BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC130802.4 + phase: 0
(634 letters)
Database: nr
2,540,612 sequences; 863,360,394 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T52331 pectinesterase (EC 3.1.1.11) [imported] - Salix gilg... 598 e-169
emb|CAB80040.1| pectinesterase-like protein [Arabidopsis thalian... 494 e-138
dbj|BAD33558.1| putative pectinesterase [Oryza sativa (japonica ... 484 e-135
gb|AAP40488.1| putative pectin methylesterase [Arabidopsis thali... 466 e-130
emb|CAD40902.1| OSJNBa0036B21.20 [Oryza sativa (japonica cultiva... 413 e-114
ref|NP_198139.1| pectinesterase family protein [Arabidopsis thal... 399 e-109
ref|XP_470886.1| putative pectin methylesterase [Oryza sativa (j... 395 e-108
emb|CAA73733.1| pectin methylesterase-like protein [Zea mays] gi... 392 e-107
ref|NP_850077.1| pectinesterase family protein [Arabidopsis thal... 391 e-107
gb|AAC14494.1| putative pectinesterase [Arabidopsis thaliana] gi... 391 e-107
gb|AAM65978.1| pectin methylesterase [Arabidopsis thaliana] gi|1... 389 e-106
ref|XP_482698.1| putative pectinesterase [Oryza sativa (japonica... 385 e-105
gb|AAF26136.1| putative pectinesterase [Arabidopsis thaliana] gi... 348 2e-94
dbj|BAC42986.1| putative pectinesterase [Arabidopsis thaliana] g... 345 3e-93
gb|AAB57670.1| pectinesterase [Citrus sinensis] 337 5e-91
sp|O04886|PME1_CITSI Pectinesterase-1 precursor (Pectin methyles... 337 7e-91
sp|P83948|PME3_CITSI Pectinesterase-3 precursor (Pectin methyles... 335 3e-90
dbj|BAD45460.1| putative pectinesterase [Oryza sativa (japonica ... 330 7e-89
ref|XP_474065.1| OSJNBb0079B02.7 [Oryza sativa (japonica cultiva... 327 6e-88
emb|CAC09467.1| putative pectin methylesterase [Oryza sativa (in... 327 6e-88
>pir||T52331 pectinesterase (EC 3.1.1.11) [imported] - Salix gilgiana
gi|6714532|dbj|BAA89480.1| pectin methylesterase [Salix
gilgiana]
Length = 596
Score = 598 bits (1543), Expect = e-169
Identities = 317/641 (49%), Positives = 413/641 (63%), Gaps = 54/641 (8%)
Query: 1 MAFEDFDLVSERRKANAKQHLKKKILIGVTSVVLIACVIAAVTFVIVKR---SGPDHNNN 57
M F+DFD +SERR+ +Q L+KKI+I S + ++ A F +V S P +N
Sbjct: 1 MVFQDFDQLSERRRLERQQKLRKKIIIASVSSIAFFVIVGAGVFSLVSNHDISSPGNNGG 60
Query: 58 DKKPVQNAPPEPERVDKYSRLVTMLCSHSEYKEKCVTTL-KEALKKDPKLKEPKGLLMVF 116
+ + + +R++ +C+ + Y++ C TL K L KDP +PK LL +
Sbjct: 61 SPSAATRPAEKAKPISHVARVIKTVCNATTYQDTCQNTLEKGVLGKDPSSVQPKDLLKIA 120
Query: 117 MLVAKNEINNAFNKTANLKFASKEEKGAYEDCKQLFEDAKEEMGFSITEVGQLDISKLAS 176
+ A EI+ K ++ KF EK A++DC +L EDAKEE+ S+ +G DI KLAS
Sbjct: 121 IKAADEEIDKVIKKASSFKFDKPREKAAFDDCLELIEDAKEELKNSVDCIGN-DIGKLAS 179
Query: 177 KEAELNNWLSAVISYQDTCSDGFPEGELKKKMEMIFAESRQLLSNSLAVVSQVSQIVN-- 234
+L+NWLSAV+SYQ TC DGFPEG+LK ME F +R+L SNSLA+VS + +
Sbjct: 180 NAPDLSNWLSAVMSYQQTCIDGFPEGKLKSDMEKTFKATRELTSNSLAMVSSLVSFLKNF 239
Query: 235 AFQGGLSGFKLPWGKSDAHAPAPDADTDAVADDDEDLADAPDGAPDADQPIFEAPIGAPG 294
+F G L+ L ++ +P D D V P D+ I +
Sbjct: 240 SFSGTLNRRLLAEEQN-----SPSLDKDGV----------PGWMSHEDRRILK------- 277
Query: 295 AAPIGAPRVDAPPSWAAPAVDLPGSTEKPTPNVTVAKDGSGDFKTISEALAAIPQTYKGR 354
GA + +KP PNV+VAKDGSGDFKTISEALAA+P Y+GR
Sbjct: 278 ----GADK------------------DKPKPNVSVAKDGSGDFKTISEALAAMPAKYEGR 315
Query: 355 YVVYVKEGVYDETVTVTKKMVNLTMYGDGGLKSIITGNKNFVDGVRTFQTASFVVLGDGF 414
YV++VK+GVYDETVTVTKKM N+TMYGDG K+I+TGNKNF DGV+TF+TA+F VLGDGF
Sbjct: 316 YVIFVKQGVYDETVTVTKKMANITMYGDGSQKTIVTGNKNFADGVQTFRTATFAVLGDGF 375
Query: 415 VGRDMGFRNTAGAIKEQAVAARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTI 474
+ + MGFRNTAG K QAVA RVQAD +IF+NC FEGYQDTLYAQTHRQFYR CVI+GT+
Sbjct: 376 LCKFMGFRNTAGPEKHQAVAIRVQADRAIFLNCRFEGYQDTLYAQTHRQFYRSCVITGTV 435
Query: 475 DFIFGHASAVFQNCQLVLRKPLDNQKNIITANGRIDSKSNTAFVLQKCVIKGEDDL---P 531
DFIFG A++VFQNC + +RKPL+NQ+NI+TA GRID T VLQ C I+ + DL
Sbjct: 436 DFIFGDATSVFQNCLITVRKPLENQQNIVTAQGRIDGHETTGIVLQSCRIEPDKDLVPVK 495
Query: 532 STTKNYIGRPWKEYSRTIIMESDIPALIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTD 591
+ ++Y+GRPWKE+SRT+IM+S I I P GWLPW+GDF LKTLYY EY N G GA+T+
Sbjct: 496 NKIRSYLGRPWKEFSRTVIMDSTIGDFIHPGGWLPWQGDFGLKTLYYAEYSNKGGGAQTN 555
Query: 592 ARVKWIGRKDIKRGEALTYTVEPFLDGSWINGTGVPAHLGL 632
AR+KW G IK+ EA+ +T+E F G WI+ +G P HLGL
Sbjct: 556 ARIKWPGYHIIKKEEAMKFTIENFYQGDWISASGSPVHLGL 596
>emb|CAB80040.1| pectinesterase-like protein [Arabidopsis thaliana]
gi|4455337|emb|CAB36797.1| pectinesterase-like protein
[Arabidopsis thaliana] gi|15234112|ref|NP_195049.1|
pectinesterase family protein [Arabidopsis thaliana]
gi|7447390|pir||T05203 pectinesterase homolog F4I10.160
- Arabidopsis thaliana
Length = 609
Score = 494 bits (1271), Expect = e-138
Identities = 271/661 (40%), Positives = 375/661 (55%), Gaps = 80/661 (12%)
Query: 1 MAFEDFDLVSERRKANAKQHLKKKILIGVTSVVLIACVIAAVTFVIV------KRSGPDH 54
MAF+DFD + ER A K+ +K+I++GV SV+++A I F V + G
Sbjct: 1 MAFQDFDKIQERVNAERKRKFRKRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTT 60
Query: 55 NNNDK-KPVQNAPPEPE---------RVDKYSRLVTMLCSHSEYKEKCVTTLKEALKKDP 104
NN K P ++ P P+ + + +++ LC+ + YK C TLK KKD
Sbjct: 61 NNKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDT 120
Query: 105 KLKEPKGLLMVFMLVAKNEINNAFNKTANLKFASKEEKGAYEDCKQLFEDAKEEMGFSIT 164
+P+ LL ++ ++++ F + +LK +K++K A CK L ++AKEE+G S+
Sbjct: 121 PQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKTENKDDKDAIAQCKLLVDEAKEELGTSMK 180
Query: 165 EVGQLDISKLASKEAELNNWLSAVISYQDTCSDGFPEGELKKKMEMIFAESRQLLSNSLA 224
+ +++ A +L++WLSAV+SYQ+TC DGF EG+LK ++ F S+ L SNSLA
Sbjct: 181 RINDSEVNNFAKIVPDLDSWLSAVMSYQETCVDGFEEGKLKTEIRKNFNSSQVLTSNSLA 240
Query: 225 VVSQVSQIVNAFQGGLSGFKLPWGKSDAHAPAPDADTDAVADDDEDLADAPDGAPDADQP 284
+ + + G LS P T + +
Sbjct: 241 M-------IKSLDGYLSS-------------VPKVKTRLLLE------------------ 262
Query: 285 IFEAPIGAPGAAPIGAPRVDAPPSWAA-------PAVDLPGSTEKPTPNVTVAKDGSGDF 337
A A D SW + AVD+ PN TVAKDGSG+F
Sbjct: 263 -----------ARSSAKETDHITSWLSNKERRMLKAVDVKALK----PNATVAKDGSGNF 307
Query: 338 KTISEALAAIPQTYKGRYVVYVKEGVYDETVTVTKKMVNLTMYGDGGLKSIITGNKNFVD 397
TI+ AL A+P Y+GRY +Y+K G+YDE+V + KK N+TM GDG K+I+TGNK+
Sbjct: 308 TTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAK 367
Query: 398 GVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQADCSIFVNCNFEGYQDTLY 457
+RTF TA+FV G+GF+ + MGFRNTAG QAVA RVQ+D S+F+NC FEGYQDTLY
Sbjct: 368 KIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLY 427
Query: 458 AQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQKNIITANGRIDSKSNTAF 517
A THRQ+YR CVI GT+DFIFG A+A+FQNC + +RK L QKN +TA GR+D T F
Sbjct: 428 AYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGF 487
Query: 518 VLQKCVIKGEDDL---PSTTKNYIGRPWKEYSRTIIMESDIPALIQPEGWLPW-EGDFAL 573
V+ C + +DL + K+Y+GRPWK +SRT++MES I +I P GWL W E DFA+
Sbjct: 488 VIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAI 547
Query: 574 KTLYYGEYDNVGAGAKTDARVKWIGRKDIKRGEALTYTVEPFLDGSWINGTGVPAHLGLY 633
TL Y EY N G T ARVKW G + + + EA+ +TV PFL G WI G P LGLY
Sbjct: 548 DTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGEWIQAIGSPVKLGLY 607
Query: 634 N 634
+
Sbjct: 608 D 608
>dbj|BAD33558.1| putative pectinesterase [Oryza sativa (japonica cultivar-group)]
Length = 617
Score = 484 bits (1247), Expect = e-135
Identities = 270/648 (41%), Positives = 381/648 (58%), Gaps = 63/648 (9%)
Query: 2 AFEDFDLVSERRKANAKQHLKKKILI--GVTSVVLIACVIAAVTFVIVKRSGPDHNNNDK 59
AF DF ++ERR+A + +++I+I G S+++I V+ A + + K
Sbjct: 4 AFGDFGPLTERRRAEKARQQRRRIMIALGTVSIIIILIVMGAAAITYSGKKSEEDEGGSK 63
Query: 60 KPVQNAPPEPERVDK--------------YSRLVTMLCSHSEYKEKCVTTLKEALKKDPK 105
+ D S+ + M+C+ +++ + C T++ +A +
Sbjct: 64 GSSKGKSKGGGGGDDEDGGGGGGKADLRAVSKSIKMMCAQTDFADSCATSIGKAA--NAS 121
Query: 106 LKEPKGLLMVFMLVAKNEINNAFNKTANLKFASKEEKGAYEDCKQLFEDAKEEMGFSITE 165
+ PK ++ + V ++ AF++ + K A DCK+LF+DAK+++ ++
Sbjct: 122 VSSPKDIIRTAVDVIGGAVDQAFDRADLIMSNDPRVKAAVADCKELFDDAKDDLNCTLKG 181
Query: 166 VGQLDISKLASKEAELNNWLSAVISYQDTCSDGFPEGELKKKMEMIFAESRQLLSNSLAV 225
+ D K + +L WLSAVI+ +TC DGFP+GE + K++ F R+ SN+LA+
Sbjct: 182 IDGKDGLK---QGFQLRVWLSAVIANMETCIDGFPDGEFRDKVKESFNNGREFTSNALAL 238
Query: 226 VSQVSQIVNAFQGGLSGFKLPWGKSDAHAPAPDADTDAVADDDEDLADAPDGAPDADQPI 285
+ + S ++A +G S +L G+ D A D A+A+D P+ PD D+ +
Sbjct: 239 IEKASSFLSALKG--SQRRLLAGEEDNGGGAADPHL-ALAEDG-----IPEWVPDGDRRV 290
Query: 286 FEAPIGAPGAAPIGAPRVDAPPSWAAPAVDLPGSTEKPTPNVTVAKDGSGDFKTISEALA 345
+ G TPNV VAKDGSG FKTI+EALA
Sbjct: 291 LKGG----------------------------GFKNNLTPNVIVAKDGSGKFKTINEALA 322
Query: 346 AIPQTYKGRYVVYVKEGVYDETVTVTKKMVNLTMYGDGGLKSIITGNKNFVDGVRTFQTA 405
A+P+TY GRYV+YVKEGVY E VT+TKKM ++TMYGDG KSI+TG+KNF DG+ TF+TA
Sbjct: 323 AMPKTYSGRYVIYVKEGVYAEYVTITKKMASVTMYGDGSRKSIVTGSKNFADGLTTFKTA 382
Query: 406 SFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQADCSIFVNCNFEGYQDTLYAQTHRQFY 465
+F GDGF+ MGF+NTAGA K QAVA VQ+D S+F+NC +G+QDTLYA + QFY
Sbjct: 383 TFAAQGDGFMAIGMGFQNTAGAAKHQAVALLVQSDKSVFLNCWMDGFQDTLYAHSKAQFY 442
Query: 466 RDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQKNIITANGRIDSKSNTAFVLQKCVIK 525
R+CVI+GTIDF+FG A+AVFQNC L LR+P+DNQ+NI TA GR D + T FVLQKC
Sbjct: 443 RNCVITGTIDFVFGDAAAVFQNCVLTLRRPMDNQQNIATAQGRADGREATGFVLQKCEFN 502
Query: 526 GEDDLPST----TKNYIGRPWKEYSRTIIMESDIPALIQPEGWLPWEGDFALKTLYYGEY 581
E L +NY+GRPW+E+SRT+IMESDIPA+I G++PW G+FALKTLYY EY
Sbjct: 503 AEPALTDAKLPPIRNYLGRPWREFSRTVIMESDIPAIIDKAGYMPWNGEFALKTLYYAEY 562
Query: 582 DNVGAGAKTDARVKWIG-RKDIKRGEALTYTVEPFLDGS-WINGTGVP 627
N G GA T RV W G +K I + +A +TV+ FL WI+ TG P
Sbjct: 563 ANKGPGADTAGRVAWPGYKKVISKADATKFTVDNFLHAKPWIDPTGTP 610
>gb|AAP40488.1| putative pectin methylesterase [Arabidopsis thaliana]
Length = 614
Score = 466 bits (1200), Expect = e-130
Identities = 273/668 (40%), Positives = 369/668 (54%), Gaps = 89/668 (13%)
Query: 1 MAFEDFDLVSERRKANAKQHLKKKILIGVTSVVLIACVIAAVTFVIV------------- 47
MAF+DFD + ER AN K+ +K+I++G S++++ I F V
Sbjct: 1 MAFQDFDKIQERVNANRKRKFRKRIIVGTVSLLVVVAAIVGGAFAYVAYEKRNEQQQQQQ 60
Query: 48 -----KRSGPDHN---NNDKK------PVQNAP---PEPERVDKYSRLVTMLCSHSEYKE 90
+SG +N ++DKK P Q AP + + + +++ LCS + Y +
Sbjct: 61 QAKNHNKSGSGNNVVKDSDKKSPSPPTPSQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQ 120
Query: 91 KCVTTLKEALKKDPKLKEPKGLLMVFMLVAKNEINNAFNKTANLKFASKEEKGAYEDCKQ 150
C TLK K L P L + +++ K +LK ++++K A E CK
Sbjct: 121 ICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKTENQDDKDAIEQCKL 180
Query: 151 LFEDAKEEMGFSITEVGQLDISKLASKEAELNNWLSAVISYQDTCSDGFPEGELKKKMEM 210
L EDAKEE S+ ++ +++ +L +WLSAV+SYQ+TC DGF EG LK +++
Sbjct: 181 LVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQETCLDGFEEGNLKSEVKT 240
Query: 211 IFAESRQLLSNSLAVVSQVSQIVNAFQGGLSGFKLPWGKSDAHAPAPDADTDAVADDDED 270
S+ L SNSLA++ ++ ++ + L D V++DD
Sbjct: 241 SVNSSQVLTSNSLALIKTFTENLSPVMKVVERHLL------------DGIPSWVSNDDRR 288
Query: 271 LADAPDGAPDADQPIFEAPIGAPGAAPIGAPRVDAPPSWAAPAVDLPGSTEKPTPNVTVA 330
+ A D V A A A D GS + T N
Sbjct: 289 MLRAVD--------------------------VKALKPNATVAKD--GSGDFTTIN---- 316
Query: 331 KDGSGDFKTISEALAAIPQTYKGRYVVYVKEGVYDETVTVTKKMVNLTMYGDGGLKSIIT 390
+AL A+P+ Y+GRY++YVK+G+YDE VTV KK NLTM GDG K+I+T
Sbjct: 317 -----------DALRAMPEKYEGRYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVT 365
Query: 391 GNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQADCSIFVNCNFE 450
GNK+ +RTF TA+FV G+GF+ + MGFRNTAG+ QAVA RVQ+D SIF+NC FE
Sbjct: 366 GNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGSEGHQAVAIRVQSDRSIFLNCRFE 425
Query: 451 GYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQKNIITANGRID 510
GYQDTLYA THRQ+YR CVI GTIDFIFG A+A+FQNC + +RK L QKN +TA GR+D
Sbjct: 426 GYQDTLYAYTHRQYYRSCVIVGTIDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVD 485
Query: 511 SKSNTAFVLQKCVIKGEDDLPSTT---KNYIGRPWKEYSRTIIMESDIPALIQPEGWLPW 567
T FV+ C I +DL K+Y+GRPWK YSRTIIMES I +I P GWL W
Sbjct: 486 KFQTTGFVVHNCKIAANEDLKPVKEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRW 545
Query: 568 -EGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIKRGEALTYTVEPFLDGSWINGTGV 626
E DFA+ TLYY EY+N G+ T +RVKW G K I + EAL YTV PFL G WI+ +G
Sbjct: 546 QETDFAIDTLYYAEYNNKGSSGDTTSRVKWPGFKVINKEEALNYTVGPFLQGDWISASGS 605
Query: 627 PAHLGLYN 634
P LGLY+
Sbjct: 606 PVKLGLYD 613
>emb|CAD40902.1| OSJNBa0036B21.20 [Oryza sativa (japonica cultivar-group)]
gi|50924778|ref|XP_472740.1| OSJNBa0036B21.20 [Oryza
sativa (japonica cultivar-group)]
Length = 568
Score = 413 bits (1062), Expect = e-114
Identities = 239/621 (38%), Positives = 345/621 (55%), Gaps = 66/621 (10%)
Query: 21 LKKKILIGVTSVVLIACVIAAVTFVIVKRSGPDHNNNDKKPVQNAPPEPERVDKYSRLVT 80
+ +K L+G +L+ V+ V V RSG +N P + + + V
Sbjct: 1 MAQKFLLGGVGAILVVAVVVGVV-ATVTRSGNKAGDNFTVPGE------ANLATSGKSVE 53
Query: 81 MLCSHSEYKEKCVTTLKEALKKDPKLKEPKGLLMVFMLVAKN---EINNAFNKTANL--- 134
LC+ + YKE C TL A KE VF VAK+ I +A K+ +
Sbjct: 54 SLCAPTLYKESCEKTLTTATSGTENPKE------VFSTVAKSALESIKSAVEKSKAIGEA 107
Query: 135 KFASKEEKGAYEDCKQLFEDAKEEMGFSITEVGQLDISKLASKEAELNNWLSAVISYQDT 194
K + + A EDCK L ED+ +++ + E+ D+ L S+ +L +WL+ V+++ DT
Sbjct: 108 KTSDSMTESAREDCKALLEDSVDDLR-GMVEMAGGDVKVLFSRSDDLEHWLTGVMTFMDT 166
Query: 195 CSDGFPEGELKKKMEMIFAESRQLLSNSLAVVSQVSQIVNAFQGGLSGFKLPWGKSDAHA 254
C+DGF + +LK M + + +L SN+LA+ + + I L FK G++ H
Sbjct: 167 CADGFADEKLKADMHSVLRNASELSSNALAITNTLGAIFKKLD--LDMFK---GENPIHR 221
Query: 255 PAPDADTDAVADDDEDLADAPDGAPDADQPIFEAPIGAPGAAPIGAPRVDAPPSWAAPAV 314
++ + E + P D+ + +
Sbjct: 222 --------SLIAEQETVGGFPSWMKAPDRKLLAS-------------------------- 247
Query: 315 DLPGSTEKPTPNVTVAKDGSGDFKTISEALAAIPQTYKGRYVVYVKEGVYDETVTVTKKM 374
G +P PN VA+DGSG FKTI EA+ ++P+ ++GRYV+YVK G+YDE V V K
Sbjct: 248 ---GDRNRPQPNAVVAQDGSGQFKTIQEAVNSMPKGHQGRYVIYVKAGLYDEIVMVPKDK 304
Query: 375 VNLTMYGDGGLKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVA 434
VN+ MYGDG +S +TG K+F DG+ T +TA+F V GF+ ++MGF NTAGA + QAVA
Sbjct: 305 VNIFMYGDGPKRSRVTGRKSFADGITTMKTATFSVEAAGFICKNMGFHNTAGAERHQAVA 364
Query: 435 ARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRK 494
R+ D F NC F+ +QDTLY RQF+R+CVISGTIDFIFG+++AVFQNC ++ R+
Sbjct: 365 LRINGDLGAFYNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSAAVFQNCLIITRR 424
Query: 495 PLDNQKNIITANGRIDSKSNTAFVLQKCVIKGEDDL-PSTTK--NYIGRPWKEYSRTIIM 551
P+DNQ+N +TA+GR D + V+Q C + + L P K +Y+GRPWKEYSR +IM
Sbjct: 425 PMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKLFPDRFKIPSYLGRPWKEYSRLVIM 484
Query: 552 ESDIPALIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIKRGEALTYT 611
ES I I+PEG++PW G+FAL TLYY E++N G GA T RV W G + I + EA +T
Sbjct: 485 ESTIADFIKPEGYMPWNGEFALNTLYYAEFNNRGPGAGTSKRVNWKGFRVIGQKEAEQFT 544
Query: 612 VEPFLD-GSWINGTGVPAHLG 631
PF+D G+W+ TG P LG
Sbjct: 545 AGPFVDGGTWLKFTGTPHFLG 565
>ref|NP_198139.1| pectinesterase family protein [Arabidopsis thaliana]
Length = 732
Score = 399 bits (1024), Expect = e-109
Identities = 233/622 (37%), Positives = 343/622 (54%), Gaps = 80/622 (12%)
Query: 22 KKKILIGVTSVVLIACVIAAVTFVIVKRSGPDHNNNDKKPVQNAPPEPERVDKYSRLVTM 81
K+ ++I ++SV+LI+ V+A V V +S N D E + + +
Sbjct: 14 KRYVIISISSVLLISMVVAVTIGVSVNKSD---NAGD-----------EEITTSVKAIKD 59
Query: 82 LCSHSEYKEKCVTTLKEALKKDPK-LKEPKGLLMVFMLVAKNEINNAFNKTANLKFASKE 140
+C+ ++YKE C ++ L+KD K +P L+ +I++ K+ + K+
Sbjct: 60 VCAPTDYKETC----EDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKD 115
Query: 141 EKG--AYEDCKQLFEDAKEEMGFSITEVGQLDISKLASKEAELNNWLSAVISYQDTCSDG 198
+ A + CK+L + A E+ S E+G+ + K+ +L WLSA IS++ TC DG
Sbjct: 116 PRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDG 175
Query: 199 F--PEGELKKKMEMIFAESRQLLSNSLAVVSQVSQIVNAFQGGLSGFKLPWGKSDAHAPA 256
F +G + ++ + QL N LA+V+++S + Q
Sbjct: 176 FQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQ------------------I 217
Query: 257 PDADTDAVADDDEDLADAPDGAPDADQPIFEAPIGAPGAAPIGAPRVDAPPSWAAPAVDL 316
P+ ++ + + F + + A + AP + P
Sbjct: 218 PEMNSRRLLSQE-----------------FPSWMDARARRLLNAPMSEVKP--------- 251
Query: 317 PGSTEKPTPNVTVAKDGSGDFKTISEALAAIPQTYKGRYVVYVKEGVYDETVTVTKKMVN 376
++ VA+DGSG +KTI+EAL +P+ +VV++KEG+Y E V V + M +
Sbjct: 252 ---------DIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMTH 302
Query: 377 LTMYGDGGLKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAAR 436
L GDG K++I+G+K++ DG+ T++TA+ ++GD F+ +++ F NTAGAIK QAVA R
Sbjct: 303 LVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVAIR 362
Query: 437 VQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPL 496
V AD SIF NC F+GYQDTLYA +HRQFYRDC ISGTIDF+FG A+AVFQNC L++RKPL
Sbjct: 363 VLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRKPL 422
Query: 497 DNQKNIITANGRIDSKSNTAFVLQKCVIKGEDD---LPSTTKNYIGRPWKEYSRTIIMES 553
NQ ITA+GR D + +T FVLQ C I GE D + +K Y+GRPWKEYSRTIIM +
Sbjct: 423 LNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIMNT 482
Query: 554 DIPALIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIKRGEALTYTVE 613
IP + PEGW PW G+F L TL+Y E N G GA RV W G K + E L +T
Sbjct: 483 FIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFTPA 542
Query: 614 PFLDG-SWINGTGVPAHLGLYN 634
++ G +WI G GVP LGL++
Sbjct: 543 QYIQGDAWIPGKGVPYILGLFS 564
>ref|XP_470886.1| putative pectin methylesterase [Oryza sativa (japonica
cultivar-group)] gi|30017519|gb|AAP12941.1| putative
pectin methylesterase [Oryza sativa (japonica
cultivar-group)]
Length = 603
Score = 395 bits (1015), Expect = e-108
Identities = 238/630 (37%), Positives = 353/630 (55%), Gaps = 65/630 (10%)
Query: 26 LIGVTSVVLIACVIAAVTFVIVKRSGPDHNNNDKKPVQNAPPEPERVDKYSRLVTMLCSH 85
LI V+L+A V+ V +V+V R+G D + K+ +++ R V + C+
Sbjct: 15 LIIAAVVMLLAIVLGTVAYVVVDRAGDD-DGLSKRGMKST----------MRSVDLFCAP 63
Query: 86 SEYKEKCVTTLKEALKK--DPKLKEPKGLLMVFMLVAKNEINNAFNKTANLKF--ASKEE 141
++Y+ C TL+ L + DP P + + E+ F++++ L+ AS +
Sbjct: 64 TDYRVACKDTLERVLARSSDPA-DHPHAAAAAAITAVERELARGFDRSSVLEAVRASNDS 122
Query: 142 K--GAYEDCKQLFEDAKEEMGFSITEVGQLDISKLASKEAELNNWLSAVISYQDTCSDGF 199
+ A DC+ L D + ++ ++T + + ++ +L WLSAVI++Q +C D F
Sbjct: 123 RVAEALRDCRTLLGDCRGDVSRALTSIAWRGVDAVSQ---DLQAWLSAVITFQGSCVDMF 179
Query: 200 PEGELKKKMEMIFAESRQLLSNSLAVVSQVSQIVNAFQGGLSGFKLPWGKSDAHAPAPDA 259
P+G +K ++ ++R++ SN++A++ Q + AF L D HA A
Sbjct: 180 PQGPIKDQVREAMEKAREISSNAIAIIQQGA----AFAAML----------DLHASESHA 225
Query: 260 DTDAVADDDEDLADAPDGAPDADQPIFEAPIGAPGAAPIGAPRVDAPPSWAAPAVDLPGS 319
D D D+ D + DQ + P P + + + + A
Sbjct: 226 AEGEELDVDHDIQHHVDRHLE-DQSLPPVP---PWLSDEDRRMLTSGEEFVAGL------ 275
Query: 320 TEKPTPNVTVAKDGSGDFKTISEALAAIPQTYKGRYVVYVKEGVYDETVTVTKKMVNLTM 379
TPNVTVAKDGSGDF IS AL A+P+ Y G+Y++YVKEGVYDETV VT +M N+TM
Sbjct: 276 ----TPNVTVAKDGSGDFTNISAALDALPEAYAGKYIIYVKEGVYDETVNVTSRMANITM 331
Query: 380 YGDGGLKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQA 439
YGDG KSI+TG+KN DGVR ++TA+F V GD F +G RNTAG K+QA+A RV+A
Sbjct: 332 YGDGSKKSIVTGSKNIADGVRMWKTATFAVDGDRFTAMRLGIRNTAGEEKQQALALRVKA 391
Query: 440 DCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQ 499
D SIF NC EG QDTL+AQ +RQFYR CVISGT+DFIFG A+A+FQ C ++++ PL +
Sbjct: 392 DKSIFFNCRIEGNQDTLFAQAYRQFYRSCVISGTVDFIFGDAAAMFQRCIILVKPPLPGK 451
Query: 500 KNIITANGRIDSKSNTAFVLQKCVIKGEDDL------PSTTKN---------YIGRPWKE 544
++TA+GR D + T FVL + ++D S T + Y+GRPWKE
Sbjct: 452 PAVVTAHGRRDRQQTTGFVLHHSQVVADEDFAGAGGGSSNTSSSSGAAPRLAYLGRPWKE 511
Query: 545 YSRTIIMESDIPALIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIKR 604
++RTI+MES I + +G++PWEG L +YGEY N G GA + R++ G + R
Sbjct: 512 HARTIVMESVIGGFVHAQGYMPWEGKDNLGEAFYGEYGNSGQGANSTGRMEMRGFHVLDR 571
Query: 605 GEALTYTVEPFLDGS-WINGTGVPAHLGLY 633
+A+ +TV FL G+ WI TG P +GL+
Sbjct: 572 EKAMQFTVGRFLHGADWIPETGTPVTIGLF 601
>emb|CAA73733.1| pectin methylesterase-like protein [Zea mays]
gi|7447384|pir||T04359 pectin methylesterase-like
protein - maize
Length = 563
Score = 392 bits (1007), Expect = e-107
Identities = 210/495 (42%), Positives = 295/495 (59%), Gaps = 53/495 (10%)
Query: 142 KGAYEDCKQLFEDAKEEMGFSITEVGQLDISKLASKEAELNNWLSAVISYQDTCSDGFPE 201
+ A EDCK+L EDA +++ + E+ DI L S+ +L WL+ V+++ DTC DGF +
Sbjct: 114 ESAREDCKKLLEDAADDLR-GMLEMAGGDIKVLFSRSDDLETWLTGVMTFMDTCVDGFVD 172
Query: 202 GELKKKMEMIFAESRQLLSNSLAVVSQVSQIVNAFQGGLSGFKLPWGKSDAHAPAPDADT 261
+LK M + + +L SN+LA+ + + I+ K D + D+
Sbjct: 173 EKLKADMHSVVRNATELSSNALAITNSLGGILK--------------KMDLGMFSKDSRR 218
Query: 262 DAVADDDEDLADAPDGAPDADQPIFEAPIGAPGAAPIGAPRVDAPPSWA-APAVDLPGST 320
++ + ++ P W +P L S
Sbjct: 219 RLLSSEQDE---------------------------------KGWPVWMRSPERKLLASG 245
Query: 321 EKPTPNVTVAKDGSGDFKTISEALAAIPQTYKGRYVVYVKEGVYDETVTVTKKMVNLTMY 380
+P PN VAKDGSG FK+I +A+ A+P+ ++GRYV+YVK G+YDE V V K VN+ MY
Sbjct: 246 NQPKPNAIVAKDGSGQFKSIQQAVDAVPKGHQGRYVIYVKAGLYDEIVMVPKDKVNIFMY 305
Query: 381 GDGGLKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQAD 440
GDG +S +TG K+F DG+ T +TA+F V GF+ ++MGF NTAGA + QAVA RVQ D
Sbjct: 306 GDGPKQSRVTGRKSFADGITTMKTATFSVEASGFICKNMGFHNTAGAERHQAVALRVQGD 365
Query: 441 CSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQK 500
+ F NC F+ +QDTLY Q RQF+R+CV+SGTIDFIFG+++AVFQNC ++ R+P+DNQ+
Sbjct: 366 LAAFYNCRFDAFQDTLYVQPRRQFFRNCVVSGTIDFIFGNSAAVFQNCLIITRRPMDNQQ 425
Query: 501 NIITANGRIDSKSNTAFVLQKCVIKGEDDL-PSTTK--NYIGRPWKEYSRTIIMESDIPA 557
N +TA+G D + V+Q C + + L P K +Y+GRPWKE+SR +IMES I
Sbjct: 426 NSVTAHGPTDPNMKSGLVIQNCRLVPDQKLFPDRFKIPSYLGRPWKEFSRLVIMESTIAD 485
Query: 558 LIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIKRGEALTYTVEPFLD 617
++PEG++PW GDFALKTLYY EY+N G GA T RV W G I R EA +T PF+D
Sbjct: 486 FVKPEGYMPWNGDFALKTLYYAEYNNRGPGAGTSKRVNWPGFHVIGRKEAEPFTAGPFID 545
Query: 618 GS-WINGTGVPAHLG 631
G+ W+ TG P LG
Sbjct: 546 GAMWLKYTGAPHILG 560
>ref|NP_850077.1| pectinesterase family protein [Arabidopsis thaliana]
Length = 614
Score = 391 bits (1005), Expect = e-107
Identities = 197/343 (57%), Positives = 239/343 (69%), Gaps = 15/343 (4%)
Query: 303 VDAPPSWAAP-------AVDLPGSTEKPTPNVTVAKDGSGDFKTISEALAAIPQTYKGRY 355
+D PSW + AVD+ PN TVAKDGSGDF TI++AL A+P+ Y+GRY
Sbjct: 275 LDDIPSWVSNDDRRMLRAVDVKALK----PNATVAKDGSGDFTTINDALRAMPEKYEGRY 330
Query: 356 VVYVKEGVYDETVTVTKKMVNLTMYGDGGLKSIITGNKNFVDGVRTFQTASFVVLGDGFV 415
++YVK+G+YDE VTV KK NLTM GDG K+I+TGNK+ +RTF TA+FV G+GF+
Sbjct: 331 IIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFM 390
Query: 416 GRDMGFRNTAGAIKEQAVAARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTID 475
+ MGFRNTAG QAVA RVQ+D SIF+NC FEGYQDTLYA THRQ+YR CVI GTID
Sbjct: 391 AQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGTID 450
Query: 476 FIFGHASAVFQNCQLVLRKPLDNQKNIITANGRIDSKSNTAFVLQKCVIKGEDDLPSTT- 534
FIFG A+A+FQNC + +RK L QKN +TA GR+D T FV+ C I +DL
Sbjct: 451 FIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPVKE 510
Query: 535 --KNYIGRPWKEYSRTIIMESDIPALIQPEGWLPW-EGDFALKTLYYGEYDNVGAGAKTD 591
K+Y+GRPWK YSRTIIMES I +I P GWL W E DFA+ TLYY EY+N G+ T
Sbjct: 511 EYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGDTT 570
Query: 592 ARVKWIGRKDIKRGEALTYTVEPFLDGSWINGTGVPAHLGLYN 634
+RVKW G K I + EAL YTV PFL G WI+ +G P LGLY+
Sbjct: 571 SRVKWPGFKVINKEEALNYTVGPFLQGDWISASGSPVKLGLYD 613
Score = 132 bits (331), Expect = 4e-29
Identities = 81/261 (31%), Positives = 131/261 (50%), Gaps = 30/261 (11%)
Query: 1 MAFEDFDLVSERRKANAKQHLKKKILIGVTSVVLIACVIAAVTFVIV------------- 47
MAF+DFD + ER AN K+ +K+I++G S++++ I F V
Sbjct: 1 MAFQDFDKIQERVNANRKRKFRKRIIVGTVSLLVVVAAIVGGAFAYVAYEKRNEQQQQQQ 60
Query: 48 -----KRSGPDHN---NNDKK------PVQNAP---PEPERVDKYSRLVTMLCSHSEYKE 90
+SG +N ++DKK P Q AP + + + +++ LCS + Y +
Sbjct: 61 QAKNHNKSGSGNNVVKDSDKKSPSPPTPSQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQ 120
Query: 91 KCVTTLKEALKKDPKLKEPKGLLMVFMLVAKNEINNAFNKTANLKFASKEEKGAYEDCKQ 150
C TLK K L P L + +++ K +LK ++++K A E CK
Sbjct: 121 ICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKTENQDDKDAIEQCKL 180
Query: 151 LFEDAKEEMGFSITEVGQLDISKLASKEAELNNWLSAVISYQDTCSDGFPEGELKKKMEM 210
L EDAKEE S+ ++ +++ +L +WLSAV+SYQ+TC DGF EG LK +++
Sbjct: 181 LVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQETCLDGFEEGNLKSEVKT 240
Query: 211 IFAESRQLLSNSLAVVSQVSQ 231
S+ L SNSLA++ ++
Sbjct: 241 SVNSSQVLTSNSLALIKTFTE 261
>gb|AAC14494.1| putative pectinesterase [Arabidopsis thaliana]
gi|7447376|pir||T00978 probable pectinesterase (EC
3.1.1.11) At2g26450 [imported] - Arabidopsis thaliana
Length = 496
Score = 391 bits (1005), Expect = e-107
Identities = 197/343 (57%), Positives = 239/343 (69%), Gaps = 15/343 (4%)
Query: 303 VDAPPSWAAP-------AVDLPGSTEKPTPNVTVAKDGSGDFKTISEALAAIPQTYKGRY 355
+D PSW + AVD+ PN TVAKDGSGDF TI++AL A+P+ Y+GRY
Sbjct: 157 LDDIPSWVSNDDRRMLRAVDVKALK----PNATVAKDGSGDFTTINDALRAMPEKYEGRY 212
Query: 356 VVYVKEGVYDETVTVTKKMVNLTMYGDGGLKSIITGNKNFVDGVRTFQTASFVVLGDGFV 415
++YVK+G+YDE VTV KK NLTM GDG K+I+TGNK+ +RTF TA+FV G+GF+
Sbjct: 213 IIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFM 272
Query: 416 GRDMGFRNTAGAIKEQAVAARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTID 475
+ MGFRNTAG QAVA RVQ+D SIF+NC FEGYQDTLYA THRQ+YR CVI GTID
Sbjct: 273 AQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGTID 332
Query: 476 FIFGHASAVFQNCQLVLRKPLDNQKNIITANGRIDSKSNTAFVLQKCVIKGEDDLPSTT- 534
FIFG A+A+FQNC + +RK L QKN +TA GR+D T FV+ C I +DL
Sbjct: 333 FIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPVKE 392
Query: 535 --KNYIGRPWKEYSRTIIMESDIPALIQPEGWLPW-EGDFALKTLYYGEYDNVGAGAKTD 591
K+Y+GRPWK YSRTIIMES I +I P GWL W E DFA+ TLYY EY+N G+ T
Sbjct: 393 EYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGDTT 452
Query: 592 ARVKWIGRKDIKRGEALTYTVEPFLDGSWINGTGVPAHLGLYN 634
+RVKW G K I + EAL YTV PFL G WI+ +G P LGLY+
Sbjct: 453 SRVKWPGFKVINKEEALNYTVGPFLQGDWISASGSPVKLGLYD 495
Score = 92.8 bits (229), Expect = 3e-17
Identities = 49/140 (35%), Positives = 76/140 (54%)
Query: 92 CVTTLKEALKKDPKLKEPKGLLMVFMLVAKNEINNAFNKTANLKFASKEEKGAYEDCKQL 151
C TLK K L P L + +++ K +LK ++++K A E CK L
Sbjct: 4 CEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKTENQDDKDAIEQCKLL 63
Query: 152 FEDAKEEMGFSITEVGQLDISKLASKEAELNNWLSAVISYQDTCSDGFPEGELKKKMEMI 211
EDAKEE S+ ++ +++ +L +WLSAV+SYQ+TC DGF EG LK +++
Sbjct: 64 VEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQETCLDGFEEGNLKSEVKTS 123
Query: 212 FAESRQLLSNSLAVVSQVSQ 231
S+ L SNSLA++ ++
Sbjct: 124 VNSSQVLTSNSLALIKTFTE 143
>gb|AAM65978.1| pectin methylesterase [Arabidopsis thaliana]
gi|10177147|dbj|BAB10336.1| pectin methylesterase
[Arabidopsis thaliana] gi|18700151|gb|AAL77687.1|
AT5g49180/K21P3_5 [Arabidopsis thaliana]
gi|15239814|ref|NP_199729.1| pectinesterase family
protein [Arabidopsis thaliana]
gi|25090082|gb|AAN72223.1| At5g49180/K21P3_5
[Arabidopsis thaliana]
Length = 571
Score = 389 bits (999), Expect = e-106
Identities = 237/618 (38%), Positives = 335/618 (53%), Gaps = 74/618 (11%)
Query: 22 KKKILIGVTSVVLIACVIAAVTFVIVKRSGPDHNNNDKKPVQNAPPEPERVDKYSRLVTM 81
KK I+ GV + +L+ V+A V + + ++ +K V P ++ + V
Sbjct: 10 KKCIIAGVITALLVLMVVA------VGITTSRNTSHSEKIV------PVQIKTATTAVEA 57
Query: 82 LCSHSEYKEKCVTTLKEALKKDPKLKEPKGLLMVFMLVAKNEINNAFNKTA---NLKFAS 138
+C+ ++YKE CV +L +K P +P L+ + V I ++ K + K A+
Sbjct: 58 VCAPTDYKETCVNSL---MKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAAN 114
Query: 139 -KEEKGAYEDCKQLFEDAKEEMGFSITEVGQLDISKLASKEAELNNWLSAVISYQDTCSD 197
K+ KGA E C++L DA +++ + I ++ +L WLS I+YQ TC D
Sbjct: 115 DKDTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMD 174
Query: 198 GFPE--GELKKKMEMIFAESRQLLSNSLAVVSQVSQIVNAFQGGLSGFKLPWGKSDAHAP 255
F E +L + M+ IF SR+L SN LA+++ +S ++ F ++G GK
Sbjct: 175 TFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFN--VTGVTGDLGKYA---- 228
Query: 256 APDADTDAVADDDEDLADAPDGAPDADQPIFEAPIGAPGAAPIGAPRVDAPPSWAAPAVD 315
L A DG P SW P
Sbjct: 229 -------------RKLLSAEDGIP----------------------------SWVGPNTR 247
Query: 316 LPGSTEKPTP-NVTVAKDGSGDFKTISEALAAIPQTYKGRYVVYVKEGVYDETVTVTKKM 374
+T+ NV VA DGSG +KTI+EAL A+P+ + +V+Y+K+GVY+E V VTKKM
Sbjct: 248 RLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDVTKKM 307
Query: 375 VNLTMYGDGGLKSIITGNKNFVDG-VRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAV 433
++T GDG K+ ITG+ N+ G V+T+ TA+ + GD F +++GF NTAG QAV
Sbjct: 308 THVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEGHQAV 367
Query: 434 AARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLR 493
A RV AD ++F NC +GYQDTLY +HRQF+RDC +SGT+DFIFG V QNC +V+R
Sbjct: 368 ALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVVR 427
Query: 494 KPLDNQKNIITANGRIDSKSNTAFVLQKCVIKGED---DLPSTTKNYIGRPWKEYSRTII 550
KP+ +Q +ITA GR D + +T VLQ C I GE + S K Y+GRPWKE+SRTII
Sbjct: 428 KPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFSRTII 487
Query: 551 MESDIPALIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIKRGEALTY 610
M + I +I P GWLPW GDFAL TLYY EY+N G G+ RVKW G K + +AL +
Sbjct: 488 MGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRF 547
Query: 611 TVEPFLDGS-WINGTGVP 627
T FL G+ WI VP
Sbjct: 548 TPARFLRGNLWIPPNRVP 565
>ref|XP_482698.1| putative pectinesterase [Oryza sativa (japonica cultivar-group)]
gi|42407617|dbj|BAD08732.1| putative pectinesterase
[Oryza sativa (japonica cultivar-group)]
Length = 664
Score = 385 bits (988), Expect = e-105
Identities = 192/318 (60%), Positives = 234/318 (73%), Gaps = 6/318 (1%)
Query: 322 KPTPNVTVAKDGSGDFKTISEALAAIPQTYKGRYVVYVKEGVYDETVTVTKKMVNLTMYG 381
K PNV VAKDGSG FKTI++ALAA+P+ Y GRYV+YVKEGVY+E VT+TKKM N+TMYG
Sbjct: 346 KLKPNVVVAKDGSGKFKTINDALAAMPKKYTGRYVIYVKEGVYEEYVTITKKMANVTMYG 405
Query: 382 DGGLKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQADC 441
DG K+IITGN+NFVDG+ T++TA+F GDGF+G +GFRNTA A K QAVA VQ+D
Sbjct: 406 DGAKKTIITGNRNFVDGLTTYKTATFNAQGDGFMGVALGFRNTARAAKHQAVALLVQSDK 465
Query: 442 SIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQKN 501
SIF+NC EG+QDTLYA + QFYR+CVISGT+DFIFG A+AVFQNC +VLR+PLDNQ+N
Sbjct: 466 SIFLNCRMEGHQDTLYAHSKAQFYRNCVISGTVDFIFGDAAAVFQNCVIVLRRPLDNQQN 525
Query: 502 IITANGRIDSKSNTAFVLQKCVIKGEDDLPSTT----KNYIGRPWKEYSRTIIMESDIPA 557
I TA GR D + T FVLQ E L + ++Y+ RPW+EYSRT+IM SDIPA
Sbjct: 526 IATAQGRADRREATGFVLQHYRFAAESALGDASRPAVRSYLARPWREYSRTLIMNSDIPA 585
Query: 558 LIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIG-RKDIKRGEALTYTVEPFL 616
+ G+LPW GDF LKTL+Y EY N GAGA T RV W G +K I + EA +TV+ FL
Sbjct: 586 FVDKAGYLPWSGDFGLKTLWYAEYGNKGAGAATAGRVSWPGYKKVISKKEATKFTVQNFL 645
Query: 617 DGS-WINGTGVPAHLGLY 633
WI TG P G++
Sbjct: 646 HAEPWIKPTGTPVKYGMW 663
Score = 118 bits (295), Expect = 7e-25
Identities = 64/238 (26%), Positives = 123/238 (50%), Gaps = 6/238 (2%)
Query: 3 FEDFDLVSERRKANAKQHLKKKILI--GVTSVVLIACVIAAVTFVIVKRSGPDHNNNDKK 60
F DFD ++ERR ++ ++++++ G SV+LI V+ G +++
Sbjct: 5 FSDFDPITERRHVERQRQERRRVMVAAGAASVILIIIVMGGAAVAYNASFGDGGSSSSSG 64
Query: 61 PVQNAPPEPERVDKYSRLVTMLCSHSEYKEKCVTTLKEALKK---DPKLKEPKGLLMVFM 117
+P + S+++ +C+ ++YK+ C +L +A PK ++ +
Sbjct: 65 SASGGGAQPS-LHGVSKIIKAMCAQTDYKDTCEKSLAKAAANASASSSSSSPKDVVRASV 123
Query: 118 LVAKNEINNAFNKTANLKFASKEEKGAYEDCKQLFEDAKEEMGFSITEVGQLDISKLASK 177
V + I AF+K++ + K A DCK+++E+AK+++ ++ + + L
Sbjct: 124 AVIGDAIEKAFDKSSVIVSDDPRVKAAVADCKEIYENAKDDLDRTLAGIDAGGVDGLTKG 183
Query: 178 EAELNNWLSAVISYQDTCSDGFPEGELKKKMEMIFAESRQLLSNSLAVVSQVSQIVNA 235
+L WLSAVI++Q+TC DGFP+G+LK KM ++L SN+LA++ + S + A
Sbjct: 184 GYQLRVWLSAVIAHQETCIDGFPDGDLKDKMRDAMESGKELTSNALALIGKASSFLAA 241
>gb|AAF26136.1| putative pectinesterase [Arabidopsis thaliana]
gi|15230019|ref|NP_187212.1| pectinesterase family
protein [Arabidopsis thaliana]
Length = 669
Score = 348 bits (894), Expect = 2e-94
Identities = 169/312 (54%), Positives = 219/312 (70%), Gaps = 4/312 (1%)
Query: 325 PNVTVAKDGSGDFKTISEALAAIPQTYKGRYVVYVKEGVYDETVTVTKKMVNLTMYGDGG 384
P++ VA+DGSG +KTI+EAL +P+ +VV++K G+Y E V V K M +L GDG
Sbjct: 254 PDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLVFIGDGP 313
Query: 385 LKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQADCSIF 444
K+II+GNKN+ DG+ T++TA+ ++G+ F+ +++GF NTAGAIK QAVA RVQ+D SIF
Sbjct: 314 DKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQSDESIF 373
Query: 445 VNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQKNIIT 504
NC F+GYQDTLY +HRQF+RDC ISGTIDF+FG A+AVFQNC L++RKPL NQ IT
Sbjct: 374 FNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPNQACPIT 433
Query: 505 ANGRIDSKSNTAFVLQKCVIKGEDD---LPSTTKNYIGRPWKEYSRTIIMESDIPALIQP 561
A+GR D + +T FV Q C I GE D + T+K Y+GRPWKEYSRTIIM + IP +QP
Sbjct: 434 AHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFIPDFVQP 493
Query: 562 EGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIKRGEALTYTVEPFLDG-SW 620
+GW PW GDF LKTL+Y E N G G+ RV W G K + + L +T ++ G W
Sbjct: 494 QGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQYIQGDDW 553
Query: 621 INGTGVPAHLGL 632
I G GVP GL
Sbjct: 554 IPGKGVPYTTGL 565
Score = 83.2 bits (204), Expect = 2e-14
Identities = 57/220 (25%), Positives = 108/220 (48%), Gaps = 19/220 (8%)
Query: 22 KKKILIGVTSVVLIACVIAAVTFVIVKRSGPDHNNNDKKPVQNAPPEPERVDKYSRLVTM 81
++ I+I ++SV+LI+ V+A V + + H+ + K + V+ + V
Sbjct: 13 RRYIVITISSVLLISMVVAVTVGVSLNK----HDGDSKGKAE--------VNASVKAVKD 60
Query: 82 LCSHSEYKEKCVTTLKEALKKDPKLKEPKGLLMVFMLVAKNEINNAFNKTANLKFASKEE 141
+C+ ++Y++ C TL +K +P L+ V +I +A K+ + K+
Sbjct: 61 VCAPTDYRKTCEDTL---IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDS 117
Query: 142 KG--AYEDCKQLFEDAKEEMGFSITEVGQLDISKLASKEAELNNWLSAVISYQDTCSDGF 199
+ A + CK+L + A +E+ S E+G+ + L L WLSA IS+++TC +GF
Sbjct: 118 RTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGF 177
Query: 200 --PEGELKKKMEMIFAESRQLLSNSLAVVSQVSQIVNAFQ 237
+G + M+ + +L N LA++S++S V Q
Sbjct: 178 QGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQ 217
>dbj|BAC42986.1| putative pectinesterase [Arabidopsis thaliana]
gi|29029008|gb|AAO64883.1| At3g05610 [Arabidopsis
thaliana]
Length = 669
Score = 345 bits (884), Expect = 3e-93
Identities = 167/312 (53%), Positives = 218/312 (69%), Gaps = 4/312 (1%)
Query: 325 PNVTVAKDGSGDFKTISEALAAIPQTYKGRYVVYVKEGVYDETVTVTKKMVNLTMYGDGG 384
P++ VA+DGSG +KTI+EAL +P+ +VV++K G+Y E V V K M +L GDG
Sbjct: 254 PDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLVFIGDGP 313
Query: 385 LKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQADCSIF 444
K+II+GNKN+ DG+ ++TA+ ++G+ F+ +++GF NTAGAIK QAVA RVQ+D SIF
Sbjct: 314 DKTIISGNKNYKDGITAYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQSDESIF 373
Query: 445 VNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQKNIIT 504
NC F+GYQ+TLY +HRQF+RDC ISGTIDF+FG A+AVFQNC L++RKPL NQ IT
Sbjct: 374 FNCRFDGYQNTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPNQACPIT 433
Query: 505 ANGRIDSKSNTAFVLQKCVIKGEDD---LPSTTKNYIGRPWKEYSRTIIMESDIPALIQP 561
A+GR D + +T FV Q C I GE D + T+K Y+GRPWKEYSRTIIM + IP +QP
Sbjct: 434 AHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFIPDFVQP 493
Query: 562 EGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIKRGEALTYTVEPFLDG-SW 620
+GW PW GDF LKTL+Y E N G G+ RV W G K + + L +T ++ G W
Sbjct: 494 QGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQYIQGDDW 553
Query: 621 INGTGVPAHLGL 632
I G GVP GL
Sbjct: 554 IPGKGVPYTTGL 565
Score = 83.2 bits (204), Expect = 2e-14
Identities = 57/220 (25%), Positives = 108/220 (48%), Gaps = 19/220 (8%)
Query: 22 KKKILIGVTSVVLIACVIAAVTFVIVKRSGPDHNNNDKKPVQNAPPEPERVDKYSRLVTM 81
++ I+I ++SV+LI+ V+A V + + H+ + K + V+ + V
Sbjct: 13 RRYIVITISSVLLISMVVAVTVGVSLNK----HDGDSKGKAE--------VNASVKAVKD 60
Query: 82 LCSHSEYKEKCVTTLKEALKKDPKLKEPKGLLMVFMLVAKNEINNAFNKTANLKFASKEE 141
+C+ ++Y++ C TL +K +P L+ V +I +A K+ + K+
Sbjct: 61 VCAPTDYRKTCEDTL---IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDS 117
Query: 142 KG--AYEDCKQLFEDAKEEMGFSITEVGQLDISKLASKEAELNNWLSAVISYQDTCSDGF 199
+ A + CK+L + A +E+ S E+G+ + L L WLSA IS+++TC +GF
Sbjct: 118 RTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGF 177
Query: 200 --PEGELKKKMEMIFAESRQLLSNSLAVVSQVSQIVNAFQ 237
+G + M+ + +L N LA++S++S V Q
Sbjct: 178 QGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQ 217
>gb|AAB57670.1| pectinesterase [Citrus sinensis]
Length = 584
Score = 337 bits (865), Expect = 5e-91
Identities = 213/636 (33%), Positives = 322/636 (50%), Gaps = 80/636 (12%)
Query: 10 SERRKANAKQHLKKKILIGVTSVVLIACVIAAVTFVIVKRSGPDHNNNDKKPVQNAPPEP 69
S + +N + KK L +++++A VI V V +++ D+ N
Sbjct: 16 SNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGN------------- 62
Query: 70 ERVDKYSRLVTMLCSHSEYKEKCVTTLKEALKKDPKLKEPKGLLMVFMLVAKNEINNAF- 128
+ + ++ CS + Y + C + + + K+ K ++ + + + + + +
Sbjct: 63 ---EPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119
Query: 129 --NKTANLKFASKEEKGAYEDCKQLFEDAKEEMGFSITEVGQLDISKLASKEAE-LNNWL 185
K +K EK A DC + ++ +E+ ++ ++ + K S+ A+ L +
Sbjct: 120 GIQKLLKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLM 179
Query: 186 SAVISYQDTCSDGFPEGELKKKMEMIFAESR----QLLSNSLAVVSQVSQIVNAFQGGLS 241
SA ++ Q TC DGF + K + ++ + ++ SN+LA++ ++
Sbjct: 180 SAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMT----------- 228
Query: 242 GFKLPWGKSDAHAPAPDADTDAVADDDEDLADAPDGAPDADQPIFEAPIGAPGAAPIGAP 301
DTD + + + D G P G
Sbjct: 229 ------------------DTDMMIMRTSNNRKLTEETSTVD--------GWPAWLSPGDR 262
Query: 302 RVDAPPSWAAPAVDLPGSTEKPTPNVTVAKDGSGDFKTISEALAAIPQTYKGRYVVYVKE 361
R+ L S+ P V G +FKT++ A+AA PQ RY++ +K
Sbjct: 263 RL------------LQSSSVTPNAVVAADGSG--NFKTVAAAVAAAPQGGTKRYIIRIKA 308
Query: 362 GVYDETVTVTKKMVNLTMYGDGGLKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGF 421
GVY E V VTKK N+ GDG ++IITG++N VDG TF++A+ V+G+GF+ RD+ F
Sbjct: 309 GVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITF 368
Query: 422 RNTAGAIKEQAVAARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHA 481
+NTAG K QAVA RV AD S F NC+ YQDTLY ++RQF+ +C+I+GT+DFIFG+A
Sbjct: 369 QNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNA 428
Query: 482 SAVFQNCQLVLRKPLDNQKNIITANGRIDSKSNTAFVLQKCVIKGEDDL---PSTTKNYI 538
+AV QNC + RKP QKN++TA GR D NT V+QK I DL + Y+
Sbjct: 429 AAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYL 488
Query: 539 GRPWKEYSRTIIMESDIPALIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIG 598
GRPWKEYSRT+IM+S I LI P GW W+G+FAL TL+YGE+ N GAGA T RVKW G
Sbjct: 489 GRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKG 548
Query: 599 RKDIKRG-EALTYTVEPFLDG-SWINGTGVPAHLGL 632
+ I EA +T F+ G SW+ TG P LGL
Sbjct: 549 FRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
>sp|O04886|PME1_CITSI Pectinesterase-1 precursor (Pectin methylesterase) (PE)
gi|2098705|gb|AAB57667.1| pectinesterase [Citrus
sinensis]
Length = 584
Score = 337 bits (864), Expect = 7e-91
Identities = 213/636 (33%), Positives = 322/636 (50%), Gaps = 80/636 (12%)
Query: 10 SERRKANAKQHLKKKILIGVTSVVLIACVIAAVTFVIVKRSGPDHNNNDKKPVQNAPPEP 69
S + +N + KK L +++++A VI V V +++ D+ N
Sbjct: 16 SNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGN------------- 62
Query: 70 ERVDKYSRLVTMLCSHSEYKEKCVTTLKEALKKDPKLKEPKGLLMVFMLVAKNEINNAF- 128
+ + ++ CS + Y + C + + + K+ K ++ + + + + + +
Sbjct: 63 ---EPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119
Query: 129 --NKTANLKFASKEEKGAYEDCKQLFEDAKEEMGFSITEVGQLDISKLASKEAE-LNNWL 185
K +K EK A DC + ++ +E+ ++ ++ + K S+ A+ L +
Sbjct: 120 GIQKLLKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLM 179
Query: 186 SAVISYQDTCSDGFPEGELKKKMEMIFAESR----QLLSNSLAVVSQVSQIVNAFQGGLS 241
SA ++ Q TC DGF + K + ++ + ++ SN+LA++ ++
Sbjct: 180 SAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMT----------- 228
Query: 242 GFKLPWGKSDAHAPAPDADTDAVADDDEDLADAPDGAPDADQPIFEAPIGAPGAAPIGAP 301
DTD + + + D G P G
Sbjct: 229 ------------------DTDMMIMRTSNNRKLTEETSTVD--------GWPAWLSPGDR 262
Query: 302 RVDAPPSWAAPAVDLPGSTEKPTPNVTVAKDGSGDFKTISEALAAIPQTYKGRYVVYVKE 361
R+ L S+ P V G +FKT++ A+AA PQ RY++ +K
Sbjct: 263 RL------------LQSSSVTPNAVVAADGSG--NFKTVAAAVAAAPQGGTKRYIIRIKA 308
Query: 362 GVYDETVTVTKKMVNLTMYGDGGLKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGF 421
GVY E V VTKK N+ GDG ++IITG++N VDG TF++A+ V+G+GF+ RD+ F
Sbjct: 309 GVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARDITF 368
Query: 422 RNTAGAIKEQAVAARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHA 481
+NTAG K QAVA RV AD S F NC+ YQDTLY ++RQF+ +C+I+GT+DFIFG+A
Sbjct: 369 QNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNA 428
Query: 482 SAVFQNCQLVLRKPLDNQKNIITANGRIDSKSNTAFVLQKCVIKGEDDL---PSTTKNYI 538
+AV QNC + RKP QKN++TA GR D NT V+QK I DL + Y+
Sbjct: 429 AAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYL 488
Query: 539 GRPWKEYSRTIIMESDIPALIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIG 598
GRPWKEYSRT+IM+S I LI P GW W+G+FAL TL+YGE+ N GAGA T RVKW G
Sbjct: 489 GRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKG 548
Query: 599 RKDIKRG-EALTYTVEPFLDG-SWINGTGVPAHLGL 632
+ I EA +T F+ G SW+ TG P LGL
Sbjct: 549 FRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
>sp|P83948|PME3_CITSI Pectinesterase-3 precursor (Pectin methylesterase 3) (PE 3)
Length = 584
Score = 335 bits (858), Expect = 3e-90
Identities = 210/631 (33%), Positives = 320/631 (50%), Gaps = 80/631 (12%)
Query: 15 ANAKQHLKKKILIGVTSVVLIACVIAAVTFVIVKRSGPDHNNNDKKPVQNAPPEPERVDK 74
+N + KK L +++++A VI V V +++ D+ N +
Sbjct: 21 SNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGN----------------EP 64
Query: 75 YSRLVTMLCSHSEYKEKCVTTLKEALKKDPKLKEPKGLLMVFMLVAKNEINNAF---NKT 131
+ ++ CS + Y + C + + + K+ K ++ + + + + + + K
Sbjct: 65 HHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL 124
Query: 132 ANLKFASKEEKGAYEDCKQLFEDAKEEMGFSITEVGQLDISKLASKEAE-LNNWLSAVIS 190
+K EK A DC + ++ +E+ ++ ++ + K S+ A+ L +SA ++
Sbjct: 125 LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMT 184
Query: 191 YQDTCSDGFPEGELKKKMEMIFAESR----QLLSNSLAVVSQVSQIVNAFQGGLSGFKLP 246
Q TC DGF + K + ++ + ++ SN+LA++ ++
Sbjct: 185 NQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMT---------------- 228
Query: 247 WGKSDAHAPAPDADTDAVADDDEDLADAPDGAPDADQPIFEAPIGAPGAAPIGAPRVDAP 306
DTD + + + D G P G R+
Sbjct: 229 -------------DTDMMIMRTSNNRKLIEETSTVD--------GWPAWLSTGDRRL--- 264
Query: 307 PSWAAPAVDLPGSTEKPTPNVTVAKDGSGDFKTISEALAAIPQTYKGRYVVYVKEGVYDE 366
L S+ P V G +FKT++ ++AA PQ RY++ +K GVY E
Sbjct: 265 ---------LQSSSVTPNVVVAADGSG--NFKTVAASVAAAPQGGTKRYIIRIKAGVYRE 313
Query: 367 TVTVTKKMVNLTMYGDGGLKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAG 426
V VTKK N+ GDG ++IITG++N VDG TF++A+ V+G+GF+ RD+ F+NTAG
Sbjct: 314 NVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITFQNTAG 373
Query: 427 AIKEQAVAARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQ 486
K QAVA RV AD S F NC+ YQDTLY ++RQF+ +C+I+GT+DFIFG+A+AV Q
Sbjct: 374 PSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQ 433
Query: 487 NCQLVLRKPLDNQKNIITANGRIDSKSNTAFVLQKCVIKGEDDL---PSTTKNYIGRPWK 543
NC + RKP QKN++TA GR D NT V+QK I DL + Y+GRPWK
Sbjct: 434 NCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWK 493
Query: 544 EYSRTIIMESDIPALIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIK 603
EYSRT+IM+S I +I P GW W+G+FAL TL+YGE+ N GAGA T RVKW G + I
Sbjct: 494 EYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVIT 553
Query: 604 RG-EALTYTVEPFLDG-SWINGTGVPAHLGL 632
EA +T F+ G SW+ TG P LGL
Sbjct: 554 SATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
>dbj|BAD45460.1| putative pectinesterase [Oryza sativa (japonica cultivar-group)]
Length = 426
Score = 330 bits (847), Expect = 7e-89
Identities = 191/458 (41%), Positives = 258/458 (55%), Gaps = 45/458 (9%)
Query: 185 LSAVISYQDTCSDGF--PEGE-LKKKMEMIFAESRQLLSNSLAVVSQVSQIVNAFQGGLS 241
LSA ++ Q TC DGF +GE ++ ME +++SNSLA+ ++ A GG++
Sbjct: 4 LSAAMTNQYTCLDGFDYKDGERVRHYMESSIHHVSRMVSNSLAM---AKKLPGAGGGGMT 60
Query: 242 GFKLPWGKSDAHAPAPDADTDAVADDDEDLADAPDGAPDADQPIFEAPIGAPGAAPIGAP 301
+ +PD T + ++ + P G G P
Sbjct: 61 ----------PSSSSPDTATQS----------------ESSETTQRQPFMGYGQMANGFP 94
Query: 302 RVDAPPSWAAPAVD--LPGSTEKPTPNVTVAKDGSGDFKTISEALAAIPQTYKGRYVVYV 359
+ W P L TP+ VAKDGSG + T+S A+AA P RYV+++
Sbjct: 95 K------WVRPGDRRLLQAPASSITPDAVVAKDGSGGYTTVSAAVAAAPANSNKRYVIHI 148
Query: 360 KEGVYDETVTVTKKMVNLTMYGDGGLKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDM 419
K G Y E V V K NL GDG K++I ++N VDG TF++A+ V+G+ F+ RD+
Sbjct: 149 KAGAYMENVEVGKSKKNLMFIGDGIGKTVIKASRNVVDGSTTFRSATVAVVGNNFLARDL 208
Query: 420 GFRNTAGAIKEQAVAARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFG 479
N+AG K QAVA RV AD S F C+F GYQDTLY + RQF+R+C I GTIDFIFG
Sbjct: 209 TIENSAGPSKHQAVALRVGADLSAFYRCSFVGYQDTLYVHSLRQFFRECDIYGTIDFIFG 268
Query: 480 HASAVFQNCQLVLRKPLDNQKNIITANGRIDSKSNTAFVLQKCVIKGEDDL---PSTTKN 536
+++ VFQ+C L R+PL NQ N+ TA GR D NT +QKC + DL S+ K
Sbjct: 269 NSAVVFQSCNLYARRPLPNQSNVYTAQGREDPNQNTGISIQKCKVAAASDLLAVQSSFKT 328
Query: 537 YIGRPWKEYSRTIIMESDIPALIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKW 596
Y+GRPWK+YSRT+ M+S++ +++ P GWL W G+FAL TLYYGEY N G GA T RVKW
Sbjct: 329 YLGRPWKQYSRTVFMQSELDSVVNPAGWLEWSGNFALDTLYYGEYQNTGPGASTSNRVKW 388
Query: 597 IGRKDI-KRGEALTYTVEPFLDGS-WINGTGVPAHLGL 632
G + I EA T+TV F+DG W+ GT VP +GL
Sbjct: 389 KGYRVITSASEASTFTVGNFIDGDVWLAGTSVPFTVGL 426
>ref|XP_474065.1| OSJNBb0079B02.7 [Oryza sativa (japonica cultivar-group)]
gi|32490042|emb|CAE05961.1| OSJNBa0063C18.2 [Oryza
sativa (japonica cultivar-group)]
gi|38344904|emb|CAE02974.2| OSJNBb0079B02.7 [Oryza
sativa (japonica cultivar-group)]
Length = 971
Score = 327 bits (839), Expect = 6e-88
Identities = 168/337 (49%), Positives = 218/337 (63%), Gaps = 8/337 (2%)
Query: 301 PRVDAPPSWAAPAVD--LPGSTEKPTPNVTVAKDGSGDFKTISEALAAIPQTYKGRYVVY 358
P+ PSW + L T+KP + VAKDGSGDFKTI+EA+ A+P+ R+V+Y
Sbjct: 634 PKPGEFPSWVSAHQRRLLQAGTQKP--DKVVAKDGSGDFKTITEAVNAVPKNSPTRFVIY 691
Query: 359 VKEGVYDETVTVTKKMVNLTMYGDGGLKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRD 418
VK G Y+E VT+ + N+ MYGDG K+ + GNK+ DGV T T +F G+GFV +
Sbjct: 692 VKAGEYNEYVTIPSSLPNIFMYGDGPTKTRVLGNKSNKDGVATMATRTFSAEGNGFVCKS 751
Query: 419 MGFRNTAGAIKEQAVAARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIF 478
MGF NTAG QAVA VQ D S+F NC FEGYQDTLY +RQF+R+C ++GTID+IF
Sbjct: 752 MGFVNTAGPEGHQAVALHVQGDMSVFFNCKFEGYQDTLYVHANRQFFRNCEVTGTIDYIF 811
Query: 479 GHASAVFQNCQLVLRKPLDNQKNIITANGRIDSKSNTAFVLQKCVIKGEDDL---PSTTK 535
G+++AVFQ+C + +RKP+DNQ N++TA+GR D T VLQ C I E L
Sbjct: 812 GNSAAVFQSCLMTVRKPMDNQANMVTAHGRTDPNMPTGIVLQDCRIVPEQALFPVRLQIA 871
Query: 536 NYIGRPWKEYSRTIIMESDIPALIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVK 595
+Y+GRPWKEY+RT++MES I I+PEGW W GD LKTLYY EY N G GA T RV
Sbjct: 872 SYLGRPWKEYARTVVMESVIGDFIKPEGWSEWMGDVGLKTLYYAEYANTGPGAGTSKRVT 931
Query: 596 WIGRKDIKRGEALTYTVEPFLDG-SWINGTGVPAHLG 631
W G + I + EA +T F+DG +W+ T P +G
Sbjct: 932 WPGYRVIGQAEATQFTAGVFIDGLTWLKNTATPNVMG 968
Score = 82.0 bits (201), Expect = 5e-14
Identities = 58/212 (27%), Positives = 105/212 (49%), Gaps = 29/212 (13%)
Query: 26 LIGVTSVVLIACVIAAVTFVIVKRSGPDHNNNDKKPVQNAPPEPERVDKYSRLVTMLCSH 85
++ VV + C IAAVT S D N V+ ++ +CS
Sbjct: 10 IVAAVGVVAVVCAIAAVT-----SSKKDRNGELTANVR---------------LSTVCSV 49
Query: 86 SEYKEKCVTTLKEALKKDPKLKEPKGLLMVFMLVAKNEINNAFNKTANL--KFASKEEKG 143
+ Y +C +L + +P+ +L + VA E+ +AFN++ ++ +K K
Sbjct: 50 TRYPGRCEQSLGPVVNDTI---DPESVLRAALQVALEEVTSAFNRSMDVGKDDDAKITKS 106
Query: 144 AYEDCKQLFEDAKEEMGFSITEVGQLDISKLASKEAELNNWLSAVISYQDTCSDGFPEGE 203
A E CK+L +DA E++ + L ++ +L WLS+V++Y TC+DGF + E
Sbjct: 107 AIEMCKKLLDDAIEDL----RGMASLKPEEVTKHVNDLRCWLSSVMTYIYTCADGFDKPE 162
Query: 204 LKKKMEMIFAESRQLLSNSLAVVSQVSQIVNA 235
LK+ M+ + S +L SN+LA+++ + +++ A
Sbjct: 163 LKEAMDKLLQNSTELSSNALAIITSLGELMPA 194
>emb|CAC09467.1| putative pectin methylesterase [Oryza sativa (indica
cultivar-group)]
Length = 717
Score = 327 bits (839), Expect = 6e-88
Identities = 168/337 (49%), Positives = 218/337 (63%), Gaps = 8/337 (2%)
Query: 301 PRVDAPPSWAAPAVD--LPGSTEKPTPNVTVAKDGSGDFKTISEALAAIPQTYKGRYVVY 358
P+ PSW + L T+KP + VAKDGSGDFKTI+EA+ A+P+ R+V+Y
Sbjct: 380 PKPGEFPSWVSAHQRRLLQAGTQKP--DKVVAKDGSGDFKTITEAVNAVPKNSPTRFVIY 437
Query: 359 VKEGVYDETVTVTKKMVNLTMYGDGGLKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRD 418
VK G Y+E VT+ + N+ MYGDG K+ + GNK+ DGV T T +F G+GFV +
Sbjct: 438 VKAGEYNEYVTIPSSLPNIFMYGDGPTKTRVLGNKSNKDGVATMATRTFSAEGNGFVCKS 497
Query: 419 MGFRNTAGAIKEQAVAARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIF 478
MGF NTAG QAVA VQ D S+F NC FEGYQDTLY +RQF+R+C ++GTID+IF
Sbjct: 498 MGFVNTAGPEGHQAVALHVQGDMSVFFNCKFEGYQDTLYVHANRQFFRNCEVTGTIDYIF 557
Query: 479 GHASAVFQNCQLVLRKPLDNQKNIITANGRIDSKSNTAFVLQKCVIKGEDDL---PSTTK 535
G+++AVFQ+C + +RKP+DNQ N++TA+GR D T VLQ C I E L
Sbjct: 558 GNSAAVFQSCLMTVRKPMDNQANMVTAHGRTDPNMPTGIVLQDCRIVPEQALFPVRLQIA 617
Query: 536 NYIGRPWKEYSRTIIMESDIPALIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVK 595
+Y+GRPWKEY+RT++MES I I+PEGW W GD LKTLYY EY N G GA T RV
Sbjct: 618 SYLGRPWKEYARTVVMESVIGDFIKPEGWSEWMGDVGLKTLYYAEYANTGPGAGTSKRVT 677
Query: 596 WIGRKDIKRGEALTYTVEPFLDG-SWINGTGVPAHLG 631
W G + I + EA +T F+DG +W+ T P +G
Sbjct: 678 WPGYRVIGQAEATQFTAGVFIDGLTWLKNTATPNVMG 714
Database: nr
Posted date: Jul 5, 2005 12:34 AM
Number of letters in database: 863,360,394
Number of sequences in database: 2,540,612
Lambda K H
0.316 0.134 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,122,025,812
Number of Sequences: 2540612
Number of extensions: 51422220
Number of successful extensions: 172813
Number of sequences better than 10.0: 762
Number of HSP's better than 10.0 without gapping: 334
Number of HSP's successfully gapped in prelim test: 472
Number of HSP's that attempted gapping in prelim test: 170097
Number of HSP's gapped (non-prelim): 1752
length of query: 634
length of database: 863,360,394
effective HSP length: 134
effective length of query: 500
effective length of database: 522,918,386
effective search space: 261459193000
effective search space used: 261459193000
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 78 (34.7 bits)
Medicago: description of AC130802.4