BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC134322.4 - phase: 0 /pseudo
(1735 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MC50_ARATH (P92555) Hypothetical mitochondrial protein AtMg01250... 79 1e-13
POL2_MOUSE (P11369) Retrovirus-related Pol polyprotein [Contains... 54 3e-06
PO23_POPJA (Q05118) Retrovirus-related Pol polyprotein from type... 47 5e-04
LIN1_NYCCO (P08548) LINE-1 reverse transcriptase homolog 46 0.001
YTX2_XENLA (P14381) Transposon TX1 hypothetical 149 kDa protein ... 44 0.003
PO21_NASVI (Q03278) Retrovirus-related Pol polyprotein from type... 42 0.015
LIN1_HUMAN (P08547) LINE-1 reverse transcriptase homolog 41 0.033
POLR_DROME (P16423) Retrovirus-related Pol polyprotein from type... 37 0.48
PO22_POPJA (Q03274) Retrovirus-related Pol polyprotein from type... 37 0.48
YO84_CAEEL (P34620) Hypothetical protein ZK1236.4 in chromosome III 35 1.4
YKI3_YEAST (P36079) Hypothetical 23.7 kDa protein in MDH1-VMA5 i... 35 1.8
PO21_SCICO (Q03279) Retrovirus-related Pol polyprotein from type... 34 4.0
YIK0_YEAST (P40490) Hypothetical 13.5 kDa protein in MOB1-SGA1 i... 33 6.9
>MC50_ARATH (P92555) Hypothetical mitochondrial protein AtMg01250
(ORF102)
Length = 122
Score = 78.6 bits (192), Expect = 1e-13
Identities = 39/66 (59%), Positives = 46/66 (69%)
Query: 1423 ILNKEKIGPIIPGFGLRQGDPLSPYLFILCAEGLSAQIRNAESRGDLQGIRVCRIAPRVS 1482
I+N G + P GLRQGDPLSPYLFILC E LS R A+ +G L GIRV +PR++
Sbjct: 13 IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72
Query: 1483 HLLFAD 1488
HLLFAD
Sbjct: 73 HLLFAD 78
>POL2_MOUSE (P11369) Retrovirus-related Pol polyprotein [Contains:
Reverse transcriptase (EC 2.7.7.49); Endonuclease]
Length = 1300
Score = 54.3 bits (129), Expect = 3e-06
Identities = 38/127 (29%), Positives = 67/127 (51%), Gaps = 5/127 (3%)
Query: 1358 AIELVHYMMTKMKGKEKSVALKLDISKAYDRIDWAYLRDVMSKMGFSEK*I*WIMMWVGT 1417
+I ++HY+ K+K K + + LD KA+D+I ++ V+ + G + I
Sbjct: 605 SINVIHYI-NKLKDKNHMI-ISLDAEKAFDKIQHPFMIKVLERSGIQGPYLNMIKAIYSK 662
Query: 1418 VDYSVILNKEKIGPIIPGFGLRQGDPLSPYLFILCAEGLSAQIRNAESRGDLQGIRVCRI 1477
++ +N EK+ I G RQG PLSPYLF + E L+ IR + +++GI++ +
Sbjct: 663 PVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLARAIRQQK---EIKGIQIGKE 719
Query: 1478 APRVSHL 1484
++S L
Sbjct: 720 EVKISLL 726
>PO23_POPJA (Q05118) Retrovirus-related Pol polyprotein from type I
retrotransposable element R2 [Contains: Reverse
transcriptase (EC 2.7.7.49); Endonuclease] (Fragment)
Length = 606
Score = 47.0 bits (110), Expect = 5e-04
Identities = 37/131 (28%), Positives = 63/131 (47%), Gaps = 8/131 (6%)
Query: 1359 IELVHYM-MTKMKGKEKSVALKLDISKAYDRIDWAYLRDVMSKMGFSEK*I*WIMMWVGT 1417
I L HY+ + ++KGK +V + LDI KA+D + + M G + +IM +
Sbjct: 7 IMLEHYIKLRRLKGKTYNV-VSLDIRKAFDTVSHPAILRAMRAFGIDDGMQDFIMSTITD 65
Query: 1418 VDYSVILNKEKIGPIIPGFGLRQGDPLSPYLFILCAEGLSAQIRNAESRGDLQGIRVCRI 1477
++++ I G++QGDPLSP LF + + L ++ + + + C+I
Sbjct: 66 AYTNIVVGGRTTNKIYIRNGVKQGDPLSPVLFNIVLDELVTRLNDEQPGASM--TPACKI 123
Query: 1478 APRVSHLLFAD 1488
A L FAD
Sbjct: 124 A----SLAFAD 130
>LIN1_NYCCO (P08548) LINE-1 reverse transcriptase homolog
Length = 1260
Score = 45.8 bits (107), Expect = 0.001
Identities = 37/123 (30%), Positives = 60/123 (48%), Gaps = 6/123 (4%)
Query: 1366 MTKMKGKEKSVALKLDISKAYDRIDWAYLRDVMSKMGFSEK*I*WIMMWVGTVDYSVILN 1425
+ K+K K+ + L +D KA+D I ++ + K+G + I ++ILN
Sbjct: 585 INKLKNKDHMI-LSIDAEKAFDNIQHPFMIRTLKKIGIEGTFLKLIEAIYSKPTANIILN 643
Query: 1426 KEKIGPIIPGFGLRQGDPLSPYLFILCAEGLSAQIRNAESRGDLQGIRVCRIAPRVSHLL 1485
K+ G RQG PLSP LF + E L+ IR ++ ++GI + ++S L
Sbjct: 644 GVKLKSFPLRSGTRQGCPLSPLLFNIVMEVLAIAIREEKA---IKGIHIGSEEIKLS--L 698
Query: 1486 FAD 1488
FAD
Sbjct: 699 FAD 701
>YTX2_XENLA (P14381) Transposon TX1 hypothetical 149 kDa protein (ORF
2)
Length = 1308
Score = 44.3 bits (103), Expect = 0.003
Identities = 26/84 (30%), Positives = 40/84 (46%)
Query: 1378 LKLDISKAYDRIDWAYLRDVMSKMGFSEK*I*WIMMWVGTVDYSVILNKEKIGPIIPGFG 1437
L LD KA+DR+D YL + F + + ++ + + V +N P+ G G
Sbjct: 591 LSLDQEKAFDRVDHQYLIGTLQAYSFGPQFVGYLKTMYASAECLVKINWSLTAPLAFGRG 650
Query: 1438 LRQGDPLSPYLFILCAEGLSAQIR 1461
+RQG PLS L+ L E +R
Sbjct: 651 VRQGCPLSGQLYSLAIEPFLCLLR 674
>PO21_NASVI (Q03278) Retrovirus-related Pol polyprotein from type I
retrotransposable element R2 [Contains: Reverse
transcriptase (EC 2.7.7.49); Endonuclease] (Fragment)
Length = 1025
Score = 42.0 bits (97), Expect = 0.015
Identities = 40/139 (28%), Positives = 65/139 (45%), Gaps = 9/139 (6%)
Query: 1368 KMKGKEKSVALKLDISKAYDRIDWAYLRDVMSKMGFSEK*I*WIMMWVGTVDYSVILNKE 1427
+MK K +A+ LD+ KA+D ++ + D + + + +IM + + K
Sbjct: 444 RMKIKGLYIAI-LDVKKAFDSVEHRSILDALRRKKLPLEMRNYIMWVYRNSKTRLEVVKT 502
Query: 1428 KIGPIIPGFGLRQGDPLSPYLFILCAEGLSAQIRNAESRGDLQGIRVCRIAPRVSHLLFA 1487
K I P G+RQGDPLSP LF + + ++ E+ G L G A ++ L+FA
Sbjct: 503 KGRWIRPARGVRQGDPLSPLLFNCVMDAVLRRL--PENTGFLMG------AEKIGALVFA 554
Query: 1488 DIDSV*GSIRSSYQSSRVR 1506
D + R Q+S R
Sbjct: 555 DDLVLLAETREGLQASLSR 573
>LIN1_HUMAN (P08547) LINE-1 reverse transcriptase homolog
Length = 1259
Score = 40.8 bits (94), Expect = 0.033
Identities = 32/111 (28%), Positives = 57/111 (50%), Gaps = 5/111 (4%)
Query: 1378 LKLDISKAYDRIDWAYLRDVMSKMGFSEK*I*WIMMWVGTVDYSVILNKEKIGPIIPGFG 1437
+ +D KA+D+I ++ ++K+G + I ++ILN +K+ G
Sbjct: 596 ISIDAEKAFDKIQQPFMLKPLNKLGIDGTYLKIIRAIYDKPTANIILNGQKLEAPPLKTG 655
Query: 1438 LRQGDPLSPYLFILCAEGLSAQIRNAESRGDLQGIRVCRIAPRVSHLLFAD 1488
RQG PLSP L + E L+ IR + +++GI++ + ++S LFAD
Sbjct: 656 TRQGCPLSPLLPNIVLEVLARAIRQEK---EIKGIQLGKEEVKLS--LFAD 701
>POLR_DROME (P16423) Retrovirus-related Pol polyprotein from type II
retrotransposable element R2DM [Contains: Protease (EC
3.4.23.-); Reverse transcriptase (EC 2.7.7.49);
Endonuclease]
Length = 1057
Score = 37.0 bits (84), Expect = 0.48
Identities = 23/77 (29%), Positives = 38/77 (48%)
Query: 1380 LDISKAYDRIDWAYLRDVMSKMGFSEK*I*WIMMWVGTVDYSVILNKEKIGPIIPGFGLR 1439
LD+SKA+D + A + D + G + + ++ S+ + +P G++
Sbjct: 481 LDVSKAFDSLSHASIYDTLRAYGAPKGFVDYVQNTYEGGGTSLNGDGWSSEEFVPARGVK 540
Query: 1440 QGDPLSPYLFILCAEGL 1456
QGDPLSP LF L + L
Sbjct: 541 QGDPLSPILFNLVMDRL 557
>PO22_POPJA (Q03274) Retrovirus-related Pol polyprotein from type I
retrotransposable element R2 [Contains: Reverse
transcriptase (EC 2.7.7.49); Endonuclease] (Fragment)
Length = 711
Score = 37.0 bits (84), Expect = 0.48
Identities = 32/136 (23%), Positives = 59/136 (42%), Gaps = 27/136 (19%)
Query: 1364 YMMTKMKGKEKSVALKLDISKAYDRIDWAYLRDVMSKMGFSEK*I*WIMMWVGTVDY--- 1420
Y+ ++ + ++ + LD+ KA+D + + + + ++G E GT +Y
Sbjct: 126 YISSRREQRKTYNVVSLDVRKAFDTVSHSSICRALQRLGIDE----------GTSNYITG 175
Query: 1421 --SVILNKEKIGP------IIPGFGLRQGDPLSPYLFILCAEGLSAQIRNAESRGDLQGI 1472
S ++GP I G++QGDPLSP+LF + L +++ G G
Sbjct: 176 SLSDSTTTIRVGPGSQTRKICIRRGVKQGDPLSPFLFNAVLDELLCSLQSTPGIGGTIG- 234
Query: 1473 RVCRIAPRVSHLLFAD 1488
++ L FAD
Sbjct: 235 -----EEKIPVLAFAD 245
>YO84_CAEEL (P34620) Hypothetical protein ZK1236.4 in chromosome III
Length = 364
Score = 35.4 bits (80), Expect = 1.4
Identities = 21/81 (25%), Positives = 40/81 (48%)
Query: 1380 LDISKAYDRIDWAYLRDVMSKMGFSEK*I*WIMMWVGTVDYSVILNKEKIGPIIPGFGLR 1439
LD SKA+D++ L D ++ + ++ I W+ +++ + V + P G+
Sbjct: 23 LDFSKAFDKVSHDILLDKLTSIKINKHLIRWLDVFLTNRSFKVKVGNTLSEPKKTVCGVP 82
Query: 1440 QGDPLSPYLFILCAEGLSAQI 1460
QG +SP LF + +SA +
Sbjct: 83 QGSVISPVLFGIFVNEISANL 103
>YKI3_YEAST (P36079) Hypothetical 23.7 kDa protein in MDH1-VMA5
intergenic region
Length = 204
Score = 35.0 bits (79), Expect = 1.8
Identities = 13/20 (65%), Positives = 17/20 (85%)
Query: 955 YIYIYIYIYIYIYIYIRIEY 974
YIYIY+Y+YIY++ YI I Y
Sbjct: 8 YIYIYMYLYIYVHTYIYIYY 27
Score = 34.7 bits (78), Expect = 2.4
Identities = 12/18 (66%), Positives = 17/18 (93%)
Query: 953 QYYIYIYIYIYIYIYIYI 970
++YIY YIYIY+Y+YIY+
Sbjct: 2 RWYIYRYIYIYMYLYIYV 19
Score = 33.1 bits (74), Expect = 6.9
Identities = 11/21 (52%), Positives = 17/21 (80%)
Query: 950 LFTQYYIYIYIYIYIYIYIYI 970
++ YIY+Y+YIY++ YIYI
Sbjct: 5 IYRYIYIYMYLYIYVHTYIYI 25
>PO21_SCICO (Q03279) Retrovirus-related Pol polyprotein from type I
retrotransposable element R2 [Contains: Reverse
transcriptase (EC 2.7.7.49); Endonuclease] (Fragment)
Length = 869
Score = 33.9 bits (76), Expect = 4.0
Identities = 35/117 (29%), Positives = 56/117 (46%), Gaps = 5/117 (4%)
Query: 1372 KEKSVALKLDISKAYDRIDWAYLRDVMSKMGFSEK*I*WIM-MWVGTVDYSVILNKEKIG 1430
KE +A+ LD+ KA++ + + L D +++ G + +I M+ + K ++
Sbjct: 289 KELHIAI-LDLVKAFNSVYHSALIDAITEAGCPPGVVDYIADMYNNVITEMQFEGKCELA 347
Query: 1431 PIIPGFGLRQGDPLSPYLFILCAEGLSAQIRNAESRGDLQGIRVCRIAPRVSHLLFA 1487
I+ G + QGDPLS LF L E + N E R D+ +RV A LL A
Sbjct: 348 SILAG--VYQGDPLSGPLFTLAYEKALRALNN-EGRFDIADVRVNASAYSDDGLLLA 401
>YIK0_YEAST (P40490) Hypothetical 13.5 kDa protein in MOB1-SGA1
intergenic region
Length = 117
Score = 33.1 bits (74), Expect = 6.9
Identities = 13/28 (46%), Positives = 20/28 (71%)
Query: 945 RGGN*LFTQYYIYIYIYIYIYIYIYIRI 972
R G + T Y +IYIYIY+Y+ +YI++
Sbjct: 20 RPGIHICTYIYAHIYIYIYVYVNVYIQL 47
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.357 0.159 0.586
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 173,512,806
Number of Sequences: 164201
Number of extensions: 6587745
Number of successful extensions: 25618
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 25576
Number of HSP's gapped (non-prelim): 29
length of query: 1735
length of database: 59,974,054
effective HSP length: 124
effective length of query: 1611
effective length of database: 39,613,130
effective search space: 63816752430
effective search space used: 63816752430
T: 11
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.7 bits)
S2: 73 (32.7 bits)
Medicago: description of AC134322.4