BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146330.2 + phase: 0
(223 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g60820 proteasome component C5 400 e-112
At1g21720 20S proteasome beta subunit PBC2 like protein 78 3e-15
At1g77440 putative 20S proteasome beta subunit PBC2 75 2e-14
At4g31300 multicatalytic endopeptidase complex, proteasome precu... 65 4e-11
At3g26340 26S proteasome beta subunit like protein 44 9e-05
At1g13060 proteasome epsilon chain precursor 42 2e-04
At4g14800 proteasome chain protein 40 7e-04
At3g22630 multicatalytic endopeptidase complex, proteasome compo... 38 0.004
At1g56450 putative protein (At1g56450) 34 0.054
At5g61130 predicted GPI-anchored protein 28 5.0
At4g17260 lactate dehydrogenase (LDH1) 28 5.0
At5g48520 unknown protein 27 6.6
At3g06140 putative RING zinc finger protein 27 6.6
At5g59480 putative ripening-related protein - like 27 8.6
At4g34910 RNA helicase (RH16) 27 8.6
At4g26920 putative homeodomain protein 27 8.6
At1g21700 putative transcriptional regulatory protein 27 8.6
>At3g60820 proteasome component C5
Length = 223
Score = 400 bits (1029), Expect = e-112
Identities = 195/223 (87%), Positives = 209/223 (93%)
Query: 1 MTKQHANWSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMAS 60
MTKQHANWSPYDNNGG+CVAIAGSDYCVIAADTRMSTGY+IL+RDYSKI LA++ V++S
Sbjct: 1 MTKQHANWSPYDNNGGTCVAIAGSDYCVIAADTRMSTGYSILSRDYSKIHKLADRAVLSS 60
Query: 61 SGFQADVKALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLD 120
SGFQADVKALQKVL +RHL YQHQHNKQMSCPAMAQLLSNTLY+KRFFPYY+FNVLGGLD
Sbjct: 61 SGFQADVKALQKVLKSRHLIYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLD 120
Query: 121 SEGKGCVFTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAE 180
EGKGCVFTYDAVGSYERVGY +QGSGSTLIMPFLDNQLKSPSPLLLP QD+ TPLSEAE
Sbjct: 121 EEGKGCVFTYDAVGSYERVGYGAQGSGSTLIMPFLDNQLKSPSPLLLPKQDSNTPLSEAE 180
Query: 181 AVDLVKTVFASATERDIYTGDKVEIVILNAGGIHREFMDLRKD 223
AVDLVKTVFASATERDIYTGDK+EI+IL A GI E MDLRKD
Sbjct: 181 AVDLVKTVFASATERDIYTGDKLEIMILKADGIKTELMDLRKD 223
>At1g21720 20S proteasome beta subunit PBC2 like protein
Length = 204
Score = 78.2 bits (191), Expect = 3e-15
Identities = 59/216 (27%), Positives = 96/216 (44%), Gaps = 14/216 (6%)
Query: 9 SPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVK 68
S ++ NG + VA+ G + IA+D R+ + D+ +IS + ++ + SG DV+
Sbjct: 2 SIFEYNGSAVVAMVGKNCFAIASDRRLGVQLQTIATDFQRISKIHDRVFIGLSGLATDVQ 61
Query: 69 ALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCVF 128
L + L RH YQ + + M A L+S LY KRF PY V+ GL + K +
Sbjct: 62 TLYQRLVFRHKLYQLREERDMKPETFASLVSAILYEKRFGPYLCQPVIAGLGDDDKPFIC 121
Query: 129 TYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAV-DLVKT 187
T D++G+ E L F+ + S S L + P EAE + + +
Sbjct: 122 TMDSIGAKE------------LAKDFVVSGTASES-LYGACEAMYKPDMEAEELFETISQ 168
Query: 188 VFASATERDIYTGDKVEIVILNAGGIHREFMDLRKD 223
S+ +RD +G + I+ I + R D
Sbjct: 169 ALLSSVDRDCLSGWGGHVYIVTPTEIKERILKGRMD 204
>At1g77440 putative 20S proteasome beta subunit PBC2
Length = 204
Score = 75.5 bits (184), Expect = 2e-14
Identities = 41/129 (31%), Positives = 66/129 (50%)
Query: 9 SPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVK 68
S ++ NG + VA+ G + IA+D R+ + D+ +IS + + + SG DV+
Sbjct: 2 SIFEYNGSAVVAMVGKNCFAIASDRRLGVQLQTIATDFQRISKIHDHLFIGLSGLATDVQ 61
Query: 69 ALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCVF 128
L + L RH YQ + + M A L+S LY KRF P+ V+ GL + K +
Sbjct: 62 TLYQRLVFRHKLYQLREERDMKPETFASLVSAILYEKRFGPFLCQPVIAGLGDDNKPFIC 121
Query: 129 TYDAVGSYE 137
T D++G+ E
Sbjct: 122 TMDSIGAKE 130
>At4g31300 multicatalytic endopeptidase complex, proteasome
precursor, beta subunit
Length = 233
Score = 64.7 bits (156), Expect = 4e-11
Identities = 56/209 (26%), Positives = 90/209 (42%), Gaps = 26/209 (12%)
Query: 15 GGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKALQKVL 74
G + + + + V+ AD+R STG + R KI+ L + + SG AD +V+
Sbjct: 12 GTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAAD----SQVV 67
Query: 75 SARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVL------GGLDSEGKGCVF 128
S + HQH Q PA ++ +N + R Y + N+L GG D G ++
Sbjct: 68 SDYVRYFLHQHTIQHGQPATVKVSANLI---RMLAYNNKNMLQTGLIVGGWDKYEGGKIY 124
Query: 129 TYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKTV 188
G+ ++ GSGS+ + F D K +++ EA LV
Sbjct: 125 GIPLGGTVVEQPFAIGGSGSSYLYGFFDQAWKD-------------NMTKEEAEQLVVKA 171
Query: 189 FASATERDIYTGDKVEIVILNAGGIHREF 217
+ A RD +G V VI+N+ G+ R F
Sbjct: 172 VSLAIARDGASGGVVRTVIINSEGVTRNF 200
>At3g26340 26S proteasome beta subunit like protein
Length = 273
Score = 43.5 bits (101), Expect = 9e-05
Identities = 45/186 (24%), Positives = 78/186 (41%), Gaps = 14/186 (7%)
Query: 15 GGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKALQKVL 74
G + +A + ++AAD+R S G I ++ KI + + +G AD + + L
Sbjct: 57 GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNL 116
Query: 75 SARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCVFTYDAVG 134
+ ++ + +++S ++LL+N LY R ++ G D G G ++ D G
Sbjct: 117 GIKCRLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPG-LYYVDNEG 175
Query: 135 SYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKTVFASATE 194
+ S GSGS LD+ K +S EA +L + AT
Sbjct: 176 GRLKGDRFSVGSGSPYAYGVLDSGYK-------------FDMSVEEASELARRSIYHATF 222
Query: 195 RDIYTG 200
RD +G
Sbjct: 223 RDGASG 228
>At1g13060 proteasome epsilon chain precursor
Length = 274
Score = 42.4 bits (98), Expect = 2e-04
Identities = 43/173 (24%), Positives = 73/173 (41%), Gaps = 14/173 (8%)
Query: 28 VIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKALQKVLSARHLTYQHQHNK 87
++AAD+R S G I ++ KI + + +G AD + + L + ++ + +
Sbjct: 70 MVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKR 129
Query: 88 QMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCVFTYDAVGSYERVGYSSQGSG 147
++S ++LL+N LY R ++ G D G G ++ D G + S GSG
Sbjct: 130 RISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPG-LYYVDNEGGRLKGDRFSVGSG 188
Query: 148 STLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKTVFASATERDIYTG 200
S LD+ K +S EA +L + AT RD +G
Sbjct: 189 SPYAYGVLDSGYK-------------YDMSVEEASELARRSIYHATFRDGASG 228
>At4g14800 proteasome chain protein
Length = 199
Score = 40.4 bits (93), Expect = 7e-04
Identities = 43/197 (21%), Positives = 73/197 (36%), Gaps = 15/197 (7%)
Query: 18 CV-AIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKALQKVLSA 76
CV + G+ + ++AADT + KI L ++A+SG D + +
Sbjct: 3 CVFGLVGNGFAIVAADTSAVHSILLHKNKEDKIMTLDSHKLVAASGEPGDRVQFTEYVQK 62
Query: 77 RHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVL-GGLDSEGKGCVFTYDAVGS 135
YQ ++ +S A A L YS N+L G D E ++ D + +
Sbjct: 63 NVSLYQFRNGIPLSTAAAANFTRGELATALRKNPYSVNILMAGYDKEAGASLYYIDYIAT 122
Query: 136 YERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKTVFASATER 195
+V + G GS + +D +S +S EA++LV R
Sbjct: 123 LHKVDKGAFGYGSYFSLSTMDRHYRS-------------DMSVEEAIELVDKCILEIRSR 169
Query: 196 DIYTGDKVEIVILNAGG 212
+ I I++ G
Sbjct: 170 LVIAPPNFVIKIVDKDG 186
>At3g22630 multicatalytic endopeptidase complex, proteasome
component, beta subunit
Length = 204
Score = 38.1 bits (87), Expect = 0.004
Identities = 41/197 (20%), Positives = 74/197 (36%), Gaps = 15/197 (7%)
Query: 18 CV-AIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKALQKVLSA 76
CV + G+ + ++AADT + + KI L ++A+SG D + +
Sbjct: 3 CVFGLVGNGFAIVAADTSAVHSILLHKNNEDKIMVLDSHKLVAASGEPGDRVQFTEYVQK 62
Query: 77 RHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVL-GGLDSEGKGCVFTYDAVGS 135
Y+ ++ ++ A A L YS N+L G D E ++ D + +
Sbjct: 63 NVSLYKFRNGIPLTTAAAANFTRGELATALRKNPYSVNILMAGYDDESGASLYYIDYIAT 122
Query: 136 YERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKTVFASATER 195
+V + G GS + +D +S +S EA++LV R
Sbjct: 123 LHKVDKGAFGYGSYFSLSTMDRHYRS-------------DMSVEEAIELVDKCILEIRSR 169
Query: 196 DIYTGDKVEIVILNAGG 212
+ I I++ G
Sbjct: 170 LVVAPPNFVIKIVDKDG 186
>At1g56450 putative protein (At1g56450)
Length = 246
Score = 34.3 bits (77), Expect = 0.054
Identities = 32/147 (21%), Positives = 64/147 (42%), Gaps = 4/147 (2%)
Query: 15 GGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKALQKVL 74
G S VAI D ++A+D S G + ++ ++ + + ++ +SG +D + + + L
Sbjct: 31 GTSIVAIKYKDGVLMASDMGGSYGSTLRYKNIERVKAIGKHSLLGASGEISDFQEILRYL 90
Query: 75 SARHLT-YQHQHNKQMSCPAMAQLLSNTLYYKR--FFPYYSFNVLGGLDSEGKGCVFTYD 131
L + + L+ +Y +R F P ++ VLGG+ GK +
Sbjct: 91 DELTLNDNMWDDGNSLGPKEIHNYLTRVMYNRRNKFNPLWNTLVLGGV-KNGKSYLGMVS 149
Query: 132 AVGSYERVGYSSQGSGSTLIMPFLDNQ 158
+G + + G G+ L P L ++
Sbjct: 150 MIGVSFEDDHVATGFGNHLARPILRDE 176
>At5g61130 predicted GPI-anchored protein
Length = 201
Score = 27.7 bits (60), Expect = 5.0
Identities = 19/58 (32%), Positives = 22/58 (37%), Gaps = 10/58 (17%)
Query: 120 DSEGKGCVFTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLS 177
D GC F A GS SGST + P N SP+ LP +P S
Sbjct: 96 DPSYTGCAFPTSASGS----------SGSTTVTPGTTNPKGSPTTTTLPGSGTNSPYS 143
>At4g17260 lactate dehydrogenase (LDH1)
Length = 353
Score = 27.7 bits (60), Expect = 5.0
Identities = 14/42 (33%), Positives = 21/42 (49%), Gaps = 3/42 (7%)
Query: 22 AGSDYCVIAADTRMSTG---YNILTRDYSKISHLAEKCVMAS 60
AGSD C++ A R + G N+L R+ + H+ AS
Sbjct: 107 AGSDLCIVTAGARQNPGESRLNLLQRNVALFRHIIPPLAKAS 148
>At5g48520 unknown protein
Length = 617
Score = 27.3 bits (59), Expect = 6.6
Identities = 15/46 (32%), Positives = 24/46 (51%)
Query: 34 RMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKALQKVLSARHL 79
R+ T Y++LT S + V A+S + A++ LSAR+L
Sbjct: 125 RLQTQYDLLTGQSSALIQGRRARVAATSAVSGQITAIEDSLSARNL 170
>At3g06140 putative RING zinc finger protein
Length = 546
Score = 27.3 bits (59), Expect = 6.6
Identities = 24/82 (29%), Positives = 40/82 (48%), Gaps = 6/82 (7%)
Query: 145 GSGSTLIMPFLDNQLKSPS----PLLLPAQDAVTPLSEAEAVDLVKTVFASATERDIYTG 200
G+G+ L LD+ K PL++ A+ ++P S +E + K V + E+D
Sbjct: 387 GTGTDLSFFVLDDLSKPLEEDVYPLVISAETIISPNSISEQSSVHKQVTQAVLEKDNDGS 446
Query: 201 DKVEIV--ILNAGGIHREFMDL 220
KV++V IL G+ E +L
Sbjct: 447 FKVKVVKQILWIEGVRYELREL 468
>At5g59480 putative ripening-related protein - like
Length = 282
Score = 26.9 bits (58), Expect = 8.6
Identities = 16/59 (27%), Positives = 30/59 (50%)
Query: 158 QLKSPSPLLLPAQDAVTPLSEAEAVDLVKTVFASATERDIYTGDKVEIVILNAGGIHRE 216
+L S + P++ A + + ++ KT+F + R+I TG +V + + G HRE
Sbjct: 181 ELPKTSVVCKPSEGAFEQVFKMANINPKKTLFFDDSIRNIQTGKRVGLHTVWVGTSHRE 239
>At4g34910 RNA helicase (RH16)
Length = 626
Score = 26.9 bits (58), Expect = 8.6
Identities = 18/68 (26%), Positives = 35/68 (51%), Gaps = 2/68 (2%)
Query: 31 ADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKALQKVL--SARHLTYQHQHNKQ 88
AD +S GY R + I +C++ S+ +DV+ L+K++ + LT ++K+
Sbjct: 209 ADLLLSYGYEDNLRSVTSIIPRRCQCLLMSATTSSDVEKLKKLILHNPIVLTLTEDNDKE 268
Query: 89 MSCPAMAQ 96
+ P+ Q
Sbjct: 269 EAVPSNVQ 276
>At4g26920 putative homeodomain protein
Length = 461
Score = 26.9 bits (58), Expect = 8.6
Identities = 10/20 (50%), Positives = 15/20 (75%)
Query: 96 QLLSNTLYYKRFFPYYSFNV 115
++L+ YY RFFP+Y+ NV
Sbjct: 93 EMLTLNEYYSRFFPWYAKNV 112
>At1g21700 putative transcriptional regulatory protein
Length = 807
Score = 26.9 bits (58), Expect = 8.6
Identities = 12/27 (44%), Positives = 19/27 (69%), Gaps = 4/27 (14%)
Query: 140 GYSSQGSGSTLIMPFLDNQLKSPSPLL 166
GYS QGS + + +PF +KSP+P++
Sbjct: 482 GYSEQGSDTEIKLPF----VKSPNPVM 504
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.318 0.133 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,821,229
Number of Sequences: 26719
Number of extensions: 185448
Number of successful extensions: 432
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 418
Number of HSP's gapped (non-prelim): 18
length of query: 223
length of database: 11,318,596
effective HSP length: 96
effective length of query: 127
effective length of database: 8,753,572
effective search space: 1111703644
effective search space used: 1111703644
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC146330.2