Medicago
BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC145221.10 - phase: 0 
         (68 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

YHJ1_YEAST (P38766) Hypothetical helicase in SLT2-PUT2 intergeni...    31  0.52
EX5A_HAEIN (P45158) Exodeoxyribonuclease V alpha chain (EC 3.1.1...    29  2.6
EX5A_ECOLI (P04993) Exodeoxyribonuclease V alpha chain (EC 3.1.1...    28  4.4
YEJH_ECOLI (P33919) Hypothetical protein yejH                          27  7.5
FABH_CHLPN (Q9Z8P0) 3-oxoacyl-[acyl-carrier-protein] synthase II...    27  7.5
YC22_METJA (Q58619) Hypothetical protein MJ1222                        27  9.8
RPOC_VIBPA (Q87KQ5) DNA-directed RNA polymerase beta' chain (EC ...    27  9.8
FCP1_HUMAN (Q9Y5B0) RNA polymerase II subunit A C-terminal domai...    27  9.8

>YHJ1_YEAST (P38766) Hypothetical helicase in SLT2-PUT2 intergenic
           region
          Length = 723

 Score = 31.2 bits (69), Expect = 0.52
 Identities = 18/64 (28%), Positives = 37/64 (57%), Gaps = 1/64 (1%)

Query: 1   MTINKSQGQSLKHDGVYLPTPVFSHGQLHVVVSKVTSREWLKILIIDEDGEDTDETSNIV 60
           ++I+K+QGQ+++   V L   +F  GQ++V +S+  + + L++L  D     T+E     
Sbjct: 657 LSIHKAQGQTIQRLKVDLRR-IFEAGQVYVALSRAVTMDTLQVLNFDPGKIRTNERVKDF 715

Query: 61  YEEV 64
           Y+ +
Sbjct: 716 YKRL 719


>EX5A_HAEIN (P45158) Exodeoxyribonuclease V alpha chain (EC
           3.1.11.5)
          Length = 640

 Score = 28.9 bits (63), Expect = 2.6
 Identities = 13/22 (59%), Positives = 15/22 (68%)

Query: 1   MTINKSQGQSLKHDGVYLPTPV 22
           MTI+KSQG   KH  + LPT V
Sbjct: 568 MTIHKSQGSEFKHTVMVLPTEV 589


>EX5A_ECOLI (P04993) Exodeoxyribonuclease V alpha chain (EC
           3.1.11.5) (Exodeoxyribonuclease V 67 kDa polypeptide)
          Length = 608

 Score = 28.1 bits (61), Expect = 4.4
 Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 3/41 (7%)

Query: 1   MTINKSQGQSLKHDGVYLP---TPVFSHGQLHVVVSKVTSR 38
           MT++KSQG    H  + LP   TPV +   ++  V++   R
Sbjct: 536 MTVHKSQGSEFDHAALILPSQRTPVVTRELVYTAVTRARRR 576


>YEJH_ECOLI (P33919) Hypothetical protein yejH
          Length = 586

 Score = 27.3 bits (59), Expect = 7.5
 Identities = 11/17 (64%), Positives = 11/17 (64%)

Query: 39  EWLKILIIDEDGEDTDE 55
           EWLKI   DEDG D  E
Sbjct: 490 EWLKITYYDEDGADVSE 506


>FABH_CHLPN (Q9Z8P0) 3-oxoacyl-[acyl-carrier-protein] synthase III
          (EC 2.3.1.41) (Beta-ketoacyl-ACP synthase III) (KAS
          III)
          Length = 335

 Score = 27.3 bits (59), Expect = 7.5
 Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 2/40 (5%)

Query: 2  TINKSQGQSLKHDGVYLPTPVFSHGQLHVVVSKVTSREWL 41
          ++NK++  ++   G YLP  V S+  L  +V   TS EW+
Sbjct: 4  SVNKNKKAAIWATGSYLPEKVLSNADLEKMVD--TSDEWI 41


>YC22_METJA (Q58619) Hypothetical protein MJ1222
          Length = 243

 Score = 26.9 bits (58), Expect = 9.8
 Identities = 16/63 (25%), Positives = 30/63 (47%), Gaps = 1/63 (1%)

Query: 1  MTINKSQGQSLKHDGVYLPTPVFSHGQLHVVVSKVTSREWLKILIIDEDGEDTDETSNIV 60
          M    SQ   +  D +++  P F+  ++     K   +E  K +++ +DG   D+TS I 
Sbjct: 4  MKKQNSQINEINKDEIFVVVPAFNEEKMIGETLKNLKKEGYKNIVVVDDG-SMDKTSEIA 62

Query: 61 YEE 63
           +E
Sbjct: 63 KKE 65


>RPOC_VIBPA (Q87KQ5) DNA-directed RNA polymerase beta' chain (EC
           2.7.7.6) (RNAP beta' subunit) (Transcriptase beta'
           chain) (RNA polymerase beta' subunit)
          Length = 1400

 Score = 26.9 bits (58), Expect = 9.8
 Identities = 14/52 (26%), Positives = 28/52 (52%), Gaps = 2/52 (3%)

Query: 10  SLKHDGVYLPTPVFSHGQLHVVVSKVTSREWLKIL--IIDEDGEDTDETSNI 59
           ++K +G+YL  P  +        +++ +R  ++I   ++DEDG  T ET  +
Sbjct: 519 NVKGEGMYLSGPAEAEKAYRTKQAELHARVKVRITETVVDEDGNSTTETKMV 570


>FCP1_HUMAN (Q9Y5B0) RNA polymerase II subunit A C-terminal domain
           phosphatase (EC 3.1.3.16) (TFIIF-associating CTD
           phosphatase)
          Length = 961

 Score = 26.9 bits (58), Expect = 9.8
 Identities = 15/38 (39%), Positives = 24/38 (62%), Gaps = 1/38 (2%)

Query: 32  VSKVTSREWLKILIIDEDGEDTDETSNIVY-EEVFQNV 68
           +S VT+ E L   + +E+ EDTDE  +++Y EE+   V
Sbjct: 563 LSGVTAGESLDQSMEEEEEEDTDEDDHLIYLEEILVRV 600


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.314    0.133    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,111,629
Number of Sequences: 164201
Number of extensions: 267821
Number of successful extensions: 474
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 472
Number of HSP's gapped (non-prelim): 8
length of query: 68
length of database: 59,974,054
effective HSP length: 44
effective length of query: 24
effective length of database: 52,749,210
effective search space: 1265981040
effective search space used: 1265981040
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 58 (26.9 bits)


Medicago: description of AC145221.10