BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146308.4 + phase: 0
(357 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YM53_MYCBO (P64956) Hypothetical protein Mb2253c 96 1e-19
YM28_MYCTU (P64955) Hypothetical protein Rv2228c/MT2287 96 1e-19
RNHL_BACSU (P54162) 14.7 kDa ribonuclease H-like protein 56 1e-07
EBSB_ENTFA (P36921) Cell wall enzyme ebsB 44 6e-04
RNH_HELPY (P56120) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 43 0.001
RNH_HELPJ (Q9ZLH3) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 42 0.002
RNH_RALSO (Q8XZ91) Ribonuclease H (EC 3.1.26.4) (RNase H) 42 0.002
RNH_SALTY (P23329) Ribonuclease HI (EC 3.1.26.4) (RNase HI) (Rib... 39 0.020
RNH_STRCO (Q9X7R6) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 39 0.026
RNH_PASMU (P57813) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 39 0.026
RNH_HAEDU (Q7VM15) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 39 0.026
RNH_NEIMB (Q9JYE5) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 37 0.059
RNH_NEIMA (Q9JTD9) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 37 0.059
RNH_CAUCR (Q9A341) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 37 0.059
RNH_YERPE (Q8ZH30) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 37 0.100
RNH_VIBVU (Q8DBD5) Ribonuclease HI (EC 3.1.26.4) (RNase HI) 37 0.100
RNH_WIGBR (Q8D3D3) Ribonuclease H (EC 3.1.26.4) (RNase H) 36 0.13
RNH_BUCAP (Q08885) Ribonuclease H (EC 3.1.26.4) (RNase H) 36 0.13
YPEP_BACSU (P54164) Hypothetical protein ypeP 36 0.17
RNH_ECOLI (P00647) Ribonuclease HI (EC 3.1.26.4) (RNase HI) (Rib... 36 0.17
>YM53_MYCBO (P64956) Hypothetical protein Mb2253c
Length = 364
Score = 95.9 bits (237), Expect = 1e-19
Identities = 55/136 (40%), Positives = 82/136 (59%), Gaps = 4/136 (2%)
Query: 204 IVEFDGASKGNPGKAGAGAILRSKD-GNLIYRVREGVGIATNNVAEYRAMILGMRHALKK 262
++E DG S+GNPG AG GA++ + D ++ ++ +G ATNNVAEYR +I G+ A+K
Sbjct: 4 VIEADGGSRGNPGPAGYGAVVWTADHSTVLAESKQAIGRATNNVAEYRGLIAGLDDAVKL 63
Query: 263 GFTSICIQGDSKLVCMQMKQIDGLWKVKNENLSTLYKVAKELKDKFVSFKISHVLRDLNS 322
G T + DSKLV ++Q+ G WKVK+ +L LY A+ L +F V R N+
Sbjct: 64 GATEAAVLMDSKLV---VEQMSGRWKVKHPDLLKLYVQAQALASQFRRINYEWVPRARNT 120
Query: 323 EADAQANLAVNLAGEA 338
AD AN A++ A ++
Sbjct: 121 YADRLANDAMDAAAQS 136
>YM28_MYCTU (P64955) Hypothetical protein Rv2228c/MT2287
Length = 364
Score = 95.9 bits (237), Expect = 1e-19
Identities = 55/136 (40%), Positives = 82/136 (59%), Gaps = 4/136 (2%)
Query: 204 IVEFDGASKGNPGKAGAGAILRSKD-GNLIYRVREGVGIATNNVAEYRAMILGMRHALKK 262
++E DG S+GNPG AG GA++ + D ++ ++ +G ATNNVAEYR +I G+ A+K
Sbjct: 4 VIEADGGSRGNPGPAGYGAVVWTADHSTVLAESKQAIGRATNNVAEYRGLIAGLDDAVKL 63
Query: 263 GFTSICIQGDSKLVCMQMKQIDGLWKVKNENLSTLYKVAKELKDKFVSFKISHVLRDLNS 322
G T + DSKLV ++Q+ G WKVK+ +L LY A+ L +F V R N+
Sbjct: 64 GATEAAVLMDSKLV---VEQMSGRWKVKHPDLLKLYVQAQALASQFRRINYEWVPRARNT 120
Query: 323 EADAQANLAVNLAGEA 338
AD AN A++ A ++
Sbjct: 121 YADRLANDAMDAAAQS 136
>RNHL_BACSU (P54162) 14.7 kDa ribonuclease H-like protein
Length = 132
Score = 56.2 bits (134), Expect = 1e-07
Identities = 39/127 (30%), Positives = 61/127 (47%), Gaps = 6/127 (4%)
Query: 208 DGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHALKKGFTSI 267
DGAS GNPG +G G ++ + + + +G+ TN AE+ A+I GM+ +G+ S+
Sbjct: 8 DGASAGNPGPSGIGIFIKHEGKAESFSIP--IGVHTNQEAEFLALIEGMKLCATRGYQSV 65
Query: 268 CIQGDSKLVCMQMKQIDGLWKVKNENLSTLYKVAKELKDKFVSFKISHVLRDLNSEADAQ 327
+ DS +V ++ L VKN + LK F F I + N +AD
Sbjct: 66 SFRTDSDIV----ERATELEMVKNITFQPFVEEIIRLKAAFPLFFIKWIPGKQNQKADLL 121
Query: 328 ANLAVNL 334
A A+ L
Sbjct: 122 AKEAIRL 128
>EBSB_ENTFA (P36921) Cell wall enzyme ebsB
Length = 135
Score = 43.9 bits (102), Expect = 6e-04
Identities = 31/127 (24%), Positives = 60/127 (46%), Gaps = 8/127 (6%)
Query: 208 DGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHAL--KKGFT 265
D A+KGNPG++G G + + ++ +GI +N+ AE++ +I ++ A+ +
Sbjct: 7 DAATKGNPGESGGGIVYLTDQSR--QQLHVPLGIVSNHEAEFKVLIEALKKAIANEDNQQ 64
Query: 266 SICIQGDSKLVCMQMKQIDGLWKVKNENLSTLYKVAKELKDKFVSFKISHVLRDLNSEAD 325
++ + DSK+V +++ KNE ++L+ F I + N AD
Sbjct: 65 TVLLHSDSKIVVQTIEKN----YAKNEKYQPYLAEYQQLEKNFPLLLIEWLPESQNKAAD 120
Query: 326 AQANLAV 332
A A+
Sbjct: 121 MLARQAL 127
>RNH_HELPY (P56120) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 143
Score = 42.7 bits (99), Expect = 0.001
Identities = 30/84 (35%), Positives = 40/84 (46%), Gaps = 4/84 (4%)
Query: 208 DGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHALKKGFTSI 267
DG+S GNPG G AILR KD + G TNN E RA+ ++ + K I
Sbjct: 9 DGSSLGNPGPGGYAAILRYKDKE--KTISGGEEFTTNNRMELRALNEALK--ILKRPCRI 64
Query: 268 CIQGDSKLVCMQMKQIDGLWKVKN 291
+ DS+ VC + W+ KN
Sbjct: 65 TLYSDSQYVCQAINVWLANWQKKN 88
>RNH_HELPJ (Q9ZLH3) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 143
Score = 42.4 bits (98), Expect = 0.002
Identities = 30/84 (35%), Positives = 40/84 (46%), Gaps = 4/84 (4%)
Query: 208 DGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHALKKGFTSI 267
DG+S GNPG G AILR KD + G TNN E RA+ ++ + K I
Sbjct: 9 DGSSLGNPGPGGYAAILRYKDKE--KTISGGENFTTNNRMELRALNEALK--ILKRPCHI 64
Query: 268 CIQGDSKLVCMQMKQIDGLWKVKN 291
+ DS+ VC + W+ KN
Sbjct: 65 TLYSDSQYVCQAINVWLVNWQKKN 88
>RNH_RALSO (Q8XZ91) Ribonuclease H (EC 3.1.26.4) (RNase H)
Length = 151
Score = 42.0 bits (97), Expect = 0.002
Identities = 43/144 (29%), Positives = 63/144 (42%), Gaps = 15/144 (10%)
Query: 208 DGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHALKKGFTSI 267
DGA KGNPG G G +L S G + G + TNN E A+I R ALK+ +
Sbjct: 9 DGACKGNPGLGGWGTVLVS--GGHEKELFGGEAVTTNNRMELMAVIEAFR-ALKRP-CRV 64
Query: 268 CIQGDSKLVCMQMKQIDGLWKVKNENLSTLYKVAKE-----LKDKFVSFKIS------HV 316
+ DS+ V + + WK + + V + L + V+ ++S H
Sbjct: 65 KVYTDSQYVQKGISEWLAGWKARGWKTADKKPVKNDDLWRTLDELVVTHEVSWHWVKGHA 124
Query: 317 LRDLNSEADAQANLAVNLAGEAFK 340
N ADA AN V +A +A +
Sbjct: 125 GHPGNERADALANKGVEIARQAIQ 148
>RNH_SALTY (P23329) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
(Ribonuclease H) (RNase H)
Length = 155
Score = 38.9 bits (89), Expect = 0.020
Identities = 29/81 (35%), Positives = 40/81 (48%), Gaps = 4/81 (4%)
Query: 208 DGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHALKKGFTSI 267
DG+ GNPG G GAILR + + EG + TNN E A I+ + ALK+ +
Sbjct: 10 DGSCLGNPGPGGYGAILRYRGHEKTF--SEGYTLTTNNRMELMAAIVAL-EALKE-HCEV 65
Query: 268 CIQGDSKLVCMQMKQIDGLWK 288
+ DS+ V + Q WK
Sbjct: 66 TLSTDSQYVRQGITQWIHNWK 86
>RNH_STRCO (Q9X7R6) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 231
Score = 38.5 bits (88), Expect = 0.026
Identities = 20/45 (44%), Positives = 26/45 (57%)
Query: 208 DGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAM 252
DGASKGNPG AG ++ N + +G ATNN+AE A+
Sbjct: 10 DGASKGNPGPAGWAWVVADASENPVRWEAGPLGKATNNIAELTAL 54
>RNH_PASMU (P57813) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 154
Score = 38.5 bits (88), Expect = 0.026
Identities = 29/89 (32%), Positives = 44/89 (48%), Gaps = 4/89 (4%)
Query: 208 DGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHALKKGFTSI 267
DG+ GNPG G G +LR K ++ G + TNN E RA+I + + LK+ S+
Sbjct: 10 DGSCLGNPGPGGIGVLLRYKQHE--KQISAGYFLTTNNRMELRAVIEAL-NTLKEP-CSV 65
Query: 268 CIQGDSKLVCMQMKQIDGLWKVKNENLST 296
+ DS+ + + + WK N ST
Sbjct: 66 TLHSDSQYMKNGITKWIFNWKKNNWKAST 94
>RNH_HAEDU (Q7VM15) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 153
Score = 38.5 bits (88), Expect = 0.026
Identities = 32/107 (29%), Positives = 51/107 (46%), Gaps = 6/107 (5%)
Query: 200 VKTCIVEFDGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHA 259
+K+ + DG+ GNPG G G +LR +V +G TNN E RA+I G+ +
Sbjct: 1 MKSVNIFTDGSCLGNPGPGGIGVVLRYNQHQ--KKVSQGYFQTTNNRMELRAVIEGL--S 56
Query: 260 LKKGFTSICIQGDSKLVCMQMKQIDGLWKVKNENLSTLYKVAKELKD 306
+ K ++ + DS+ M+ ++K K N T A + KD
Sbjct: 57 MLKEACNVTLYSDSQY--MKNGITKWIFKWKKSNWKTANGKAVKNKD 101
>RNH_NEIMB (Q9JYE5) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 145
Score = 37.4 bits (85), Expect = 0.059
Identities = 29/88 (32%), Positives = 42/88 (46%), Gaps = 4/88 (4%)
Query: 201 KTCIVEFDGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHAL 260
+T + DGA KGNPG G G ++R G+ + G TNN E A+I G++
Sbjct: 3 QTVYLYTDGACKGNPGAGGWGVLMRY--GSHEKELFGGEAQTTNNRMELTAVIEGLKSLK 60
Query: 261 KKGFTSICIQGDSKLVCMQMKQIDGLWK 288
++ IC DS+ V M+ WK
Sbjct: 61 RRCTVIICT--DSQYVKNGMENWIHGWK 86
>RNH_NEIMA (Q9JTD9) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 145
Score = 37.4 bits (85), Expect = 0.059
Identities = 29/88 (32%), Positives = 42/88 (46%), Gaps = 4/88 (4%)
Query: 201 KTCIVEFDGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHAL 260
+T + DGA KGNPG G G ++R G+ + G TNN E A+I G++
Sbjct: 3 QTVYLYTDGACKGNPGAGGWGVLMRY--GSHEKELFGGEAQTTNNRMELTAVIEGLKSLK 60
Query: 261 KKGFTSICIQGDSKLVCMQMKQIDGLWK 288
++ IC DS+ V M+ WK
Sbjct: 61 RRCTVIICT--DSQYVKNGMENWIHGWK 86
>RNH_CAUCR (Q9A341) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 149
Score = 37.4 bits (85), Expect = 0.059
Identities = 41/133 (30%), Positives = 54/133 (39%), Gaps = 15/133 (11%)
Query: 208 DGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHALKKGFTSI 267
DGA KGNPG G GAIL G+ + G TNN E A I + L +
Sbjct: 10 DGACKGNPGPGGWGAILFY--GDKKKEICGGEPGTTNNRMELMAAIQALE--LLNRPCVV 65
Query: 268 CIQGDSKLVCMQMKQIDGLWK-----------VKNENLSTLYKVAKELKDKFVSFKISHV 316
+ DS+ V +++ WK VKN++L A+ D + H
Sbjct: 66 ELHTDSQYVMKGIQEWIRGWKARGWKTADKSPVKNDDLWKRLDAARARHDVDWRWVKGHA 125
Query: 317 LRDLNSEADAQAN 329
LN ADA AN
Sbjct: 126 GHPLNERADALAN 138
>RNH_YERPE (Q8ZH30) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 154
Score = 36.6 bits (83), Expect = 0.100
Identities = 29/84 (34%), Positives = 38/84 (44%), Gaps = 10/84 (11%)
Query: 208 DGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHALKKGFTSI 267
DG+ GNPG G GAILR K + G + TNN E A I+ + + TS
Sbjct: 10 DGSCLGNPGPGGYGAILRYKQHEKTFSA--GYYLTTNNRMELMAAIVAL-----EALTSP 62
Query: 268 C---IQGDSKLVCMQMKQIDGLWK 288
C + DS+ V + Q WK
Sbjct: 63 CEVTLSTDSQYVRQGITQWIHNWK 86
>RNH_VIBVU (Q8DBD5) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
Length = 155
Score = 36.6 bits (83), Expect = 0.100
Identities = 27/81 (33%), Positives = 42/81 (51%), Gaps = 4/81 (4%)
Query: 208 DGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHALKKGFTSI 267
DG+ GNPG G G +LR K + + +G + TNN E A I+ ++ ALK+ ++
Sbjct: 10 DGSCLGNPGPGGYGVVLRYK--QVEKTLAQGYRLTTNNRMEMMATIVALQ-ALKEP-CNV 65
Query: 268 CIQGDSKLVCMQMKQIDGLWK 288
+ DS+ V + Q WK
Sbjct: 66 ILTTDSQYVRQGITQWIHNWK 86
>RNH_WIGBR (Q8D3D3) Ribonuclease H (EC 3.1.26.4) (RNase H)
Length = 157
Score = 36.2 bits (82), Expect = 0.13
Identities = 31/108 (28%), Positives = 48/108 (43%), Gaps = 7/108 (6%)
Query: 208 DGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHALKKGFTSI 267
DGA GNPG G G I+ K L + G + TNN E A I+ + +I
Sbjct: 10 DGACLGNPGPGGYGVIICGK--KLYKEISNGYYLTTNNRMEIMAAIIALESL--HDMYNI 65
Query: 268 CIQGDSKLVCMQMKQIDGLW---KVKNENLSTLYKVAKELKDKFVSFK 312
I DSK + + + +W K K N ++ + +K +++S K
Sbjct: 66 IINTDSKYLKNGITKWIKVWKNNKWKTNNNKSVKNIDLWIKLEYLSKK 113
>RNH_BUCAP (Q08885) Ribonuclease H (EC 3.1.26.4) (RNase H)
Length = 161
Score = 36.2 bits (82), Expect = 0.13
Identities = 31/97 (31%), Positives = 43/97 (43%), Gaps = 5/97 (5%)
Query: 208 DGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHALKKGFTSI 267
DG+ GNPG G G ILR K I + G + TNN E +I G+ + I
Sbjct: 10 DGSCLGNPGSGGYGTILRYKLHEKI--LTSGFFLTTNNRMELMGVICGLESLKESCIVEI 67
Query: 268 CIQGDSKLVCMQMKQIDGLWKVKNENLSTLYKVAKEL 304
I DS+ V + W+ K + +T K+ K L
Sbjct: 68 TI--DSQYVKQGITNWIATWE-KKKWKTTKKKLIKNL 101
>YPEP_BACSU (P54164) Hypothetical protein ypeP
Length = 120
Score = 35.8 bits (81), Expect = 0.17
Identities = 32/101 (31%), Positives = 48/101 (46%), Gaps = 12/101 (11%)
Query: 234 RVREGVGIATNNVAEYRAMILGMRHALKKGFT--SICIQGDSKLVCMQMKQIDGLWKV-- 289
R + + TNN AEY A+ +R + G + SI I+GDS +V + Q+DG W
Sbjct: 2 RKNKSFRLKTNNEAEYAALYEAIREVRELGASRNSITIKGDSLVV---LNQLDGSWPCYD 58
Query: 290 --KNENLSTLYKVAKELKDKFVSFKISHVLRDLNSEADAQA 328
NE L + + + LK ++ + R N EAD A
Sbjct: 59 PSHNEWLDKIEALLESLK---LTPTYETIQRKDNQEADGLA 96
>RNH_ECOLI (P00647) Ribonuclease HI (EC 3.1.26.4) (RNase HI)
(Ribonuclease H) (RNase H)
Length = 155
Score = 35.8 bits (81), Expect = 0.17
Identities = 32/86 (37%), Positives = 40/86 (46%), Gaps = 14/86 (16%)
Query: 208 DGASKGNPGKAGAGAILRSKDGNLIYRVRE-----GVGIATNNVAEYRAMILGMRHALKK 262
DG+ GNPG G GAILR YR RE G TNN E A I+ + ALK+
Sbjct: 10 DGSCLGNPGPGGYGAILR-------YRGREKTFSAGYTRTTNNRMELMAAIVAL-EALKE 61
Query: 263 GFTSICIQGDSKLVCMQMKQIDGLWK 288
+ + DS+ V + Q WK
Sbjct: 62 -HCEVILSTDSQYVRQGITQWIHNWK 86
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.317 0.131 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,630,742
Number of Sequences: 164201
Number of extensions: 1556303
Number of successful extensions: 4390
Number of sequences better than 10.0: 61
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 50
Number of HSP's that attempted gapping in prelim test: 4366
Number of HSP's gapped (non-prelim): 64
length of query: 357
length of database: 59,974,054
effective HSP length: 111
effective length of query: 246
effective length of database: 41,747,743
effective search space: 10269944778
effective search space used: 10269944778
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 66 (30.0 bits)
Medicago: description of AC146308.4