BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148487.1 - phase: 1
(63 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ITH5_CUCMA (P19873) Inhibitor of trypsin and hageman factor (CMT... 77 6e-15
ICI_LINUS (P82381) Proteinase inhibitor (LUTI) 74 7e-14
BGIA_MOMCH (P24076) Glu S.griseus protease inhibitor (BGIA) 73 1e-13
ATSI_AMACA (P80211) Trypsin/subtilisin inhibitor (ATSI) 62 2e-10
ITR1_NICSY (Q02214) Trypsin inhibitor I precursor (NSTI-I) 57 7e-09
IER1_LYCES (P20076) Ethylene-responsive proteinase inhibitor I p... 55 3e-08
ICI1_LYCPE (P16231) Wound-induced proteinase inhibitor I precursor 54 1e-07
ICI1_SOLTU (Q00783) Proteinase inhibitor I precursor 53 1e-07
IPIB_TOBAC (Q03199) Proteinase inhibitor I-B precursor (PI-IB) (... 52 3e-07
ICI2_HORVU (P01053) Subtilisin-chymotrypsin inhibitor-2A (CI-2A) 52 4e-07
ICI1_LYCES (P05118) Wound-induced proteinase inhibitor I precursor 52 4e-07
IPIA_TOBAC (Q03198) Proteinase inhibitor I-A precursor (PI-IA) (... 51 5e-07
ICID_SOLTU (P08454) Wound-induced proteinase inhibitor I precurs... 51 5e-07
ICI_LUMTE (P83472) Chymotrypsin inhibitor precursor (LTCI) 50 8e-07
ICIA_SOLTU (P01052) Chymotrypsin inhibitor I, A, B and C subunits 49 2e-06
ICIC_HORVU (P01054) Subtilisin-chymotrypsin inhibitor CI-1C 49 2e-06
ICI3_HORVU (P08626) Subtilisin-chymotrypsin inhibitor-2B (CI-2B)... 47 7e-06
ICI1_CANLI (P81712) Subtilisin inhibitor CLSI-I 47 1e-05
ICIW_WHEAT (P82977) Subtilisin-chymotrypsin inhibitor WSCI 46 2e-05
ICI1_PHAAN (P16064) Subtilisin inhibitor I (ASI-I) [Contains: Su... 45 3e-05
>ITH5_CUCMA (P19873) Inhibitor of trypsin and hageman factor
(CMTI-V)
Length = 68
Score = 77.4 bits (189), Expect = 6e-15
Identities = 36/63 (57%), Positives = 46/63 (72%)
Query: 1 KSSWPELVGVEGKVAEATIQRENPLVDAIIVPEGSSVPFDFRCDRVWVWINKDEIVYQVP 60
KSSWP LVGV G VA+A I+R+NP V A+I+ EG+ V DFRC+RV +W+NK +V P
Sbjct: 6 KSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSPP 65
Query: 61 TIG 63
IG
Sbjct: 66 RIG 68
>ICI_LINUS (P82381) Proteinase inhibitor (LUTI)
Length = 69
Score = 73.9 bits (180), Expect = 7e-14
Identities = 34/62 (54%), Positives = 45/62 (71%)
Query: 1 KSSWPELVGVEGKVAEATIQRENPLVDAIIVPEGSSVPFDFRCDRVWVWINKDEIVYQVP 60
K++WPELVG G +A AT++REN V AI++ EGS++ DFRCDRVWV +N +V VP
Sbjct: 7 KNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTSVP 66
Query: 61 TI 62
I
Sbjct: 67 HI 68
>BGIA_MOMCH (P24076) Glu S.griseus protease inhibitor (BGIA)
Length = 68
Score = 73.2 bits (178), Expect = 1e-13
Identities = 36/63 (57%), Positives = 43/63 (68%)
Query: 1 KSSWPELVGVEGKVAEATIQRENPLVDAIIVPEGSSVPFDFRCDRVWVWINKDEIVYQVP 60
K SWP+LVG G A+A I+RENP V A+IV GS V DFRCDRV VW+ + IV + P
Sbjct: 6 KRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVARPP 65
Query: 61 TIG 63
IG
Sbjct: 66 AIG 68
>ATSI_AMACA (P80211) Trypsin/subtilisin inhibitor (ATSI)
Length = 69
Score = 62.4 bits (150), Expect = 2e-10
Identities = 30/62 (48%), Positives = 38/62 (60%)
Query: 1 KSSWPELVGVEGKVAEATIQRENPLVDAIIVPEGSSVPFDFRCDRVWVWINKDEIVYQVP 60
K WPELVG G A A I+RENP V +I+ E S DFRCDRVWV ++ +V + P
Sbjct: 7 KQEWPELVGEYGYKAAAIIERENPNVRSIVKHERSGFTKDFRCDRVWVVVDSTGVVVRTP 66
Query: 61 TI 62
+
Sbjct: 67 RV 68
>ITR1_NICSY (Q02214) Trypsin inhibitor I precursor (NSTI-I)
Length = 94
Score = 57.4 bits (137), Expect = 7e-09
Identities = 29/64 (45%), Positives = 40/64 (62%), Gaps = 1/64 (1%)
Query: 1 KSSWPELVGVEGKVAEATIQRENP-LVDAIIVPEGSSVPFDFRCDRVWVWINKDEIVYQV 59
K +WPEL+GV K A IQ+EN L + V GS V DFRC+RV +++N + V Q+
Sbjct: 31 KETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLDFVVQI 90
Query: 60 PTIG 63
P +G
Sbjct: 91 PRVG 94
>IER1_LYCES (P20076) Ethylene-responsive proteinase inhibitor I
precursor
Length = 119
Score = 55.5 bits (132), Expect = 3e-08
Identities = 28/64 (43%), Positives = 40/64 (61%), Gaps = 1/64 (1%)
Query: 1 KSSWPELVGVEGKVAEATIQRENP-LVDAIIVPEGSSVPFDFRCDRVWVWINKDEIVYQV 59
K SWPEL+G K A+ IQ+ENP L + + GS+ D RC+RV +++N +IV Q
Sbjct: 56 KESWPELLGTPAKFAKQIIQKENPKLTNVETLLNGSAFTEDLRCNRVRLFVNLLDIVVQT 115
Query: 60 PTIG 63
P +G
Sbjct: 116 PKVG 119
>ICI1_LYCPE (P16231) Wound-induced proteinase inhibitor I precursor
Length = 111
Score = 53.5 bits (127), Expect = 1e-07
Identities = 28/63 (44%), Positives = 39/63 (61%), Gaps = 1/63 (1%)
Query: 1 KSSWPELVGVEGKVAEATIQRENPLVDAI-IVPEGSSVPFDFRCDRVWVWINKDEIVYQV 59
K WPEL+GV A+ I++ENP + I I+ GS V DFRCDRV +++N V Q+
Sbjct: 48 KQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQI 107
Query: 60 PTI 62
P +
Sbjct: 108 PRV 110
>ICI1_SOLTU (Q00783) Proteinase inhibitor I precursor
Length = 107
Score = 53.1 bits (126), Expect = 1e-07
Identities = 27/64 (42%), Positives = 39/64 (60%), Gaps = 1/64 (1%)
Query: 1 KSSWPELVGVEGKVAEATIQRENPLVDAI-IVPEGSSVPFDFRCDRVWVWINKDEIVYQV 59
K WPEL+GV K+A+ I++EN L+ + I+ GS V D RCDRV ++ N V +
Sbjct: 44 KLRWPELIGVPTKLAKGIIEKENSLISNVHILLNGSPVTLDIRCDRVRLFDNILGYVVDI 103
Query: 60 PTIG 63
P +G
Sbjct: 104 PVVG 107
>IPIB_TOBAC (Q03199) Proteinase inhibitor I-B precursor (PI-IB)
(Inhibitor of microbial serine proteinases major
isoform)
Length = 107
Score = 52.0 bits (123), Expect = 3e-07
Identities = 27/64 (42%), Positives = 37/64 (57%), Gaps = 1/64 (1%)
Query: 1 KSSWPELVGVEGKVAEATIQRENP-LVDAIIVPEGSSVPFDFRCDRVWVWINKDEIVYQV 59
K WPEL+G K A IQ+ENP L + V G+ V D RC+RV +++N + V Q
Sbjct: 44 KERWPELLGTPAKFAMQIIQKENPKLTNVQTVLNGTPVTEDLRCNRVRLFVNVLDFVVQT 103
Query: 60 PTIG 63
P +G
Sbjct: 104 PQVG 107
>ICI2_HORVU (P01053) Subtilisin-chymotrypsin inhibitor-2A (CI-2A)
Length = 83
Score = 51.6 bits (122), Expect = 4e-07
Identities = 23/63 (36%), Positives = 40/63 (62%)
Query: 1 KSSWPELVGVEGKVAEATIQRENPLVDAIIVPEGSSVPFDFRCDRVWVWINKDEIVYQVP 60
K+ WPELVG + A+ I ++ P I++P G+ V ++R DRV ++++K + + QVP
Sbjct: 21 KTEWPELVGKSVEEAKKVILQDKPEAQIIVLPVGTIVTMEYRIDRVRLFVDKLDNIAQVP 80
Query: 61 TIG 63
+G
Sbjct: 81 RVG 83
>ICI1_LYCES (P05118) Wound-induced proteinase inhibitor I precursor
Length = 111
Score = 51.6 bits (122), Expect = 4e-07
Identities = 26/63 (41%), Positives = 39/63 (61%), Gaps = 1/63 (1%)
Query: 1 KSSWPELVGVEGKVAEATIQRENPLVDAI-IVPEGSSVPFDFRCDRVWVWINKDEIVYQV 59
K WPEL+GV K+A+ I++ENP + I I+ GS + D+ CDRV ++ N V Q+
Sbjct: 48 KQMWPELIGVPTKLAKEIIEKENPSITNIPILLSGSPITLDYLCDRVRLFDNILGFVVQM 107
Query: 60 PTI 62
P +
Sbjct: 108 PVV 110
>IPIA_TOBAC (Q03198) Proteinase inhibitor I-A precursor (PI-IA)
(Inhibitor of microbial serine proteinases minor
isoform)
Length = 107
Score = 51.2 bits (121), Expect = 5e-07
Identities = 26/64 (40%), Positives = 36/64 (55%), Gaps = 1/64 (1%)
Query: 1 KSSWPELVGVEGKVAEATIQRENP-LVDAIIVPEGSSVPFDFRCDRVWVWINKDEIVYQV 59
K WPEL+G K A IQ+ENP L + + G V D RC+RV +++N + + Q
Sbjct: 44 KERWPELLGTPAKFAMQIIQKENPKLTNVQTILNGGPVTEDLRCNRVRLFVNVLDFIVQT 103
Query: 60 PTIG 63
P IG
Sbjct: 104 PQIG 107
>ICID_SOLTU (P08454) Wound-induced proteinase inhibitor I precursor
(Chymotrypsin inhibitor I, D subunit)
Length = 107
Score = 51.2 bits (121), Expect = 5e-07
Identities = 26/63 (41%), Positives = 40/63 (63%), Gaps = 1/63 (1%)
Query: 1 KSSWPELVGVEGKVAEATIQRENPLVDAI-IVPEGSSVPFDFRCDRVWVWINKDEIVYQV 59
K WPEL+GV K+A+ I++EN L+ + I+ GS V D+RC+RV ++ N V Q+
Sbjct: 44 KQRWPELIGVPTKLAKGIIEKENSLITNVQILLNGSPVTMDYRCNRVRLFDNILGDVVQI 103
Query: 60 PTI 62
P +
Sbjct: 104 PRV 106
>ICI_LUMTE (P83472) Chymotrypsin inhibitor precursor (LTCI)
Length = 86
Score = 50.4 bits (119), Expect = 8e-07
Identities = 26/63 (41%), Positives = 37/63 (58%)
Query: 1 KSSWPELVGVEGKVAEATIQRENPLVDAIIVPEGSSVPFDFRCDRVWVWINKDEIVYQVP 60
K+SWPELVG + A+A I + P + PE S V +D+R RV +++NKD V + P
Sbjct: 24 KTSWPELVGETLEEAKAQILEDRPDAVIKVQPEHSPVTYDYRPSRVIIFVNKDGNVAETP 83
Query: 61 TIG 63
G
Sbjct: 84 AAG 86
>ICIA_SOLTU (P01052) Chymotrypsin inhibitor I, A, B and C subunits
Length = 71
Score = 49.3 bits (116), Expect = 2e-06
Identities = 25/63 (39%), Positives = 40/63 (62%), Gaps = 1/63 (1%)
Query: 1 KSSWPELVGVEGKVAEATIQRENPLVDAI-IVPEGSSVPFDFRCDRVWVWINKDEIVYQV 59
K WPEL+GV K+A+ I+++N L+ + I+ GS V DFRC+RV ++ + V Q+
Sbjct: 8 KLQWPELIGVPTKLAKEIIEKQNSLISNVHILLNGSPVTMDFRCNRVRLFDDILGSVVQI 67
Query: 60 PTI 62
P +
Sbjct: 68 PRV 70
>ICIC_HORVU (P01054) Subtilisin-chymotrypsin inhibitor CI-1C
Length = 77
Score = 48.9 bits (115), Expect = 2e-06
Identities = 22/63 (34%), Positives = 38/63 (59%)
Query: 1 KSSWPELVGVEGKVAEATIQRENPLVDAIIVPEGSSVPFDFRCDRVWVWINKDEIVYQVP 60
K+SWPE+VG+ + A+ I R+ P ++P + VP +F +RV+V ++K V +V
Sbjct: 15 KTSWPEVVGMSAEKAKEIILRDKPNAQIEVIPVDAMVPLNFNPNRVFVLVHKATTVAZVS 74
Query: 61 TIG 63
+G
Sbjct: 75 RVG 77
>ICI3_HORVU (P08626) Subtilisin-chymotrypsin inhibitor-2B (CI-2B)
(Fragment)
Length = 72
Score = 47.4 bits (111), Expect = 7e-06
Identities = 21/63 (33%), Positives = 39/63 (61%)
Query: 1 KSSWPELVGVEGKVAEATIQRENPLVDAIIVPEGSSVPFDFRCDRVWVWINKDEIVYQVP 60
K+ WPELV + A+ I ++ P I++P G+ V ++R DRV +++++ + + QVP
Sbjct: 10 KTEWPELVEKSVEEAKKVILQDKPEAQIIVLPVGTIVTMEYRIDRVRLFVDRLDNIAQVP 69
Query: 61 TIG 63
+G
Sbjct: 70 RVG 72
>ICI1_CANLI (P81712) Subtilisin inhibitor CLSI-I
Length = 65
Score = 46.6 bits (109), Expect = 1e-05
Identities = 26/63 (41%), Positives = 39/63 (61%), Gaps = 1/63 (1%)
Query: 1 KSSWPELVGVEGKVAEATIQRENPLVDAIIVPEGSSVPFDFRCDRVWVWINKDEIVYQVP 60
K+SWPELVGV + AE I+ E V+ +VP GS V D++ RV +++++ V + P
Sbjct: 4 KTSWPELVGVTAEEAEK-IKEEMSGVEIQVVPPGSFVTADYKPQRVRLYVDESNKVTRTP 62
Query: 61 TIG 63
IG
Sbjct: 63 GIG 65
>ICIW_WHEAT (P82977) Subtilisin-chymotrypsin inhibitor WSCI
Length = 72
Score = 46.2 bits (108), Expect = 2e-05
Identities = 20/63 (31%), Positives = 38/63 (59%)
Query: 1 KSSWPELVGVEGKVAEATIQRENPLVDAIIVPEGSSVPFDFRCDRVWVWINKDEIVYQVP 60
K+ WPELVG + A+ I ++ +++P G+ V ++R DRV ++++ + + QVP
Sbjct: 10 KTEWPELVGKSVEEAKKVILQDKSEAQIVVLPVGTIVTMEYRIDRVRLFVDSLDKIAQVP 69
Query: 61 TIG 63
+G
Sbjct: 70 RVG 72
>ICI1_PHAAN (P16064) Subtilisin inhibitor I (ASI-I) [Contains:
Subtilisin inhibitor II (ASI-II)]
Length = 92
Score = 45.4 bits (106), Expect = 3e-05
Identities = 24/63 (38%), Positives = 36/63 (57%)
Query: 1 KSSWPELVGVEGKVAEATIQRENPLVDAIIVPEGSSVPFDFRCDRVWVWINKDEIVYQVP 60
K+SWPELVGV + AE I+ E V + P S V D+ RV +++++ V + P
Sbjct: 30 KTSWPELVGVTAEQAETKIKEEMVDVQIQVSPHDSFVTADYNPKRVRLYVDESNKVTRTP 89
Query: 61 TIG 63
+IG
Sbjct: 90 SIG 92
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.318 0.139 0.441
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,147,456
Number of Sequences: 164201
Number of extensions: 259747
Number of successful extensions: 551
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 38
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 500
Number of HSP's gapped (non-prelim): 39
length of query: 63
length of database: 59,974,054
effective HSP length: 39
effective length of query: 24
effective length of database: 53,570,215
effective search space: 1285685160
effective search space used: 1285685160
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC148487.1