BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148718.4 + phase: 0
(324 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g04360 hypothetical protein 227 6e-60
At4g04940 putative WD-repeat membrane protein 33 0.21
At2g41640 unknown protein 31 1.0
At4g23740 putative receptor kinase 30 1.8
At3g20710 hypothetical protein 30 1.8
At5g45810 CBL-interacting protein kinase 19 (CIPK19) 29 3.0
At2g14420 Mutator-like transposase 29 3.0
At1g74150 hypothetical protein 29 3.0
At1g63190 unknown protein 29 3.0
At3g12570 unknown protein 29 4.0
At5g01540 receptor like protein kinase 28 5.2
At4g18700 putative protein kinase 28 8.8
At3g56140 unknown protein 28 8.8
>At2g04360 hypothetical protein
Length = 295
Score = 227 bits (579), Expect = 6e-60
Identities = 100/157 (63%), Positives = 122/157 (77%)
Query: 168 KSSVPVWPFLSASFFGGIYALLPYFVLWKPPPPPVEEAELKTWPLNFLESKITAMILLAS 227
+S P WPF+ SFFGG+YALLPYF LW PP PPV E EL+ WPLN LESK+TA + L +
Sbjct: 136 ESKTPAWPFVVLSFFGGVYALLPYFALWNPPSPPVSETELRQWPLNVLESKVTAGVTLVA 195
Query: 228 GIGIIIYAGLAGEDVWKEFFQYCRESKFIHITSIDFTLLSTLAPFWVYNDMTARKWFDKG 287
G+GII+Y+ + W EF+QY RESKFIH+TS+DF LLS APFWVYNDMT RKWFDKG
Sbjct: 196 GLGIILYSVVGNAGDWTEFYQYFRESKFIHVTSLDFCLLSAFAPFWVYNDMTTRKWFDKG 255
Query: 288 SWLLPVSLIPLLGPALYILLRPSLSTSAVAQSPAESE 324
SWLLPVS+IP LGP+LY+LLRP++S + + A S+
Sbjct: 256 SWLLPVSVIPFLGPSLYLLLRPAVSETIAPKDTASSD 292
>At4g04940 putative WD-repeat membrane protein
Length = 910
Score = 33.1 bits (74), Expect = 0.21
Identities = 29/108 (26%), Positives = 48/108 (43%), Gaps = 7/108 (6%)
Query: 40 SSFKPLVAMKTPFVAATTSFRRKSSSSSIVICNDSNKQNSSVE-EKEEVKRDWTTSILLF 98
+S K +V +K P V+A TS V+ N Q SS ++++ T S+L
Sbjct: 642 ASGKDVVNVKLPSVSALTSSEADDDMDRQVLENSEALQASSFSISQKQIPELVTLSLLPK 701
Query: 99 LLWAALIYYVSF------LSPNQTPSRDMYFLKKLLNLKGDDGFRMNE 140
W +LI + P + P + +FL + +L GD F+ N+
Sbjct: 702 SQWQSLINLDIIKARNKPIEPPKKPEKAPFFLPSIPSLSGDILFKAND 749
>At2g41640 unknown protein
Length = 500
Score = 30.8 bits (68), Expect = 1.0
Identities = 21/75 (28%), Positives = 33/75 (44%), Gaps = 9/75 (12%)
Query: 24 IQPHITVVASG--CCCRLSSFKPLVAMKTPFVAATTSFRRKSSSSSIVICNDSNKQNSSV 81
I P + +++G CC R + MK R S+SSSI + S N+
Sbjct: 87 ITPLCSEISNGTICCDRTGLRSDICVMKG-------DVRTNSASSSIFLFTSSTNNNTKP 139
Query: 82 EEKEEVKRDWTTSIL 96
E+ + R W TS++
Sbjct: 140 EKIKPYTRKWETSVM 154
>At4g23740 putative receptor kinase
Length = 638
Score = 30.0 bits (66), Expect = 1.8
Identities = 17/56 (30%), Positives = 24/56 (42%), Gaps = 7/56 (12%)
Query: 183 GGIYALLPYFVLWKPPPPPVEEAELKTWPLNFLESKITAMILLASGIGIIIYAGLA 238
GG Y L+ PPPP E+ K FL T +L+ + I++ LA
Sbjct: 226 GGNYTLVT-------PPPPSEQTHQKPSKARFLGLSETVFLLIVIAVSIVVITALA 274
>At3g20710 hypothetical protein
Length = 362
Score = 30.0 bits (66), Expect = 1.8
Identities = 13/43 (30%), Positives = 26/43 (60%)
Query: 109 SFLSPNQTPSRDMYFLKKLLNLKGDDGFRMNEVLVSEWYIMGF 151
S++ P+ T RDM ++K+ ++L GD + + +WY++ F
Sbjct: 179 SWMIPDGTLERDMEYMKQGVSLNGDTYWYAKDKESIDWYLLCF 221
>At5g45810 CBL-interacting protein kinase 19 (CIPK19)
Length = 483
Score = 29.3 bits (64), Expect = 3.0
Identities = 18/75 (24%), Positives = 30/75 (40%), Gaps = 14/75 (18%)
Query: 91 WTTSILLFLLWAAL--------------IYYVSFLSPNQTPSRDMYFLKKLLNLKGDDGF 136
W+ ++LF+L A IY F P P L ++L K + F
Sbjct: 212 WSCGVILFVLMAGFLPFHDRNVMAMYKKIYRGDFRCPRWFPVEINRLLIRMLETKPERRF 271
Query: 137 RMNEVLVSEWYIMGF 151
M +++ + W+ GF
Sbjct: 272 TMPDIMETSWFKKGF 286
>At2g14420 Mutator-like transposase
Length = 241
Score = 29.3 bits (64), Expect = 3.0
Identities = 11/26 (42%), Positives = 16/26 (61%)
Query: 137 RMNEVLVSEWYIMGFWPLVYSMLLLP 162
++ E +VS+WY + W L YS L P
Sbjct: 108 KLPETVVSDWYSIEKWKLCYSSLFFP 133
>At1g74150 hypothetical protein
Length = 552
Score = 29.3 bits (64), Expect = 3.0
Identities = 16/52 (30%), Positives = 29/52 (55%), Gaps = 4/52 (7%)
Query: 135 GFRMNEVLVSEWYIM----GFWPLVYSMLLLPTGRSSKSSVPVWPFLSASFF 182
GF ++ L + Y++ G W V +M+ P+ R S ++V + P+ + SFF
Sbjct: 249 GFTDSQNLYDDLYVLDLETGVWSKVVAMVEGPSARFSSAAVCLDPYKAGSFF 300
>At1g63190 unknown protein
Length = 217
Score = 29.3 bits (64), Expect = 3.0
Identities = 13/32 (40%), Positives = 16/32 (49%)
Query: 179 ASFFGGIYALLPYFVLWKPPPPPVEEAELKTW 210
+ F G I L P F+LWKP P E + W
Sbjct: 2 SEFEGWIKWLEPVFLLWKPEDPGYERPAYRDW 33
>At3g12570 unknown protein
Length = 489
Score = 28.9 bits (63), Expect = 4.0
Identities = 16/52 (30%), Positives = 26/52 (49%)
Query: 33 SGCCCRLSSFKPLVAMKTPFVAATTSFRRKSSSSSIVICNDSNKQNSSVEEK 84
+G C LS+ P A+ T V S +RK ++ C+DS+ S++ K
Sbjct: 287 NGTCLNLSTHSPDHAVDTVEVQLAGSNKRKRDCLALGNCDDSSSSEKSLDMK 338
>At5g01540 receptor like protein kinase
Length = 682
Score = 28.5 bits (62), Expect = 5.2
Identities = 20/70 (28%), Positives = 34/70 (48%), Gaps = 9/70 (12%)
Query: 164 GRSSKSSVPVWPFLSASFFGGIYALLPYFVLWKPPPPPVEEAELKTWPLNFLESKITAMI 223
G+SS V W F S GG + +L + PPPP +A+ + L S++ MI
Sbjct: 264 GQSSAHYVMGWSFSS----GGERPIADVLILSELPPPPPNKAKKEG-----LNSQVIVMI 314
Query: 224 LLASGIGIII 233
+ S + +++
Sbjct: 315 VALSAVMLVM 324
>At4g18700 putative protein kinase
Length = 489
Score = 27.7 bits (60), Expect = 8.8
Identities = 18/75 (24%), Positives = 28/75 (37%), Gaps = 14/75 (18%)
Query: 91 WTTSILLFLLWAAL--------------IYYVSFLSPNQTPSRDMYFLKKLLNLKGDDGF 136
W+ ++LF+L A IY F P + L KLL + F
Sbjct: 210 WSCGVILFVLMAGYLPFHDRNVMAMYKKIYRGEFRCPRWFSTELTRLLSKLLETNPEKRF 269
Query: 137 RMNEVLVSEWYIMGF 151
E++ + W+ GF
Sbjct: 270 TFPEIMENSWFKKGF 284
>At3g56140 unknown protein
Length = 745
Score = 27.7 bits (60), Expect = 8.8
Identities = 20/54 (37%), Positives = 30/54 (55%), Gaps = 9/54 (16%)
Query: 276 NDMTARKWFDKGSWLLPVSLIPLLGPALYILLRPSL------STSAVAQSPAES 323
+D+ +R F + ++LL PLL A + L+PS+ S+SA SPAES
Sbjct: 55 SDLPSRTAFSRRAFLLAP---PLLVSAASLFLKPSVSLASEESSSATVTSPAES 105
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.322 0.137 0.428
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,570,851
Number of Sequences: 26719
Number of extensions: 326521
Number of successful extensions: 1176
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1168
Number of HSP's gapped (non-prelim): 15
length of query: 324
length of database: 11,318,596
effective HSP length: 99
effective length of query: 225
effective length of database: 8,673,415
effective search space: 1951518375
effective search space used: 1951518375
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)
Medicago: description of AC148718.4