BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC125481.10 + phase: 0 /pseudo
(116 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PT2B_ARATH (P46032) Peptide transporter PTR2-B (Histidine transp... 104 3e-23
CHL1_ARATH (Q05085) Nitrate/chlorate transporter 93 9e-20
OPT1_DROME (P91679) Oligopeptide transporter 1 (YIN protein) 72 3e-13
PET2_HUMAN (Q16348) Oligopeptide transporter, kidney isoform (Pe... 62 2e-10
PET2_RAT (Q63424) Oligopeptide transporter, kidney isoform (Pept... 60 9e-10
PET1_RAT (P51574) Oligopeptide transporter, small intestine isof... 57 6e-09
PET1_HUMAN (P46059) Oligopeptide transporter, small intestine is... 57 7e-09
PET2_RABIT (P46029) Oligopeptide transporter, kidney isoform (Pe... 57 1e-08
PET1_RABIT (P36836) Oligopeptide transporter, small intestine is... 55 3e-08
PTR2_SCHPO (Q9P380) Probable peptide transporter Ptr2 (Peptide p... 54 6e-08
PT2A_ARATH (P46031) Peptide transporter PTR2-A 53 1e-07
OPT3_CAEEL (O01840) Putative oligopeptide transporter opt-3 52 3e-07
YBGH_ECOLI (P75742) Hypothetical transporter ybgH 51 4e-07
YJDL_ECOLI (P39276) Hypothetical transporter yjdL 51 5e-07
OPT2_CAEEL (Q21219) Oligopeptide transporter 2 (Di-/tri-peptide ... 48 3e-06
TPPB_SALTY (Q8ZPM6) Tripeptide permease tppB 47 8e-06
TPPB_SALTI (Q8Z6Q5) Tripeptide permease tppB 47 8e-06
TPPB_ECOLI (P77304) Tripeptide permease tppB 47 8e-06
TPPB_ECOL6 (Q8FH91) Tripeptide permease tppB 47 8e-06
TPPB_ECO57 (Q8X651) Tripeptide permease tppB 47 8e-06
>PT2B_ARATH (P46032) Peptide transporter PTR2-B (Histidine
transporting protein)
Length = 585
Score = 104 bits (260), Expect = 3e-23
Identities = 54/108 (50%), Positives = 65/108 (60%), Gaps = 2/108 (1%)
Query: 7 ETRLLLETVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPL 66
E +L E DG VDF G P L+ +G W+A FI+ E ER AYYGI NLI YLT L
Sbjct: 20 EVKLYAE--DGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKL 77
Query: 67 GQSTATAAENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
Q +AA NV W GT L PL+GA LAD++ GRY TI S +Y +
Sbjct: 78 HQGNVSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFI 125
>CHL1_ARATH (Q05085) Nitrate/chlorate transporter
Length = 590
Score = 93.2 bits (230), Expect = 9e-20
Identities = 46/92 (50%), Positives = 60/92 (65%)
Query: 20 DFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNI 79
DF+GRP RS +GGW +AA I+ +E ER GI NL+ YLTG + ATAA V
Sbjct: 17 DFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLGNATAANTVTN 76
Query: 80 WSGTASLLPLLGAFLADSFLGRYRTIIIASLV 111
+ GT+ +L LLG F+AD+FLGRY TI I + +
Sbjct: 77 FLGTSFMLCLLGGFIADTFLGRYLTIAIFAAI 108
>OPT1_DROME (P91679) Oligopeptide transporter 1 (YIN protein)
Length = 737
Score = 71.6 bits (174), Expect = 3e-13
Identities = 36/80 (45%), Positives = 52/80 (65%)
Query: 35 RAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFL 94
++ AFIIS E ERF YYG+ + L+ YLT LG + TA + ++ + PL+GA +
Sbjct: 28 KSVAFIISNEFCERFNYYGMRTILVLYLTNKLGYNEETATVLFHTFTMLVYIFPLIGALI 87
Query: 95 ADSFLGRYRTIIIASLVYIL 114
AD +LG+Y+TI+ SLVY L
Sbjct: 88 ADGWLGKYKTILYLSLVYSL 107
>PET2_HUMAN (Q16348) Oligopeptide transporter, kidney isoform
(Peptide transporter 2) (Kidney H(+)/peptide
cotransporter) (Solute carrier family 15, member 2)
Length = 729
Score = 62.0 bits (149), Expect = 2e-10
Identities = 33/77 (42%), Positives = 48/77 (61%)
Query: 38 AFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFLADS 97
AFI+ E ERF+YYG+ + LI Y L + T+ + +S P+LGA +ADS
Sbjct: 47 AFIVVNEFCERFSYYGMKAVLILYFLYFLHWNEDTSTSIYHAFSSLCYFTPILGAAIADS 106
Query: 98 FLGRYRTIIIASLVYIL 114
+LG+++TII SLVY+L
Sbjct: 107 WLGKFKTIIYLSLVYVL 123
>PET2_RAT (Q63424) Oligopeptide transporter, kidney isoform (Peptide
transporter 2) (Kidney H(+)/peptide cotransporter)
Length = 729
Score = 60.1 bits (144), Expect = 9e-10
Identities = 32/77 (41%), Positives = 47/77 (60%)
Query: 38 AFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFLADS 97
AFI+ E ERF+YYG+ + L Y L + T+ + +S P+LGA +ADS
Sbjct: 47 AFIVVNEFCERFSYYGMKAVLTLYFLYFLHWNEDTSTSVYHAFSSLCYFTPILGAAIADS 106
Query: 98 FLGRYRTIIIASLVYIL 114
+LG+++TII SLVY+L
Sbjct: 107 WLGKFKTIIYLSLVYVL 123
>PET1_RAT (P51574) Oligopeptide transporter, small intestine isoform
(Peptide transporter 1) (Intestinal H(+)/peptide
cotransporter) (Proton-coupled dipeptide cotransporter)
Length = 710
Score = 57.4 bits (137), Expect = 6e-09
Identities = 28/76 (36%), Positives = 45/76 (58%)
Query: 39 FIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFLADSF 98
FI+ E ERF+YYG+ + L+ Y LG + + + L P+LGA +ADS+
Sbjct: 18 FIVVNEFCERFSYYGMRALLVLYFRNFLGWDDDLSTAIYHTFVALCYLTPILGALIADSW 77
Query: 99 LGRYRTIIIASLVYIL 114
LG+++TI+ S+VY +
Sbjct: 78 LGKFKTIVSLSIVYTI 93
>PET1_HUMAN (P46059) Oligopeptide transporter, small intestine
isoform (Peptide transporter 1) (Intestinal H(+)/peptide
cotransporter) (Solute carrier family 15, member 1)
Length = 708
Score = 57.0 bits (136), Expect = 7e-09
Identities = 28/76 (36%), Positives = 45/76 (58%)
Query: 39 FIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFLADSF 98
FI+ E ERF+YYG+ + LI Y T + + + + L P+LGA +ADS+
Sbjct: 18 FIVVNEFCERFSYYGMRAILILYFTNFISWDDNLSTAIYHTFVALCYLTPILGALIADSW 77
Query: 99 LGRYRTIIIASLVYIL 114
LG+++TI+ S+VY +
Sbjct: 78 LGKFKTIVSLSIVYTI 93
>PET2_RABIT (P46029) Oligopeptide transporter, kidney isoform
(Peptide transporter 2) (Kidney H(+)/peptide
cotransporter)
Length = 729
Score = 56.6 bits (135), Expect = 1e-08
Identities = 31/77 (40%), Positives = 46/77 (59%)
Query: 38 AFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFLADS 97
AFI+ E ERF+YYG+ + L Y L + T+ + +S P+LGA +ADS
Sbjct: 47 AFIVVNEFCERFSYYGMKAVLTLYFLYFLHWNEDTSTSVYHAFSSLCYFTPILGAAIADS 106
Query: 98 FLGRYRTIIIASLVYIL 114
+LG+++TII SLV +L
Sbjct: 107 WLGKFKTIIYLSLVNVL 123
>PET1_RABIT (P36836) Oligopeptide transporter, small intestine
isoform (Peptide transporter 1) (Intestinal H(+)/peptide
cotransporter)
Length = 707
Score = 55.1 bits (131), Expect = 3e-08
Identities = 27/76 (35%), Positives = 45/76 (58%)
Query: 39 FIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFLADSF 98
FI+ E ERF+YYG+ + LI Y +G + + + L P+LGA +AD++
Sbjct: 18 FIVVNEFCERFSYYGMRALLILYFRNFIGWDDNLSTVIYHTFVALCYLTPILGALIADAW 77
Query: 99 LGRYRTIIIASLVYIL 114
LG+++TI+ S+VY +
Sbjct: 78 LGKFKTIVWLSIVYTI 93
>PTR2_SCHPO (Q9P380) Probable peptide transporter Ptr2 (Peptide
permease Ptr2)
Length = 618
Score = 53.9 bits (128), Expect = 6e-08
Identities = 35/92 (38%), Positives = 49/92 (53%), Gaps = 15/92 (16%)
Query: 34 WRAAAFIISVEVAERFAYYGINSNLINYLT-GP---------LGQSTATAAENV-NIWSG 82
W+A II VE+ ERFAYYG+ NY+ GP LG+S A N W
Sbjct: 83 WKAFIIII-VELCERFAYYGLTVPFQNYMQFGPKDATPGALNLGESGADGLSNFFTFW-- 139
Query: 83 TASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
+ P+ A +AD FLGRY TI+ ++++Y +
Sbjct: 140 -CYVTPVGAALIADQFLGRYNTIVCSAVIYFI 170
>PT2A_ARATH (P46031) Peptide transporter PTR2-A
Length = 610
Score = 52.8 bits (125), Expect = 1e-07
Identities = 34/92 (36%), Positives = 49/92 (52%), Gaps = 15/92 (16%)
Query: 34 WRAAAFIISVEVAERFAYYGINSNLINYLT-GP---------LGQSTATAAENV-NIWSG 82
W+A II VE+ ERFAYYG+ NY+ GP LG++ A N W
Sbjct: 83 WKAFIIII-VELCERFAYYGLTVPFQNYMQFGPKDATPGALNLGETGADGLSNFFTFW-- 139
Query: 83 TASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
+ P+ A +AD FLGRY TI+ ++++Y +
Sbjct: 140 -CYVTPVGAALIADQFLGRYNTIVCSAVIYFI 170
>OPT3_CAEEL (O01840) Putative oligopeptide transporter opt-3
Length = 701
Score = 51.6 bits (122), Expect = 3e-07
Identities = 26/79 (32%), Positives = 46/79 (57%)
Query: 36 AAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFLA 95
A F++ E ERF++YG+ + L YL S + A ++++ A L PL+G+ +A
Sbjct: 16 AVFFMLGNEFCERFSFYGMKTILFIYLITEHEFSPSKATFIYHLFTCIAYLTPLIGSIMA 75
Query: 96 DSFLGRYRTIIIASLVYIL 114
DS GR++ I+ S +Y++
Sbjct: 76 DSVFGRFKVILYGSSIYVV 94
>YBGH_ECOLI (P75742) Hypothetical transporter ybgH
Length = 493
Score = 51.2 bits (121), Expect = 4e-07
Identities = 24/77 (31%), Positives = 45/77 (58%)
Query: 35 RAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFL 94
RA +++++++ E F++YG+ + LI YLT L + A E + + + P+LG FL
Sbjct: 9 RAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYNDTHAYELFSAYCSLVYVTPILGGFL 68
Query: 95 ADSFLGRYRTIIIASLV 111
AD LG +++ +L+
Sbjct: 69 ADKVLGNRMAVMLGALL 85
>YJDL_ECOLI (P39276) Hypothetical transporter yjdL
Length = 485
Score = 50.8 bits (120), Expect = 5e-07
Identities = 26/80 (32%), Positives = 45/80 (55%)
Query: 35 RAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFL 94
RA +I+++++ E F++YG+ + LI YLT LG A + ++ + P+LG +L
Sbjct: 8 RAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDNHAISLFSAYASLVYVTPILGGWL 67
Query: 95 ADSFLGRYRTIIIASLVYIL 114
AD LG +I +L+ L
Sbjct: 68 ADRLLGNRTAVIAGALLMTL 87
>OPT2_CAEEL (Q21219) Oligopeptide transporter 2 (Di-/tri-peptide
transporter CPTB)
Length = 835
Score = 48.1 bits (113), Expect = 3e-06
Identities = 25/75 (33%), Positives = 43/75 (57%)
Query: 40 IISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFLADSFL 99
I+S E ERF+YYG+ + L YL L + + + N ++ PLLG+ +AD ++
Sbjct: 51 IVSNEFCERFSYYGMRTVLTFYLLNVLKFTDSQSTIFFNGFTVLCYTTPLLGSIVADGYI 110
Query: 100 GRYRTIIIASLVYIL 114
G++ TI S++Y +
Sbjct: 111 GKFWTIFSVSILYAI 125
>TPPB_SALTY (Q8ZPM6) Tripeptide permease tppB
Length = 501
Score = 47.0 bits (110), Expect = 8e-06
Identities = 26/77 (33%), Positives = 40/77 (51%)
Query: 35 RAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFL 94
+A I S+E+ ERF YYG+ + YL LG S A + + +S L +G +L
Sbjct: 21 KAFYLIFSIELWERFGYYGLQGIMAVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWL 80
Query: 95 ADSFLGRYRTIIIASLV 111
D LG R I++ ++V
Sbjct: 81 GDKILGTKRVIMLGAVV 97
>TPPB_SALTI (Q8Z6Q5) Tripeptide permease tppB
Length = 501
Score = 47.0 bits (110), Expect = 8e-06
Identities = 26/77 (33%), Positives = 40/77 (51%)
Query: 35 RAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFL 94
+A I S+E+ ERF YYG+ + YL LG S A + + +S L +G +L
Sbjct: 21 KAFYLIFSIELWERFGYYGLQGIMAVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWL 80
Query: 95 ADSFLGRYRTIIIASLV 111
D LG R I++ ++V
Sbjct: 81 GDKILGTKRVIMLGAVV 97
>TPPB_ECOLI (P77304) Tripeptide permease tppB
Length = 500
Score = 47.0 bits (110), Expect = 8e-06
Identities = 26/77 (33%), Positives = 40/77 (51%)
Query: 35 RAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFL 94
+A I S+E+ ERF YYG+ + YL LG S A + + +S L +G +L
Sbjct: 21 KAFYLIFSIELWERFGYYGLQGIMAVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWL 80
Query: 95 ADSFLGRYRTIIIASLV 111
D LG R I++ ++V
Sbjct: 81 GDKVLGTKRVIMLGAIV 97
>TPPB_ECOL6 (Q8FH91) Tripeptide permease tppB
Length = 500
Score = 47.0 bits (110), Expect = 8e-06
Identities = 26/77 (33%), Positives = 40/77 (51%)
Query: 35 RAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFL 94
+A I S+E+ ERF YYG+ + YL LG S A + + +S L +G +L
Sbjct: 21 KAFYLIFSIELWERFGYYGLQGIMAVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWL 80
Query: 95 ADSFLGRYRTIIIASLV 111
D LG R I++ ++V
Sbjct: 81 GDKVLGTKRVIMLGAIV 97
>TPPB_ECO57 (Q8X651) Tripeptide permease tppB
Length = 500
Score = 47.0 bits (110), Expect = 8e-06
Identities = 26/77 (33%), Positives = 40/77 (51%)
Query: 35 RAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFL 94
+A I S+E+ ERF YYG+ + YL LG S A + + +S L +G +L
Sbjct: 21 KAFYLIFSIELWERFGYYGLQGIMAVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWL 80
Query: 95 ADSFLGRYRTIIIASLV 111
D LG R I++ ++V
Sbjct: 81 GDKVLGTKRVIMLGAIV 97
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.323 0.139 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,127,685
Number of Sequences: 164201
Number of extensions: 414010
Number of successful extensions: 1058
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 29
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 1022
Number of HSP's gapped (non-prelim): 37
length of query: 116
length of database: 59,974,054
effective HSP length: 92
effective length of query: 24
effective length of database: 44,867,562
effective search space: 1076821488
effective search space used: 1076821488
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC125481.10