BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC139344.7 + phase: 1 /pseudo
(478 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PMTX_SCHPO (O13898) Probable dolichyl-phosphate-mannose--protein... 33 2.1
MACF_HUMAN (Q9UPN3) Microtubule-actin crosslinking factor 1, iso... 32 2.8
MAC4_HUMAN (Q96PK2) Microtubule-actin crosslinking factor 1, iso... 32 2.8
IL8A_RABIT (P21109) High affinity interleukin-8 receptor A (IL-8... 32 3.6
EFTS_BUCAI (P57326) Elongation factor Ts (EF-Ts) 32 4.7
TOP2_DROME (P15348) DNA topoisomerase II (EC 5.99.1.3) 31 6.1
GUAC_STRPY (Q99ZQ1) GMP reductase (EC 1.7.1.7) (Guanosine 5'-mon... 31 6.1
GUAC_STRP8 (Q8P129) GMP reductase (EC 1.7.1.7) (Guanosine 5'-mon... 31 6.1
GUAC_STRP3 (Q8K7I6) GMP reductase (EC 1.7.1.7) (Guanosine 5'-mon... 31 6.1
CLPX_LACLA (Q9CGE6) ATP-dependent Clp protease ATP-binding subun... 31 6.1
VP10_RGDV (P29078) Nonstructural protein Pns10 31 8.0
CLPX_LACLC (Q8GJP6) ATP-dependent Clp protease ATP-binding subun... 31 8.0
>PMTX_SCHPO (O13898) Probable dolichyl-phosphate-mannose--protein
mannosyltransferase C22A12.07c (EC 2.4.1.109)
Length = 893
Score = 32.7 bits (73), Expect = 2.1
Identities = 36/141 (25%), Positives = 55/141 (38%), Gaps = 21/141 (14%)
Query: 205 LADEENKNIVENLIWEYYRDIYFGHRKVVMDLKGKEDELLTNFEKTAESAFLMVVVFALS 264
L E+K IV N W+ Y G FE A F + ++ S
Sbjct: 394 LHSHEHKPIVSNNDWQLEASAYGGF----------------GFEGDANDLFRIQILEKKS 437
Query: 265 VTKHKLSSTFAQEIQTEISLKILVSLSCVEYFRHVRLPEYMETIRKVTAIVKKNENACTF 324
KH S+ + + T+ L I V +C H R P++ + R+VT E + T+
Sbjct: 438 --KHATSNGTVETLNTKFRL-IHVFANCELMSSHRRFPDWGDYQREVTCCRNCVERSTTW 494
Query: 325 FVNSIPSYGDLTNGPDQKTKY 345
F+ S +Y D +K Y
Sbjct: 495 FIES--NYHDGLPSDSRKITY 513
>MACF_HUMAN (Q9UPN3) Microtubule-actin crosslinking factor 1, isoforms
1/2/3 (Actin cross-linking family protein 7) (Macrophin
1) (Trabeculin-alpha) (620 kDa actin-binding protein)
(ABP620)
Length = 5430
Score = 32.3 bits (72), Expect = 2.8
Identities = 25/90 (27%), Positives = 42/90 (45%), Gaps = 4/90 (4%)
Query: 242 ELLTNFEKTAESAFLMVVVFALSVTKHKLSSTFAQEIQTEISLKILVSLSCVEYFRHV-- 299
E++T F +TA+S V+ L+ + Q+ + E SLK L L E F H+
Sbjct: 2107 EMVTRFMETADSTTAAVLQGKLAEVSQRFEQLCLQQQEKESSLKKL--LPQAEMFEHLSG 2164
Query: 300 RLPEYMETIRKVTAIVKKNENACTFFVNSI 329
+L ++ME ++ A + + T F I
Sbjct: 2165 KLQQFMENKSRMLASGNQPDQDITHFFQQI 2194
>MAC4_HUMAN (Q96PK2) Microtubule-actin crosslinking factor 1, isoform
4
Length = 5938
Score = 32.3 bits (72), Expect = 2.8
Identities = 25/90 (27%), Positives = 42/90 (45%), Gaps = 4/90 (4%)
Query: 242 ELLTNFEKTAESAFLMVVVFALSVTKHKLSSTFAQEIQTEISLKILVSLSCVEYFRHV-- 299
E++T F +TA+S V+ L+ + Q+ + E SLK L L E F H+
Sbjct: 2609 EMVTRFMETADSTTAAVLQGKLAEVSQRFEQLCLQQQEKESSLKKL--LPQAEMFEHLSG 2666
Query: 300 RLPEYMETIRKVTAIVKKNENACTFFVNSI 329
+L ++ME ++ A + + T F I
Sbjct: 2667 KLQQFMENKSRMLASGNQPDQDITHFFQQI 2696
>IL8A_RABIT (P21109) High affinity interleukin-8 receptor A (IL-8R
A) (CXCR-1)
Length = 355
Score = 32.0 bits (71), Expect = 3.6
Identities = 21/59 (35%), Positives = 32/59 (53%), Gaps = 8/59 (13%)
Query: 122 VLLQCVTLGM-------TRTISFSSHSSLFVCLGLSLLTRILPLP-RLYESVFELSPSS 172
+LL C+++ TRT++ H F+CLG+ L+ IL LP L+ VF + SS
Sbjct: 131 LLLACISVDRYLAIVHATRTLTQKRHLVKFICLGIWALSLILSLPFFLFRQVFSPNNSS 189
>EFTS_BUCAI (P57326) Elongation factor Ts (EF-Ts)
Length = 268
Score = 31.6 bits (70), Expect = 4.7
Identities = 36/165 (21%), Positives = 65/165 (38%), Gaps = 22/165 (13%)
Query: 64 SGIGVLRVTDRYASSTGMKVDVLTRMRTSAIVRVEALVSDLVSRTLRFRNLGNDLQDRVL 123
+G G L G + +R S + E ++++ ++ F + N++ +
Sbjct: 17 TGAGFLACKRALLEENGDIESAIDNLRKSGKLTAEKKINNITNQGAIFSKIKNNIGVMLE 76
Query: 124 LQCVTLGMTRTISFSSHSSLFVCLGLSLLTRIL--------PLPRLYESVFELSPSSGGL 175
L C T F S +LF+CLG +L L L ++ES S G
Sbjct: 77 LNCET-------DFVSKDNLFICLGEDILVEALEKRIKDINQLKVIFESRRTELVSKVGE 129
Query: 176 KVNEIKEH---PDNILFKEAGAVTGIFCNLYVLADEENKNIVENL 217
+N + H +NI + G+ + V + NK I++N+
Sbjct: 130 NINIRRFHLIEGENIF----SYLHGVRIGVLVSSSSLNKTILKNI 170
>TOP2_DROME (P15348) DNA topoisomerase II (EC 5.99.1.3)
Length = 1447
Score = 31.2 bits (69), Expect = 6.1
Identities = 45/195 (23%), Positives = 77/195 (39%), Gaps = 28/195 (14%)
Query: 172 SGGLKVNEIKEHPDNILFKEAGAVTGIFCNLYVLADEENKNIVENLIWEYYRDIYFGHRK 231
+GG K ++IK P EAG I C L + + K++ + + RD+Y
Sbjct: 407 TGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVIGRDLY----- 461
Query: 232 VVMDLKGKEDELL-TNFEKTAESAFL--MVVVFALSVTKHKLSS------------TFAQ 276
V L+GK + NF++ +E+A + + + L K L+
Sbjct: 462 GVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTD 521
Query: 277 EIQTEISLK-ILVSLSCVEYFRHVRLPEYMETIRKVTAIVKKNENACTFFVNSIPSY--- 332
+ Q +K +L++ + +RLP E I + KKNE + S+P +
Sbjct: 522 QDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFY---SLPEFEEW 578
Query: 333 -GDLTNGPDQKTKYF 346
D N KY+
Sbjct: 579 KNDTANHHTYNIKYY 593
>GUAC_STRPY (Q99ZQ1) GMP reductase (EC 1.7.1.7) (Guanosine
5'-monophosphate oxidoreductase) (Guanosine
monophosphate reductase)
Length = 327
Score = 31.2 bits (69), Expect = 6.1
Identities = 27/108 (25%), Positives = 46/108 (42%), Gaps = 14/108 (12%)
Query: 361 LRVIPTLIECLRGPVFGDMVAPTMFLYMEHPNGK------VARASHSVFTAFISMGK--- 411
L VIP ++ + + +A + Y+ H + + R A IS+G
Sbjct: 41 LPVIPANMQTIIDETIAEQLAKEGYFYIMHRFDEDSRKPFIKRMHEQGLIASISVGVKAC 100
Query: 412 ESEKIDSLIEGEARFLSLSIIILPNTLGMASGVVGMVRHLPAGSPTTF 459
E E + SL E F+++ I G A+ V+ M++H+ P TF
Sbjct: 101 EYEFVTSLKEDAPEFITIDI-----AHGHANSVIDMIKHIKTELPETF 143
>GUAC_STRP8 (Q8P129) GMP reductase (EC 1.7.1.7) (Guanosine
5'-monophosphate oxidoreductase) (Guanosine
monophosphate reductase)
Length = 327
Score = 31.2 bits (69), Expect = 6.1
Identities = 27/108 (25%), Positives = 46/108 (42%), Gaps = 14/108 (12%)
Query: 361 LRVIPTLIECLRGPVFGDMVAPTMFLYMEHPNGK------VARASHSVFTAFISMGK--- 411
L VIP ++ + + +A + Y+ H + + R A IS+G
Sbjct: 41 LPVIPANMQTIIDETIAEQLAKEGYFYIMHRFDEDSRKPFIKRMHEQGLIASISVGVKAY 100
Query: 412 ESEKIDSLIEGEARFLSLSIIILPNTLGMASGVVGMVRHLPAGSPTTF 459
E E + SL E F+++ I G A+ V+ M++H+ P TF
Sbjct: 101 EYEFVTSLKEDTPEFITIDI-----AHGHANSVIDMIKHIKTELPETF 143
>GUAC_STRP3 (Q8K7I6) GMP reductase (EC 1.7.1.7) (Guanosine
5'-monophosphate oxidoreductase) (Guanosine
monophosphate reductase)
Length = 327
Score = 31.2 bits (69), Expect = 6.1
Identities = 27/108 (25%), Positives = 46/108 (42%), Gaps = 14/108 (12%)
Query: 361 LRVIPTLIECLRGPVFGDMVAPTMFLYMEHPNGK------VARASHSVFTAFISMGK--- 411
L VIP ++ + + +A + Y+ H + + R A IS+G
Sbjct: 41 LPVIPANMQTIIDETIAEQLAKEGYFYIMHRFDEDSRKPFIKRMHEQGLIASISVGVKAC 100
Query: 412 ESEKIDSLIEGEARFLSLSIIILPNTLGMASGVVGMVRHLPAGSPTTF 459
E E + SL E F+++ I G A+ V+ M++H+ P TF
Sbjct: 101 EYEFVTSLKEDAPEFITIDI-----AHGHANSVIDMIKHIKTELPETF 143
>CLPX_LACLA (Q9CGE6) ATP-dependent Clp protease ATP-binding subunit
clpX
Length = 411
Score = 31.2 bits (69), Expect = 6.1
Identities = 29/127 (22%), Positives = 54/127 (41%), Gaps = 11/127 (8%)
Query: 201 NLYVLADEENKNIVENLIWEYYRDIYFGHRKVVMDLKGKEDELLTNFEKTAESAFL---- 256
N YV+ E+ K + ++ +Y+ I F K+ D++ ++ +L + FL
Sbjct: 70 NEYVIGQEKAKRALAVAVYNHYKRINFAASKIAEDIELQKSNILLIGPTGSGKTFLAQTL 129
Query: 257 ---MVVVFALSVTKHKLSSTFAQEIQTEISLKILVSLSCVEYFRHVRLPEYMETIRKVTA 313
+ V FA++ + + E I LK+L + F R + I ++
Sbjct: 130 AKSLNVPFAIADATSLTEAGYVGEDVENILLKLLQASD----FNIERAERGIIYIDEIDK 185
Query: 314 IVKKNEN 320
I KK+EN
Sbjct: 186 IAKKSEN 192
>VP10_RGDV (P29078) Nonstructural protein Pns10
Length = 320
Score = 30.8 bits (68), Expect = 8.0
Identities = 26/119 (21%), Positives = 51/119 (42%), Gaps = 8/119 (6%)
Query: 167 ELSPSSGGLKVNEIKEHPDNILFKEAGAVTGIFCNLYVLADEENKNIVENLIWEYYRDIY 226
+L +SGG +N+ + ++F A NLYV + N + EY D++
Sbjct: 181 KLPDASGGTLLNQKSKEQMRVIFSNAKVRIANSINLYVTTHTNSYNEYVREVAEYVADLW 240
Query: 227 FGHRKVVMDLKGKEDELLTNFEKTAESAFLMVVVFALSVTKHKLSSTFAQEIQTEISLK 285
+ + + +G E+EL AE ++ ++ TK +L + + E+ L+
Sbjct: 241 --NIQTTTNTQGHENEL------AAEDFGVLASSSQMNGTKSELGDSVIKSDGNEVKLE 291
>CLPX_LACLC (Q8GJP6) ATP-dependent Clp protease ATP-binding subunit
clpX
Length = 411
Score = 30.8 bits (68), Expect = 8.0
Identities = 35/151 (23%), Positives = 65/151 (42%), Gaps = 14/151 (9%)
Query: 179 EIKEHPDNILFKEAGAVTGIFCNL--YVLADEENKNIVENLIWEYYRDIYFGHRKVVMDL 236
E+KE D+ + E +F +L YV+ E+ K + ++ +Y+ I F K+ D+
Sbjct: 47 ELKEEQDSEML-EVKTPKEMFDHLNEYVIGQEKAKRALAVAVYNHYKRINFTASKIAEDI 105
Query: 237 KGKEDELLTNFEKTAESAFL-------MVVVFALSVTKHKLSSTFAQEIQTEISLKILVS 289
+ ++ +L + FL + V FA++ + + E I LK+L +
Sbjct: 106 ELQKSNILLIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENILLKLLQA 165
Query: 290 LSCVEYFRHVRLPEYMETIRKVTAIVKKNEN 320
F R + I ++ I KK+EN
Sbjct: 166 SD----FNIERAERGIIYIDEIDKIAKKSEN 192
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.326 0.140 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52,673,289
Number of Sequences: 164201
Number of extensions: 2118987
Number of successful extensions: 5905
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 5905
Number of HSP's gapped (non-prelim): 12
length of query: 478
length of database: 59,974,054
effective HSP length: 114
effective length of query: 364
effective length of database: 41,255,140
effective search space: 15016870960
effective search space used: 15016870960
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 68 (30.8 bits)
Medicago: description of AC139344.7