BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146752.2 + phase: 0
(394 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PPQ1_YEAST (P32945) Serine/threonine protein phosphatase PPQ (EC... 40 0.008
APAH_WIGBR (Q8D3I0) Bis(5'-nucleosyl)-tetraphosphatase, symmetri... 40 0.010
YF41_METJA (Q58936) Hypothetical protein MJ1541 39 0.030
PGCA_RAT (P07897) Aggrecan core protein precursor (Cartilage-spe... 37 0.11
PPZ_SCHPO (P78968) Serine/threonine protein phosphatase PP-Z (EC... 36 0.19
LECT_SOLTU (Q9S8M0) Chitin-binding lectin 1 precursor (PL-I) 35 0.25
YM2A_CAEEL (P34447) Hypothetical protein F54F2.2 in chromosome I... 35 0.43
RDGC_DROME (P40421) Serine/threonine protein phosphatase rdgC (E... 35 0.43
PRP2_ECOLI (P55799) Serine/threonine protein phosphatase 2 (EC 3... 35 0.43
PGCA_HUMAN (P16112) Aggrecan core protein precursor (Cartilage-s... 35 0.43
YSD1_CAEEL (P48459) Putative serine/threonine protein phosphatas... 34 0.57
YNV7_YEAST (P40152) Hypothetical 37.2 kDa protein in ALG9-RAP1 i... 34 0.57
PP14_ARATH (P48484) Serine/threonine protein phosphatase PP1 iso... 34 0.57
VCIP_RAT (Q8CF97) Deubiquitinating protein VCIP135 (EC 3.4.22.-)... 34 0.74
PGCA_BOVIN (P13608) Aggrecan core protein precursor (Cartilage-s... 34 0.74
VCIP_MOUSE (Q8CDG3) Deubiquitinating protein VCIP135 (EC 3.4.22.... 33 0.97
PP1B_DROME (P48462) Serine/threonine protein phosphatase beta is... 33 0.97
P2B2_YEAST (P14747) Serine/threonine protein phosphatase 2B cata... 33 0.97
ABF1_MOUSE (Q61329) Alpha-fetoprotein enhancer binding protein (... 33 0.97
DD37_HUMAN (Q8IY37) Probable ATP-dependent helicase DHX37 (DEAH-... 33 1.3
>PPQ1_YEAST (P32945) Serine/threonine protein phosphatase PPQ (EC
3.1.3.16)
Length = 549
Score = 40.4 bits (93), Expect = 0.008
Identities = 28/98 (28%), Positives = 46/98 (46%), Gaps = 11/98 (11%)
Query: 48 PTRLPSPSRLIAIGDLHGDLKKSKEALSIAGLIDSSGNYTGGSATVVQIGDVLDRGGDEI 107
PT L + + +GD+HG L ++G + S NY + +GD +DRG + +
Sbjct: 287 PTLLRLQAPIKVVGDVHGQFNDLLRILKLSG-VPSDTNY-------LFLGDYVDRGKNSL 338
Query: 108 KILYLLEKLKRQAAIHGGNFITMNGNHEIMNAEGDFRF 145
+ + LL K + + NF + GNHE N + F
Sbjct: 339 ETILLLLCYKIK---YKDNFFMLRGNHESANVTKMYGF 373
>APAH_WIGBR (Q8D3I0) Bis(5'-nucleosyl)-tetraphosphatase, symmetrical
(EC 3.6.1.41) (Diadenosine tetraphosphatase) (Ap4A
hydrolase) (Diadenosine 5',5'''-P1,P4-tetraphosphate
pyrophosphohydrolase)
Length = 272
Score = 40.0 bits (92), Expect = 0.010
Identities = 26/77 (33%), Positives = 37/77 (47%), Gaps = 14/77 (18%)
Query: 60 IGDLHGDLKKSKEALSIAGLIDSSGNYTGGSATVVQIGDVLDRGGDEIKILYLLEKLKRQ 119
IGD+HG + K L + N+ + + GD + RG D +K+L L+ KLKR
Sbjct: 6 IGDIHGCYSEFKSMLDLI-------NFNLKNDIIWIAGDFIGRGPDSLKVLRLIYKLKR- 57
Query: 120 AAIHGGNFITMNGNHEI 136
N + GNHEI
Sbjct: 58 ------NIFVVLGNHEI 68
>YF41_METJA (Q58936) Hypothetical protein MJ1541
Length = 420
Score = 38.5 bits (88), Expect = 0.030
Identities = 24/76 (31%), Positives = 38/76 (49%), Gaps = 4/76 (5%)
Query: 110 LYLLEKLKRQAAIHGGNFITMNGNHEIMNAEGDFRFATKNGVEEFKVWLEWFRQGNKMKN 169
L L E++K A +H G +N N ++ AE F FATKNG + + R+G
Sbjct: 300 LNLFEEIKVSAILHKG----VNLNPTVVKAEEAFNFATKNGAKALNIKAGEIREGYLADI 355
Query: 170 LCKGLEETVVDPLENV 185
+ L++ + P EN+
Sbjct: 356 VLINLDKPYLYPKENI 371
>PGCA_RAT (P07897) Aggrecan core protein precursor
(Cartilage-specific proteoglycan core protein) (CSPCP)
Length = 2124
Score = 36.6 bits (83), Expect = 0.11
Identities = 26/67 (38%), Positives = 32/67 (46%), Gaps = 4/67 (5%)
Query: 38 PPPPSSPPPIPTRLPSPSRLIAIGDLHGDLKKSKEALSIAGLIDSSGNYTGGSATVVQIG 97
P PS P T LPS DL GD S + +G +DSSG +GGS + + G
Sbjct: 790 PYTPSLAVPSGTELPSSGDTSGAPDLSGDFTGSTDT---SGRLDSSGEPSGGSESGLPSG 846
Query: 98 DVLDRGG 104
D LD G
Sbjct: 847 D-LDSSG 852
>PPZ_SCHPO (P78968) Serine/threonine protein phosphatase PP-Z (EC
3.1.3.16)
Length = 515
Score = 35.8 bits (81), Expect = 0.19
Identities = 27/91 (29%), Positives = 40/91 (43%), Gaps = 11/91 (12%)
Query: 48 PTRLPSPSRLIAIGDLHGDLKKSKEALSIAGLIDSSGNYTGGSATVVQIGDVLDRGGDEI 107
PT L + +GD+HG + G SS NY + +GD +DRG +
Sbjct: 234 PTLLELTPPVKIVGDVHGQYSDLIRLFEMCGFPPSS-NY-------LFLGDYVDRGKQSL 285
Query: 108 KILYLLEKLKRQAAIHGGNFITMNGNHEIMN 138
+ + LL K + + NF + GNHE N
Sbjct: 286 ETILLLFLYKIR---YPENFFLLRGNHECAN 313
>LECT_SOLTU (Q9S8M0) Chitin-binding lectin 1 precursor (PL-I)
Length = 323
Score = 35.4 bits (80), Expect = 0.25
Identities = 21/49 (42%), Positives = 25/49 (50%), Gaps = 2/49 (4%)
Query: 38 PPPPSSPPPIPTRLPSPSRLIAIGDLHGDLKKSKEALSIAGLIDSSGNY 86
PPPP+SPPP P LP P I G G K+ E SI G ++ Y
Sbjct: 195 PPPPASPPPPPPALPYPQCGIKKGG--GKCIKTGECCSIWGWCGTTNAY 241
Score = 31.2 bits (69), Expect = 4.8
Identities = 12/18 (66%), Positives = 12/18 (66%)
Query: 38 PPPPSSPPPIPTRLPSPS 55
PPPPS PPP P P PS
Sbjct: 156 PPPPSPPPPSPPSPPPPS 173
Score = 30.8 bits (68), Expect = 6.3
Identities = 12/18 (66%), Positives = 12/18 (66%)
Query: 38 PPPPSSPPPIPTRLPSPS 55
PPPPS PPP P P PS
Sbjct: 169 PPPPSPPPPPPPSPPPPS 186
Score = 30.4 bits (67), Expect = 8.2
Identities = 11/18 (61%), Positives = 13/18 (72%)
Query: 38 PPPPSSPPPIPTRLPSPS 55
PPPPS PPP P+ P P+
Sbjct: 182 PPPPSPPPPSPSPPPPPA 199
>YM2A_CAEEL (P34447) Hypothetical protein F54F2.2 in chromosome III,
isoform a
Length = 867
Score = 34.7 bits (78), Expect = 0.43
Identities = 28/96 (29%), Positives = 48/96 (49%), Gaps = 8/96 (8%)
Query: 30 SVSGGLFLPPPPSSPPPIPTRLPSPSRLIAIGDLHGDLKKSKEALSIAG-LIDSSGNYTG 88
+V+ G+F+PPP + PP+ T SR D L +K +LS +G LI S+ + +
Sbjct: 269 TVNSGIFVPPPTAFSPPLTT----SSRSSVAQDPSPPLTINKNSLSSSGPLIPSTAHLSA 324
Query: 89 GSATVVQI---GDVLDRGGDEIKILYLLEKLKRQAA 121
+A+ I G L + + L++L+ Q+A
Sbjct: 325 TTASATPIMANGSTLPPSSETTVGTHCLQQLQIQSA 360
>RDGC_DROME (P40421) Serine/threonine protein phosphatase rdgC (EC
3.1.3.16) (Retinal degeneration C protein)
Length = 661
Score = 34.7 bits (78), Expect = 0.43
Identities = 33/98 (33%), Positives = 41/98 (41%), Gaps = 12/98 (12%)
Query: 48 PTRLPSPSRLIAIGDLHGDLKKSKEALSIAGLIDSSGNYTGGSATVVQIGDVLDRGGDEI 107
P ++ GDLHG L L GL SS Y V GD +DRG +
Sbjct: 144 PVSTAVSQQVTVCGDLHGKLDDLLVVLHKNGLPSSSNPY-------VFNGDFVDRGKRGL 196
Query: 108 KILYLLEKLKRQAAIHGGNFITMNGNHE--IMNAEGDF 143
++L LL L A F+ GNHE +MNA F
Sbjct: 197 EVLLLL--LSLYLAFPNAVFLN-RGNHEDSVMNARYGF 231
>PRP2_ECOLI (P55799) Serine/threonine protein phosphatase 2 (EC
3.1.3.16)
Length = 218
Score = 34.7 bits (78), Expect = 0.43
Identities = 31/109 (28%), Positives = 48/109 (43%), Gaps = 33/109 (30%)
Query: 60 IGDLHGD---LKKSKEALSIAGLIDSSGNYTGGSATVVQIGDVLDRGGDEIKILYLLEKL 116
+GD+HG+ L+ LS ID ++ +GD +DRG + + +L LL +
Sbjct: 20 VGDIHGEYQLLQSRLHQLSFFPKID----------LLISVGDNIDRGPESLDVLRLLNQ- 68
Query: 117 KRQAAIHGGNFITMNGNHEIMNAEGDFRFATKNGVEEFKVWL----EWF 161
F ++ GNHE M E F T +G +WL +WF
Sbjct: 69 --------PWFTSVKGNHEAMALEA---FETGDG----NMWLASGGDWF 102
>PGCA_HUMAN (P16112) Aggrecan core protein precursor
(Cartilage-specific proteoglycan core protein) (CSPCP)
(Chondroitin sulfate proteoglycan core protein 1)
Length = 2415
Score = 34.7 bits (78), Expect = 0.43
Identities = 24/67 (35%), Positives = 32/67 (46%), Gaps = 4/67 (5%)
Query: 38 PPPPSSPPPIPTRLPSPSRLIAIGDLHGDLKKSKEALSIAGLIDSSGNYTGGSATVVQIG 97
P PS P P T LPS D+ GD S + ++G +D SG +G A+ + G
Sbjct: 840 PYTPSPPEPSWTELPSSGEESGAPDVSGDFTGSGD---VSGHLDFSGQLSGDRASGLPSG 896
Query: 98 DVLDRGG 104
D LD G
Sbjct: 897 D-LDSSG 902
>YSD1_CAEEL (P48459) Putative serine/threonine protein phosphatase
C23G10.1 in chromosome II (EC 3.1.3.16)
Length = 354
Score = 34.3 bits (77), Expect = 0.57
Identities = 23/86 (26%), Positives = 39/86 (44%), Gaps = 11/86 (12%)
Query: 61 GDLHGDLKKSKEALS-IAGLIDSSGNYTGGSATVVQIGDVLDRGGDEIKILYLLEKLKRQ 119
GDLHG + + G NY + +GD +DRG ++++ L K
Sbjct: 93 GDLHGQYPDLIRLFAQVLGGFPPDSNY-------LFLGDYVDRGSFNLEVILLCLAYK-- 143
Query: 120 AAIHGGNFITMNGNHEIMNAEGDFRF 145
A + NF+ + GNHE+++ + F
Sbjct: 144 -ARYPNNFMMLRGNHEVIHINEKYGF 168
>YNV7_YEAST (P40152) Hypothetical 37.2 kDa protein in ALG9-RAP1
intergenic region
Length = 326
Score = 34.3 bits (77), Expect = 0.57
Identities = 31/108 (28%), Positives = 50/108 (45%), Gaps = 21/108 (19%)
Query: 37 LPPPPSSPPPIPT--RLPSPSRL----IAIGDLHGDLKKSKEALSIAGLIDSSGNYTGGS 90
L PP S P I T ++ + L + +GD+HG+ + E LID G +
Sbjct: 37 LSPPVSLLPTISTLKKIEHVTDLNKEYVFVGDVHGNYDEFIE------LIDDKIGGLGEN 90
Query: 91 ATVVQIGDVLDRGGDEIKIL-YLLEKLKRQAAIHGGNFITMNGNHEIM 137
T++ +GD + +G D K++ Y+L H + GNHEI+
Sbjct: 91 ITMILLGDFIHKGPDSDKVVSYILN--------HKDQVKCVLGNHEIL 130
>PP14_ARATH (P48484) Serine/threonine protein phosphatase PP1
isozyme 4 (EC 3.1.3.16)
Length = 321
Score = 34.3 bits (77), Expect = 0.57
Identities = 25/88 (28%), Positives = 37/88 (41%), Gaps = 11/88 (12%)
Query: 48 PTRLPSPSRLIAIGDLHGDLKKSKEALSIAGLIDSSGNYTGGSATVVQIGDVLDRGGDEI 107
P L + + GD+HG G S NY + +GD +DRG +
Sbjct: 60 PNLLELEAPIKICGDIHGQYSDLLRLFEYGGF-PPSANY-------LFLGDYVDRGKQSL 111
Query: 108 KILYLLEKLKRQAAIHGGNFITMNGNHE 135
+ + LL K + + GNF + GNHE
Sbjct: 112 ETICLLLAYKIK---YPGNFFLLRGNHE 136
>VCIP_RAT (Q8CF97) Deubiquitinating protein VCIP135 (EC 3.4.22.-)
(Valosin-containing protein p97/p47 complex-interacting
protein p135)
Length = 1221
Score = 33.9 bits (76), Expect = 0.74
Identities = 15/41 (36%), Positives = 20/41 (48%)
Query: 38 PPPPSSPPPIPTRLPSPSRLIAIGDLHGDLKKSKEALSIAG 78
PPPP PPP P P S +A G L K ++ ++G
Sbjct: 6 PPPPLPPPPPPPEAPQTSSSLAAAATPGGLSKRRDRRILSG 46
>PGCA_BOVIN (P13608) Aggrecan core protein precursor
(Cartilage-specific proteoglycan core protein) (CSPCP)
Length = 2364
Score = 33.9 bits (76), Expect = 0.74
Identities = 24/67 (35%), Positives = 34/67 (49%), Gaps = 4/67 (5%)
Query: 38 PPPPSSPPPIPTRLPSPSRLIAIGDLHGDLKKSKEALSIAGLIDSSGNYTGGSATVVQIG 97
P S+P P T LPS + + ++ GD S E I+G +D SG +G SA+ +
Sbjct: 902 PSTLSAPVPSRTELPSSGEVSGVPEISGDFTGSGE---ISGHLDFSGQPSGESASGLPSE 958
Query: 98 DVLDRGG 104
D LD G
Sbjct: 959 D-LDSSG 964
>VCIP_MOUSE (Q8CDG3) Deubiquitinating protein VCIP135 (EC
3.4.22.-) (Valosin-containing protein p97/p47
complex-interacting protein p135)
Length = 1220
Score = 33.5 bits (75), Expect = 0.97
Identities = 15/41 (36%), Positives = 20/41 (48%)
Query: 38 PPPPSSPPPIPTRLPSPSRLIAIGDLHGDLKKSKEALSIAG 78
PPPP PPP P P S +A G L K ++ ++G
Sbjct: 6 PPPPLPPPPPPPEAPQTSSSLAAAASPGGLSKRRDRRILSG 46
>PP1B_DROME (P48462) Serine/threonine protein phosphatase beta
isoform (EC 3.1.3.16) (Flap wing protein)
Length = 330
Score = 33.5 bits (75), Expect = 0.97
Identities = 27/90 (30%), Positives = 39/90 (43%), Gaps = 13/90 (14%)
Query: 46 PIPTRLPSPSRLIAIGDLHGDLKKSKEALSIAGLIDSSGNYTGGSATVVQIGDVLDRGGD 105
PI L +P LI GD+HG G + NY + +GD +DRG
Sbjct: 49 PILLELEAP--LIICGDIHGQYTDLLRLFEYGGF-PPAANY-------LFLGDYVDRGKQ 98
Query: 106 EIKILYLLEKLKRQAAIHGGNFITMNGNHE 135
++ + LL K + + NF + GNHE
Sbjct: 99 SLETICLLLAYKIK---YPENFFLLRGNHE 125
>P2B2_YEAST (P14747) Serine/threonine protein phosphatase 2B
catalytic subunit A2 (EC 3.1.3.16) (Calcineurin A2)
(Calmodulin-binding protein 2)
Length = 604
Score = 33.5 bits (75), Expect = 0.97
Identities = 24/98 (24%), Positives = 38/98 (38%), Gaps = 11/98 (11%)
Query: 48 PTRLPSPSRLIAIGDLHGDLKKSKEALSIAGLIDSSGNYTGGSATVVQIGDVLDRGGDEI 107
P + P+ + GD+HG + + G + + + +GD +DRG
Sbjct: 130 PNLISVPAPITVCGDIHGQYFDLLKLFEVGG--------DPATTSYLFLGDYVDRGSFSF 181
Query: 108 KILYLLEKLKRQAAIHGGNFITMNGNHEIMNAEGDFRF 145
+ L L LK H F + GNHE + F F
Sbjct: 182 ECLIYLYSLKLNFNDH---FWLLRGNHECKHLTSYFTF 216
>ABF1_MOUSE (Q61329) Alpha-fetoprotein enhancer binding protein (AT
motif-binding factor) (AT-binding transcription factor 1)
Length = 3726
Score = 33.5 bits (75), Expect = 0.97
Identities = 13/23 (56%), Positives = 15/23 (64%)
Query: 38 PPPPSSPPPIPTRLPSPSRLIAI 60
PPPP PPP+PT P P+ AI
Sbjct: 2049 PPPPPPPPPLPTAPPQPASAPAI 2071
>DD37_HUMAN (Q8IY37) Probable ATP-dependent helicase DHX37 (DEAH-box
protein 37)
Length = 1157
Score = 33.1 bits (74), Expect = 1.3
Identities = 12/30 (40%), Positives = 19/30 (63%)
Query: 31 VSGGLFLPPPPSSPPPIPTRLPSPSRLIAI 60
V G+ +PPPP++ PP+P L P+ I +
Sbjct: 210 VPAGMTVPPPPAAAPPLPRALAKPAVFIPV 239
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.141 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,664,610
Number of Sequences: 164201
Number of extensions: 2430168
Number of successful extensions: 11928
Number of sequences better than 10.0: 82
Number of HSP's better than 10.0 without gapping: 32
Number of HSP's successfully gapped in prelim test: 54
Number of HSP's that attempted gapping in prelim test: 11179
Number of HSP's gapped (non-prelim): 526
length of query: 394
length of database: 59,974,054
effective HSP length: 112
effective length of query: 282
effective length of database: 41,583,542
effective search space: 11726558844
effective search space used: 11726558844
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 67 (30.4 bits)
Medicago: description of AC146752.2