##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AclI AflII AscI AseI
AsnI AspI AvrII BbvCI BcgI
BfrI BglII BlnI BsaBI BsiWI
BsmBI BspHI BspLU11I BssHII BstBI
BstEII BstPI CpoI DraI DraIII
DrdI EcoO65I EcoRI EcoT22I FseI
HpaI KspI MamI MfeI MluI
MunI NdeI NotI NruI NsiI
NspV PacI PflFI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1514 GGTACC G^GTACC
AccIII 156 TCCGGA T^CCGGA
AflIII 2827 ACGTGT A^C[GA][CT]GT
AgeI 1754 ACCGGT A^CCGGT
AhdI 1699 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 127 GTGCAC G^TGCAC
Aor51HI 3705 AGCGCT AGC^GCT
ApaLI 127 GTGCAC G^TGCAC
Asp700I 219 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1514 GGTACC G^GTACC
AspEI 1699 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4612 TGCGCA TGC^GCA
BciVI 2341 GTATCC GTATCC(6/5)
BclI 1885 TGATCA T^GATCA
BseAI 156 TCCGGA T^CCGGA
BsiEI 68 CGGCCG CG[GA][CT]^CG
Bsp1407I 2296 TGTACA T^GTACA
BspDI 4428 ATCGAT AT^CGAT
BspEI 156 TCCGGA T^CCGGA
BsrGI 2296 TGTACA T^GTACA
Bst1107I 1734 GTATAC GTA^TAC
BstAPI 4640 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 4147 CCAGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1734 GTATAC GTA^TAC
ClaI 4428 ATCGAT AT^CGAT
EagI 68 CGGCCG C^GGCCG
Eam1105I 1699 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 68 CGGCCG C^GGCCG
Eco47III 3705 AGCGCT AGC^GCT
Eco52I 68 CGGCCG C^GGCCG
EcoRV 113 GATATC GAT^ATC
FbaI 1885 TGATCA T^GATCA
FspI 4612 TGCGCA TGC^GCA
HincII 1521 GTCAAC GT[CT]^[GA]AC
HindII 1521 GTCAAC GT[CT]^[GA]AC
KpnI 1514 GGTACC GGTAC^C
MroI 156 TCCGGA T^CCGGA
NaeI 11 GCCGGC GCC^GGC
NgoMIV 11 GCCGGC G^CCGGC
NheI 1202 GCTAGC G^CTAGC
PaeR7I 1684 CTCGAG C^TCGAG
PinAI 1754 ACCGGT A^CCGGT
PshAI 2692 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 77 AGTACT AGT^ACT
SmaI 604 CCCGGG CCC^GGG
SphI 2527 GCATGC GCATG^C
SspBI 2296 TGTACA T^GTACA
SspI 5015 AATATT AAT^ATT
XhoI 1684 CTCGAG C^TCGAG
XmaCI 604 CCCGGG C^CCGGG
XmaI 604 CCCGGG C^CCGGG
XmnI 219 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 1734 GTATAC GT^[AC][GT]AC
2433 GTCTAC
AcsI 3146 AAATTC [GA]^AATT[CT]
4981 AAATTC
ApoI 3146 AAATTC [GA]^AATT[CT]
4981 AAATTC
BbrPI 2826 CACGTG CAC^GTG
3453 CACGTG
BlpI 314 GCTCAGC GC^T[ACGT]AGC
2531 GCTGAGC
Bpu1102I 314 GCTCAGC GC^T[ACGT]AGC
2531 GCTGAGC
BsaAI 2826 CACGTG [CT]AC^GT[GA]
3453 CACGTG
BsrBI 261 CCGCTC CCGCTC(-3/-3)
3861 GAGCGG
CelII 314 GCTCAGC GC^T[ACGT]AGC
2531 GCTGAGC
HindIII 1593 AAGCTT A^AGCTT
2836 AAGCTT
NspI 2180 GCATGT [GA]CATG^[CT]
2527 GCATGC
PmaCI 2826 CACGTG CAC^GTG
3453 CACGTG
PmlI 2826 CACGTG CAC^GTG
3453 CACGTG
SfiI 914 GGCCCGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3569 GGCCACAGGGGCC
SmlI 1684 CTCGAG C^T[CT][GA]AG
3615 CTTGAG
Tsp509I 3147 AATT ^AATT
4982 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII