Medicago
BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC144723.3 + phase: 0 
         (127 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

LMA_DROME (Q00174) Laminin alpha chain precursor                       28  2.7
TIE1_HUMAN (P35590) Tyrosine-protein kinase receptor Tie-1 precu...    28  4.7
LMB3_MOUSE (Q61087) Laminin beta-3 chain precursor (Laminin 5 be...    28  4.7
PCK5_BRACL (Q9NJ15) Proprotein convertase subtilisin/kexin type ...    27  8.0
G3P1_CANGA (Q6FPW3) Glyceraldehyde-3-phosphate dehydrogenase 1 (...    27  8.0

>LMA_DROME (Q00174) Laminin alpha chain precursor
          Length = 3712

 Score = 28.5 bits (62), Expect = 2.7
 Identities = 25/83 (30%), Positives = 32/83 (38%), Gaps = 18/83 (21%)

Query: 27   CKGPKYPPKECCG-SFKEFACPYADVINDLTNDCASTMFSYINLYGRY-----PPGLF-- 78
            CK  K P    C  +  E  CP  +VI DL   CA   + +  + G       P G+   
Sbjct: 1418 CKPCKCPNSAMCEPTTGECMCP-PNVIGDLCEKCAPNTYGFHQVIGCEECACNPMGIANG 1476

Query: 79   ---------ASECREGKEGLACD 92
                       ECR+  EG ACD
Sbjct: 1477 NSQCDLFNGTCECRQNIEGRACD 1499


>TIE1_HUMAN (P35590) Tyrosine-protein kinase receptor Tie-1
           precursor (EC 2.7.1.112)
          Length = 1138

 Score = 27.7 bits (60), Expect = 4.7
 Identities = 22/87 (25%), Positives = 33/87 (37%), Gaps = 25/87 (28%)

Query: 20  YTIITSKCKGPKYPP---KECCGSFKEFACPYADVINDLTNDCASTMFSYINLYGRYPPG 76
           + +I   C   ++ P   KEC G      C +  V +D   +C              PPG
Sbjct: 208 FRLIVRGCGAGRWGPGCTKECPG------CLHGGVCHDHDGECVC------------PPG 249

Query: 77  LFASEC----REGKEGLACDALPPSVS 99
              + C    REG+ G +C    P +S
Sbjct: 250 FTGTRCEQACREGRFGQSCQEQCPGIS 276


>LMB3_MOUSE (Q61087) Laminin beta-3 chain precursor (Laminin 5 beta
           3) (Kalinin B1 chain)
          Length = 1168

 Score = 27.7 bits (60), Expect = 4.7
 Identities = 17/48 (35%), Positives = 20/48 (41%), Gaps = 1/48 (2%)

Query: 46  CPYADVINDLTNDCASTMFSYINLYGRYPPGLFASECR-EGKEGLACD 92
           CP  +    LT   A+        YG  P G  A +C   G EG ACD
Sbjct: 497 CPCREGFGGLTCSSAAIRQCPDQTYGHVPTGCRACDCDFRGTEGPACD 544


>PCK5_BRACL (Q9NJ15) Proprotein convertase subtilisin/kexin type 5
            precursor (EC 3.4.21.-) (Proprotein convertase PC6-like)
            (aPC6)
          Length = 1696

 Score = 26.9 bits (58), Expect = 8.0
 Identities = 24/79 (30%), Positives = 35/79 (43%), Gaps = 8/79 (10%)

Query: 8    PAGCSVNFEFLNYTIITSKCKGPKYPPKECCGSFKEFACPYADVINDLTNDCASTMFSYI 67
            PAG   N E  ++  + S C+  +Y   E  G  ++  CPY     D   DCA    +YI
Sbjct: 1152 PAGFIGNAE--SHECVESSCEQDQYYSSET-GRCED--CPYNCRACDNDGDCAECAPTYI 1206

Query: 68   NLYGRYPPGLFASECREGK 86
             + GR  P      C +G+
Sbjct: 1207 VVDGRCRP---EETCEDGE 1222


>G3P1_CANGA (Q6FPW3) Glyceraldehyde-3-phosphate dehydrogenase 1
          (EC 1.2.1.12) (GAPDH 1)
          Length = 332

 Score = 26.9 bits (58), Expect = 8.0
 Identities = 13/24 (54%), Positives = 17/24 (70%), Gaps = 2/24 (8%)

Query: 52 IND--LTNDCASTMFSYINLYGRY 73
          IND  +TND A+ MF Y + +GRY
Sbjct: 31 INDPFITNDYAAYMFKYDSTHGRY 54


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.325    0.141    0.454 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,961,033
Number of Sequences: 164201
Number of extensions: 621189
Number of successful extensions: 1576
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1575
Number of HSP's gapped (non-prelim): 6
length of query: 127
length of database: 59,974,054
effective HSP length: 103
effective length of query: 24
effective length of database: 43,061,351
effective search space: 1033472424
effective search space used: 1033472424
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 58 (26.9 bits)


Medicago: description of AC144723.3