BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC131249.9 - phase: 0 /pseudo
(421 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g58050 putative protein 37 0.027
At5g53800 unknown protein 36 0.046
At2g29210 proline-rich protein like 35 0.059
At5g60530 late embryonic abundant protein - like 32 0.50
At3g07280 unknown protein 32 0.50
At2g41960 unknown protein 32 0.66
At3g16840 ATP-dependent RNA helicase 32 0.86
At4g35940 putative protein 31 1.1
At1g20920 putative RNA helicase 31 1.1
At2g39320 hypothetical protein 31 1.5
At1g65280 31 1.5
At1g60900 putative U2 snRNP auxiliary factor 30 2.5
At3g57150 putative pseudouridine synthase (NAP57) 30 3.3
At3g52220 unknown protein 29 4.3
At2g18540 putative vicilin storage protein (globulin-like) 29 4.3
At1g56660 hypothetical protein 29 4.3
At1g44910 splicing factor like protein 29 4.3
At5g67320 unknown protein 29 5.6
At5g64200 unknown protein 29 5.6
At3g05670 unknown protein 29 5.6
>At3g58050 putative protein
Length = 1209
Score = 36.6 bits (83), Expect = 0.027
Identities = 15/42 (35%), Positives = 28/42 (65%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKKC 274
K + +E ERK R ++R+K+ R ++R + K GK+++N +C
Sbjct: 537 KEKREEEERKEKKRSKEREKKLRKKERLKEKDKGKEKKNPEC 578
>At5g53800 unknown protein
Length = 339
Score = 35.8 bits (81), Expect = 0.046
Identities = 16/41 (39%), Positives = 26/41 (63%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
KR+ KE E + RKR+R+++ + ++ K K KKR+ KK
Sbjct: 134 KRKRKEREEEEKERKRRRREKDKKKRNKSDKDGDKKRKEKK 174
Score = 33.5 bits (75), Expect = 0.23
Identities = 18/41 (43%), Positives = 27/41 (64%), Gaps = 4/41 (9%)
Query: 236 SKENERKICARKRKRKKRKRNRKRKERKFH----GKKRRNK 272
S+E+E + R+RKRK+++R + KERK KK+RNK
Sbjct: 121 SEESESEDERRRRKRKRKEREEEEKERKRRRREKDKKKRNK 161
Score = 29.3 bits (64), Expect = 4.3
Identities = 13/41 (31%), Positives = 25/41 (60%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
+R +E ERK R++ +KKR ++ K ++K KK++ +
Sbjct: 139 EREEEEKERKRRRREKDKKKRNKSDKDGDKKRKEKKKKKSE 179
Score = 28.9 bits (63), Expect = 5.6
Identities = 12/35 (34%), Positives = 21/35 (59%)
Query: 238 ENERKICARKRKRKKRKRNRKRKERKFHGKKRRNK 272
++E +R+R+KRKR + +E K ++RR K
Sbjct: 120 DSEESESEDERRRRKRKRKEREEEEKERKRRRREK 154
>At2g29210 proline-rich protein like
Length = 878
Score = 35.4 bits (80), Expect = 0.059
Identities = 19/41 (46%), Positives = 30/41 (72%), Gaps = 3/41 (7%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
KRR KE E+K ++KR++ +R+RKR+ER+ G K ++KK
Sbjct: 793 KRRRKE-EKKTRKEEKKRRREERHRKREERR--GGKEKHKK 830
>At5g60530 late embryonic abundant protein - like
Length = 439
Score = 32.3 bits (72), Expect = 0.50
Identities = 14/41 (34%), Positives = 28/41 (68%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
++ K+ ERK +K K +K K++++RKE++ K+R+ K+
Sbjct: 85 EKEKKDKERKEKEKKDKLEKEKKDKERKEKERKEKERKAKE 125
Score = 31.6 bits (70), Expect = 0.86
Identities = 12/40 (30%), Positives = 26/40 (65%)
Query: 234 RRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
+ KE E+K K K+ K ++ ++RKE++ K++++K+
Sbjct: 91 KERKEKEKKDKLEKEKKDKERKEKERKEKERKAKEKKDKE 130
Score = 31.6 bits (70), Expect = 0.86
Identities = 14/41 (34%), Positives = 26/41 (63%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
K+ +E E+K RK K KK K +++K+++ K+R+ K+
Sbjct: 80 KKDKEEKEKKDKERKEKEKKDKLEKEKKDKERKEKERKEKE 120
Score = 28.1 bits (61), Expect = 9.5
Identities = 12/39 (30%), Positives = 23/39 (58%)
Query: 235 RSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
+ KE E+K + K KK K + ++E++ K+R+ K+
Sbjct: 59 KDKEQEKKDKEKAAKDKKEKEKKDKEEKEKKDKERKEKE 97
>At3g07280 unknown protein
Length = 481
Score = 32.3 bits (72), Expect = 0.50
Identities = 16/41 (39%), Positives = 28/41 (68%), Gaps = 1/41 (2%)
Query: 233 KRRSKENERKICARKRKRKKRK-RNRKRKERKFHGKKRRNK 272
K+ K+ E+K KR+++K K ++++RKERK K R++K
Sbjct: 32 KKHKKDKEKKEGKEKREKEKSKDKHKERKERKEKHKDRKDK 72
>At2g41960 unknown protein
Length = 1215
Score = 32.0 bits (71), Expect = 0.66
Identities = 17/57 (29%), Positives = 31/57 (53%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKKCHCHQAAPIFAILQRG 289
K + +E ERK R ++R+K+ R ++R + K K+++N K PI + + G
Sbjct: 506 KEKREEEERKERKRIKEREKKLRRKERLKEKEREKEQKNPKFSDKAILPIMSREEEG 562
>At3g16840 ATP-dependent RNA helicase
Length = 832
Score = 31.6 bits (70), Expect = 0.86
Identities = 16/40 (40%), Positives = 26/40 (65%), Gaps = 3/40 (7%)
Query: 234 RRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
R +++E RK+K KK KRN+++K+ K KK++ KK
Sbjct: 119 RDDEDDEDDEETRKKKEKKAKRNKEKKKEK---KKKKQKK 155
>At4g35940 putative protein
Length = 451
Score = 31.2 bits (69), Expect = 1.1
Identities = 18/42 (42%), Positives = 28/42 (65%), Gaps = 3/42 (7%)
Query: 234 RRSKENERKICARKRKRKKRKRNRKRKER--KFHGKKRRNKK 273
RRS + ++K ++RK KK K+ +KRKER K G ++R+ K
Sbjct: 43 RRSDKKDKKD-KKERKEKKEKKEKKRKEREGKEVGSEKRSHK 83
Score = 28.5 bits (62), Expect = 7.3
Identities = 10/41 (24%), Positives = 28/41 (67%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
+R+ + +++K K++RK++K +++K ++ GK+ ++K
Sbjct: 39 RRKDRRSDKKDKKDKKERKEKKEKKEKKRKEREGKEVGSEK 79
>At1g20920 putative RNA helicase
Length = 1166
Score = 31.2 bits (69), Expect = 1.1
Identities = 13/41 (31%), Positives = 26/41 (62%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
+ R K+ ER+ R+ + K+R + R+R+ER+ + RR ++
Sbjct: 148 REREKDRERREREREEREKERVKERERREREDGERDRRERE 188
Score = 30.8 bits (68), Expect = 1.5
Identities = 14/41 (34%), Positives = 25/41 (60%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
KR + ERK R+R++ + +R R+R+ER+ K R ++
Sbjct: 135 KRDREREERKDKEREREKDRERREREREEREKERVKERERR 175
Score = 28.9 bits (63), Expect = 5.6
Identities = 15/44 (34%), Positives = 27/44 (61%), Gaps = 3/44 (6%)
Query: 233 KRRSKENERKICARKRKRKKRKR---NRKRKERKFHGKKRRNKK 273
+RR +E E + R ++R++R+R R R+ER+ RRN++
Sbjct: 155 ERREREREEREKERVKERERREREDGERDRREREKERGSRRNRE 198
Score = 28.1 bits (61), Expect = 9.5
Identities = 13/41 (31%), Positives = 27/41 (65%), Gaps = 2/41 (4%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
K R ++ ER+ RK K ++R+++R+R+ER+ +++ K
Sbjct: 132 KDRKRDRERE--ERKDKEREREKDRERREREREEREKERVK 170
>At2g39320 hypothetical protein
Length = 189
Score = 30.8 bits (68), Expect = 1.5
Identities = 14/41 (34%), Positives = 24/41 (58%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
K+ +E ERK ++ K+K ++ +K KE K K ++ KK
Sbjct: 133 KKEEEEKERKDMEKEEKKKDKEDKKKDKEDKKKAKVQKEKK 173
Score = 29.3 bits (64), Expect = 4.3
Identities = 14/45 (31%), Positives = 25/45 (55%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKKCHCH 277
K KE ++K K+K K+ K+ K ++ K K+++N+ H H
Sbjct: 142 KDMEKEEKKKDKEDKKKDKEDKKKAKVQKEKKEKKEKKNRNHHFH 186
>At1g65280
Length = 605
Score = 30.8 bits (68), Expect = 1.5
Identities = 15/35 (42%), Positives = 24/35 (67%), Gaps = 3/35 (8%)
Query: 233 KRRSKENER---KICARKRKRKKRKRNRKRKERKF 264
KRR +E ER KI ++RKR+ K+ +K K+R++
Sbjct: 57 KRRKREKERKRKKIERKERKRRDMKKKKKTKKREY 91
Score = 30.0 bits (66), Expect = 2.5
Identities = 11/40 (27%), Positives = 23/40 (57%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNK 272
+++ KE + + K+RKR ++RK +K K+R+ +
Sbjct: 39 RKKQKERRKSDGGSYEREKRRKREKERKRKKIERKERKRR 78
Score = 29.6 bits (65), Expect = 3.3
Identities = 14/37 (37%), Positives = 25/37 (66%), Gaps = 3/37 (8%)
Query: 237 KENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
+E RK R+++RK++K RK ++R+ KK++ KK
Sbjct: 55 REKRRK---REKERKRKKIERKERKRRDMKKKKKTKK 88
Score = 28.1 bits (61), Expect = 9.5
Identities = 15/41 (36%), Positives = 25/41 (60%), Gaps = 2/41 (4%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
K R K + KR++++++R RK+ ERK +KRR+ K
Sbjct: 43 KERRKSDGGSYEREKRRKREKERKRKKIERK--ERKRRDMK 81
>At1g60900 putative U2 snRNP auxiliary factor
Length = 589
Score = 30.0 bits (66), Expect = 2.5
Identities = 11/40 (27%), Positives = 24/40 (59%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNK 272
+RRS++ +R++ R+R R + + +R+ R H K ++
Sbjct: 135 RRRSRDRDREVRHRRRSRSRSRSRSERRSRSEHRHKSEHR 174
>At3g57150 putative pseudouridine synthase (NAP57)
Length = 565
Score = 29.6 bits (65), Expect = 3.3
Identities = 13/37 (35%), Positives = 22/37 (59%)
Query: 237 KENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
+E E K+ + K+K+KK K K +E K+++ KK
Sbjct: 467 EEAEEKVKSSKKKKKKDKEEEKEEEAGSEKKEKKKKK 503
>At3g52220 unknown protein
Length = 237
Score = 29.3 bits (64), Expect = 4.3
Identities = 16/51 (31%), Positives = 30/51 (58%), Gaps = 10/51 (19%)
Query: 233 KRRSKENERKICARKR-----KRKKRKRNRKRK-----ERKFHGKKRRNKK 273
+++ + ERK R++ +R+KR+R+ KR +RK H K+++ KK
Sbjct: 179 QKKDRREERKPAKREKEERHDRREKRERHEKRSARDSDDRKKHKKEKKEKK 229
>At2g18540 putative vicilin storage protein (globulin-like)
Length = 699
Score = 29.3 bits (64), Expect = 4.3
Identities = 13/41 (31%), Positives = 25/41 (60%), Gaps = 2/41 (4%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
K+R +E +RK R+ +KR+ ++RK R+ +KR ++
Sbjct: 523 KKREEERQRK--EREEVERKRREEQERKRREEEARKREEER 561
Score = 28.9 bits (63), Expect = 5.6
Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 9/50 (18%)
Query: 233 KRRSKENERKICARKRKR---------KKRKRNRKRKERKFHGKKRRNKK 273
+ R K E ARKR+ KKR+ R+RKER+ +KRR ++
Sbjct: 496 EERKKREEEAEQARKREEEREKEEEMAKKREEERQRKEREEVERKRREEQ 545
>At1g56660 hypothetical protein
Length = 522
Score = 29.3 bits (64), Expect = 4.3
Identities = 14/47 (29%), Positives = 27/47 (56%), Gaps = 7/47 (14%)
Query: 234 RRSKENERKICARKRKRKKRKRNRKRKE-------RKFHGKKRRNKK 273
+++K+ E+ + K+KK K+ +K+KE +K GKK + +K
Sbjct: 175 KKNKKKEKDESGTEEKKKKPKKEKKQKEESKSNEDKKVKGKKEKGEK 221
Score = 28.1 bits (61), Expect = 9.5
Identities = 13/47 (27%), Positives = 26/47 (54%), Gaps = 6/47 (12%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKE------RKFHGKKRRNKK 273
K+ K+ + + CA ++K+K K +++ E +K GKK + +K
Sbjct: 248 KKNKKKEKDESCAEEKKKKPDKEKKEKDESTEKEDKKLKGKKGKGEK 294
>At1g44910 splicing factor like protein
Length = 958
Score = 29.3 bits (64), Expect = 4.3
Identities = 15/40 (37%), Positives = 23/40 (57%), Gaps = 3/40 (7%)
Query: 234 RRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
R+ KE + K KRK K ++R K +ER+ K R+K+
Sbjct: 819 RKEKERDEK---EKRKDKDKERREKEREREKEKGKERSKR 855
Score = 28.5 bits (62), Expect = 7.3
Identities = 12/29 (41%), Positives = 20/29 (68%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKE 261
KR+ K+ ER+ R+R+++K K KR+E
Sbjct: 829 KRKDKDKERREKEREREKEKGKERSKREE 857
Score = 28.1 bits (61), Expect = 9.5
Identities = 17/42 (40%), Positives = 25/42 (59%), Gaps = 4/42 (9%)
Query: 233 KRRSKENERKICARK-RKRKKRKRNRKRKERKFHGKKRRNKK 273
+ ++KE ERK K RK K+R KRK++ K+RR K+
Sbjct: 803 QEKAKEKERKRDEEKVRKEKERDEKEKRKDK---DKERREKE 841
>At5g67320 unknown protein
Length = 613
Score = 28.9 bits (63), Expect = 5.6
Identities = 14/43 (32%), Positives = 27/43 (62%), Gaps = 2/43 (4%)
Query: 233 KRRSKENER--KICARKRKRKKRKRNRKRKERKFHGKKRRNKK 273
+ R+KE +R K R+R+R+K +R ++R+ K +K R ++
Sbjct: 130 RNRAKEKDRHEKQKEREREREKLEREKEREREKIEREKERERE 172
>At5g64200 unknown protein
Length = 303
Score = 28.9 bits (63), Expect = 5.6
Identities = 14/40 (35%), Positives = 23/40 (57%)
Query: 233 KRRSKENERKICARKRKRKKRKRNRKRKERKFHGKKRRNK 272
+RRS+++ R+ RKR R + R+R E K +RR +
Sbjct: 146 RRRSRDDYREKDYRKRSRSRSYDRRERHEEKDRDHRRRTR 185
>At3g05670 unknown protein
Length = 883
Score = 28.9 bits (63), Expect = 5.6
Identities = 16/38 (42%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Query: 237 KENERKICARKRKRKKRKRNRKRKERKFHGKKRRNKKC 274
+E E +I A + K + KRK R G+KRR KKC
Sbjct: 243 EEEEEEIIACNKNALKVCKGNKRKRRSGEGRKRR-KKC 279
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.385 0.173 0.749
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,800,018
Number of Sequences: 26719
Number of extensions: 233531
Number of successful extensions: 4657
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 4110
Number of HSP's gapped (non-prelim): 333
length of query: 421
length of database: 11,318,596
effective HSP length: 102
effective length of query: 319
effective length of database: 8,593,258
effective search space: 2741249302
effective search space used: 2741249302
T: 11
A: 40
X1: 13 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 35 (22.0 bits)
S2: 61 (28.1 bits)
Medicago: description of AC131249.9