BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC123572.11 + phase: 0
(475 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
RGSI_MOUSE (Q99PG4) Regulator of G-protein signaling 18 (RGS18) 41 0.008
RGSI_HUMAN (Q9NS28) Regulator of G-protein signaling 18 (RGS18) 38 0.065
RGS5_HUMAN (O15539) Regulator of G-protein signaling 5 (RGS5) 36 0.19
LNT_THEMA (Q9WZ43) Apolipoprotein N-acyltransferase (EC 2.3.1.-)... 36 0.19
RGSG_MOUSE (P97428) Regulator of G-protein signaling 16 (RGS16) ... 36 0.25
RGS5_MOUSE (O08850) Regulator of G-protein signaling 5 (RGS5) 36 0.25
YCAK_CAEEL (Q18563) Hypothetical protein C41G11.3 in chromosome X 35 0.42
RGSG_RAT (P56700) Regulator of G-protein signaling 16 (RGS16) (R... 34 0.72
RGSG_HUMAN (O15492) Regulator of G-protein signaling 16 (RGS16) ... 34 0.72
RGS5_RAT (P49800) Regulator of G-protein signaling 5 (RGS5) 34 0.72
T2R3_PANTR (Q646A7) Taste receptor type 2 member 3 (T2R3) 34 0.94
T2R3_PANPA (Q646D2) Taste receptor type 2 member 3 (T2R3) 34 0.94
T2R3_HUMAN (Q9NYW6) Taste receptor type 2 member 3 (T2R3) 34 0.94
FLBA_EMENI (P38093) Developmental regulator flbA 34 0.94
TRMU_BUCAP (Q8K9Q8) Probable tRNA (5-methylaminomethyl-2-thiouri... 33 1.6
IM23_SCHHA (Q26499) 23 kDa integral membrane protein (Sh23) 33 1.6
RGSC_RAT (O08774) Regulator of G-protein signaling 12 (RGS12) 33 2.1
RGSC_HUMAN (O14924) Regulator of G-protein signaling 12 (RGS12) 33 2.1
RGSG_BOVIN (O46471) Regulator of G-protein signaling 16 (RGS16) ... 32 2.7
IM23_SCHMA (P19331) 23 kDa integral membrane protein (SM23) 32 2.7
>RGSI_MOUSE (Q99PG4) Regulator of G-protein signaling 18 (RGS18)
Length = 235
Score = 40.8 bits (94), Expect = 0.008
Identities = 33/133 (24%), Positives = 57/133 (42%), Gaps = 2/133 (1%)
Query: 293 TQSEPISRVDPNEPLDKLLLNKKFRQSFMGFADSCLAGESVHFFDEVHELSKISEHDCVR 352
T+ P V E DKLL ++ +F F + + E++ F+ + K E +
Sbjct: 73 TRVSPEEAVKWAESFDKLLSHRDGVDAFTRFLKTEFSEENIEFWVACEDFKKCKEPQQI- 131
Query: 353 RIYMARHIIEKYMVAGAAMEINISHRSKQEILSTSDLARADLFHNALNEIVHLMKTNLAK 412
I A+ I EK++ A E+NI +K+ I + F A + + LM+ + K
Sbjct: 132 -ILKAKAIYEKFIQNDAPKEVNIDFHTKEVIAKSIAQPTLHSFDTAQSRVYQLMEHDSYK 190
Query: 413 DYWSSMFFLKFQE 425
+ S +L E
Sbjct: 191 RFLKSETYLHLIE 203
>RGSI_HUMAN (Q9NS28) Regulator of G-protein signaling 18 (RGS18)
Length = 235
Score = 37.7 bits (86), Expect = 0.065
Identities = 29/133 (21%), Positives = 56/133 (41%), Gaps = 2/133 (1%)
Query: 293 TQSEPISRVDPNEPLDKLLLNKKFRQSFMGFADSCLAGESVHFFDEVHELSKISEHDCVR 352
T+ P V E DKLL ++ ++F F + + E++ F+ + K +
Sbjct: 73 TRVSPEEAVKWGESFDKLLSHRDGLEAFTRFLKTEFSEENIEFWIACEDFKKSKGPQQIH 132
Query: 353 RIYMARHIIEKYMVAGAAMEINISHRSKQEILSTSDLARADLFHNALNEIVHLMKTNLAK 412
A+ I EK++ A E+N+ +K+ I ++ F A + + LM+ +
Sbjct: 133 --LKAKAIYEKFIQTDAPKEVNLDFHTKEVITNSITQPTLHSFDAAQSRVYQLMEQDSYT 190
Query: 413 DYWSSMFFLKFQE 425
+ S +L E
Sbjct: 191 RFLKSDIYLDLME 203
>RGS5_HUMAN (O15539) Regulator of G-protein signaling 5 (RGS5)
Length = 181
Score = 36.2 bits (82), Expect = 0.19
Identities = 30/116 (25%), Positives = 48/116 (40%), Gaps = 2/116 (1%)
Query: 305 EPLDKLLLNKKFRQSFMGFADSCLAGESVHFFDEVHELSKISEHDCVRRIYMARHIIEKY 364
+ LDKLL N SF F S + E++ F+ + KI + A+ I E++
Sbjct: 63 DSLDKLLQNNYGLASFKSFLKSEFSEENLEFWIACEDYKKIKSP--AKMAEKAKQIYEEF 120
Query: 365 MVAGAAMEINISHRSKQEILSTSDLARADLFHNALNEIVHLMKTNLAKDYWSSMFF 420
+ A E+NI H +K + F A I LM+ + + S F+
Sbjct: 121 IQTEAPKEVNIDHFTKDITMKNLVEPSLSSFDMAQKRIHALMEKDSLPRFVRSEFY 176
>LNT_THEMA (Q9WZ43) Apolipoprotein N-acyltransferase (EC 2.3.1.-)
(ALP N-acyltransferase)
Length = 503
Score = 36.2 bits (82), Expect = 0.19
Identities = 37/140 (26%), Positives = 66/140 (46%), Gaps = 21/140 (15%)
Query: 21 VSILSFILLLIWSIFPFIVHKVPRTKGSGF--WIPVIQVVASFNLLLSIMTISSNLKRAI 78
+ I+ F+L+ I PF+ G GF + +V L S+ TI L R +
Sbjct: 100 LGIVVFVLMGIIEAVPFL--------GFGFLSYFAPQSIVLKTLYLASVYTIFEYL-RGV 150
Query: 79 GGSHVTCGQVS--FHNHTLCLFPVWGEGPLGFGLLLSSRITQAFQLYFIFVKRRLPLIRS 136
G T G++S ++HT + V G LG L+ S L++ F++RR +
Sbjct: 151 GELGFTGGRISEALYSHTGLIQIVSITGTLGLVFLIVSLNV----LFYEFLRRR----KG 202
Query: 137 FLLIPLILLPWIIGAAVIHI 156
L+ P+I +++ ++V+H+
Sbjct: 203 LLIFPVIFFVYLLNSSVVHL 222
>RGSG_MOUSE (P97428) Regulator of G-protein signaling 16 (RGS16)
(Retinally abundant regulator of G-protein signaling)
(RGS-R) (A28-RGS14P)
Length = 201
Score = 35.8 bits (81), Expect = 0.25
Identities = 26/103 (25%), Positives = 45/103 (43%), Gaps = 2/103 (1%)
Query: 305 EPLDKLLLNKKFRQSFMGFADSCLAGESVHFFDEVHELSKISEHDCVRRIYMARHIIEKY 364
E D LL +K +F F + + E++ F+ E KI + A HI ++Y
Sbjct: 63 ESFDLLLNSKNGVAAFHAFLKTEFSEENLEFWLACEEFKKI--RSATKLASRAHHIFDEY 120
Query: 365 MVAGAAMEINISHRSKQEILSTSDLARADLFHNALNEIVHLMK 407
+ + A E+NI H +++ + A F A + LM+
Sbjct: 121 IRSEAPKEVNIDHETRELTKTNLQAATTSCFDVAQGKTRTLME 163
>RGS5_MOUSE (O08850) Regulator of G-protein signaling 5 (RGS5)
Length = 181
Score = 35.8 bits (81), Expect = 0.25
Identities = 30/116 (25%), Positives = 48/116 (40%), Gaps = 2/116 (1%)
Query: 305 EPLDKLLLNKKFRQSFMGFADSCLAGESVHFFDEVHELSKISEHDCVRRIYMARHIIEKY 364
+ LDKLL N SF F S + E++ F+ KI ++ A+ I E++
Sbjct: 63 QSLDKLLQNSYGFASFKSFLKSEFSEENLEFWVACENYKKIKSP--IKMAEKAKQIYEEF 120
Query: 365 MVAGAAMEINISHRSKQEILSTSDLARADLFHNALNEIVHLMKTNLAKDYWSSMFF 420
+ A E+NI H +K + F A I LM+ + + S F+
Sbjct: 121 IQTEAPKEVNIDHFTKDITMKNLVEPSPRSFDLAQKRIYALMEKDSLPRFVRSEFY 176
>YCAK_CAEEL (Q18563) Hypothetical protein C41G11.3 in chromosome X
Length = 737
Score = 35.0 bits (79), Expect = 0.42
Identities = 23/101 (22%), Positives = 45/101 (43%), Gaps = 4/101 (3%)
Query: 309 KLLLNKKFRQSFMGFADSCLAGESVHFFDEVHELSKISEHDCVRRIYMARHIIEKYMVAG 368
K++ + R F F + E+++F+ V + +I D R R I ++Y
Sbjct: 21 KVIRDPVLRAPFQEFLEQQFCAENLNFYLAVEQFKEIQ--DFQERSSFGRRIFDRYFAMN 78
Query: 369 AAMEINISHRSKQEILSTSDLA--RADLFHNALNEIVHLMK 407
+ +NI + + + I T + D + A +I+HL+K
Sbjct: 79 STEPVNIDNSTSKRIRETVESGGFPLDTYDVAQYQILHLLK 119
>RGSG_RAT (P56700) Regulator of G-protein signaling 16 (RGS16)
(Retinally abundant regulator of G-protein signaling)
(RGS-R)
Length = 199
Score = 34.3 bits (77), Expect = 0.72
Identities = 25/103 (24%), Positives = 44/103 (42%), Gaps = 2/103 (1%)
Query: 305 EPLDKLLLNKKFRQSFMGFADSCLAGESVHFFDEVHELSKISEHDCVRRIYMARHIIEKY 364
E LL +K +F F + + E++ F+ E KI + A HI ++Y
Sbjct: 63 ESFQSLLNSKNGVAAFHAFLKTEFSEENLEFWLACEEFKKI--RSATKLASRAHHIFDEY 120
Query: 365 MVAGAAMEINISHRSKQEILSTSDLARADLFHNALNEIVHLMK 407
+ + A E+NI H +++ + A F A + LM+
Sbjct: 121 IRSEAPKEVNIDHETRELTKTNLQAATTSCFDVAQGKTRTLME 163
>RGSG_HUMAN (O15492) Regulator of G-protein signaling 16 (RGS16)
(Retinally abundant regulator of G-protein signaling)
(RGS-R) (A28-RGS14P)
Length = 202
Score = 34.3 bits (77), Expect = 0.72
Identities = 26/103 (25%), Positives = 44/103 (42%), Gaps = 2/103 (1%)
Query: 305 EPLDKLLLNKKFRQSFMGFADSCLAGESVHFFDEVHELSKISEHDCVRRIYMARHIIEKY 364
E D LL +K +F F + + E++ F+ E KI + A I E++
Sbjct: 64 ESFDLLLSSKNGVAAFHAFLKTEFSEENLEFWLACEEFKKI--RSATKLASRAHQIFEEF 121
Query: 365 MVAGAAMEINISHRSKQEILSTSDLARADLFHNALNEIVHLMK 407
+ + A E+NI H +++ A A F A + LM+
Sbjct: 122 ICSEAPKEVNIDHETRELTRMNLQTATATCFDAAQGKTRTLME 164
>RGS5_RAT (P49800) Regulator of G-protein signaling 5 (RGS5)
Length = 181
Score = 34.3 bits (77), Expect = 0.72
Identities = 29/116 (25%), Positives = 48/116 (41%), Gaps = 2/116 (1%)
Query: 305 EPLDKLLLNKKFRQSFMGFADSCLAGESVHFFDEVHELSKISEHDCVRRIYMARHIIEKY 364
+ LDKLL + SF F S + E++ F+ KI ++ A+ I E++
Sbjct: 63 QSLDKLLQSNYGFASFKSFLKSEFSEENLEFWVACENYKKIKSP--IKMAEKAKQIYEEF 120
Query: 365 MVAGAAMEINISHRSKQEILSTSDLARADLFHNALNEIVHLMKTNLAKDYWSSMFF 420
+ A E+NI H +K + F A I LM+ + + S F+
Sbjct: 121 IQTEAPKEVNIDHFTKDITMKNLVEPSPHSFDLAQKRIYALMEKDSLPRFVRSEFY 176
>T2R3_PANTR (Q646A7) Taste receptor type 2 member 3 (T2R3)
Length = 315
Score = 33.9 bits (76), Expect = 0.94
Identities = 30/122 (24%), Positives = 55/122 (44%), Gaps = 16/122 (13%)
Query: 14 TDYVAVTVSILSFILLLIWSIFPFIVHKVPRTKGSGFWIPVIQVVASFNLLLSIMTISSN 73
+D++ T+++L ILL I F++ P T SG + +I V +F LSI
Sbjct: 43 SDFIITTLALLRIILLCIILTDSFLIEFSPNTHDSGIIMQIIDVSWTFTNHLSIW----- 97
Query: 74 LKRAIGGSHVTCGQVSFHNHTLCLFPVWGEGPLGFGLLL---------SSRITQAFQLYF 124
L +G + C +++ +H L+ W + +LL ++ + F+LY
Sbjct: 98 LATCLGVLY--CLKIASFSHPTFLWLKWRVSRVMVWMLLGALLLSCGSTASLINEFKLYS 155
Query: 125 IF 126
+F
Sbjct: 156 VF 157
>T2R3_PANPA (Q646D2) Taste receptor type 2 member 3 (T2R3)
Length = 315
Score = 33.9 bits (76), Expect = 0.94
Identities = 30/122 (24%), Positives = 55/122 (44%), Gaps = 16/122 (13%)
Query: 14 TDYVAVTVSILSFILLLIWSIFPFIVHKVPRTKGSGFWIPVIQVVASFNLLLSIMTISSN 73
+D++ T+++L ILL I F++ P T SG + +I V +F LSI
Sbjct: 43 SDFIITTLALLRIILLCIILTDSFLIEFSPNTHDSGIIMQIIDVSWTFTNHLSIW----- 97
Query: 74 LKRAIGGSHVTCGQVSFHNHTLCLFPVWGEGPLGFGLLL---------SSRITQAFQLYF 124
L +G + C +++ +H L+ W + +LL ++ + F+LY
Sbjct: 98 LATCLGVLY--CLKIASFSHPTFLWLKWRVSRVMVWMLLGALLLSCGSTASLINEFKLYS 155
Query: 125 IF 126
+F
Sbjct: 156 VF 157
>T2R3_HUMAN (Q9NYW6) Taste receptor type 2 member 3 (T2R3)
Length = 315
Score = 33.9 bits (76), Expect = 0.94
Identities = 30/122 (24%), Positives = 55/122 (44%), Gaps = 16/122 (13%)
Query: 14 TDYVAVTVSILSFILLLIWSIFPFIVHKVPRTKGSGFWIPVIQVVASFNLLLSIMTISSN 73
+D++ T+++L ILL I F++ P T SG + +I V +F LSI
Sbjct: 43 SDFIITTLALLRIILLCIILTDSFLIEFSPNTHDSGIIMQIIDVSWTFTNHLSIW----- 97
Query: 74 LKRAIGGSHVTCGQVSFHNHTLCLFPVWGEGPLGFGLLL---------SSRITQAFQLYF 124
L +G + C +++ +H L+ W + +LL ++ + F+LY
Sbjct: 98 LATCLGVLY--CLKIASFSHPTFLWLKWRVSRVMVWMLLGALLLSCGSTASLINEFKLYS 155
Query: 125 IF 126
+F
Sbjct: 156 VF 157
>FLBA_EMENI (P38093) Developmental regulator flbA
Length = 719
Score = 33.9 bits (76), Expect = 0.94
Identities = 31/126 (24%), Positives = 52/126 (40%), Gaps = 13/126 (10%)
Query: 295 SEPISRVDPNEPLDKLLLNKKFRQSFMGFADSCLAGESVHFFDEV-------HELSKISE 347
S I R N L+ +L + R F F L E++ F+ +V H+ K+
Sbjct: 533 SRGIPRDSNNARLNHILQDPALRLLFREFLRFSLCEENLSFYIDVSEFTTQYHKFDKVGH 592
Query: 348 H---DCVRRIYMARH-IIEKYMVAGAAMEINISHRSKQEILS--TSDLARADLFHNALNE 401
D VR A + + ++ G+ E+NI H + + S T + D +L E
Sbjct: 593 FKKPDAVRETLAAAYGLYNAFLAPGSPCELNIDHALRNSLASRMTKAVGDDDSMLKSLQE 652
Query: 402 IVHLMK 407
+V L +
Sbjct: 653 VVQLFE 658
>TRMU_BUCAP (Q8K9Q8) Probable tRNA
(5-methylaminomethyl-2-thiouridylate)-methyltransferase
(EC 2.1.1.61)
Length = 367
Score = 33.1 bits (74), Expect = 1.6
Identities = 19/58 (32%), Positives = 28/58 (47%), Gaps = 2/58 (3%)
Query: 362 EKYMVAGAAMEINISHRSKQEILSTSDLARADLFHNALNEIVHLMKTNLAKDYWSSMF 419
E Y V G M+ K+ + DL+ A+ LN +HL K N +K+YW +F
Sbjct: 28 ENYQVEGLFMKNWEEDDEKEYCNAAKDLSDAEEVCKKLN--IHLHKVNFSKEYWEKVF 83
>IM23_SCHHA (Q26499) 23 kDa integral membrane protein (Sh23)
Length = 218
Score = 33.1 bits (74), Expect = 1.6
Identities = 27/113 (23%), Positives = 55/113 (47%), Gaps = 13/113 (11%)
Query: 163 RCHMSVQWTIPVVCLHALYVATLVGVTAAVHHIEFRF----DELRDLWRG--ILVSSVSV 216
RC S + + ++CL L L+G A ++E +F D L +W+ I + V V
Sbjct: 9 RCLKSCVFVLNIICL--LCSLVLIGAGA---YVEVKFSQYGDNLHKVWQAAPIAIIVVGV 63
Query: 217 AVWVTAYI--LNEIHDNISWLQVVSRFLLLVLASILVLAFFSISSSQPLLSQI 267
+ + +++ I +N+ L + + FL+++L + L A ++ + S+I
Sbjct: 64 IILIVSFLGCCGAIKENVCMLYMYAFFLIILLIAELAAAIVAVVYKDRIDSEI 116
>RGSC_RAT (O08774) Regulator of G-protein signaling 12 (RGS12)
Length = 1387
Score = 32.7 bits (73), Expect = 2.1
Identities = 28/122 (22%), Positives = 53/122 (42%), Gaps = 9/122 (7%)
Query: 308 DKLLLNKKFRQSFMGFADSCLAGESVHFFDEVHELSKISEHDCVRRIYMARHIIEKYMVA 367
++LL + + F F + E++ F+ S + HD Y AR I K++ +
Sbjct: 717 ERLLQDPVGVRYFSDFLRKEFSEENILFWQACECFSHVPAHDKKELSYRAREIFSKFLCS 776
Query: 368 GAAMEINISHRSK--QEILSTSDLARADLFHNALNEIVHLMKTNLAKDYWSSMFFLKFQE 425
A +NI +++ +IL+ D+F +I +LMK + + S + +
Sbjct: 777 KATTPVNIDSQAQLADDILNA---PHPDMFKEQQLQIFNLMKFDSYTRFLKSQLY----Q 829
Query: 426 EC 427
EC
Sbjct: 830 EC 831
>RGSC_HUMAN (O14924) Regulator of G-protein signaling 12 (RGS12)
Length = 1447
Score = 32.7 bits (73), Expect = 2.1
Identities = 25/103 (24%), Positives = 46/103 (44%), Gaps = 7/103 (6%)
Query: 308 DKLLLNKKFRQSFMGFADSCLAGESVHFFDEVHELSKISEHDCVRRIYMARHIIEKYMVA 367
++LL + + F F + E++ F+ + + HD Y AR I K++ +
Sbjct: 717 ERLLQDPVGVRYFSDFLRKEFSEENILFWQACEYFNHVPAHDKKELSYRAREIFSKFLCS 776
Query: 368 GAAMEINISHRSKQEILSTSDLARA---DLFHNALNEIVHLMK 407
A +NI +++ D+ RA D+F +I +LMK
Sbjct: 777 KATTPVNIDSQAQ----LADDVLRAPHPDMFKEQQLQIFNLMK 815
>RGSG_BOVIN (O46471) Regulator of G-protein signaling 16 (RGS16)
(Retinally abundant regulator of G-protein signaling)
(RGS-R)
Length = 202
Score = 32.3 bits (72), Expect = 2.7
Identities = 25/103 (24%), Positives = 46/103 (44%), Gaps = 2/103 (1%)
Query: 305 EPLDKLLLNKKFRQSFMGFADSCLAGESVHFFDEVHELSKISEHDCVRRIYMARHIIEKY 364
E D LL +K +F F + + E++ F+ E K+ + A I E++
Sbjct: 64 ESFDLLLSSKNGVAAFHAFLKTEFSEENLEFWLACEEFKKL--RSATKLGSRAHRIFEEF 121
Query: 365 MVAGAAMEINISHRSKQEILSTSDLARADLFHNALNEIVHLMK 407
+ + A E+NI H +++ + A A F A ++ LM+
Sbjct: 122 ICSEAPKEVNIDHETRELTRTNLQAATAVCFDAAQWKVRALME 164
>IM23_SCHMA (P19331) 23 kDa integral membrane protein (SM23)
Length = 218
Score = 32.3 bits (72), Expect = 2.7
Identities = 27/113 (23%), Positives = 55/113 (47%), Gaps = 13/113 (11%)
Query: 163 RCHMSVQWTIPVVCLHALYVATLVGVTAAVHHIEFRF----DELRDLWRG--ILVSSVSV 216
RC S + + ++CL L L+G A ++E +F D L +W+ I + V V
Sbjct: 9 RCLKSCVFVLNIICL--LCSLVLIGAGA---YVEVKFSQYGDNLHKVWQAAPIAIIVVGV 63
Query: 217 AVWVTAYI--LNEIHDNISWLQVVSRFLLLVLASILVLAFFSISSSQPLLSQI 267
+ + +++ I +N+ L + + FL+++L + L A ++ + S+I
Sbjct: 64 IILIVSFLGCCGAIKENVCMLYMYAFFLVVLLIAELAAAIVAVVYKDRIDSEI 116
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.327 0.140 0.432
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,409,308
Number of Sequences: 164201
Number of extensions: 2100058
Number of successful extensions: 6011
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 24
Number of HSP's that attempted gapping in prelim test: 6007
Number of HSP's gapped (non-prelim): 28
length of query: 475
length of database: 59,974,054
effective HSP length: 114
effective length of query: 361
effective length of database: 41,255,140
effective search space: 14893105540
effective search space used: 14893105540
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 68 (30.8 bits)
Medicago: description of AC123572.11