTG
BbsI 49 GAAGAC GAAGAC(2/6)
4081 GTCTTC
BpuAI 49 GAAGAC GAAGAC(2/6)
4081 GTCTTC
BsaHI 2764 GGCGTC G[GA]^CG[CT]C
4093 GACGCC
BseAI 2625 TCCGGA T^CCGGA
3868 TCCGGA
BspEI 2625 TCCGGA T^CCGGA
3868 TCCGGA
BspMI 1491 ACCTGC ACCTGC(4/8)
754 GCAGGT
Bsu36I 329 CCTGAGG CC^T[ACGT]AGG
1895 CCTGAGG
Eco81I 329 CCTGAGG CC^T[ACGT]AGG
1895 CCTGAGG
Hin1I 2764 GGCGTC G[GA]^CG[CT]C
4093 GACGCC
HincII 3540 GTCAAC GT[CT]^[GA]AC
4340 GTTAAC
HindII 3540 GTCAAC GT[CT]^[GA]AC
4340 GTTAAC
MluNI 1362 TGGCCA TGG^CCA
3064 TGGCCA
MroI 2625 TCCGGA T^CCGGA
3868 TCCGGA
MscI 1362 TGGCCA TGG^CCA
3064 TGGCCA
NaeI 2859 GCCGGC GCC^GGC
3223 GCCGGC
NcoI 450 CCATGG C^CATGG
4026 CCATGG
NgoMIV 2859 GCCGGC G^CCGGC
3223 GCCGGC
PaeR7I 274 CTCGAG C^TCGAG
1683 CTCGAG
PmaCI 2945 CACGTG CAC^GTG
4349 CACGTG
PmlI 2945 CACGTG CAC^GTG
4349 CACGTG
SapI 3925 GAAGAGC GCTCTTC(1/4)
1333 GCTCTTC
StuI 1984 AGGCCT AGG^CCT
4576 AGGCCT
XhoI 274 CTCGAG C^TCGAG
1683 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AspHI AspI AvaI AvaII BamHI
BanI BanII BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspLU11I
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XhoII
XmaCI XmaI