BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC141109.13 - phase: 0
(208 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MAN2_DROME (Q24451) Alpha-mannosidase II (EC 3.2.1.114) (Mannosy... 95 1e-19
M2A2_HUMAN (P49641) Alpha-mannosidase IIx (EC 3.2.1.114) (Mannos... 89 7e-18
M2A1_MOUSE (P27046) Alpha-mannosidase II (EC 3.2.1.114) (Mannosy... 88 1e-17
M2A1_RAT (P28494) Alpha-mannosidase II (EC 3.2.1.114) (Mannosyl-... 88 2e-17
M2A1_HUMAN (Q16706) Alpha-mannosidase II (EC 3.2.1.114) (Mannosy... 87 3e-17
M2B2_PIG (Q28949) Epididymis-specific alpha-mannosidase precurso... 43 6e-04
M2B2_HUMAN (Q9Y2E5) Epididymis-specific alpha-mannosidase precur... 40 0.005
M2B2_MOUSE (O54782) Epididymis-specific alpha-mannosidase precur... 38 0.015
M2B1_MOUSE (O09159) Lysosomal alpha-mannosidase precursor (EC 3.... 37 0.025
M2B1_BOVIN (Q29451) Lysosomal alpha-mannosidase precursor (EC 3.... 37 0.043
M2B1_CAVPO (Q8VHC8) Lysosomal alpha-mannosidase precursor (EC 3.... 35 0.095
M2B1_HUMAN (O00754) Lysosomal alpha-mannosidase precursor (EC 3.... 35 0.12
MANA_DICDI (P34098) Lysosomal alpha-mannosidase precursor (EC 3.... 35 0.16
M2B1_FELCA (O46432) Lysosomal alpha-mannosidase precursor (EC 3.... 34 0.28
AROA_BRUSU (Q8G3C4) 3-phosphoshikimate 1-carboxyvinyltransferase... 34 0.28
AROA_BRUME (Q8YEG1) 3-phosphoshikimate 1-carboxyvinyltransferase... 34 0.28
AROA_BRUAB (Q9AGV2) 3-phosphoshikimate 1-carboxyvinyltransferase... 34 0.28
NU5M_CAEEL (P24896) NADH-ubiquinone oxidoreductase chain 5 (EC 1... 32 0.81
AROA_PSES2 (P56952) 3-phosphoshikimate 1-carboxyvinyltransferase... 32 1.1
ANX3_HUMAN (P12429) Annexin A3 (Annexin III) (Lipocortin III) (P... 31 1.8
>MAN2_DROME (Q24451) Alpha-mannosidase II (EC 3.2.1.114)
(Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase)
(MAN II) (Golgi alpha-mannosidase II) (AMAN II)
Length = 1108
Score = 94.7 bits (234), Expect = 1e-19
Identities = 46/99 (46%), Positives = 62/99 (62%), Gaps = 4/99 (4%)
Query: 99 SVDLTTKGLYDKIEFLDVDGGAWKQGWSVSYRGDEWD-NEKLKVFVVPHSHNDPGWKLTV 157
+VD+ LYD++ F D+DGG WKQGW++ Y +++ + KLKVFVVPHSHNDPGW T
Sbjct: 103 NVDVQMLELYDRMSFKDIDGGVWKQGWNIKYDPLKYNAHHKLKVFVVPHSHNDPGWIQTF 162
Query: 158 EEYYDRQSRHILDTIVETL---SKVKFCLFPFLGFGDFY 193
EEYY ++HIL + L ++KF F FY
Sbjct: 163 EEYYQHDTKHILSNALRHLHDNPEMKFIWAEISYFARFY 201
>M2A2_HUMAN (P49641) Alpha-mannosidase IIx (EC 3.2.1.114)
(Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase)
(MAN IIx) (Mannosidase alpha class 2A member 2)
Length = 1139
Score = 89.0 bits (219), Expect = 7e-18
Identities = 36/70 (51%), Positives = 53/70 (75%)
Query: 109 DKIEFLDVDGGAWKQGWSVSYRGDEWDNEKLKVFVVPHSHNDPGWKLTVEEYYDRQSRHI 168
+++ F +VDGG W+QG+ +SY +WD E L+VFVVPHSHNDPGW T ++YY Q++HI
Sbjct: 136 EELPFDNVDGGVWRQGFDISYDPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHI 195
Query: 169 LDTIVETLSK 178
L+++V L +
Sbjct: 196 LNSMVSKLQE 205
>M2A1_MOUSE (P27046) Alpha-mannosidase II (EC 3.2.1.114)
(Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase)
(MAN II) (Golgi alpha-mannosidase II) (Mannosidase alpha
class 2A member 1) (AMAN II)
Length = 1150
Score = 88.2 bits (217), Expect = 1e-17
Identities = 37/78 (47%), Positives = 53/78 (67%)
Query: 101 DLTTKGLYDKIEFLDVDGGAWKQGWSVSYRGDEWDNEKLKVFVVPHSHNDPGWKLTVEEY 160
D+ +YD I F + DGG WKQG+ + Y DEWD+E L+VFVVPHSHNDPGW T +Y
Sbjct: 127 DVQMLDVYDLIPFDNPDGGVWKQGFDIKYEADEWDHEPLQVFVVPHSHNDPGWLKTFNDY 186
Query: 161 YDRQSRHILDTIVETLSK 178
+ ++++I + +V L +
Sbjct: 187 FRDKTQYIFNNMVLKLKE 204
>M2A1_RAT (P28494) Alpha-mannosidase II (EC 3.2.1.114)
(Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase)
(MAN II) (Golgi alpha-mannosidase II) (Mannosidase alpha
class 2A member 1) (Fragment)
Length = 489
Score = 87.8 bits (216), Expect = 2e-17
Identities = 37/78 (47%), Positives = 52/78 (66%)
Query: 101 DLTTKGLYDKIEFLDVDGGAWKQGWSVSYRGDEWDNEKLKVFVVPHSHNDPGWKLTVEEY 160
D+ +YD I F + DGG WKQG+ + Y DEWD E L+VFVVPHSHNDPGW T +Y
Sbjct: 94 DVQMLDVYDLIPFDNPDGGVWKQGFDIKYEADEWDREPLQVFVVPHSHNDPGWLKTFNDY 153
Query: 161 YDRQSRHILDTIVETLSK 178
+ ++++I + +V L +
Sbjct: 154 FRDKTQYIFNNMVLKLKE 171
>M2A1_HUMAN (Q16706) Alpha-mannosidase II (EC 3.2.1.114)
(Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase)
(MAN II) (Golgi alpha-mannosidase II) (Mannosidase alpha
class 2A member 1)
Length = 1144
Score = 87.0 bits (214), Expect = 3e-17
Identities = 44/119 (36%), Positives = 68/119 (56%), Gaps = 5/119 (4%)
Query: 65 PKPITTHFRTRTSRFRKPKKLSLNGSST--IFG---GFASVDLTTKGLYDKIEFLDVDGG 119
PK ++F P +LSL+ + +F G + D+ +Y I F + DGG
Sbjct: 87 PKSSQSNFSQGAGSHLLPSQLSLSVDTADCLFASQSGSHNSDVQMLDVYSLISFDNPDGG 146
Query: 120 AWKQGWSVSYRGDEWDNEKLKVFVVPHSHNDPGWKLTVEEYYDRQSRHILDTIVETLSK 178
WKQG+ ++Y +EWD E L+VFVVPHSHNDPGW T +Y+ ++++I + +V L +
Sbjct: 147 VWKQGFDITYESNEWDTEPLQVFVVPHSHNDPGWLKTFNDYFRDKTQYIFNNMVLKLKE 205
>M2B2_PIG (Q28949) Epididymis-specific alpha-mannosidase precursor
(EC 3.2.1.24) (Mannosidase alpha class 2B member 2)
(AMAN)
Length = 995
Score = 42.7 bits (99), Expect = 6e-04
Identities = 20/43 (46%), Positives = 28/43 (64%)
Query: 138 KLKVFVVPHSHNDPGWKLTVEEYYDRQSRHILDTIVETLSKVK 180
K+KVFVVPHSH D GW TV+E + +++VE L++ K
Sbjct: 26 KVKVFVVPHSHMDVGWLHTVQESMQVYVPDVYNSVVEALTRGK 68
>M2B2_HUMAN (Q9Y2E5) Epididymis-specific alpha-mannosidase precursor
(EC 3.2.1.24) (Mannosidase alpha class 2B member 2)
Length = 1009
Score = 39.7 bits (91), Expect = 0.005
Identities = 16/40 (40%), Positives = 26/40 (65%)
Query: 139 LKVFVVPHSHNDPGWKLTVEEYYDRQSRHILDTIVETLSK 178
++ FVVPHSH D GW TV+E + ++ ++VE L++
Sbjct: 27 IRAFVVPHSHMDVGWVYTVQESMRAYAANVYTSVVEELAR 66
>M2B2_MOUSE (O54782) Epididymis-specific alpha-mannosidase precursor
(EC 3.2.1.24) (Mannosidase alpha class 2B member 2)
Length = 1018
Score = 38.1 bits (87), Expect = 0.015
Identities = 16/38 (42%), Positives = 23/38 (60%)
Query: 139 LKVFVVPHSHNDPGWKLTVEEYYDRQSRHILDTIVETL 176
++ FVVPHSH D GW TV+E + ++ T+V L
Sbjct: 27 IRAFVVPHSHMDVGWVFTVQESMRAYAANVYTTVVAEL 64
>M2B1_MOUSE (O09159) Lysosomal alpha-mannosidase precursor (EC
3.2.1.24) (Mannosidase, alpha B) (Lysosomal acid
alpha-mannosidase) (Laman) (Mannosidase alpha class 2B
member 1)
Length = 1013
Score = 37.4 bits (85), Expect = 0.025
Identities = 17/46 (36%), Positives = 28/46 (59%), Gaps = 8/46 (17%)
Query: 139 LKVFVVPHSHNDPGWKLTVEEYY--------DRQSRHILDTIVETL 176
L V ++PH+H+D GW TV++YY ++ILD++V +L
Sbjct: 63 LNVHLLPHTHDDVGWLKTVDQYYYGILSDVQHASVQYILDSVVSSL 108
>M2B1_BOVIN (Q29451) Lysosomal alpha-mannosidase precursor (EC
3.2.1.24) (Mannosidase, alpha B) (Lysosomal acid
alpha-mannosidase) (Laman) (Mannosidase alpha class 2B
member 1)
Length = 999
Score = 36.6 bits (83), Expect = 0.043
Identities = 18/61 (29%), Positives = 34/61 (55%), Gaps = 9/61 (14%)
Query: 125 WSVSYRG-DEWDNEKLKVFVVPHSHNDPGWKLTVEEYY--------DRQSRHILDTIVET 175
W+ Y+ + + L V +VPH+H+D GW TV++Y+ ++ILD+++ +
Sbjct: 49 WAAGYKTCPKVKPDMLNVHLVPHTHDDVGWLKTVDQYFYGIYNNIQPAGVQYILDSVISS 108
Query: 176 L 176
L
Sbjct: 109 L 109
>M2B1_CAVPO (Q8VHC8) Lysosomal alpha-mannosidase precursor (EC
3.2.1.24) (Mannosidase, alpha B) (Lysosomal acid
alpha-mannosidase) (Laman) (Mannosidase alpha class 2B
member 1)
Length = 1007
Score = 35.4 bits (80), Expect = 0.095
Identities = 18/46 (39%), Positives = 28/46 (60%), Gaps = 8/46 (17%)
Query: 139 LKVFVVPHSHNDPGWKLTVEEYY-----DRQS---RHILDTIVETL 176
L V +V H+H+D GW TV++YY D Q ++ILD+++ L
Sbjct: 61 LNVHLVAHTHDDVGWLKTVDQYYWGIHNDLQQAGVQYILDSVISAL 106
>M2B1_HUMAN (O00754) Lysosomal alpha-mannosidase precursor (EC
3.2.1.24) (Mannosidase, alpha B) (Lysosomal acid
alpha-mannosidase) (Laman) (Mannosidase alpha class 2B
member 1)
Length = 1010
Score = 35.0 bits (79), Expect = 0.12
Identities = 17/46 (36%), Positives = 29/46 (62%), Gaps = 8/46 (17%)
Query: 139 LKVFVVPHSHNDPGWKLTVEEYY-----DRQS---RHILDTIVETL 176
L V ++PH+H+D GW TV++Y+ D Q ++ILD+++ L
Sbjct: 62 LNVHLLPHTHDDVGWLKTVDQYFYGIKNDIQHAGVQYILDSVISAL 107
>MANA_DICDI (P34098) Lysosomal alpha-mannosidase precursor (EC
3.2.1.24) (Alpha-D-mannoside mannohydrolase) (Laman)
Length = 1005
Score = 34.7 bits (78), Expect = 0.16
Identities = 13/23 (56%), Positives = 17/23 (73%)
Query: 139 LKVFVVPHSHNDPGWKLTVEEYY 161
L V +V H+H+D GW TV+EYY
Sbjct: 42 LNVHIVAHTHDDVGWLKTVDEYY 64
>M2B1_FELCA (O46432) Lysosomal alpha-mannosidase precursor (EC
3.2.1.24) (Mannosidase, alpha B) (Lysosomal acid
alpha-mannosidase) (Laman) (Mannosidase alpha class 2B
member 1)
Length = 1007
Score = 33.9 bits (76), Expect = 0.28
Identities = 17/48 (35%), Positives = 30/48 (62%), Gaps = 8/48 (16%)
Query: 137 EKLKVFVVPHSHNDPGWKLTVEEYY-----DRQS---RHILDTIVETL 176
+ L V +V H+H+D GW TV++Y+ D Q ++ILD+++ +L
Sbjct: 62 DMLNVHLVAHTHDDVGWLKTVDQYFYGIHNDVQHAGVQYILDSVISSL 109
>AROA_BRUSU (Q8G3C4) 3-phosphoshikimate 1-carboxyvinyltransferase
(EC 2.5.1.19) (5-enolpyruvylshikimate-3-phosphate
synthase) (EPSP synthase) (EPSPS)
Length = 450
Score = 33.9 bits (76), Expect = 0.28
Identities = 21/71 (29%), Positives = 30/71 (41%), Gaps = 1/71 (1%)
Query: 65 PKPITT-HFRTRTSRFRKPKKLSLNGSSTIFGGFASVDLTTKGLYDKIEFLDVDGGAWKQ 123
PKP T H + T R P S++ S +FGG AS GL + + ++
Sbjct: 7 PKPATARHSQALTGEIRIPGDKSISHRSFMFGGLASGKTRITGLLEGEDVINTGRAMQAM 66
Query: 124 GWSVSYRGDEW 134
G + GD W
Sbjct: 67 GARIRKEGDVW 77
>AROA_BRUME (Q8YEG1) 3-phosphoshikimate 1-carboxyvinyltransferase
(EC 2.5.1.19) (5-enolpyruvylshikimate-3-phosphate
synthase) (EPSP synthase) (EPSPS)
Length = 450
Score = 33.9 bits (76), Expect = 0.28
Identities = 21/71 (29%), Positives = 30/71 (41%), Gaps = 1/71 (1%)
Query: 65 PKPITT-HFRTRTSRFRKPKKLSLNGSSTIFGGFASVDLTTKGLYDKIEFLDVDGGAWKQ 123
PKP T H + T R P S++ S +FGG AS GL + + ++
Sbjct: 7 PKPATARHSQALTGEIRIPGDKSISHRSFMFGGLASGKTRITGLLEGEDVINTGRAMQAM 66
Query: 124 GWSVSYRGDEW 134
G + GD W
Sbjct: 67 GARIRKEGDVW 77
>AROA_BRUAB (Q9AGV2) 3-phosphoshikimate 1-carboxyvinyltransferase
(EC 2.5.1.19) (5-enolpyruvylshikimate-3-phosphate
synthase) (EPSP synthase) (EPSPS)
Length = 450
Score = 33.9 bits (76), Expect = 0.28
Identities = 21/71 (29%), Positives = 30/71 (41%), Gaps = 1/71 (1%)
Query: 65 PKPITT-HFRTRTSRFRKPKKLSLNGSSTIFGGFASVDLTTKGLYDKIEFLDVDGGAWKQ 123
PKP T H + T R P S++ S +FGG AS GL + + ++
Sbjct: 7 PKPATARHSQALTGEIRIPGDKSISTRSFMFGGLASGKTRITGLLEGEDVINTGRAMQAM 66
Query: 124 GWSVSYRGDEW 134
G + GD W
Sbjct: 67 GARIRKEGDVW 77
>NU5M_CAEEL (P24896) NADH-ubiquinone oxidoreductase chain 5 (EC
1.6.5.3)
Length = 527
Score = 32.3 bits (72), Expect = 0.81
Identities = 26/69 (37%), Positives = 33/69 (47%), Gaps = 8/69 (11%)
Query: 36 KDFIFSNFFIIG--LIISLLFFLIVLLRFGVPKPITTHFRTRTSRFRKPKKLSLNGSSTI 93
KDFI FF + SL+FF+ V L FG FR S F K+ + SST+
Sbjct: 345 KDFILELFFSNNYMMFFSLMFFVSVFLTFGY------SFRLWKSFFLSFNKVMNHYSSTV 398
Query: 94 FGGFASVDL 102
F F S+ L
Sbjct: 399 FMNFLSLVL 407
>AROA_PSES2 (P56952) 3-phosphoshikimate 1-carboxyvinyltransferase
(EC 2.5.1.19) (5-enolpyruvylshikimate-3-phosphate
synthase) (EPSP synthase) (EPSPS)
Length = 449
Score = 32.0 bits (71), Expect = 1.1
Identities = 20/71 (28%), Positives = 29/71 (40%), Gaps = 1/71 (1%)
Query: 65 PKPITTH-FRTRTSRFRKPKKLSLNGSSTIFGGFASVDLTTKGLYDKIEFLDVDGGAWKQ 123
PKP T T R P S++ S +FGG AS + GL + + ++
Sbjct: 7 PKPATARRSEALTGEIRIPGDKSISHRSFMFGGLASGETRITGLLEGEDVINTGRAMQAM 66
Query: 124 GWSVSYRGDEW 134
G + GD W
Sbjct: 67 GAKIRKEGDVW 77
>ANX3_HUMAN (P12429) Annexin A3 (Annexin III) (Lipocortin III)
(Placental anticoagulant protein III) (PAP-III)
(35-alpha calcimedin) (Inositol 1,2-cyclic phosphate
2-phosphohydrolase)
Length = 323
Score = 31.2 bits (69), Expect = 1.8
Identities = 37/117 (31%), Positives = 49/117 (41%), Gaps = 15/117 (12%)
Query: 74 TRTSRFRKP----------KKLSLNGSSTIFGGFASVDLT-TKGLYDKIEFLDVDGGAWK 122
TRTSR K K L + SS G F LT G D E L VD K
Sbjct: 119 TRTSRQMKDISQAYYTVYKKSLGDDISSETSGDFRKALLTLADGRRD--ESLKVDEHLAK 176
Query: 123 QGWSVSYRGDE--WDNEKLKVFVVPHSHNDPGWKLTVEEYYDRQSRHILDTIVETLS 177
Q + Y+ E W ++ K + + P KLT +EY + + I+D+I LS
Sbjct: 177 QDAQILYKAGENRWGTDEDKFTEILCLRSFPQLKLTFDEYRNISQKDIVDSIKGELS 233
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.326 0.143 0.458
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,422,066
Number of Sequences: 164201
Number of extensions: 1057219
Number of successful extensions: 3923
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 3900
Number of HSP's gapped (non-prelim): 26
length of query: 208
length of database: 59,974,054
effective HSP length: 105
effective length of query: 103
effective length of database: 42,732,949
effective search space: 4401493747
effective search space used: 4401493747
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 63 (28.9 bits)
Medicago: description of AC141109.13