##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbeI BciVI BfrI
BglII BlnI BsaBI BseAI BsiEI
BsiWI BspDI BspEI BspLU11I BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoT22I FseI FspI HpaI KasI
KpnI MamI MfeI MluI MroI
MunI NarI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SfoI SfuI
SgrAI SnaBI SpeI SwaI Tth111I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 840 CGCG CG^CG
AcsI 1806 GAATTT [GA]^AATT[CT]
AcyI 341 GGCGTC G[GA]^CG[CT]C
ApoI 1806 GAATTT [GA]^AATT[CT]
BamHI 2078 GGATCC G^GATCC
BbrPI 1455 CACGTG CAC^GTG
BbsI 1827 GTCTTC GAAGAC(2/6)
BcgI 3062 GCAGTTTACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1827 GTCTTC GAAGAC(2/6)
BsaAI 1455 CACGTG [CT]AC^GT[GA]
BsaHI 341 GGCGTC G[GA]^CG[CT]C
BsmBI 1386 GAGACG CGTCTC(1/5)
BsmI 1677 GCATTC GAATGC(1/-1)
Bsp1407I 1696 TGTACA T^GTACA
BspHI 2336 TCATGA T^CATGA
BsrGI 1696 TGTACA T^GTACA
BstUI 840 CGCG CG^CG
BstXI 1061 CCAGAACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1545 GACGAATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1600 GATATC GAT^ATC
Hin1I 341 GGCGTC G[GA]^CG[CT]C
HincII 3213 GTCAAC GT[CT]^[GA]AC
HindII 3213 GTCAAC GT[CT]^[GA]AC
HindIII 78 AAGCTT A^AGCTT
KspI 839 CCGCGG CCGC^GG
MslI 3634 CATCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 840 CGCG CG^CG
NdeI 2696 CATATG CA^TATG
PmaCI 1455 CACGTG CAC^GTG
PmlI 1455 CACGTG CAC^GTG
RcaI 2336 TCATGA T^CATGA
SacII 839 CCGCGG CCGC^GG
SapI 1848 GAAGAGC GCTCTTC(1/4)
SbfI 4117 CCTGCAGG CCTGCA^GG
ScaI 631 AGTACT AGT^ACT
SexAI 3309 ACCTGGT A^CC[AT]GGT
SfiI 1427 GGCCACGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1765 CCCGGG CCC^GGG
Sse8387I 4117 CCTGCAGG CCTGCA^GG
SspBI 1696 TGTACA T^GTACA
SspI 4290 AATATT AAT^ATT
XbaI 2263 TCTAGA T^CTAGA
XmaCI 1765 CCCGGG C^CCGGG
XmaI 1765 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 500 ACGTGT A^C[GA][CT]GT
1454 ACACGT
Alw44I 3436 GTGCAC G^TGCAC
4085 GTGCAC
ApaLI 3436 GTGCAC G^TGCAC
4085 GTGCAC
AvaI 1765 CCCGGG C^[CT]CG[GA]G
3070 CTCGGG
BalI 1426 TGGCCA TGG^CCA
2084 TGGCCA
BsoBI 1765 CCCGGG C^[CT]CG[GA]G
3070 CTCGGG
BsrDI 658 GCAATG GCAATG(2/0)
4055 GCAATG
BsrFI 62 GCCGGC [GA]^CCGG[CT]
2063 GCCGGC
BssSI 1430 CACGAG CACGAG(-5/-1)
3439 CACGAG
Cfr10I 62 GCCGGC [GA]^CCGG[CT]
2063 GCCGGC
DraIII 3825 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
4088 CACTGTGTG
EaeI 1426 TGGCCA [CT]^GGCC[GA]
2084 TGGCCA
MluNI 1426 TGGCCA TGG^CCA
2084 TGGCCA
MscI 1426 TGGCCA TGG^CCA
2084 TGGCCA
NaeI 62 GCCGGC GCC^GGC
2063 GCCGGC
NgoMIV 62 GCCGGC G^CCGGC
2063 GCCGGC
NspI 830 GCATGC [GA]CATG^[CT]
4217 GCATGC
SacI 1395 GAGCTC GAGCT^C
3851 GAGCTC
SphI 830 GCATGC GCATG^C
4217 GCATGC
TaqI 700 TCGA T^CGA
2030 TCGA
TfiI 2207 GAATC G^A[AT]TC
2507 GAATC
Tsp509I 1807 AATT ^AATT
4245 AATT
TthHB8I 700 TCGA T^CGA
2030 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI Aor51HI ApaI AspHI AvaII
BanI BanII BbvCI BbvI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI Tru9I TseI Tsp45I
TspRI Van91I XcmI XhoII