BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140104.6 - phase: 0
(216 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Y280_ARATH (O82368) Hypothetical protein At2g29880 37 0.027
WR57_ARATH (Q9C983) Probable WRKY transcription factor 57 (WRKY ... 34 0.23
SC17_COPCI (P78603) Vesicular-fusion protein SEC17 34 0.30
S521_HUMAN (Q96MU7) Putative splicing factor YT521 32 1.5
S521_RAT (Q9QY02) Putative splicing factor YT521 (RA301-binding ... 31 1.9
CHEA_RHOSH (Q53135) Chemotaxis protein cheA (EC 2.7.3.-) 31 1.9
RNE_ECOLI (P21513) Ribonuclease E (EC 3.1.4.-) (RNase E) 30 3.3
DA82_YEAST (P21705) DAL82 protein 30 3.3
SYNM_YEAST (P25345) Asparaginyl-tRNA synthetase, mitochondrial p... 30 4.3
VGLB_HSV2S (P24994) Glycoprotein B precursor 30 5.6
SPS_MAIZE (P31927) Sucrose-phosphate synthase (EC 2.4.1.14) (UDP... 30 5.6
ADEC_METKA (Q8TX47) Adenine deaminase (EC 3.5.4.2) (Adenase) (Ad... 30 5.6
SUS2_ARATH (Q00917) Sucrose synthase (EC 2.4.1.13) (Sucrose-UDP ... 29 7.3
FPG_MYCLE (O69470) Formamidopyrimidine-DNA glycosylase (EC 3.2.2... 29 7.3
ENV_BIV27 (P19557) Env polyprotein precursor (Coat polyprotein) ... 29 7.3
ENV_BIV06 (P19556) Env polyprotein precursor (Coat polyprotein) ... 29 7.3
CHS1_YEAST (P08004) Chitin synthase 1 (EC 2.4.1.16) (Chitin-UDP ... 29 7.3
>Y280_ARATH (O82368) Hypothetical protein At2g29880
Length = 308
Score = 37.4 bits (85), Expect = 0.027
Identities = 32/148 (21%), Positives = 63/148 (41%), Gaps = 8/148 (5%)
Query: 58 NKRANKNYTQKQLKNRMDTLRGEWTIWKQLLGKETGLGWNHHIGNIDA-DSAWWDAKIRN 116
NK+ N T +RM +++ E++++ L +G GW+ A D W + +
Sbjct: 63 NKKFKCNKTYTNYLSRMKSMKKEYSVYAALFWFSSGFGWDPITKQFTAPDDVWAAYLMGH 122
Query: 117 VKYAKFRFQGLEFCDELEFIFGEIVATSQCACAPAMGVPLESTGKNTTADVAREIIESDD 176
+ R E ++L+ IF +A A G+ +S N + +++ D
Sbjct: 123 PNHHHMRTSTFEDFEDLQLIFESAIAKGN----NAFGLGGDS---NAETFEEEDDLQAGD 175
Query: 177 ELNIDELSPMENTQSKNKRKVSPRMRKQ 204
+N E++ E ++ K K+ R R +
Sbjct: 176 NVNHMEINDDEVNETLPKEKLPTRKRSK 203
>WR57_ARATH (Q9C983) Probable WRKY transcription factor 57 (WRKY
DNA-binding protein 57)
Length = 287
Score = 34.3 bits (77), Expect = 0.23
Identities = 30/130 (23%), Positives = 55/130 (42%), Gaps = 9/130 (6%)
Query: 77 LRGEWTIWKQL-LGKETGLGWNHHIGNIDADSAWWDAKIRNVKYAKFRFQGLEFCDELEF 135
L + + W++L L + ++ NI +D W N+ ++ L F +L
Sbjct: 9 LSNDDSAWRELTLTAQDSDFFDRDTSNILSDFGW------NLHHSSDHPHSLRFDSDLTQ 62
Query: 136 IFG--EIVATSQCACAPAMGVPLESTGKNTTADVAREIIESDDELNIDELSPMENTQSKN 193
G TS C+ + A+ V + ST N +A + +++ E +P T K
Sbjct: 63 TTGVKPTTVTSSCSSSAAVSVAVTSTNNNPSATSSSSEDPAENSTASAEKTPPPETPVKE 122
Query: 194 KRKVSPRMRK 203
K+K R+R+
Sbjct: 123 KKKAQKRIRQ 132
>SC17_COPCI (P78603) Vesicular-fusion protein SEC17
Length = 285
Score = 33.9 bits (76), Expect = 0.30
Identities = 22/66 (33%), Positives = 30/66 (45%), Gaps = 12/66 (18%)
Query: 47 NKKWEEIRDEYNKRANKNYTQKQLKNRMDTLRGEWTIWKQLLGKETGLGWNHHIGNIDAD 106
N KWEE D Y + AN +KQ K D E ++ KE ++DA
Sbjct: 29 NSKWEEAGDLYQQAANAFKLEKQFKEAGDAFAREAECREKC--KE----------SLDAG 76
Query: 107 SAWWDA 112
+AWW+A
Sbjct: 77 NAWWNA 82
>S521_HUMAN (Q96MU7) Putative splicing factor YT521
Length = 727
Score = 31.6 bits (70), Expect = 1.5
Identities = 19/53 (35%), Positives = 26/53 (48%), Gaps = 1/53 (1%)
Query: 162 NTTADVAREIIESDDELNIDELSPMENTQSKNKRKVSPRMRKQQRVRQRLGLH 214
N D+ E+ E DDEL E +N + +KRK S RM RQ+ +H
Sbjct: 14 NVLDDILTEVPEQDDELYNPESEQDKNEKKGSKRK-SDRMESTDTKRQKPSVH 65
>S521_RAT (Q9QY02) Putative splicing factor YT521 (RA301-binding
protein)
Length = 738
Score = 31.2 bits (69), Expect = 1.9
Identities = 19/53 (35%), Positives = 26/53 (48%), Gaps = 1/53 (1%)
Query: 162 NTTADVAREIIESDDELNIDELSPMENTQSKNKRKVSPRMRKQQRVRQRLGLH 214
N D+ E+ E DDEL E +N + +KRK S RM RQ+ +H
Sbjct: 14 NVLDDILTEVPEQDDELYNPESEQDKNEKKGSKRK-SERMESIDTKRQKPSIH 65
>CHEA_RHOSH (Q53135) Chemotaxis protein cheA (EC 2.7.3.-)
Length = 686
Score = 31.2 bits (69), Expect = 1.9
Identities = 27/86 (31%), Positives = 38/86 (43%), Gaps = 5/86 (5%)
Query: 127 LEFCDELEFIFGEIVATSQCA--CAPAMGVPLESTGKNTTAD---VAREIIESDDELNID 181
L+ D L I GE+V C GVP S +N D +AREI ES +
Sbjct: 298 LDRVDRLINIVGELVINQAMLSQCVQDEGVPPRSPVRNRLDDFRNLAREIQESVMAIRAQ 357
Query: 182 ELSPMENTQSKNKRKVSPRMRKQQRV 207
+ P+ S+ R+ S +KQ R+
Sbjct: 358 AIKPLFQRMSRIAREASEISQKQIRL 383
>RNE_ECOLI (P21513) Ribonuclease E (EC 3.1.4.-) (RNase E)
Length = 1061
Score = 30.4 bits (67), Expect = 3.3
Identities = 17/50 (34%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
Query: 166 DVAREIIESDDELNIDELSPMENTQSKNKRKVSPRMRKQQRVRQRLGLHQ 215
D R+ + LN++E S E Q + R V PR RKQ+++ Q++ Q
Sbjct: 685 DDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPR-RKQRQLNQKVRYEQ 733
>DA82_YEAST (P21705) DAL82 protein
Length = 255
Score = 30.4 bits (67), Expect = 3.3
Identities = 14/44 (31%), Positives = 22/44 (49%), Gaps = 1/44 (2%)
Query: 36 HKCGKLGIAFRNKKWEEIRDEYNKRANKNYTQ-KQLKNRMDTLR 78
HK A+R W+ + DEYN++ Y Q + LK + L+
Sbjct: 18 HKPHLKPYAYRQDSWQRVLDEYNRQTGSRYRQSRTLKTKFRRLK 61
>SYNM_YEAST (P25345) Asparaginyl-tRNA synthetase, mitochondrial
precursor (EC 6.1.1.22) (Asparagine--tRNA ligase)
(AsnRS)
Length = 492
Score = 30.0 bits (66), Expect = 4.3
Identities = 15/47 (31%), Positives = 25/47 (52%)
Query: 3 SKESLPNSSENLGKAKWDTFSTKMLLDICMVEIHKCGKLGIAFRNKK 49
S + P +S GK + T ST++ L+I + + +C L FR +K
Sbjct: 196 STNTSPTASSYFGKPTYLTVSTQLHLEILALSLSRCWTLSPCFRAEK 242
>VGLB_HSV2S (P24994) Glycoprotein B precursor
Length = 885
Score = 29.6 bits (65), Expect = 5.6
Identities = 22/68 (32%), Positives = 36/68 (52%), Gaps = 8/68 (11%)
Query: 11 SENLGKAKWDTFSTKMLLDICMVEIHKCGKLGIAFRNKKWEEIRDEYNKRANKNYTQKQL 70
S LG+A T ST + L++ M+E H+ L + R +EI+D +YT+ Q
Sbjct: 641 SHQLGRADVTTVSTFINLNLTMLEDHEFVPLEVYTR----QEIKDS----GLLDYTEVQR 692
Query: 71 KNRMDTLR 78
+N++ LR
Sbjct: 693 RNQLHALR 700
>SPS_MAIZE (P31927) Sucrose-phosphate synthase (EC 2.4.1.14)
(UDP-glucose-fructose-phosphate glucosyltransferase)
Length = 1068
Score = 29.6 bits (65), Expect = 5.6
Identities = 14/42 (33%), Positives = 23/42 (54%)
Query: 157 ESTGKNTTADVAREIIESDDELNIDELSPMENTQSKNKRKVS 198
E + T D+A ++ E + I EL+P+E T+ K +R S
Sbjct: 121 EQVRREATEDLAEDLSEGEKGDTIGELAPVETTKKKFQRNFS 162
>ADEC_METKA (Q8TX47) Adenine deaminase (EC 3.5.4.2) (Adenase)
(Adenine aminase)
Length = 599
Score = 29.6 bits (65), Expect = 5.6
Identities = 14/48 (29%), Positives = 22/48 (45%)
Query: 147 ACAPAMGVPLESTGKNTTADVAREIIESDDELNIDELSPMENTQSKNK 194
+C PA+G P E+ T DVAR+ +++ EL + K
Sbjct: 170 SCVPALGPPYETVEGEITVDVARKFASHPMVVSVGELMDVAGVMEGEK 217
>SUS2_ARATH (Q00917) Sucrose synthase (EC 2.4.1.13) (Sucrose-UDP
glucosyltransferase)
Length = 805
Score = 29.3 bits (64), Expect = 7.3
Identities = 13/38 (34%), Positives = 20/38 (52%)
Query: 50 WEEIRDEYNKRANKNYTQKQLKNRMDTLRGEWTIWKQL 87
W +I + KR + YT K+ R+ TL G + WK +
Sbjct: 734 WVKISEGGLKRIYERYTWKKYSERLLTLAGVYAFWKHV 771
>FPG_MYCLE (O69470) Formamidopyrimidine-DNA glycosylase (EC
3.2.2.23) (FAPY-DNA glycosylase)
Length = 282
Score = 29.3 bits (64), Expect = 7.3
Identities = 21/67 (31%), Positives = 33/67 (48%), Gaps = 10/67 (14%)
Query: 54 RDEYNKRANKNYTQKQLKNRMDTLRGEWTIWKQLLGKETGLGWNHHIGNIDADSAWWDAK 113
RD ++ R + K L+ + L+ +QLL ++T G IGNI AD A W A+
Sbjct: 139 RDPFDPRFDVEAVVKVLRCKHSELK------RQLLDQQTVSG----IGNIYADEALWRAE 188
Query: 114 IRNVKYA 120
+ + A
Sbjct: 189 VHGARIA 195
>ENV_BIV27 (P19557) Env polyprotein precursor (Coat polyprotein)
[Contains: Coat protein GP62 (Surface protein); Coat
protein GP40 (Transmembrane glycoprotein)]
Length = 904
Score = 29.3 bits (64), Expect = 7.3
Identities = 20/68 (29%), Positives = 35/68 (51%), Gaps = 7/68 (10%)
Query: 44 AFRNKKWEEIRDEYNKRANKNYTQKQLKNRMDTLRGEWTIWKQLLGKETGLGWNHHIGNI 103
++ +K W +++DEY+K +K LK R+D L + + G ET + ++H +
Sbjct: 665 SWNSKTWNDLQDEYDK-----IEEKILKIRVDWLNSSLSDTQDTFGLETSI-FDHLVQLF 718
Query: 104 DADSAWWD 111
D S W D
Sbjct: 719 DWTS-WKD 725
>ENV_BIV06 (P19556) Env polyprotein precursor (Coat polyprotein)
[Contains: Coat protein GP62 (Surface protein); Coat
protein GP40 (Transmembrane glycoprotein)]
Length = 875
Score = 29.3 bits (64), Expect = 7.3
Identities = 20/68 (29%), Positives = 35/68 (51%), Gaps = 7/68 (10%)
Query: 44 AFRNKKWEEIRDEYNKRANKNYTQKQLKNRMDTLRGEWTIWKQLLGKETGLGWNHHIGNI 103
++ +K W +++DEY+K +K LK R+D L + + G ET + ++H +
Sbjct: 636 SWNSKTWSDLQDEYDK-----IEEKILKIRVDWLNSSLSDTQDTFGLETSI-FDHLVQLF 689
Query: 104 DADSAWWD 111
D S W D
Sbjct: 690 DWTS-WKD 696
>CHS1_YEAST (P08004) Chitin synthase 1 (EC 2.4.1.16) (Chitin-UDP
acetyl-glucosaminyl transferase 1)
Length = 1131
Score = 29.3 bits (64), Expect = 7.3
Identities = 17/57 (29%), Positives = 28/57 (48%)
Query: 15 GKAKWDTFSTKMLLDICMVEIHKCGKLGIAFRNKKWEEIRDEYNKRANKNYTQKQLK 71
G +D+ +K LLD L F+ +++ + E N+ A KN+T +QLK
Sbjct: 383 GNFVFDSPISKTLLDQYATTTENANTLPNEFKFMRYQAVTCEPNQLAEKNFTVRQLK 439
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.317 0.132 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,335,646
Number of Sequences: 164201
Number of extensions: 1030692
Number of successful extensions: 2953
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 2941
Number of HSP's gapped (non-prelim): 18
length of query: 216
length of database: 59,974,054
effective HSP length: 106
effective length of query: 110
effective length of database: 42,568,748
effective search space: 4682562280
effective search space used: 4682562280
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 63 (28.9 bits)
Medicago: description of AC140104.6