BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149547.9 + phase: 0
(180 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
P4HA_CHICK (P16924) Prolyl 4-hydroxylase alpha subunit (EC 1.14.... 114 1e-25
P4H1_RAT (P54001) Prolyl 4-hydroxylase alpha-1 subunit precursor... 114 2e-25
P4H1_MOUSE (Q60715) Prolyl 4-hydroxylase alpha-1 subunit precurs... 106 3e-23
P4H1_HUMAN (P13674) Prolyl 4-hydroxylase alpha-1 subunit precurs... 105 6e-23
P4H2_HUMAN (O15460) Prolyl 4-hydroxylase alpha-2 subunit precurs... 105 7e-23
P4H2_MOUSE (Q60716) Prolyl 4-hydroxylase alpha-2 subunit precurs... 102 5e-22
P4H2_CAEEL (Q20065) Prolyl 4-hydroxylase alpha-2 subunit precurs... 99 4e-21
P4H1_CAEEL (Q10576) Prolyl 4-hydroxylase alpha-1 subunit precurs... 95 8e-20
EGLX_MOUSE (Q8BG58) Putative HIF-prolyl hydroxylase PH-4 (EC 1.1... 73 4e-13
EGLX_HUMAN (Q9NXG6) Putative HIF-prolyl hydroxylase PH-4 (EC 1.1... 72 5e-13
XA5L_MOUSE (Q9WTJ8) XAP-5-like protein 29 5.2
V006_FOWPV (P14361) Hypothetical protein FPV006/FPV255 (BamHI-ORF1) 29 5.2
SYIC_SCHPO (O13651) Isoleucyl-tRNA synthetase, cytoplasmic (EC 6... 29 5.2
SFL1_YEAST (P20134) Flocculation suppression protein (SFL1 protein) 29 6.7
ER1A_HUMAN (Q96HE7) ERO1-like protein alpha precursor (EC 1.8.4.... 28 8.8
>P4HA_CHICK (P16924) Prolyl 4-hydroxylase alpha subunit (EC
1.14.11.2) (4-PH alpha)
(Procollagen-proline,2-oxoglutarate-4-dioxygenase alpha
subunit)
Length = 516
Score = 114 bits (285), Expect = 1e-25
Identities = 64/178 (35%), Positives = 87/178 (47%), Gaps = 23/178 (12%)
Query: 1 MHKSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVEHGENFNVLH 60
+ ++ V D ETG + R S A+L +V I RI D T + V E V +
Sbjct: 342 LSRATVHDPETGKLTTAHYRVSKSAWLSGYESPVVSRINTRIQDLTGLDVSTAEELQVAN 401
Query: 61 YEVGQKYEPHYDYFM----DTFSTTYAGQRIATMLMYLSDVEEGGETVFPNAKGNFSSVP 116
Y VG +YEPH+D+ D F G RIAT L Y+SDV GG TVFP
Sbjct: 402 YGVGGQYEPHFDFGRKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEV-------- 453
Query: 117 WWNELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMH 174
G S+ PK G A+ ++++ P D S+ H ACPV+ G+KW+ KW+H
Sbjct: 454 -----------GASVWPKKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKWVSNKWLH 500
>P4H1_RAT (P54001) Prolyl 4-hydroxylase alpha-1 subunit precursor
(EC 1.14.11.2) (4-PH alpha-1)
(Procollagen-proline,2-oxoglutarate-4-dioxygenase
alpha-1 subunit)
Length = 534
Score = 114 bits (284), Expect = 2e-25
Identities = 64/178 (35%), Positives = 88/178 (48%), Gaps = 23/178 (12%)
Query: 1 MHKSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVEHGENFNVLH 60
+ ++ V D ETG ++ R S A+L D +V I RI D T + V E V +
Sbjct: 360 LSRATVHDPETGKLTTAQYRVSKSAWLSGYEDPVVSRINMRIQDLTGLDVSTAEELQVAN 419
Query: 61 YEVGQKYEPHYDYFM----DTFSTTYAGQRIATMLMYLSDVEEGGETVFPNAKGNFSSVP 116
Y VG +YEPH+D+ D F G RIAT L Y+SDV GG TVFP
Sbjct: 420 YGVGGQYEPHFDFARKDEPDAFRELGTGNRIATWLFYMSDVSAGGATVFPEV-------- 471
Query: 117 WWNELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMH 174
G S+ PK G A+ ++++ D S+ H ACPV+ G+KW+ KW+H
Sbjct: 472 -----------GASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLH 518
>P4H1_MOUSE (Q60715) Prolyl 4-hydroxylase alpha-1 subunit precursor
(EC 1.14.11.2) (4-PH alpha-1)
(Procollagen-proline,2-oxoglutarate-4-dioxygenase
alpha-1 subunit)
Length = 534
Score = 106 bits (264), Expect = 3e-23
Identities = 61/178 (34%), Positives = 85/178 (47%), Gaps = 23/178 (12%)
Query: 1 MHKSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVEHGENFNVLH 60
+ ++ + + TG R S A+L D +V I RI D T + V E V +
Sbjct: 360 LRRATISNPVTGALETVHYRISKSAWLSGYEDPVVSRINMRIQDLTGLDVSTAEELQVAN 419
Query: 61 YEVGQKYEPHYDYFM----DTFSTTYAGQRIATMLMYLSDVEEGGETVFPNAKGNFSSVP 116
Y VG +YEPH+D+ D F G RIAT L Y+SDV GG TVFP
Sbjct: 420 YGVGGQYEPHFDFARKDEPDAFRELGTGNRIATWLFYMSDVSAGGATVFPEV-------- 471
Query: 117 WWNELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMH 174
G S+ PK G A+ ++++ D S+ H ACPV+ G+KW+ KW+H
Sbjct: 472 -----------GASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLH 518
>P4H1_HUMAN (P13674) Prolyl 4-hydroxylase alpha-1 subunit precursor
(EC 1.14.11.2) (4-PH alpha-1)
(Procollagen-proline,2-oxoglutarate-4-dioxygenase
alpha-1 subunit)
Length = 534
Score = 105 bits (262), Expect = 6e-23
Identities = 60/178 (33%), Positives = 86/178 (47%), Gaps = 23/178 (12%)
Query: 1 MHKSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVEHGENFNVLH 60
+ ++ + + TG+ R S A+L + +V I RI D T + V E V +
Sbjct: 360 LRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVAN 419
Query: 61 YEVGQKYEPHYDYFM----DTFSTTYAGQRIATMLMYLSDVEEGGETVFPNAKGNFSSVP 116
Y VG +YEPH+D+ D F G RIAT L Y+SDV GG TVFP
Sbjct: 420 YGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEV-------- 471
Query: 117 WWNELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMH 174
G S+ PK G A+ ++++ D S+ H ACPV+ G+KW+ KW+H
Sbjct: 472 -----------GASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLH 518
>P4H2_HUMAN (O15460) Prolyl 4-hydroxylase alpha-2 subunit precursor
(EC 1.14.11.2) (4-PH alpha-2)
(Procollagen-proline,2-oxoglutarate-4-dioxygenase
alpha-2 subunit) (UNQ290/PRO330)
Length = 535
Score = 105 bits (261), Expect = 7e-23
Identities = 60/176 (34%), Positives = 88/176 (49%), Gaps = 23/176 (13%)
Query: 3 KSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVEHGENFNVLHYE 62
++ V D +TG + R S ++L+ D +V + RR+ T + V+ E V +Y
Sbjct: 363 RATVRDPKTGVLTVASYRVSKSSWLEEDDDPVVARVNRRMQHITGLTVKTAELLQVANYG 422
Query: 63 VGQKYEPHYDYF----MDTFSTTYAGQRIATMLMYLSDVEEGGETVFPNAKGNFSSVPWW 118
VG +YEPH+D+ DTF G R+AT L Y+SDVE GG TVFP+
Sbjct: 423 VGGQYEPHFDFSRNDERDTFKHLGTGNRVATFLNYMSDVEAGGATVFPDL---------- 472
Query: 119 NELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMH 174
G +I PK G A+ ++++ D + H ACPV+ G KW+ KW H
Sbjct: 473 ---------GAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKWVSNKWFH 519
>P4H2_MOUSE (Q60716) Prolyl 4-hydroxylase alpha-2 subunit precursor
(EC 1.14.11.2) (4-PH alpha-2)
(Procollagen-proline,2-oxoglutarate-4-dioxygenase
alpha-2 subunit)
Length = 537
Score = 102 bits (254), Expect = 5e-22
Identities = 58/176 (32%), Positives = 87/176 (48%), Gaps = 23/176 (13%)
Query: 3 KSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVEHGENFNVLHYE 62
++ V D +TG + R S ++L+ D +V + RR+ T + V+ E V +Y
Sbjct: 365 RATVRDPKTGVLTVASYRVSKSSWLEEDDDPVVARVNRRMQHITGLTVKTAELLQVANYG 424
Query: 63 VGQKYEPHYDYFM----DTFSTTYAGQRIATMLMYLSDVEEGGETVFPNAKGNFSSVPWW 118
+G +YEPH+D+ D F G R+AT L Y+SDVE GG TVFP+
Sbjct: 425 MGGQYEPHFDFSRSDDEDAFKRLGTGNRVATFLNYMSDVEAGGATVFPDL---------- 474
Query: 119 NELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMH 174
G +I PK G A+ ++++ D + H ACPV+ G KW+ KW H
Sbjct: 475 ---------GAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKWVSNKWFH 521
>P4H2_CAEEL (Q20065) Prolyl 4-hydroxylase alpha-2 subunit precursor
(EC 1.14.11.2) (4-PH alpha-2)
(Procollagen-proline,2-oxoglutarate-4-dioxygenase
alpha-2 subunit)
Length = 539
Score = 99.4 bits (246), Expect = 4e-21
Identities = 57/178 (32%), Positives = 89/178 (49%), Gaps = 23/178 (12%)
Query: 1 MHKSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVEHGENFNVLH 60
+ ++ V + +TG + R S A+LK D ++ + RRI DFT + E V +
Sbjct: 350 LKRATVQNSKTGELEHATYRISKSAWLKGDLDPVIDRVNRRIEDFTNLNQATSEELQVAN 409
Query: 61 YEVGQKYEPHYDYF----MDTFSTTYAGQRIATMLMYLSDVEEGGETVFPNAKGNFSSVP 116
Y +G Y+PH+D+ + F T G RIAT+L Y+S E GG TVF
Sbjct: 410 YGLGGHYDPHFDFARKEEKNAFKTLNTGNRIATVLFYMSQPERGGATVF----------- 458
Query: 117 WWNELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMH 174
N L G ++ P +A+ +++++ D D + H ACPV+ G KW+ KW+H
Sbjct: 459 --NHL------GTAVFPSKNDALFWYNLRRDGEGDLRTRHAACPVLLGVKWVSNKWIH 508
>P4H1_CAEEL (Q10576) Prolyl 4-hydroxylase alpha-1 subunit precursor
(EC 1.14.11.2) (4-PH alpha-1)
(Procollagen-proline,2-oxoglutarate-4-dioxygenase
alpha-1 subunit)
Length = 559
Score = 95.1 bits (235), Expect = 8e-20
Identities = 53/176 (30%), Positives = 85/176 (48%), Gaps = 23/176 (13%)
Query: 3 KSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVEHGENFNVLHYE 62
++ V D TG V + R S A+LK +V+ + +RI T + +E E + +Y
Sbjct: 355 RATVHDSVTGKLVTATYRISKSAWLKEWEGDVVETVNKRIGYMTNLEMETAEELQIANYG 414
Query: 63 VGQKYEPHYDYF----MDTFSTTYAGQRIATMLMYLSDVEEGGETVFPNAKGNFSSVPWW 118
+G Y+PH+D+ +F + G RIAT+L Y+S GG TVF AK
Sbjct: 415 IGGHYDPHFDHAKKEESKSFESLGTGNRIATVLFYMSQPSHGGGTVFTEAKS-------- 466
Query: 119 NELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMH 174
+I P +A+ ++++ +P + H ACPV+ G KW+ KW+H
Sbjct: 467 -----------TILPTKNDALFWYNLYKQGDGNPDTRHAACPVLVGIKWVSNKWIH 511
>EGLX_MOUSE (Q8BG58) Putative HIF-prolyl hydroxylase PH-4 (EC
1.14.11.-) (Hypoxia-inducible factor prolyl
4-hydroxylase)
Length = 503
Score = 72.8 bits (177), Expect = 4e-13
Identities = 53/176 (30%), Positives = 80/176 (45%), Gaps = 30/176 (17%)
Query: 30 GSDRIVKNIERRIADFTFIP---VEHGENFNVLHYEVGQKYEPHYD---YFMDTFSTTY- 82
G+ +++ I +R+ T + VE E V+ Y G Y H D + +T +
Sbjct: 286 GAHHVMRAIRQRVLRLTRLSPEIVEFSEPLQVVRYGEGGHYHAHVDSGPVYPETICSHTK 345
Query: 83 ----------AGQRIATMLMYLSDVEEGGETVFPNAKGN----FSSVPWWNELSD----C 124
R T+L YL++V GGETVFP A S + +L D C
Sbjct: 346 LVANESVPFETSCRYMTVLFYLNNVTGGGETVFPVADNRTYDEMSLIQDDVDLRDTRRHC 405
Query: 125 GKGGLSIKPKMGNAILFWSMKPDAT-----LDPSSLHGACPVIKGDKWLCAKWMHV 175
KG L +KP+ G A+ +++ PD +D SLHG C V +G KW+ W++V
Sbjct: 406 DKGNLRVKPQQGTAVFWYNYLPDGQGWVGEVDDYSLHGGCLVTRGTKWIANNWINV 461
>EGLX_HUMAN (Q9NXG6) Putative HIF-prolyl hydroxylase PH-4 (EC
1.14.11.-) (Hypoxia-inducible factor prolyl
4-hydroxylase)
Length = 502
Score = 72.4 bits (176), Expect = 5e-13
Identities = 54/176 (30%), Positives = 80/176 (44%), Gaps = 30/176 (17%)
Query: 30 GSDRIVKNIERRIADFTFIP---VEHGENFNVLHYEVGQKYEPHYD---YFMDTFSTTY- 82
G+ I++ I +R+ T + VE E V+ Y G Y H D + +T +
Sbjct: 285 GAHHIMRAIRQRVLRLTRLSPEIVELSEPLQVVRYGEGGHYHAHVDSGPVYPETICSHTK 344
Query: 83 ----------AGQRIATMLMYLSDVEEGGETVFPNAKGN----FSSVPWWNELSD----C 124
R T+L YL++V GGETVFP A S + +L D C
Sbjct: 345 LVANESVPFETSCRYMTVLFYLNNVTGGGETVFPVADNRTYDEMSLIQDDVDLRDTRRHC 404
Query: 125 GKGGLSIKPKMGNAILFWSMKPDAT-----LDPSSLHGACPVIKGDKWLCAKWMHV 175
KG L +KP+ G A+ +++ PD +D SLHG C V +G KW+ W++V
Sbjct: 405 DKGNLRVKPQQGTAVFWYNYLPDGQGWVGDVDDYSLHGGCLVTRGTKWIANNWINV 460
>XA5L_MOUSE (Q9WTJ8) XAP-5-like protein
Length = 334
Score = 29.3 bits (64), Expect = 5.2
Identities = 22/66 (33%), Positives = 33/66 (49%), Gaps = 4/66 (6%)
Query: 4 SAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIP-VEHGENFNVLHYE 62
S +DEE G+ DSR+ S+ A + KN + D +F+P E E N L E
Sbjct: 121 SFTLDEEEGDQEDSRQAESAEAHSAGAKKNLGKNPD---VDTSFLPDREREEEENRLREE 177
Query: 63 VGQKYE 68
+ Q++E
Sbjct: 178 LRQEWE 183
>V006_FOWPV (P14361) Hypothetical protein FPV006/FPV255 (BamHI-ORF1)
Length = 418
Score = 29.3 bits (64), Expect = 5.2
Identities = 18/48 (37%), Positives = 25/48 (51%), Gaps = 6/48 (12%)
Query: 58 VLHYEVGQKYEPHYDYFMDTFSTTYAGQRIAT-MLMYLSDVEEGGETV 104
++ YE G + H D FST ++ I +L+YL E GGETV
Sbjct: 107 LIMYEKGDYFARHRD-----FSTVFSKNIICVHLLLYLEQPETGGETV 149
>SYIC_SCHPO (O13651) Isoleucyl-tRNA synthetase, cytoplasmic (EC
6.1.1.5) (Isoleucine--tRNA ligase) (IleRS)
Length = 1064
Score = 29.3 bits (64), Expect = 5.2
Identities = 19/41 (46%), Positives = 23/41 (55%), Gaps = 8/41 (19%)
Query: 64 GQKYEPHYDYFMDTFSTTYAGQRIATMLMYLSD-VEEGGET 103
GQKYEP + YF TF G+R +Y +D VEEG T
Sbjct: 282 GQKYEPLFPYFKSTF-----GER--AFKLYSADYVEEGSGT 315
>SFL1_YEAST (P20134) Flocculation suppression protein (SFL1 protein)
Length = 766
Score = 28.9 bits (63), Expect = 6.7
Identities = 20/72 (27%), Positives = 37/72 (50%), Gaps = 11/72 (15%)
Query: 2 HKSAVIDEETGNGVDSRERT----------SSGAFLKRGSDRIVKNIERRIADFTFIPVE 51
+K++ DE TG GV +E++ SSG F K+G +K+I+RR + +
Sbjct: 157 NKNSSGDENTGGGVQEKEKSNPTKIWEFKHSSGIF-KKGDIEGLKHIKRRASSRNNSSIN 215
Query: 52 HGENFNVLHYEV 63
+N + +Y++
Sbjct: 216 SRKNSSNQNYDI 227
>ER1A_HUMAN (Q96HE7) ERO1-like protein alpha precursor (EC 1.8.4.-)
(ERO1-Lalpha) (Oxidoreductin 1-lalpha) (Endoplasmic
oxidoreductin 1-like protein) (ERO1-L) (UNQ434/PRO865)
Length = 468
Score = 28.5 bits (62), Expect = 8.8
Identities = 8/18 (44%), Positives = 14/18 (77%)
Query: 116 PWWNELSDCGKGGLSIKP 133
P+WN++S CG+ ++KP
Sbjct: 86 PFWNDISQCGRRDCAVKP 103
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.318 0.137 0.431
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,057,094
Number of Sequences: 164201
Number of extensions: 1052293
Number of successful extensions: 2115
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 2077
Number of HSP's gapped (non-prelim): 15
length of query: 180
length of database: 59,974,054
effective HSP length: 103
effective length of query: 77
effective length of database: 43,061,351
effective search space: 3315724027
effective search space used: 3315724027
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 62 (28.5 bits)
Medicago: description of AC149547.9