BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148227.2 + phase: 0 /pseudo
(1075 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from tran... 59 9e-08
M300_ARATH (P93293) Hypothetical mitochondrial protein AtMg00300... 55 8e-07
COPI_DROME (P04146) Copia protein (Gag-int-pol protein) [Contain... 52 1e-05
DP2S_METTH (O27456) DNA polymerase II small subunit (EC 2.7.7.7)... 40 0.044
SCA4_RICAF (Q9AJ83) Antigenic heat-stable 120 kDa protein (PS120... 37 0.29
SCA4_RICCN (Q52658) Antigenic heat-stable 120 kDa protein (PS120... 37 0.37
SCA4_RICMN (Q9AJ82) Antigenic heat-stable 120 kDa protein (PS120... 35 0.83
SCA4_RICJA (Q9AJ79) Antigenic heat-stable 120 kDa protein (PS120... 35 0.83
SCA4_RICPA (Q9AJ75) Antigenic heat-stable 120 kDa protein (PS120... 35 1.1
SCA4_RICSI (Q9AJ77) Antigenic heat-stable 120 kDa protein (PS120... 33 3.2
YEAB_SCHPO (O14076) Hypothetical protein UNK4.11c in chromosome I 32 9.2
>POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from
transposon TNT 1-94 [Contains: Protease (EC 3.4.23.-);
Reverse transcriptase (EC 2.7.7.49); Endonuclease]
Length = 1328
Score = 58.5 bits (140), Expect = 9e-08
Identities = 73/296 (24%), Positives = 117/296 (38%), Gaps = 23/296 (7%)
Query: 76 VMLWHLRLGHPSFKYLKIVFPK--LFFGHDFSSFQCEIYEFSKQHRSSFPVQTYKPSKLF 133
V LWH R+GH S K L+I+ K + + + C+ F KQHR SF + + +
Sbjct: 422 VDLWHKRMGHMSEKGLQILAKKSLISYAKGTTVKPCDYCLFGKQHRVSFQTSSERKLNIL 481
Query: 134 SIIHSDVWGPNRINSYPTKDGLSPLLMIILDFIG-----YIY*KKNQR*DKLSRILLN*C 188
+++SDV GP I S G + + +D YI K+Q +
Sbjct: 482 DLVYSDVCGPMEIESM----GGNKYFVTFIDDASRKLWVYILKTKDQVFQVFQKFHALVE 537
Query: 189 RLSLIPLYKFSELTMELNILTQF*ETFFFMENGIVQQSTCVSSPQQNGITERKNRHLLEM 248
R + L + +F E + +GI + T +PQ NG+ ER NR ++E
Sbjct: 538 RETGRKLKRLRSDNGGEYTSREFEE--YCSSHGIRHEKTVPGTPQHNGVAERMNRTIVEK 595
Query: 249 ARALLFFH*SSKILMG*GCINCCTL---NKLYVISCFKP*DSSRNLLKILSKCPYFSRFA 305
R++L K G C L + ++ P N S F A
Sbjct: 596 VRSMLRMAKLPKSFWGEAVQTACYLINRSPSVPLAFEIPERVWTNKEVSYSHLKVFGCRA 655
Query: 306 FKNIRMHYFCP*TQTNRQT*TRASKRVFVGYSPTRKGYKCLDLNSKRFLVTMDVTF 361
F ++ P Q + ++ +F+GY GY+ D K+ + + DV F
Sbjct: 656 FAHV------PKEQRTKLD-DKSIPCIFIGYGDEEFGYRLWDPVKKKVIRSRDVVF 704
>M300_ARATH (P93293) Hypothetical mitochondrial protein AtMg00300
(ORF145a) (ORF1451)
Length = 145
Score = 55.5 bits (132), Expect = 8e-07
Identities = 34/112 (30%), Positives = 52/112 (46%), Gaps = 12/112 (10%)
Query: 33 KTIVSAKKNGGLYYLEDELETGHQLGQISSFSESFFVSNNKDDVMLWHLRLGHPSFKYLK 92
+TI+ ++ LY L+ +ETG ES KD+ LWH RL H S + ++
Sbjct: 36 RTILKGNRHDSLYILQGSVETG----------ESNLAETAKDETRLWHSRLAHMSQRGME 85
Query: 93 IVFPKLFF-GHDFSSFQ-CEIYEFSKQHRSSFPVQTYKPSKLFSIIHSDVWG 142
++ K F SS + CE + K HR +F + +HSD+WG
Sbjct: 86 LLVKKGFLDSSKVSSLKFCEDCIYGKTHRVNFSTGQHTTKNPLDYVHSDLWG 137
>COPI_DROME (P04146) Copia protein (Gag-int-pol protein) [Contains:
Copia VLP protein; Copia protease (EC 3.4.23.-)]
Length = 1409
Score = 51.6 bits (122), Expect = 1e-05
Identities = 98/407 (24%), Positives = 161/407 (39%), Gaps = 63/407 (15%)
Query: 70 SNNKDDVMLWHLRLGHPSF-KYLKIVFPKLFFGHDFSS---FQCEIYE---FSKQHRSSF 122
+ +K++ LWH R GH S K L+I +F + CEI E KQ R F
Sbjct: 409 AKHKNNFRLWHERFGHISDGKLLEIKRKNMFSDQSLLNNLELSCEICEPCLNGKQARLPF 468
Query: 123 PV---QTYKPSKLFSIIHSDVWGPNRINSYPTKDGLSPLLMIILDFIGYI--Y*KKNQR* 177
+T+ LF ++HSDV GP + T D + ++ + F Y Y K +
Sbjct: 469 KQLKDKTHIKRPLF-VVHSDVCGPI---TPVTLDDKNYFVIFVDQFTHYCVTYLIKYKS- 523
Query: 178 DKLSR----ILLN*CRLSLIPLYKFSELTMEL--NILTQF*ETFFFMENGIVQQSTCVSS 231
D S + + +L +Y + + E N + QF ++ GI T +
Sbjct: 524 DVFSMFQDFVAKSEAHFNLKVVYLYIDNGREYLSNEMRQF-----CVKKGISYHLTVPHT 578
Query: 232 PQQNGITERKNRHLLEMARALLFFH*SSKILMG*GCINCCTL-----NKLYVISCFKP*D 286
PQ NG++ER R + E AR ++ K G + L ++ V S P +
Sbjct: 579 PQLNGVSERMIRTITEKARTMVSGAKLDKSFWGEAVLTATYLINRIPSRALVDSSKTPYE 638
Query: 287 SSRNLLKILSKCPYFSRFAFKNIRMHYFCP*TQTNRQT*--TRASKRVFVGYSPTRKGYK 344
N L F + +I+ N+Q ++ K +FVGY P G+K
Sbjct: 639 MWHNKKPYLKHLRVFGATVYVHIK----------NKQGKFDDKSFKSIFVGYEP--NGFK 686
Query: 345 CLDLNSKRFLVTMDVTFF*K*TFFFRTIIFKGGNQMKIHLIFFEDLILFENMFMSHSSRP 404
D +++F+V DV +K +F +D EN + SR
Sbjct: 687 LWDAVNEKFIVARDVV--------VDETNMVNSRAVKFETVFLKDSKESENKNFPNDSRK 738
Query: 405 FVSKENAPDNVSEHTPSMSEDVTKLVATNQNSNNDSLEPNDNQELIQ 451
+ E + S D + + ++ S N + PND++++IQ
Sbjct: 739 IIQTEFPNE-------SKECDNIQFLKDSKESENKNF-PNDSRKIIQ 777
>DP2S_METTH (O27456) DNA polymerase II small subunit (EC 2.7.7.7)
(Pol II)
Length = 482
Score = 39.7 bits (91), Expect = 0.044
Identities = 30/106 (28%), Positives = 46/106 (43%), Gaps = 3/106 (2%)
Query: 379 QMKIHLIFFEDLILFENMFMSHSSRPFVSKENAPDNVSEHTPSMSEDVTKLVATNQNSNN 438
+M + F D+ + F+ + FV N D SE S++ DV LV +
Sbjct: 219 EMDFSVAFISDVHIGSQTFLEDAFMKFVKWING-DFGSEEQRSLAADVKYLVVAGDIVDG 277
Query: 439 DSLEPNDNQELIQMSLHEHPYNETERKFGEVEGTWKGIIYGRRNHD 484
+ P +EL+ +HE Y E R FG++ K I+ NHD
Sbjct: 278 IGIYPGQEKELLIRDIHEQ-YEEAARLFGDIRSDIK-IVMIPGNHD 321
>SCA4_RICAF (Q9AJ83) Antigenic heat-stable 120 kDa protein (PS120)
(120 kDa antigen) (Protein PS 120) (Fragment)
Length = 1011
Score = 37.0 bits (84), Expect = 0.29
Identities = 35/139 (25%), Positives = 56/139 (40%), Gaps = 20/139 (14%)
Query: 398 MSHSSRPFVSKENAPDNVSEHTPSMSEDVTKLVATNQNSNNDSLEPNDNQELIQMSLHE- 456
+ S P VS E D + + T S+ +++KL N+ L PN N + ++ E
Sbjct: 825 LEKSKEPVVSAE---DRIVQETSSILNNISKLAVEKVNNLRAMLSPNGNLKTLEEKKEES 881
Query: 457 -HPYNETERKFGEVEGTWKGIIYGRRN--HDKVVE--------DLIPQHSHESEPRENQP 505
+E + FG T + + + N DK + D + Q +E EN
Sbjct: 882 IKKVDELVKAFGTKSSTEEQQSFIKTNLIDDKTLSKEVRLQTIDKLLQEQKRAEAIENPS 941
Query: 506 IKTN-----KGKGKISPFS 519
+KT GK K+ P S
Sbjct: 942 VKTEDVRVVSGKSKLKPIS 960
>SCA4_RICCN (Q52658) Antigenic heat-stable 120 kDa protein (PS120)
(120 kDa antigen) (Protein PS 120) (RCA)
Length = 1022
Score = 36.6 bits (83), Expect = 0.37
Identities = 35/139 (25%), Positives = 56/139 (40%), Gaps = 20/139 (14%)
Query: 398 MSHSSRPFVSKENAPDNVSEHTPSMSEDVTKLVATNQNSNNDSLEPNDNQELIQMSLHE- 456
+ S P VS E + + + T S+ +++KL N+ L PN N + ++ E
Sbjct: 830 LEKSKEPVVSAE---ERIVQETSSILNNISKLAVEKVNNFRAMLSPNGNLKTLEEKKEEA 886
Query: 457 -HPYNETERKFGEVEGTWKGIIYGRRN--HDKVVE--------DLIPQHSHESEPRENQP 505
+E + FG T + + + N DK + D + Q SE EN
Sbjct: 887 IKKVDELVKAFGTKSSTEEQQSFIKTNLIDDKTLSKEVRLQTIDKLLQEQKRSEAIENPS 946
Query: 506 IKTN-----KGKGKISPFS 519
+KT GK K+ P S
Sbjct: 947 VKTEDVRVVSGKSKLKPIS 965
>SCA4_RICMN (Q9AJ82) Antigenic heat-stable 120 kDa protein (PS120)
(120 kDa antigen) (Protein PS 120) (Fragment)
Length = 1011
Score = 35.4 bits (80), Expect = 0.83
Identities = 34/139 (24%), Positives = 56/139 (39%), Gaps = 20/139 (14%)
Query: 398 MSHSSRPFVSKENAPDNVSEHTPSMSEDVTKLVATNQNSNNDSLEPNDNQELIQMSLHE- 456
+ S P VS E + + + T S+ +++KL N+ L PN N + ++ E
Sbjct: 825 LEKSKEPVVSAE---ERIVQETSSILNNISKLAVEKVNNFRAMLSPNGNLKTLEEKKEES 881
Query: 457 -HPYNETERKFGEVEGTWKGIIYGRRN--HDKVVE--------DLIPQHSHESEPRENQP 505
+E + FG T + + + N DK + D + Q +E EN
Sbjct: 882 IKKVDELVKAFGTKSSTEEQQSFIKTNLIDDKTLSKEVRLQTIDKLLQEQKRAEAIENPS 941
Query: 506 IKTN-----KGKGKISPFS 519
+KT GK K+ P S
Sbjct: 942 VKTEDVRVVSGKSKLKPIS 960
>SCA4_RICJA (Q9AJ79) Antigenic heat-stable 120 kDa protein (PS120)
(120 kDa antigen) (Protein PS 120) (rps120)
Length = 1018
Score = 35.4 bits (80), Expect = 0.83
Identities = 34/139 (24%), Positives = 56/139 (39%), Gaps = 20/139 (14%)
Query: 398 MSHSSRPFVSKENAPDNVSEHTPSMSEDVTKLVATNQNSNNDSLEPNDNQELIQMSLHE- 456
+ S P VS E + + + T S+ +++KL N+ L PN N + ++ E
Sbjct: 826 LEKSKEPVVSAE---ERIVQETSSILNNISKLAVEKVNNFRAMLSPNGNLKTLEEKKEES 882
Query: 457 -HPYNETERKFGEVEGTWKGIIYGRRN--HDKVVE--------DLIPQHSHESEPRENQP 505
+E + FG T + + + N DK + D + Q +E EN
Sbjct: 883 IKKVDELVKAFGTKSSTEEQQSFIKANLIDDKTLSKEIRLQTIDKLLQEQKRAEAIENPS 942
Query: 506 IKTN-----KGKGKISPFS 519
+KT GK K+ P S
Sbjct: 943 VKTEDVRVVSGKSKLKPIS 961
>SCA4_RICPA (Q9AJ75) Antigenic heat-stable 120 kDa protein (PS120)
(120 kDa antigen) (Protein PS 120) (Fragment)
Length = 1010
Score = 35.0 bits (79), Expect = 1.1
Identities = 34/139 (24%), Positives = 55/139 (39%), Gaps = 20/139 (14%)
Query: 398 MSHSSRPFVSKENAPDNVSEHTPSMSEDVTKLVATNQNSNNDSLEPNDNQELIQMSLHE- 456
+ S P VS E + + + T S+ +++KL N+ L PN N + ++ E
Sbjct: 824 LEKSKEPVVSAE---ERIVQETSSILNNISKLAVEKVNNLRSMLSPNGNLKTLEEKKEES 880
Query: 457 -HPYNETERKFGEVEGTWKGIIYGRRN--HDKVVE--------DLIPQHSHESEPRENQP 505
+E + FG T + + + N DK + D + Q +E EN
Sbjct: 881 IKKVDELVKAFGTKSSTEEQQSFIKTNLIDDKTLSKEVRLQTIDKLLQEQKRAEAIENPS 940
Query: 506 IKTN-----KGKGKISPFS 519
KT GK K+ P S
Sbjct: 941 FKTEDVRVVSGKSKLKPIS 959
>SCA4_RICSI (Q9AJ77) Antigenic heat-stable 120 kDa protein (PS120)
(120 kDa antigen) (Protein PS 120) (Fragment)
Length = 991
Score = 33.5 bits (75), Expect = 3.2
Identities = 34/139 (24%), Positives = 54/139 (38%), Gaps = 20/139 (14%)
Query: 398 MSHSSRPFVSKENAPDNVSEHTPSMSEDVTKLVATNQNSNNDSLEPNDNQELIQMSLHE- 456
+ S P VS E + + T S+ +++KL N+ L PN N + + E
Sbjct: 805 LEKSKEPVVSAE---ERIVHETSSILNNISKLAVEKVNNFRAMLSPNGNLKTLAEKKEES 861
Query: 457 -HPYNETERKFGEVEGTWKGIIYGRRN--HDKVVE--------DLIPQHSHESEPRENQP 505
+E + FG T + + + N DK + D + Q +E EN
Sbjct: 862 IKKVDELVKAFGTKSSTEEQQSFIKTNLIDDKTLSKEVRLQTIDKLLQEQKRAEAIENPS 921
Query: 506 IKTN-----KGKGKISPFS 519
+KT GK K+ P S
Sbjct: 922 VKTEDVRVVSGKSKLKPIS 940
>YEAB_SCHPO (O14076) Hypothetical protein UNK4.11c in chromosome I
Length = 188
Score = 32.0 bits (71), Expect = 9.2
Identities = 38/138 (27%), Positives = 60/138 (42%), Gaps = 31/138 (22%)
Query: 391 ILFENMFMSHSSRP----------FVSKENAPDNVSEHTPSMSEDV----TKLVATNQNS 436
++ EN SHS+ P +KE +NV E S +E+V TK+ T++ +
Sbjct: 62 LIEENDLESHSNEPNLVQGRASFGLFNKELGEENVDEKDVSKNEEVDVNGTKITDTSELT 121
Query: 437 NNDSLEPNDNQELIQMSLHEHPYNETERKFGEVEGTWKGIIYGRRNHDKVVEDLIPQHSH 496
+ QEL+ E RK EV+ K +R +++ +D+I HS
Sbjct: 122 ERE----RRKQELVSKKA------EASRKM-EVKAPAKE--SKKRKVNELSQDVISLHS- 167
Query: 497 ESEPRENQPIKTNKGKGK 514
P+E+ KT K K K
Sbjct: 168 ---PKESNARKTKKNKNK 182
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.355 0.158 0.554
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 105,433,012
Number of Sequences: 164201
Number of extensions: 3962054
Number of successful extensions: 18370
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 18352
Number of HSP's gapped (non-prelim): 18
length of query: 1075
length of database: 59,974,054
effective HSP length: 121
effective length of query: 954
effective length of database: 40,105,733
effective search space: 38260869282
effective search space used: 38260869282
T: 11
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.6 bits)
S2: 71 (32.0 bits)
Medicago: description of AC148227.2