##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AflII AgeI AscI AviII BbeI
BcgI BfrI BglII BsaBI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstZ17I
Cfr10I ClaI CpoI DraI DraIII
EcoRI FseI FspI HindIII KasI
MamI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NotI PacI
PinAI PmeI PvuI RcaI RsrII
SalI SfiI SfoI SgrAI SnaBI
SpeI SspBI SwaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 315 GACGTC GACGT^C
Acc65I 375 GGTACC G^GTACC
AclI 3404 AACGTT AA^CGTT
AcsI 3887 AAATTC [GA]^AATT[CT]
AhdI 3683 GACGAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 673 AGCGCT AGC^GCT
ApaI 1957 GGGCCC GGGCC^C
ApoI 3887 AAATTC [GA]^AATT[CT]
AseI 2225 ATTAAT AT^TAAT
AsnI 2225 ATTAAT AT^TAAT
Asp718I 375 GGTACC G^GTACC
AspEI 3683 GACGAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1875 CACGTG CAC^GTG
BbsI 573 GTCTTC GAAGAC(2/6)
BbvCI 4359 CCTCAGC CCTCAGC(-5/-2)
BciVI 277 GTATCC GTATCC(6/5)
BclI 2389 TGATCA T^GATCA
BglI 1661 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 573 GTCTTC GAAGAC(2/6)
BsmBI 84 GAGACG CGTCTC(1/5)
BsmI 2927 GAATGC GAATGC(1/-1)
BstAPI 296 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2109 TTCGAA TT^CGAA
BstEII 2658 GGTCACC G^GT[ACGT]ACC
BstPI 2658 GGTCACC G^GT[ACGT]ACC
DrdI 2953 GACTTCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3683 GACGAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 673 AGCGCT AGC^GCT
EcoO65I 2658 GGTCACC G^GT[ACGT]ACC
EcoRV 3075 GATATC GAT^ATC
EcoT22I 1076 ATGCAT ATGCA^T
FbaI 2389 TGATCA T^GATCA
KpnI 375 GGTACC GGTAC^C
NheI 3331 GCTAGC G^CTAGC
NruI 3986 TCGCGA TCG^CGA
NsiI 1076 ATGCAT ATGCA^T
NspV 2109 TTCGAA TT^CGAA
PflMI 2075 CCAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1875 CACGTG CAC^GTG
PmlI 1875 CACGTG CAC^GTG
PshBI 2225 ATTAAT AT^TAAT
Psp1406I 3404 AACGTT AA^CGTT
SbfI 407 CCTGCAGG CCTGCA^GG
ScaI 1984 AGTACT AGT^ACT
SexAI 2588 ACCTGGT A^CC[AT]GGT
SfuI 2109 TTCGAA TT^CGAA
SphI 1028 GCATGC GCATG^C
Sse8387I 407 CCTGCAGG CCTGCA^GG
SspI 2235 AATATT AAT^ATT
Tsp509I 3888 AATT ^AATT
Van91I 2075 CCAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3345 CCAGCTTCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1753 GTCTAC GT^[AC][GT]AC
4335 GTCTAC
AccIII 1619 TCCGGA T^CCGGA
3395 TCCGGA
AflIII 598 ACGTGT A^C[GA][CT]GT
982 ACACGT
Asp700I 852 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3839 GAAACACTTC
AspI 2587 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4125 GACAAGGTC
BamHI 395 GGATCC G^GATCC
3962 GGATCC
BseAI 1619 TCCGGA T^CCGGA
3395 TCCGGA
BsiEI 1588 CGGCCG CG[GA][CT]^CG
1853 CGGCCG
BspEI 1619 TCCGGA T^CCGGA
3395 TCCGGA
BstXI 1264 CCAGCGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3506 CCAGACAGATGG
EagI 1588 CGGCCG C^GGCCG
1853 CGGCCG
EclXI 1588 CGGCCG C^GGCCG
1853 CGGCCG
Eco52I 1588 CGGCCG C^GGCCG
1853 CGGCCG
HincII 2188 GTTAAC GT[CT]^[GA]AC
3579 GTTAAC
HindII 2188 GTTAAC GT[CT]^[GA]AC
3579 GTTAAC
HpaI 2188 GTTAAC GTT^AAC
3579 GTTAAC
KspI 64 CCGCGG CCGC^GG
692 CCGCGG
MroI 1619 TCCGGA T^CCGGA
3395 TCCGGA
NcoI 3659 CCATGG C^CATGG
3694 CCATGG
NspI 1028 GCATGC [GA]CATG^[CT]
1074 ACATGC
PaeR7I 2757 CTCGAG C^TCGAG
3731 CTCGAG
PflFI 2587 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4125 GACAAGGTC
PshAI 979 GACACACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4077 GACTCCTGTC
SacII 64 CCGCGG CCGC^GG
692 CCGCGG
SapI 2347 GAAGAGC GCTCTTC(1/4)
3059 GCTCTTC
Tth111I 2587 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4125 GACAAGGTC
XbaI 2251 TCTAGA T^CTAGA
2768 TCTAGA
XhoI 2757 CTCGAG C^TCGAG
3731 CTCGAG
XmnI 852 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3839 GAAACACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaLI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbvI BcnI BfaI
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I XhoII XmaCI XmaI