BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148528.1 + phase: 0
(131 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TWST_XENLA (P13903) Twist related protein (T18) (X-twist) 36 0.013
HAS1_XENLA (P13563) Hyaluronan synthase 1 (EC 2.4.1.212) (Hyalur... 34 0.064
ODO2_BUCAI (P57389) Dihydrolipoyllysine-residue succinyltransfer... 32 0.32
MAD1_SCHPO (P87169) Spindle assembly checkpoint component mad1 (... 32 0.32
ARRS_MAIZE (P13027) Anthocyanin regulatory R-S protein 32 0.32
ARLC_MAIZE (P13526) Anthocyanin regulatory Lc protein 32 0.32
P200_MYCGE (Q49429) Protein P200 31 0.54
DPO3_MYCGE (P47277) DNA polymerase III polC-type (EC 2.7.7.7) (P... 30 0.71
YHES_HAEIN (P44808) Probable ABC transporter ATP-binding protein... 30 0.93
MYCN_XENLA (P24793) N-myc protein 30 0.93
CALD_HUMAN (Q05682) Caldesmon (CDM) 30 0.93
CALD_RAT (Q62736) Non-muscle caldesmon (CDM) (L-caldesmon) 30 1.2
SYR_CHLTE (Q8KGF3) Arginyl-tRNA synthetase (EC 6.1.1.19) (Argini... 29 1.6
ESC1_SCHPO (Q04635) Protein esc1 29 1.6
A2S3_HUMAN (O60296) Amyotrophic lateral sclerosis 2 chromosomal ... 29 1.6
SYS_SULSO (O33780) Seryl-tRNA synthetase (EC 6.1.1.11) (Serine--... 29 2.1
EGL1_ARATH (Q9CAD0) Transcription factor EGL1 (ENHANCER OF GLABR... 29 2.1
UCR7_SCHPO (O74533) Probable ubiquinol-cytochrome C reductase co... 28 2.7
PUSH_ARATH (O22928) Putative pseudouridylate synthase (EC 4.2.1.... 28 2.7
KKK1_YEAST (P34244) Probable serine/threonine-protein kinase YKL... 28 2.7
>TWST_XENLA (P13903) Twist related protein (T18) (X-twist)
Length = 166
Score = 36.2 bits (82), Expect = 0.013
Identities = 24/96 (25%), Positives = 42/96 (43%), Gaps = 7/96 (7%)
Query: 25 LPNSDHNLGESSKKRVDTKPLNQNEGINEAEAPVSKKRNWGGVVIRSENNITTGEEKDKK 84
L NS+ L + KR K + + + ++P+S KRN + + T + +
Sbjct: 16 LSNSEEELDKQQSKRGCRKRRSARKSPEDPDSPISVKRN-------KKASSTGSSPQSFE 68
Query: 85 YRDFDHEMHILTERERRKKMRNMFDSLHALLPELPS 120
M + ER+R + + F SL ++P LPS
Sbjct: 69 ELQSQRVMANVRERQRTQSLNEAFSSLRKIIPTLPS 104
>HAS1_XENLA (P13563) Hyaluronan synthase 1 (EC 2.4.1.212)
(Hyaluronate synthase 1) (Hyaluronic acid synthase 1)
(HA synthase 1) (XHAS1) (DG42 protein)
Length = 588
Score = 33.9 bits (76), Expect = 0.064
Identities = 24/72 (33%), Positives = 34/72 (46%), Gaps = 10/72 (13%)
Query: 1 MAKSVDHDQGVQTGFIWENHLWGDLPNSDHNLGESSKKRVDTKPLNQNEGINEAEAPVSK 60
M K V H + V T ++W+ + + N G + +KPLN++EGIN E V
Sbjct: 150 MFKDVFHGEDVGT-YVWKGNYHTVKKPEETNKGSCPEV---SKPLNEDEGINMVEELVRN 205
Query: 61 KR------NWGG 66
KR WGG
Sbjct: 206 KRCVCIMQQWGG 217
>ODO2_BUCAI (P57389) Dihydrolipoyllysine-residue succinyltransferase
component of 2-oxoglutarate dehydrogenase complex (EC
2.3.1.61) (E2) (Dihydrolipoamide succinyltransferase
component of 2-oxoglutarate dehydrogenase complex)
Length = 420
Score = 31.6 bits (70), Expect = 0.32
Identities = 26/101 (25%), Positives = 42/101 (40%), Gaps = 10/101 (9%)
Query: 23 GDLPNSDHNLGESSKKRVDTKPLNQNEGINEAEAPVSKKRNWGGVVIRSENNITTGEEKD 82
G + S LGE +K V L+ N I + + + K+ E ITT E+K+
Sbjct: 65 GKVVISQQTLGEINKSTVVDNHLSNNHIIEKEDNLLKKE----------EKYITTEEKKE 114
Query: 83 KKYRDFDHEMHILTERERRKKMRNMFDSLHALLPELPSKVN 123
+Y D+ H+ R K+ N+ + + E K N
Sbjct: 115 IEYLLKDNHKHLTPSMRRSVKIHNINNGFLNQVIETSKKTN 155
>MAD1_SCHPO (P87169) Spindle assembly checkpoint component mad1
(Mitotic arrest deficient protein 1)
Length = 689
Score = 31.6 bits (70), Expect = 0.32
Identities = 30/117 (25%), Positives = 52/117 (43%), Gaps = 12/117 (10%)
Query: 25 LPNSDHNLGE---SSKKRVDTKPLNQNEGINEAEA-------PVSKKRNWGGVVIRSENN 74
L N++ NLGE S + ++ KP NQ G NE +A K+ + ++ + +
Sbjct: 329 LQNTNANLGERVSSLESQLSNKPANQPLGANEKDAAHITELETKLKELHEQNRRLQRQKS 388
Query: 75 ITTGEEK--DKKYRDFDHEMHILTERERRKKMRNMFDSLHALLPELPSKVNSFSSSI 129
+ T E + + +D E IL+E+ K + L L+ E K+ S S+
Sbjct: 389 LATQEIDLLRENLKSYDDEEAILSEKNTDMKKLERIEGLVKLVDEYKLKLESMPVSL 445
>ARRS_MAIZE (P13027) Anthocyanin regulatory R-S protein
Length = 612
Score = 31.6 bits (70), Expect = 0.32
Identities = 15/34 (44%), Positives = 24/34 (70%), Gaps = 1/34 (2%)
Query: 93 HILTERERRKKMRNMFDSLHALLPELPSKVNSFS 126
H+++ER+RR+K+ MF L +LLP + +VN S
Sbjct: 419 HVMSERKRREKLNEMFLVLKSLLPSI-HRVNKAS 451
>ARLC_MAIZE (P13526) Anthocyanin regulatory Lc protein
Length = 610
Score = 31.6 bits (70), Expect = 0.32
Identities = 15/34 (44%), Positives = 24/34 (70%), Gaps = 1/34 (2%)
Query: 93 HILTERERRKKMRNMFDSLHALLPELPSKVNSFS 126
H+++ER+RR+K+ MF L +LLP + +VN S
Sbjct: 417 HVMSERKRREKLNEMFLVLKSLLPSI-HRVNKAS 449
>P200_MYCGE (Q49429) Protein P200
Length = 1616
Score = 30.8 bits (68), Expect = 0.54
Identities = 16/60 (26%), Positives = 32/60 (52%), Gaps = 2/60 (3%)
Query: 70 RSENNITTGEEKDKKYRDFDHEMHILTERERRKKMRNMFDS--LHALLPELPSKVNSFSS 127
+++N TT + K KKY + ++ + ++ + K+ N+FD L + + K SF+S
Sbjct: 6 KNKNKNTTPKSKTKKYLESANKKSVTKPKKEQDKVENLFDQPFLGEIKKNILKKTKSFNS 65
>DPO3_MYCGE (P47277) DNA polymerase III polC-type (EC 2.7.7.7)
(PolIII)
Length = 1451
Score = 30.4 bits (67), Expect = 0.71
Identities = 17/57 (29%), Positives = 31/57 (53%), Gaps = 5/57 (8%)
Query: 24 DLPNSDHNLGESSKKRVDT---KPLNQNEGINEAE--APVSKKRNWGGVVIRSENNI 75
++PN+ L S +KRV+ + +GIN+ E A +K+R W + + ++NI
Sbjct: 314 EIPNNYKRLDLSKQKRVELVFHTKMTAFDGINDIEEYAQFAKERGWKAITVTDKDNI 370
>YHES_HAEIN (P44808) Probable ABC transporter ATP-binding protein
HI0658
Length = 638
Score = 30.0 bits (66), Expect = 0.93
Identities = 17/62 (27%), Positives = 34/62 (54%), Gaps = 1/62 (1%)
Query: 71 SENNITTGEEKDKKYRDFDHEMHILTERERRK-KMRNMFDSLHALLPELPSKVNSFSSSI 129
SE N T+ + +K D ++ + E++RR+ ++R L + +L K+N FSS +
Sbjct: 524 SEQNSTSENKVSEKVGDNENSVQNRKEQKRREAELRQQTAPLRKKITQLEEKMNKFSSEL 583
Query: 130 SS 131
++
Sbjct: 584 AN 585
>MYCN_XENLA (P24793) N-myc protein
Length = 437
Score = 30.0 bits (66), Expect = 0.93
Identities = 22/72 (30%), Positives = 33/72 (45%), Gaps = 9/72 (12%)
Query: 53 EAEAPVSKKRNWGGVVIRSENNITTGEEKDKKYRDFDHE------MHILTERERRKKMRN 106
E P K RN + R N+ + K R++D E H + ER+RR +R+
Sbjct: 316 EVAPPQKKLRN---ELPRLVKNVVPTKPKSSSPRNYDSEDSERRRNHNILERQRRNDLRS 372
Query: 107 MFDSLHALLPEL 118
F +L +PEL
Sbjct: 373 SFLTLRDHVPEL 384
>CALD_HUMAN (Q05682) Caldesmon (CDM)
Length = 793
Score = 30.0 bits (66), Expect = 0.93
Identities = 20/70 (28%), Positives = 31/70 (43%), Gaps = 1/70 (1%)
Query: 33 GESSKKRVDTKPLNQNEGINEAEAPVSKKRNWGGVVIRSENNITTGEEKDKKYRDFDHEM 92
GE+ + + Q E E E + KKR V+ E EE D+K R+ + +
Sbjct: 544 GETESEEFEKLKQKQQEAALELEE-LKKKREERRKVLEEEEQRRKQEEADRKLREEEEKR 602
Query: 93 HILTERERRK 102
+ E ERR+
Sbjct: 603 RLKEEIERRR 612
>CALD_RAT (Q62736) Non-muscle caldesmon (CDM) (L-caldesmon)
Length = 531
Score = 29.6 bits (65), Expect = 1.2
Identities = 20/70 (28%), Positives = 31/70 (43%), Gaps = 1/70 (1%)
Query: 33 GESSKKRVDTKPLNQNEGINEAEAPVSKKRNWGGVVIRSENNITTGEEKDKKYRDFDHEM 92
GE+ + + Q E E E + KKR V+ E EE D+K R+ + +
Sbjct: 283 GETESEEFEKLKQKQQEAALELEE-LKKKREERRKVLEEEEQRRKQEEADRKAREEEEKR 341
Query: 93 HILTERERRK 102
+ E ERR+
Sbjct: 342 RLKEEIERRR 351
>SYR_CHLTE (Q8KGF3) Arginyl-tRNA synthetase (EC 6.1.1.19)
(Arginine--tRNA ligase) (ArgRS)
Length = 551
Score = 29.3 bits (64), Expect = 1.6
Identities = 15/47 (31%), Positives = 22/47 (45%)
Query: 69 IRSENNITTGEEKDKKYRDFDHEMHILTERERRKKMRNMFDSLHALL 115
I ++ I + DKK+ DF + L +E RK R + L LL
Sbjct: 18 IETDKEIQIDKPNDKKFGDFSTNIAFLVAKEARKNPRELAGQLIGLL 64
>ESC1_SCHPO (Q04635) Protein esc1
Length = 413
Score = 29.3 bits (64), Expect = 1.6
Identities = 12/24 (50%), Positives = 17/24 (70%)
Query: 93 HILTERERRKKMRNMFDSLHALLP 116
H L ER+RRK+++ +FD L LP
Sbjct: 339 HKLAERKRRKEIKELFDDLKDALP 362
>A2S3_HUMAN (O60296) Amyotrophic lateral sclerosis 2 chromosomal
region candidate gene protein 3
Length = 914
Score = 29.3 bits (64), Expect = 1.6
Identities = 21/90 (23%), Positives = 38/90 (41%), Gaps = 23/90 (25%)
Query: 11 VQTGFIWENHLWGDLPNSDHNLGESSKKRVDTKPLNQNEGINEAEAPVSKKRNWGGVVIR 70
V T F++EN W P+ Q + ++A +PV + + +++
Sbjct: 58 VDTLFLYENQDWTQSPH-------------------QRQHASDALSPVLAEETFRYMILG 98
Query: 71 SENNITTGEEKDKKYRDFDHEMHILTERER 100
++ E+ K Y D D H+L ER+R
Sbjct: 99 TDRV----EQMTKTYNDIDMVTHLLAERDR 124
>SYS_SULSO (O33780) Seryl-tRNA synthetase (EC 6.1.1.11)
(Serine--tRNA ligase) (SerRS)
Length = 457
Score = 28.9 bits (63), Expect = 2.1
Identities = 17/52 (32%), Positives = 31/52 (58%), Gaps = 1/52 (1%)
Query: 68 VIRSENNITTGEEKDKKYRDFDHEMHILTERERR-KKMRNMFDSLHALLPEL 118
V+ S+ + +GEE+ KK + + + IL E+E+ +++ N D L + LP L
Sbjct: 56 VLSSQISKLSGEERKKKIEESKNLLKILEEKEKELEEIENERDRLLSSLPNL 107
>EGL1_ARATH (Q9CAD0) Transcription factor EGL1 (ENHANCER OF GLABRA3)
(Basic helix-loop-helix protein 2) (bHLH2) (AtbHLH002)
(AtMyc-146)
Length = 596
Score = 28.9 bits (63), Expect = 2.1
Identities = 12/34 (35%), Positives = 24/34 (70%), Gaps = 1/34 (2%)
Query: 93 HILTERERRKKMRNMFDSLHALLPELPSKVNSFS 126
H L+E++RR+K+ F +L +++P + SK++ S
Sbjct: 406 HALSEKKRREKLNERFMTLRSIIPSI-SKIDKVS 438
>UCR7_SCHPO (O74533) Probable ubiquinol-cytochrome C reductase
complex 14 kDa protein (EC 1.10.2.2) (Complex III
subunit VII)
Length = 137
Score = 28.5 bits (62), Expect = 2.7
Identities = 23/93 (24%), Positives = 37/93 (39%), Gaps = 7/93 (7%)
Query: 39 RVDTKPLNQNEGINEAEAPVSKKRNWGGVV-------IRSENNITTGEEKDKKYRDFDHE 91
R D L +N+ +A + + K ++ V + EN I E K D+ +
Sbjct: 39 RYDDLMLEENDDTQKALSRLPKMESYDRVYRIRRAMQLSIENKILPKSEWTKPEEDYHYL 98
Query: 92 MHILTERERRKKMRNMFDSLHALLPELPSKVNS 124
+L E +K R FD+L PE + S
Sbjct: 99 RPVLAEVIAERKEREAFDALIVKKPETQAHATS 131
>PUSH_ARATH (O22928) Putative pseudouridylate synthase (EC 4.2.1.70)
(Pseudouridine synthase)
Length = 510
Score = 28.5 bits (62), Expect = 2.7
Identities = 21/68 (30%), Positives = 35/68 (50%), Gaps = 4/68 (5%)
Query: 30 HNLGESSK-KRVDTKPLNQNEGINEAEAPVSKKRNWGGVVIRSENNITTGEEKDKKYRDF 88
HN + S+ +R + + Q G E P S K + R EN+I GEE+++K D
Sbjct: 231 HNYTQRSRYRRKSEQKIKQRNGRPPRE-PKSIKASESE--FREENHIEIGEEEEEKEVDG 287
Query: 89 DHEMHILT 96
+ + H++T
Sbjct: 288 ESDEHVVT 295
>KKK1_YEAST (P34244) Probable serine/threonine-protein kinase YKL101W
(EC 2.7.1.37)
Length = 1518
Score = 28.5 bits (62), Expect = 2.7
Identities = 22/73 (30%), Positives = 35/73 (47%), Gaps = 5/73 (6%)
Query: 34 ESSKKRVDTKPLNQN-EGINEAEAPVSKKRNWGGVVIR---SENNITTGEEKDKKYRDFD 89
ES+ + KP+ ++ + + EAP S K+NW + + S NN T + FD
Sbjct: 1367 ESNTQTHTKKPILKSVQNVEVEEAPSSDKKNWFVKLFQNFSSHNNATKASKNHVTNISFD 1426
Query: 90 HEMHILTERERRK 102
+ H+LT E K
Sbjct: 1427 -DAHMLTLNEFNK 1438
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.310 0.129 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,180,565
Number of Sequences: 164201
Number of extensions: 651896
Number of successful extensions: 1494
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 50
Number of HSP's that attempted gapping in prelim test: 1470
Number of HSP's gapped (non-prelim): 71
length of query: 131
length of database: 59,974,054
effective HSP length: 107
effective length of query: 24
effective length of database: 42,404,547
effective search space: 1017709128
effective search space used: 1017709128
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC148528.1