BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146330.2 + phase: 0
(223 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PSB1_PETHY (O82531) Proteasome subunit beta type 1 (EC 3.4.25.1)... 417 e-116
PSB1_ARATH (P42742) Proteasome subunit beta type 1 (EC 3.4.25.1)... 400 e-111
PSB1_ORYSA (O64464) Proteasome subunit beta type 1 (EC 3.4.25.1)... 366 e-101
PS12_CARAU (Q9IB83) Proteasome subunit beta type 1-B (EC 3.4.25.... 201 1e-51
PS11_CARAU (Q9IB84) Proteasome subunit beta type 1-A (EC 3.4.25.... 201 1e-51
PSB1_HUMAN (P20618) Proteasome subunit beta type 1 (EC 3.4.25.1)... 198 1e-50
PSB1_MOUSE (O09061) Proteasome subunit beta type 1 (EC 3.4.25.1)... 196 3e-50
PSB1_RAT (P18421) Proteasome subunit beta type 1 (EC 3.4.25.1) (... 193 2e-49
PSB1_DROME (P40304) Proteasome subunit beta type 1 (EC 3.4.25.1)... 190 2e-48
PSB1_CAEEL (P34286) Proteasome subunit beta type 1 (EC 3.4.25.1)... 176 5e-44
PSB1_TRYBB (Q9U794) Proteasome subunit beta type 1 (EC 3.4.25.1)... 167 1e-41
PSB1_YEAST (P23724) Potential proteasome component C5 (EC 3.4.25... 164 2e-40
PSB1_SCHPO (Q9UQY2) Probable proteasome subunit beta type 1 (EC ... 154 2e-37
PSMB_PYRHO (O50110) Proteasome beta subunit precursor (EC 3.4.25... 91 2e-18
PSB3_GIALA (Q9N9W8) Proteasome subunit beta type 3 (EC 3.4.25.1) 89 6e-18
PSMB_PYRFU (Q8U125) Proteasome beta subunit precursor (EC 3.4.25... 87 2e-17
PSMB_PYRAB (Q9V0N9) Proteasome beta subunit precursor (EC 3.4.25... 86 5e-17
PSB3_SCHPO (Q9Y7T8) Probable proteasome subunit beta type 3 (EC ... 83 6e-16
PSMB_METTE (Q9P992) Proteasome beta subunit precursor (EC 3.4.25... 79 1e-14
PS31_ARATH (Q9XI05) Proteasome subunit beta type 3-1 (EC 3.4.25.... 78 2e-14
>PSB1_PETHY (O82531) Proteasome subunit beta type 1 (EC 3.4.25.1)
(20S proteasome alpha subunit F) (20S proteasome subunit
beta-6)
Length = 223
Score = 417 bits (1071), Expect = e-116
Identities = 203/223 (91%), Positives = 214/223 (95%)
Query: 1 MTKQHANWSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMAS 60
MTKQ ANWSPYDNNGG+CVA+AG+DYCVIAADTRMSTGYNILTRDYSKI LA+KCVMAS
Sbjct: 1 MTKQQANWSPYDNNGGTCVAVAGADYCVIAADTRMSTGYNILTRDYSKIIKLADKCVMAS 60
Query: 61 SGFQADVKALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLD 120
SGFQADV+ALQKVL++RHL YQHQHNKQMSCPAM QLLSNTLYYKRFFPYYSFNVLGGLD
Sbjct: 61 SGFQADVRALQKVLASRHLIYQHQHNKQMSCPAMGQLLSNTLYYKRFFPYYSFNVLGGLD 120
Query: 121 SEGKGCVFTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAE 180
SEGKGCVFTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAE
Sbjct: 121 SEGKGCVFTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAE 180
Query: 181 AVDLVKTVFASATERDIYTGDKVEIVILNAGGIHREFMDLRKD 223
A+DLVKT FASATERDIYTGD++EIVILNA GI RE M+LRKD
Sbjct: 181 AIDLVKTCFASATERDIYTGDRLEIVILNASGIRREEMELRKD 223
>PSB1_ARATH (P42742) Proteasome subunit beta type 1 (EC 3.4.25.1)
(20S proteasome alpha subunit F) (Proteasome component
C5) (TAS-F22/FAFP98)
Length = 223
Score = 400 bits (1029), Expect = e-111
Identities = 195/223 (87%), Positives = 209/223 (93%)
Query: 1 MTKQHANWSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMAS 60
MTKQHANWSPYDNNGG+CVAIAGSDYCVIAADTRMSTGY+IL+RDYSKI LA++ V++S
Sbjct: 1 MTKQHANWSPYDNNGGTCVAIAGSDYCVIAADTRMSTGYSILSRDYSKIHKLADRAVLSS 60
Query: 61 SGFQADVKALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLD 120
SGFQADVKALQKVL +RHL YQHQHNKQMSCPAMAQLLSNTLY+KRFFPYY+FNVLGGLD
Sbjct: 61 SGFQADVKALQKVLKSRHLIYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 120
Query: 121 SEGKGCVFTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAE 180
EGKGCVFTYDAVGSYERVGY +QGSGSTLIMPFLDNQLKSPSPLLLP QD+ TPLSEAE
Sbjct: 121 EEGKGCVFTYDAVGSYERVGYGAQGSGSTLIMPFLDNQLKSPSPLLLPKQDSNTPLSEAE 180
Query: 181 AVDLVKTVFASATERDIYTGDKVEIVILNAGGIHREFMDLRKD 223
AVDLVKTVFASATERDIYTGDK+EI+IL A GI E MDLRKD
Sbjct: 181 AVDLVKTVFASATERDIYTGDKLEIMILKADGIKTELMDLRKD 223
>PSB1_ORYSA (O64464) Proteasome subunit beta type 1 (EC 3.4.25.1)
(20S proteasome alpha subunit F) (20S proteasome subunit
beta-6)
Length = 221
Score = 366 bits (940), Expect = e-101
Identities = 175/213 (82%), Positives = 195/213 (91%)
Query: 11 YDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKAL 70
Y+NNGG+CVAIAG+DYCV+AADTR+S GYNILTRD+SKI LA+KC +ASSGFQ D+KAL
Sbjct: 9 YENNGGTCVAIAGADYCVVAADTRLSVGYNILTRDHSKICELADKCALASSGFQGDIKAL 68
Query: 71 QKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCVFTY 130
K L+AR L YQHQHNK+MSCPAMAQLLSNTLYYKRFFPYY+FNVLGGLDSEGKGCVFTY
Sbjct: 69 HKNLAARELLYQHQHNKRMSCPAMAQLLSNTLYYKRFFPYYAFNVLGGLDSEGKGCVFTY 128
Query: 131 DAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKTVFA 190
DAVGSYER GYS+QG+GS LIMP LDNQLKSPSPLLLPA+DAVTPLSE EAVDLVK VFA
Sbjct: 129 DAVGSYERTGYSAQGTGSALIMPVLDNQLKSPSPLLLPARDAVTPLSETEAVDLVKDVFA 188
Query: 191 SATERDIYTGDKVEIVILNAGGIHREFMDLRKD 223
SATERDIYTGDK+EIV++N G RE++DLRKD
Sbjct: 189 SATERDIYTGDKLEIVVINKAGTKREYIDLRKD 221
>PS12_CARAU (Q9IB83) Proteasome subunit beta type 1-B (EC 3.4.25.1)
(20S proteasome beta 6 subunit B) (B6-B)
Length = 237
Score = 201 bits (510), Expect = 1e-51
Identities = 100/216 (46%), Positives = 141/216 (64%), Gaps = 4/216 (1%)
Query: 8 WSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADV 67
+SPY NGG+ +A+AG D+ ++A+DTR+S GY+I +RD K L + V+ SGF D
Sbjct: 26 FSPYAFNGGTVLAVAGEDFALVASDTRLSEGYSIHSRDSPKCYKLTDTTVIGCSGFHGDC 85
Query: 68 KALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCV 127
L K++ AR Y+H +NK M+ A+A +LS LY +RFFPYY +N++GGLD EG+G V
Sbjct: 86 LTLTKIIEARLKMYKHSNNKSMTSGAIAAMLSTILYGRRFFPYYVYNIIGGLDEEGRGAV 145
Query: 128 FTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKT 187
+++D VGSY+R Y + GS S ++ P LDNQ+ + PLS+ +AV LVK
Sbjct: 146 YSFDPVGSYQRDTYKAGGSASAMLQPLLDNQIGFKN----MENVEQVPLSQEKAVQLVKD 201
Query: 188 VFASATERDIYTGDKVEIVILNAGGIHREFMDLRKD 223
VF SA ERD+YTGD ++I I+ GI E + LRKD
Sbjct: 202 VFISAAERDVYTGDALKICIITKEGIREEIVPLRKD 237
>PS11_CARAU (Q9IB84) Proteasome subunit beta type 1-A (EC 3.4.25.1)
(20S proteasome beta 6 subunit A) (B6-A)
Length = 238
Score = 201 bits (510), Expect = 1e-51
Identities = 100/216 (46%), Positives = 141/216 (64%), Gaps = 4/216 (1%)
Query: 8 WSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADV 67
+SPY NGG+ +A+AG D+ ++A+DTR+S GY+I +RD K L + V+ SGF D
Sbjct: 27 FSPYAFNGGTVLAVAGEDFALVASDTRLSEGYSIHSRDSPKCYKLTDTTVIGCSGFHGDC 86
Query: 68 KALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCV 127
L K++ AR Y+H +NK M+ A+A +LS LY +RFFPYY +N++GGLD EG+G V
Sbjct: 87 LTLTKIIEARLKMYKHSNNKSMTSGAIAAMLSTILYGRRFFPYYVYNIIGGLDEEGRGAV 146
Query: 128 FTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKT 187
+++D VGSY+R Y + GS S ++ P LDNQ+ + PLS+ +AV LVK
Sbjct: 147 YSFDPVGSYQRDTYKAGGSASAMLQPLLDNQIGFKN----MENVEQVPLSQEKAVQLVKD 202
Query: 188 VFASATERDIYTGDKVEIVILNAGGIHREFMDLRKD 223
VF SA ERD+YTGD ++I I+ GI E + LRKD
Sbjct: 203 VFISAAERDVYTGDALKICIITKEGIREEIVPLRKD 238
>PSB1_HUMAN (P20618) Proteasome subunit beta type 1 (EC 3.4.25.1)
(Proteasome component C5) (Macropain subunit C5)
(Multicatalytic endopeptidase complex subunit C5)
(Proteasome gamma chain)
Length = 241
Score = 198 bits (503), Expect = 1e-50
Identities = 101/216 (46%), Positives = 140/216 (64%), Gaps = 4/216 (1%)
Query: 8 WSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADV 67
+SPY NGG+ +AIAG D+ ++A+DTR+S G++I TRD K L +K V+ SGF D
Sbjct: 30 FSPYVFNGGTILAIAGEDFAIVASDTRLSEGFSIHTRDSPKCYKLTDKTVIGCSGFHGDC 89
Query: 68 KALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCV 127
L K++ AR Y+H +NK M+ A+A +LS LY +RFFPYY +N++GGLD EGKG V
Sbjct: 90 LTLTKIIEARLKMYKHSNNKAMTTGAIAAMLSTILYSRRFFPYYVYNIIGGLDEEGKGAV 149
Query: 128 FTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKT 187
+++D VGSY+R + + GS S ++ P LDNQ+ + + PLS A+ LVK
Sbjct: 150 YSFDPVGSYQRDSFKAGGSASAMLQPLLDNQVGFKNMQNVEH----VPLSLDRAMRLVKD 205
Query: 188 VFASATERDIYTGDKVEIVILNAGGIHREFMDLRKD 223
VF SA ERD+YTGD + I I+ GI E + LRKD
Sbjct: 206 VFISAAERDVYTGDALRICIVTKEGIREETVSLRKD 241
>PSB1_MOUSE (O09061) Proteasome subunit beta type 1 (EC 3.4.25.1)
(Proteasome component C5) (Macropain subunit C5)
(Multicatalytic endopeptidase complex subunit C5)
(Proteasome gamma chain)
Length = 240
Score = 196 bits (499), Expect = 3e-50
Identities = 100/216 (46%), Positives = 140/216 (64%), Gaps = 4/216 (1%)
Query: 8 WSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADV 67
+SPY NGG+ +AIAG D+ ++A+DTR+S G++I TRD K L +K V+ SGF D
Sbjct: 29 FSPYAFNGGTVLAIAGEDFSIVASDTRLSEGFSIHTRDSPKCYKLTDKTVIGCSGFHGDC 88
Query: 68 KALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCV 127
L K++ AR Y+H +NK M+ A+A +LS LY +RFFPYY +N++GGLD EGKG V
Sbjct: 89 LTLTKIIEARLKMYKHSNNKAMTTGAIAAMLSTILYSRRFFPYYVYNIIGGLDEEGKGAV 148
Query: 128 FTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKT 187
+++D VGSY+R + + GS S ++ P LDNQ+ + + PL+ A+ LVK
Sbjct: 149 YSFDPVGSYQRDSFKAGGSASAMLQPLLDNQVGFKNMQNVEH----VPLTLDRAMRLVKD 204
Query: 188 VFASATERDIYTGDKVEIVILNAGGIHREFMDLRKD 223
VF SA ERD+YTGD + I I+ GI E + LRKD
Sbjct: 205 VFISAAERDVYTGDALRICIVTKEGIREETVPLRKD 240
>PSB1_RAT (P18421) Proteasome subunit beta type 1 (EC 3.4.25.1)
(Proteasome component C5) (Macropain subunit C5)
(Multicatalytic endopeptidase complex subunit C5)
(Proteasome gamma chain)
Length = 240
Score = 193 bits (491), Expect = 2e-49
Identities = 99/216 (45%), Positives = 139/216 (63%), Gaps = 4/216 (1%)
Query: 8 WSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADV 67
+SPY NGG+ +AIAG D+ ++A+DTR+S G++I TRD K L +K V+ SGF D
Sbjct: 29 FSPYAFNGGTVLAIAGEDFSIVASDTRLSEGFSIHTRDSPKCYKLTDKTVIGCSGFHGDC 88
Query: 68 KALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCV 127
L K++ AR Y+H +NK M+ A+A +LS LY +RFFPYY +N++ GLD EGKG V
Sbjct: 89 LTLTKIIEARLKMYKHSNNKAMTTGAIAAMLSTILYSRRFFPYYVYNIIEGLDEEGKGAV 148
Query: 128 FTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKT 187
+++D VGSY+R + + GS S ++ P LDNQ+ + + PL+ A+ LVK
Sbjct: 149 YSFDPVGSYQRDSFKAGGSASAMLQPLLDNQVGFKNMQNVEH----VPLTLDRAMRLVKD 204
Query: 188 VFASATERDIYTGDKVEIVILNAGGIHREFMDLRKD 223
VF SA ERD+YTGD + I I+ GI E + LRKD
Sbjct: 205 VFISAAERDVYTGDALRICIVTKEGIREETVPLRKD 240
>PSB1_DROME (P40304) Proteasome subunit beta type 1 (EC 3.4.25.1)
(Proteasome 26 kDa subunit)
Length = 235
Score = 190 bits (483), Expect = 2e-48
Identities = 99/220 (45%), Positives = 141/220 (64%), Gaps = 2/220 (0%)
Query: 4 QHANWSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGF 63
+H ++SPY++NGGS VAIAG D+ VIAADTR+S+GYNI +R SK+ L+ + V+ S+G
Sbjct: 18 KHPDFSPYESNGGSIVAIAGDDFAVIAADTRLSSGYNIHSRTQSKLFKLSPQTVLGSAGC 77
Query: 64 QADVKALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEG 123
AD +L + R +Y+H H + M+ A+AQ+LS +Y +RFFPYY N+L G+D+EG
Sbjct: 78 WADTLSLTGSIKVRMQSYEHTHLRTMTTEAVAQMLSIAMYNRRFFPYYVSNILAGIDNEG 137
Query: 124 KGCVFTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVD 183
KG V++YD +G E+ Y + G+ TL+ P LDNQ+ + L A L++ AV
Sbjct: 138 KGVVYSYDPIGHCEKATYRAGGTAGTLLQPVLDNQIGHKNMNLEDADK--IKLTKERAVS 195
Query: 184 LVKTVFASATERDIYTGDKVEIVILNAGGIHREFMDLRKD 223
+ F SA ERDIYTGD V I I+ GI + LR+D
Sbjct: 196 VASDTFISAAERDIYTGDSVLINIITKDGIEVRTLTLRQD 235
>PSB1_CAEEL (P34286) Proteasome subunit beta type 1 (EC 3.4.25.1)
(Proteasome subunit beta 6)
Length = 258
Score = 176 bits (445), Expect = 5e-44
Identities = 94/222 (42%), Positives = 135/222 (60%), Gaps = 5/222 (2%)
Query: 4 QHANWSPYDNNGGSCVAIAGSDYCVIAADTRMSTG-YNILTRDYSKISHLAEKCVMASSG 62
+ W+PY GGS AI+G ++ ++A+DTRM+ NILTRD KI L + ++ +SG
Sbjct: 40 ERQRWNPYSMEGGSTCAISGENFAIVASDTRMTQNDINILTRDAEKIQILNDNIILTTSG 99
Query: 63 FQADVKALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSE 122
F DV L+KVL +R Y+ + MS A+LLS LYY+RFFPYY+ +L G+D
Sbjct: 100 FYGDVLQLKKVLQSRLHKYRFDYRSDMSVDLCAELLSRNLYYRRFFPYYTGAILAGIDEH 159
Query: 123 GKGCVFTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAV 182
GKG VF+YD +G ER+GYS+ G+ +I+PFLD Q+ + L L+ A+
Sbjct: 160 GKGAVFSYDPIGCIERLGYSASGAAEPMIIPFLDCQI---GHVTLSEGYERPELTLDRAI 216
Query: 183 DLVKTVFASATERDIYTGDKVEIVILNAG-GIHREFMDLRKD 223
L+K F A ER+I TGDK+ +VI AG + +F+ LR+D
Sbjct: 217 SLMKDSFRGAAEREISTGDKIHLVIAEAGKPVVVKFLPLRED 258
>PSB1_TRYBB (Q9U794) Proteasome subunit beta type 1 (EC 3.4.25.1)
(20S proteasome beta 6 subunit)
Length = 258
Score = 167 bits (424), Expect = 1e-41
Identities = 98/223 (43%), Positives = 135/223 (59%), Gaps = 14/223 (6%)
Query: 7 NWSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTR-DYSKISHLAEKCVMASSGFQA 65
+WSPY +NGG+ AIAGS+Y V+ ADTR++ + I TR D SK+ L ++ +ASSG QA
Sbjct: 44 HWSPYTDNGGTIAAIAGSNYVVLGADTRLNGDFCIHTRSDTSKLFKLTDRIFLASSGMQA 103
Query: 66 DVKALQKVLSARHLTYQHQHN-KQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGK 124
D LQ++L R YQ+ + K S A+A+L S LY +RFFPYY+FN++ G+D +G
Sbjct: 104 DRLQLQQMLKYRIQWYQYNNGGKVPSTKAIAKLTSTMLYQRRFFPYYTFNMIVGIDEKGA 163
Query: 125 GCVFTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVT----PLSEAE 180
G ++YD VGS E Y + GS S+ + P LD LL Q VT L+ E
Sbjct: 164 GVCYSYDPVGSTEPFRYGTCGSASSFVEPLLD--------CLLTRQHMVTQAPADLTMEE 215
Query: 181 AVDLVKTVFASATERDIYTGDKVEIVILNAGGIHREFMDLRKD 223
A+ ++K F A ERDI+TGD V I+ A G+ E +LRKD
Sbjct: 216 ALGMLKNAFTGAAERDIFTGDTVCFHIITADGVGTEMFELRKD 258
>PSB1_YEAST (P23724) Potential proteasome component C5 (EC 3.4.25.1)
(Multicatalytic endopeptidase complex subunit C5)
Length = 241
Score = 164 bits (414), Expect = 2e-40
Identities = 90/221 (40%), Positives = 132/221 (59%), Gaps = 5/221 (2%)
Query: 8 WSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADV 67
++PY +NGG+ + IAG D+ V+A DTR T Y+I +R K+ + VM+++GF AD
Sbjct: 21 FNPYGDNGGTILGIAGEDFAVLAGDTRNITDYSINSRYEPKVFDCGDNIVMSANGFAADG 80
Query: 68 KALQKVLSARHLTYQHQHN-KQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGC 126
AL K Y HN K++S + A+ + + LY KRFFPYY ++ GLD +GKG
Sbjct: 81 DALVKRFKNSVKWYHFDHNDKKLSINSAARNIQHLLYGKRFFPYYVHTIIAGLDEDGKGA 140
Query: 127 VFTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTP----LSEAEAV 182
V+++D VGSYER + G+ ++LIMPFLDNQ+ + V LS E +
Sbjct: 141 VYSFDPVGSYEREQCRAGGAAASLIMPFLDNQVNFKNQYEPGTNGKVKKPLKYLSVEEVI 200
Query: 183 DLVKTVFASATERDIYTGDKVEIVILNAGGIHREFMDLRKD 223
LV+ F SATER I GD +EI+I+ G+ +EF +L++D
Sbjct: 201 KLVRDSFTSATERHIQVGDGLEILIVTKDGVRKEFYELKRD 241
>PSB1_SCHPO (Q9UQY2) Probable proteasome subunit beta type 1 (EC
3.4.25.1)
Length = 225
Score = 154 bits (389), Expect = 2e-37
Identities = 83/222 (37%), Positives = 122/222 (54%), Gaps = 4/222 (1%)
Query: 6 ANWSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQA 65
+ + PY NGG+ VAIAG + ++A DTR GYNI TR ++ + + V+ +SGF+A
Sbjct: 4 SQFDPYVQNGGTTVAIAGDGFAILAGDTRSVNGYNINTRFQPRVHEVGDDLVIGASGFEA 63
Query: 66 DVKALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKG 125
D AL K + R Y H ++MS + A ++ LY KRFFPYY + + G+D EGKG
Sbjct: 64 DALALVKRIQQRIDLYHDNHERKMSAQSCACMVRTLLYGKRFFPYYVYTTVAGIDKEGKG 123
Query: 126 CVFTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTP----LSEAEA 181
++++D VGSYER + GS + I PFLDNQ+ + + + L EA
Sbjct: 124 EIYSFDPVGSYEREWCRAGGSAANFITPFLDNQVNLHNQYVPGSHGKERKPRRLLKLEEA 183
Query: 182 VDLVKTVFASATERDIYTGDKVEIVILNAGGIHREFMDLRKD 223
+ + F SA ER I GD V + I+ G+ + L+KD
Sbjct: 184 MKITTDAFTSAGERHIEVGDSVLVKIITKEGVETRIIPLKKD 225
>PSMB_PYRHO (O50110) Proteasome beta subunit precursor (EC 3.4.25.1)
(Multicatalytic endopeptidase complex beta subunit)
Length = 207
Score = 91.3 bits (225), Expect = 2e-18
Identities = 61/205 (29%), Positives = 101/205 (48%), Gaps = 16/205 (7%)
Query: 15 GGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKALQKVL 74
G + V I D V+AAD R S G I R+ +KI + E +A +G D+ L ++L
Sbjct: 10 GTTTVGIVCKDGVVLAADRRASLGNIIYARNVTKIHKIDEHLAIAGAGDVGDILNLVRLL 69
Query: 75 SARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCVFTYDAVG 134
A Y Q K+MS A+A LL+N + ++FPY ++ ++GG D + K +++ D VG
Sbjct: 70 RAEAKLYYSQSGKRMSVKALATLLANIMNGAKYFPYLAWFLVGGYDEKPK--LYSVDMVG 127
Query: 135 SYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKTVFASATE 194
Y++ GSG LD++ K L+ E + L +A +
Sbjct: 128 GITEDKYATAGSGMEFAYSILDSEYKD-------------NLTLEEGIKLAVKAINTAIK 174
Query: 195 RDIYTGDKVEIVILNAGGIHREFMD 219
RD+++GD + +V + G ++E D
Sbjct: 175 RDVFSGDGILVVTITKEG-YKELSD 198
>PSB3_GIALA (Q9N9W8) Proteasome subunit beta type 3 (EC 3.4.25.1)
Length = 207
Score = 89.4 bits (220), Expect = 6e-18
Identities = 52/204 (25%), Positives = 99/204 (48%), Gaps = 15/204 (7%)
Query: 11 YDNNGGSCVAIAGSDYCVIAADTRMSTGYNILT-RDYSKISHLAEKCVMASSGFQADVKA 69
++ NG + + I G D +AAD R+ Y LT + +I L++ C++ +G Q DV
Sbjct: 5 WEYNGSALIGIKGXDCVALAADKRLGCSYQTLTIGNEERIFRLSQSCLLGLAGLQTDVLT 64
Query: 70 LQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCVFT 129
+ + + Y Q ++++ A A+++S+ LY RF PY+ ++ G +++GK +
Sbjct: 65 TYRQIRMKSNLYHLQEDREIRPRATAKMISSFLYKHRFGPYFISPIIAGFEADGKPYLGA 124
Query: 130 YDAVGSYER-VGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKTV 188
D +G + + G+ S ++ + +K +SE E + L++ +
Sbjct: 125 MDTIGCLDDCTNFQIAGTASDFLLGLCETHVK-------------PDMSEDEILPLMEQI 171
Query: 189 FASATERDIYTGDKVEIVILNAGG 212
SAT RD Y+G +V+L G
Sbjct: 172 IRSATNRDAYSGWGAHVVVLRRDG 195
>PSMB_PYRFU (Q8U125) Proteasome beta subunit precursor (EC 3.4.25.1)
(Multicatalytic endopeptidase complex beta subunit)
Length = 206
Score = 87.4 bits (215), Expect = 2e-17
Identities = 56/198 (28%), Positives = 96/198 (48%), Gaps = 15/198 (7%)
Query: 15 GGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKALQKVL 74
G + V + + V+AADTR S G I ++ +KI + E +A +G D+ L ++L
Sbjct: 10 GTTTVGLVCKEGVVLAADTRASLGNIIYAKNVTKIHKIDEHLAIAGAGDVGDILNLVRLL 69
Query: 75 SARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCVFTYDAVG 134
A Y+ K+MS A+A LL+N L ++FPY + ++GG D + + +F+ D VG
Sbjct: 70 KAEANLYKSTVGKEMSVKALATLLANILNGSKYFPYLGWFLVGGYDEKPR--LFSVDMVG 127
Query: 135 SYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKTVFASATE 194
Y++ GSG LD++ + +S + + L A +
Sbjct: 128 GITEDNYAAAGSGMEFAYSILDSEYRE-------------EMSVNDGIKLAVKAINVAIK 174
Query: 195 RDIYTGDKVEIVILNAGG 212
RD++TGD + +V + G
Sbjct: 175 RDVFTGDGLLVVTITKDG 192
>PSMB_PYRAB (Q9V0N9) Proteasome beta subunit precursor (EC 3.4.25.1)
(Multicatalytic endopeptidase complex beta subunit)
Length = 207
Score = 86.3 bits (212), Expect = 5e-17
Identities = 59/207 (28%), Positives = 99/207 (47%), Gaps = 16/207 (7%)
Query: 15 GGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKALQKVL 74
G + V I SD V+AAD R S G + ++ +KI + E +A +G D+ L ++L
Sbjct: 10 GTTTVGIVCSDGVVLAADRRASLGNIVYAKNVTKIHKIDEHLAIAGAGDVGDILNLVRLL 69
Query: 75 SARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCVFTYDAVG 134
A Y Q K+MS A+A LL+N L R PY ++ ++GG D + + +++ D +G
Sbjct: 70 RAEAKLYYAQSGKRMSVKALATLLANMLNGARMLPYLAWFLVGGFDEKPR--LYSVDMMG 127
Query: 135 SYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKTVFASATE 194
Y + GSG LD++ + L E + + SA +
Sbjct: 128 GITEDKYVAAGSGMEFAYSVLDSEYRE-------------DLKVREGIKIAVEAINSAIK 174
Query: 195 RDIYTGDKVEIVILNAGGIHREFMDLR 221
RD+++GD + +V + G +RE + R
Sbjct: 175 RDVFSGDGIMVVTITEEG-YRELSNSR 200
>PSB3_SCHPO (Q9Y7T8) Probable proteasome subunit beta type 3 (EC
3.4.25.1)
Length = 204
Score = 82.8 bits (203), Expect = 6e-16
Identities = 40/121 (33%), Positives = 70/121 (57%)
Query: 14 NGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKALQKV 73
NGGSCVA+AG + IA+D R+ LT ++ K+ + +K + +G DV+ L ++
Sbjct: 7 NGGSCVAMAGKNCVAIASDLRLGVQSISLTNNFPKVFAMGDKTYLGLTGLATDVQTLYEL 66
Query: 74 LSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCVFTYDAV 133
+ Y+ + +Q+ A L+S+TLY KRF PY+SF V+ G+ ++ + +D++
Sbjct: 67 FRYKVNLYKFREERQIQPKTFANLVSSTLYEKRFGPYFSFPVVAGVSNDNTPFICGFDSI 126
Query: 134 G 134
G
Sbjct: 127 G 127
>PSMB_METTE (Q9P992) Proteasome beta subunit precursor (EC 3.4.25.1)
(Multicatalytic endopeptidase complex beta subunit)
Length = 210
Score = 78.6 bits (192), Expect = 1e-14
Identities = 49/202 (24%), Positives = 101/202 (49%), Gaps = 15/202 (7%)
Query: 15 GGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKALQKVL 74
G + V + +D V+A++ R + G+ I ++ K+ + + M ++G D + L +++
Sbjct: 9 GTTTVGVVCTDGIVLASEQRATMGHFIASKTAKKVYQIDDLVGMTTAGSVGDAQQLVRLV 68
Query: 75 SARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCVFTYDAV- 133
S Y+ + ++ M+ + L+SN L R++P ++GG+D G G +++ DA+
Sbjct: 69 SVESQLYKMRRDESMTIKGITTLMSNFLSRNRYYPMMVQLLIGGVDKNGPG-IYSLDAMG 127
Query: 134 GSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKTVFASAT 193
GS E S+ GSGS + L++Q + ++ E +DL +AT
Sbjct: 128 GSIEETRISATGSGSPMAYGVLEDQYRE-------------NMTVKEGLDLAIRAIHNAT 174
Query: 194 ERDIYTGDKVEIVILNAGGIHR 215
+RD +G+ +++V++ R
Sbjct: 175 KRDSASGENIDVVVITKEAFKR 196
>PS31_ARATH (Q9XI05) Proteasome subunit beta type 3-1 (EC 3.4.25.1)
(20S proteasome alpha subunit C1)
Length = 204
Score = 77.8 bits (190), Expect = 2e-14
Identities = 58/214 (27%), Positives = 95/214 (44%), Gaps = 14/214 (6%)
Query: 11 YDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKAL 70
++ NG + VA+ G + IA+D R+ + D+ +IS + ++ + SG DV+ L
Sbjct: 4 FEYNGSAVVAMVGKNCFAIASDRRLGVQLQTIATDFQRISKIHDRVFIGLSGLATDVQTL 63
Query: 71 QKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCVFTY 130
+ L RH YQ + + M A L+S LY KRF PY V+ GL + K + T
Sbjct: 64 YQRLVFRHKLYQLREERDMKPETFASLVSAILYEKRFGPYLCQPVIAGLGDDDKPFICTM 123
Query: 131 DAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAV-DLVKTVF 189
D++G+ E L F+ + S S L + P EAE + + +
Sbjct: 124 DSIGAKE------------LAKDFVVSGTASES-LYGACEAMYKPDMEAEELFETISQAL 170
Query: 190 ASATERDIYTGDKVEIVILNAGGIHREFMDLRKD 223
S+ +RD +G + I+ I + R D
Sbjct: 171 LSSVDRDCLSGWGGHVYIVTPTEIKERILKGRMD 204
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.318 0.133 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,566,209
Number of Sequences: 164201
Number of extensions: 989135
Number of successful extensions: 2560
Number of sequences better than 10.0: 107
Number of HSP's better than 10.0 without gapping: 74
Number of HSP's successfully gapped in prelim test: 33
Number of HSP's that attempted gapping in prelim test: 2440
Number of HSP's gapped (non-prelim): 107
length of query: 223
length of database: 59,974,054
effective HSP length: 106
effective length of query: 117
effective length of database: 42,568,748
effective search space: 4980543516
effective search space used: 4980543516
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 64 (29.3 bits)
Medicago: description of AC146330.2