BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136450.2 + phase: 0 /pseudo
(198 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YO87_CAEEL (P34623) Hypothetical protein ZK1236.7 in chromosome III 35 0.086
RF1_BUCBP (P59456) Peptide chain release factor 1 (RF-1) 33 0.33
EX70_DROME (Q9VSJ8) 70 kDa exocyst complex protein 33 0.43
Y4LL_RHISN (P55552) Hypothetical 91.8 kDa protein y4lL 31 2.1
RM50_YEAST (P53724) 60S ribosomal protein L50, mitochondrial pre... 31 2.1
CN7B_SCHPO (Q09722) COP9/signalosome complex subunit 7B 31 2.1
YG3A_YEAST (P53278) Hypothetical 92.7 kDa protein in ASN2-PHB1 i... 29 6.2
SPCB_HUMAN (P11277) Spectrin beta chain, erythrocyte (Beta-I spe... 29 6.2
YHES_ECOLI (P63389) Hypothetical ABC transporter ATP-binding pro... 29 8.1
YHES_ECO57 (P63390) Hypothetical ABC transporter ATP-binding pro... 29 8.1
NCR2_HUMAN (Q9Y618) Nuclear receptor corepressor 2 (N-CoR2) (Sil... 29 8.1
DESR_DESBR (Q46495) Desulfoferrodoxin (EC 1.15.-.-) (Dfx) (Super... 29 8.1
ARLY_OCEIH (Q8ELT9) Argininosuccinate lyase (EC 4.3.2.1) (Argino... 29 8.1
>YO87_CAEEL (P34623) Hypothetical protein ZK1236.7 in chromosome III
Length = 324
Score = 35.4 bits (80), Expect = 0.086
Identities = 35/118 (29%), Positives = 53/118 (44%), Gaps = 7/118 (5%)
Query: 29 FYNHVMSLDEELWDILEGGIGDLVVDEEGADVDRRKHTPAQKKLYK---KHHTIRGALVK 85
F NH+M+ E++ D L+GG DE+G + +RK Q K K + + +R +
Sbjct: 101 FVNHMMNDGEDVED-LDGGAEQFEYDEDGKKIGKRKAAKLQAKEEKRQMREYEVREREER 159
Query: 86 AIPKAEYMKMSDKSTAKSMFASLCANYEGSKKVREAKALMLVHQYELFKMKDGESIEQ 143
+ E K D+ AK A A E +K RE K +Y MK +IE+
Sbjct: 160 KRREEEREKKRDEERAKEE-ADEKAEEERLRKEREEKERKEHEEY--LAMKASFAIEE 214
>RF1_BUCBP (P59456) Peptide chain release factor 1 (RF-1)
Length = 362
Score = 33.5 bits (75), Expect = 0.33
Identities = 28/94 (29%), Positives = 42/94 (43%), Gaps = 14/94 (14%)
Query: 15 FNGDL-------EEFS*WKTNFYNHVMSLDEELWDIL-----EGGIGDLVVDEEGADVDR 62
F+GDL EF WKTN + SL DI+ +G G L + G V R
Sbjct: 127 FSGDLFRMYIKYSEFQNWKTNIIHMTHSLKGGYKDIIVKITGKGSYGKLKFESGGHRVQR 186
Query: 63 RKHTPAQKKLYKKHHTIRGALVKAIPKAEYMKMS 96
T +Q +++ T A++ +PK E K++
Sbjct: 187 VPKTESQGRVHTS--TCIVAVIPVVPKKEIEKVN 218
>EX70_DROME (Q9VSJ8) 70 kDa exocyst complex protein
Length = 693
Score = 33.1 bits (74), Expect = 0.43
Identities = 20/72 (27%), Positives = 37/72 (50%)
Query: 88 PKAEYMKMSDKSTAKSMFASLCANYEGSKKVREAKALMLVHQYELFKMKDGESIEQMYSR 147
P A +K D+S K F++ ++E + K++ ++ V E K + E I +Y+R
Sbjct: 597 PVAGKVKDKDRSVLKERFSNFNKDFEEACKIQRGISIPDVILREGIKRDNVEHILPIYNR 656
Query: 148 FHTLVSGLQILK 159
F+ + SG+ K
Sbjct: 657 FYEIYSGVHFSK 668
>Y4LL_RHISN (P55552) Hypothetical 91.8 kDa protein y4lL
Length = 827
Score = 30.8 bits (68), Expect = 2.1
Identities = 17/70 (24%), Positives = 34/70 (48%), Gaps = 7/70 (10%)
Query: 59 DVDRRKHTPAQKKLYKK--HHTIRGALVKAIP-----KAEYMKMSDKSTAKSMFASLCAN 111
D+ R++ ++ L +K HH R + +P + E+ ++SD + A LC +
Sbjct: 377 DITERRNAEQERLLAQKQIHHMARHDALTGLPNRQFLREEFERLSDHIAPSTRLAILCLD 436
Query: 112 YEGSKKVREA 121
+G K + +A
Sbjct: 437 LDGFKAINDA 446
>RM50_YEAST (P53724) 60S ribosomal protein L50, mitochondrial
precursor
Length = 139
Score = 30.8 bits (68), Expect = 2.1
Identities = 22/95 (23%), Positives = 43/95 (45%), Gaps = 3/95 (3%)
Query: 72 LYKKHHTIRGALVKAIPKAEYMKMSDKSTAKSMFASLCANYEGSKKVREAKALM---LVH 128
L K+ H ++ ++K P+ + K + S+ + N+ G+K + + + L+
Sbjct: 12 LTKRTHRVKVQVLKDFPRFQLYKGQVANVKPSLMRNYLHNFNGAKYILSEEHDINTELLK 71
Query: 129 QYELFKMKDGESIEQMYSRFHTLVSGLQILKKSCV 163
QY+ + K E +Q+ R T V L+K V
Sbjct: 72 QYQTLEAKLEEDHQQLSKRHETEVQKNMELRKESV 106
>CN7B_SCHPO (Q09722) COP9/signalosome complex subunit 7B
Length = 402
Score = 30.8 bits (68), Expect = 2.1
Identities = 23/86 (26%), Positives = 42/86 (48%), Gaps = 8/86 (9%)
Query: 90 AEYMKMSDKSTAKSMFASLCANYEGSKKVREAKALMLVHQYELFKMKDGESIEQMYSRFH 149
A Y+ + +T K++ A LC Y S+ V+E L L + L + +E + + F
Sbjct: 22 AMYLDNLEANTDKNVLA-LCREYLASENVKEVLNLFLT-RLPLLAQAPEKELEPILAVF- 78
Query: 150 TLVSGLQILKKSCVASDHVSKILRSL 175
+ ++++S DHVSK ++L
Sbjct: 79 -----INLIQESAAFEDHVSKFCQAL 99
>YG3A_YEAST (P53278) Hypothetical 92.7 kDa protein in ASN2-PHB1
intergenic region
Length = 816
Score = 29.3 bits (64), Expect = 6.2
Identities = 23/96 (23%), Positives = 46/96 (46%), Gaps = 18/96 (18%)
Query: 3 NNKKSDFGKVPKFNGDLEEFS*WKTNFYNHVMSLDEELWDILEGGIGDLVVDEEGADVDR 62
NN+K++ K EF+ W TN N LD +++ I + + V E ++++
Sbjct: 541 NNEKTNVQK---------EFNDWTTNLSNLSQQLDAQIFKINQINLKQGKVQNEIDNLEK 591
Query: 63 RK-----HTPAQKKLYKKHHTIRGALVKAIPKAEYM 93
+K T KKL++K+ + ++++ EY+
Sbjct: 592 KKEDLVTQTEENKKLHEKNVQV----LESVENKEYL 623
>SPCB_HUMAN (P11277) Spectrin beta chain, erythrocyte (Beta-I
spectrin)
Length = 2137
Score = 29.3 bits (64), Expect = 6.2
Identities = 31/125 (24%), Positives = 53/125 (41%), Gaps = 20/125 (16%)
Query: 37 DEELWDILEGGIGDLVVDEEGADVDRRKHTPAQKKLYKKHHTIR-------GALVKAIPK 89
++EL+ I + ++ DEEGA V ++H Q+ + I+ G L P+
Sbjct: 1602 EQELYVISD----EIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPE 1657
Query: 90 AEYMKMSDKSTAKSMFASLCANYEGSKKVREAKALMLVHQYELFKMK-DGESIEQMYSRF 148
E + K +Y G K V E + L + Y LF++K + + +EQ S
Sbjct: 1658 GEQIIRLQGQVDK--------HYAGLKDVAEERKRKLENMYHLFQLKRETDDLEQWISEK 1709
Query: 149 HTLVS 153
+ S
Sbjct: 1710 ELVAS 1714
>YHES_ECOLI (P63389) Hypothetical ABC transporter ATP-binding
protein yheS
Length = 637
Score = 28.9 bits (63), Expect = 8.1
Identities = 29/129 (22%), Positives = 52/129 (39%), Gaps = 6/129 (4%)
Query: 11 KVPKFNGDLEEFS*WKTNFYNHVMSLDEELWDILEGGIGDLVVDEEGADVDRRKHT-PAQ 69
KV F+GDLE++ W ++ DE + D++ + + R T P +
Sbjct: 504 KVEPFDGDLEDYQQWLSDVQKQENQTDEAPKE--NANSAQARKDQKRREAELRAQTQPLR 561
Query: 70 K---KLYKKHHTIRGALVKAIPKAEYMKMSDKSTAKSMFASLCANYEGSKKVREAKALML 126
K +L K+ + L +A K ++ D+S + A L + E + L
Sbjct: 562 KEIARLEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWL 621
Query: 127 VHQYELFKM 135
Q +L +M
Sbjct: 622 EAQEQLEQM 630
>YHES_ECO57 (P63390) Hypothetical ABC transporter ATP-binding
protein yheS
Length = 637
Score = 28.9 bits (63), Expect = 8.1
Identities = 29/129 (22%), Positives = 52/129 (39%), Gaps = 6/129 (4%)
Query: 11 KVPKFNGDLEEFS*WKTNFYNHVMSLDEELWDILEGGIGDLVVDEEGADVDRRKHT-PAQ 69
KV F+GDLE++ W ++ DE + D++ + + R T P +
Sbjct: 504 KVEPFDGDLEDYQQWLSDVQKQENQTDEAPKE--NANSAQARKDQKRREAELRAQTQPLR 561
Query: 70 K---KLYKKHHTIRGALVKAIPKAEYMKMSDKSTAKSMFASLCANYEGSKKVREAKALML 126
K +L K+ + L +A K ++ D+S + A L + E + L
Sbjct: 562 KEIARLEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWL 621
Query: 127 VHQYELFKM 135
Q +L +M
Sbjct: 622 EAQEQLEQM 630
>NCR2_HUMAN (Q9Y618) Nuclear receptor corepressor 2 (N-CoR2)
(Silencing mediator of retinoic acid and thyroid hormone
receptor) (SMRT) (SMRTe) (Thyroid-,
retinoic-acid-receptor-associated corepressor) (T3
receptor-associating factor) (TRAC) (CTG repeat pr
Length = 2517
Score = 28.9 bits (63), Expect = 8.1
Identities = 15/64 (23%), Positives = 31/64 (48%), Gaps = 8/64 (12%)
Query: 32 HVMSLDEELWDILEGGIGDLVVDEE-----GADVDRRKHTPAQKKLYKKHHTIRGALVKA 86
H + + +D++EG +G + G + +H+P K+ H IRG++ +
Sbjct: 1302 HETAAPKRTYDMMEGRVGRAISSASIEGLMGRAIPPERHSPHH---LKEQHHIRGSITQG 1358
Query: 87 IPKA 90
IP++
Sbjct: 1359 IPRS 1362
>DESR_DESBR (Q46495) Desulfoferrodoxin (EC 1.15.-.-) (Dfx)
(Superoxide reductase) (SOR)
Length = 126
Score = 28.9 bits (63), Expect = 8.1
Identities = 16/61 (26%), Positives = 29/61 (47%), Gaps = 8/61 (13%)
Query: 42 DILEGGIGDLV--------VDEEGADVDRRKHTPAQKKLYKKHHTIRGALVKAIPKAEYM 93
++L GGIG+LV + E D + KH P +K+ + GA+ + + Y+
Sbjct: 18 EVLNGGIGELVCCNQDMKLMSENTVDAAKEKHVPVIEKIDGGYKVKVGAVAHPMEEKHYI 77
Query: 94 K 94
+
Sbjct: 78 Q 78
>ARLY_OCEIH (Q8ELT9) Argininosuccinate lyase (EC 4.3.2.1)
(Arginosuccinase) (ASAL)
Length = 459
Score = 28.9 bits (63), Expect = 8.1
Identities = 25/92 (27%), Positives = 40/92 (43%), Gaps = 15/92 (16%)
Query: 14 KFNGDLEEFS*WKTN-FYNHVMSLDEELWDILEGGIGDLVVDEEGADVDRRKHTPAQKKL 72
K N D+ E KT Y H+M + L + D+ D+EG +
Sbjct: 282 KKNPDVAELVRGKTGRVYGHLMGMLTTLKGLPLAYNKDMQEDKEG--------------M 327
Query: 73 YKKHHTIRGALVKAIPKAEYMKMSDKSTAKSM 104
+ H T++GAL P E MK++ +S K++
Sbjct: 328 FDAHETLKGALQLFAPMMESMKVNKESMYKAV 359
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.134 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,835,235
Number of Sequences: 164201
Number of extensions: 914479
Number of successful extensions: 2021
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 2019
Number of HSP's gapped (non-prelim): 13
length of query: 198
length of database: 59,974,054
effective HSP length: 105
effective length of query: 93
effective length of database: 42,732,949
effective search space: 3974164257
effective search space used: 3974164257
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 63 (28.9 bits)
Medicago: description of AC136450.2