BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC130803.17 + phase: 0
(532 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g53020 putative protein 33 0.39
At5g03060 putative protein 33 0.39
At3g51730 unknown protein 33 0.51
At1g79830 unknown protein (At1g79830) 32 0.88
At1g08730 myosin MYA1, class V (Z28389) like protein 32 0.88
At3g26130 cellulase, putative 32 1.1
At4g36990 heat shock transcription factor HSF4 31 2.0
At5g52280 hyaluronan mediated motility receptor-like protein 30 2.6
At4g23780 putative protein 30 3.3
At5g49030 isoleucyl-tRNA synthetase 30 4.4
At5g61490 putative protein 29 5.7
At5g49680 putative protein 29 5.7
At1g63100 transcription factor SCARECROW, putative 29 5.7
At1g47560 hypothetical protein 29 5.7
At1g16690 Unknown protein 29 5.7
At5g55520 putative protein 29 7.4
At5g13340 putative protein 29 7.4
At2g25010 unknown protein 29 7.4
At1g13130 unknown protein 29 7.4
At1g73860 kinesin-related protein 28 9.7
>At5g53020 putative protein
Length = 720
Score = 33.1 bits (74), Expect = 0.39
Identities = 20/72 (27%), Positives = 35/72 (47%), Gaps = 9/72 (12%)
Query: 445 MESHEKQVWKAR---------YNQAENLIMTLDGRDEQKTHENLMLKKELAKARKELEEK 495
++ H ++W+ + Q E + + + E K HE L E+ K RK+LE+K
Sbjct: 154 VQEHSSELWRQKKTFLELASSQRQLEAELSRANKQIEAKGHELEDLSLEINKMRKDLEQK 213
Query: 496 DELLLRDSKRAK 507
D +L K++K
Sbjct: 214 DRILAVMMKKSK 225
Score = 28.5 bits (62), Expect = 9.7
Identities = 23/85 (27%), Positives = 41/85 (48%), Gaps = 7/85 (8%)
Query: 449 EKQVW-KARYN---QAENLIMTLDGRDEQKTHENLMLKKELAKARKELEEKDELLLRDSK 504
+KQ W + RY Q E L+ L +++K H L L++ L + LE KD+ + + +
Sbjct: 68 QKQNWLRERYGLRLQIEALMKELRNIEKRKRHSLLELQERLKEKEGLLESKDKAIEEEKR 127
Query: 505 RAKGRRDFFARYCGSDSESDDLPTT 529
+ + + R ++ E DL T
Sbjct: 128 KCELLEE---RLVKAEKEVQDLRET 149
>At5g03060 putative protein
Length = 292
Score = 33.1 bits (74), Expect = 0.39
Identities = 20/76 (26%), Positives = 37/76 (48%), Gaps = 2/76 (2%)
Query: 440 QEHLAMESHEKQVWKARYNQAENLIMTLDGRDEQKTHENLMLKKELAKARKELEEKDELL 499
QE + HE + K Y E +++ +Q N ++K + K KELEEK L
Sbjct: 31 QEEILATKHE--ILKENYENLEKDYKSIEESFKQMNEMNEIMKFQYQKQIKELEEKILSL 88
Query: 500 LRDSKRAKGRRDFFAR 515
L+D ++ + ++ + +
Sbjct: 89 LKDLEKERSEKEEYMK 104
>At3g51730 unknown protein
Length = 213
Score = 32.7 bits (73), Expect = 0.51
Identities = 25/76 (32%), Positives = 33/76 (42%), Gaps = 17/76 (22%)
Query: 209 LLIYGLILFPNLDNFVDMNAIEVFHSKNPVPTLL-----------------ADTYHAIHD 251
LL+ GLIL + +FVD E +K V TL A+ +HD
Sbjct: 10 LLLLGLILVSDARSFVDSTISEKVSNKEDVCTLCEEYVTDALSYLEKNVTQAEIIEDLHD 69
Query: 252 RTLKGRGYILCCISLL 267
R + RGY CISL+
Sbjct: 70 RCSQLRGYSQQCISLV 85
>At1g79830 unknown protein (At1g79830)
Length = 918
Score = 32.0 bits (71), Expect = 0.88
Identities = 31/109 (28%), Positives = 48/109 (43%), Gaps = 4/109 (3%)
Query: 423 STPSIPLPL--LPATQDMYQEHLAMESHEKQVWKARYNQAENLIMTLDGRDEQKTHENLM 480
S PS P+ L L A Q + L E + Y A+ TL+GR Q E
Sbjct: 670 SLPSSPIQLSCLRAEQGQLSKSLEKERQRAAENRQEYLAAKEEADTLEGRANQLEVEIRE 729
Query: 481 LKKELAKARKELEEKDELLLRDSKRAKGRRDFFARYC--GSDSESDDLP 527
L+++ + +E+ +EL+ +D +R K R R S + S+ LP
Sbjct: 730 LRRKHKQELQEVLLHNELIQKDLEREKASRLDLERTARINSSAVSEQLP 778
>At1g08730 myosin MYA1, class V (Z28389) like protein
Length = 1572
Score = 32.0 bits (71), Expect = 0.88
Identities = 15/37 (40%), Positives = 24/37 (64%)
Query: 471 DEQKTHENLMLKKELAKARKELEEKDELLLRDSKRAK 507
+E KT E L LK + RK+++E + LLL++ + AK
Sbjct: 958 EEAKTQEILKLKSSFEEMRKKVDETNALLLKEREAAK 994
>At3g26130 cellulase, putative
Length = 551
Score = 31.6 bits (70), Expect = 1.1
Identities = 12/25 (48%), Positives = 18/25 (72%)
Query: 2 VAINYYVREDKLQLDESFGVVDATW 26
+A +YY+RE + DES+GV+D W
Sbjct: 343 LAGSYYIREKSIGSDESYGVLDFNW 367
>At4g36990 heat shock transcription factor HSF4
Length = 284
Score = 30.8 bits (68), Expect = 2.0
Identities = 14/34 (41%), Positives = 21/34 (61%)
Query: 461 ENLIMTLDGRDEQKTHENLMLKKELAKARKELEE 494
EN++ L G +E+ EN L ELA A+K+ +E
Sbjct: 150 ENMVADLSGENEKLKRENNNLSSELAAAKKQRDE 183
>At5g52280 hyaluronan mediated motility receptor-like protein
Length = 853
Score = 30.4 bits (67), Expect = 2.6
Identities = 30/153 (19%), Positives = 62/153 (39%), Gaps = 7/153 (4%)
Query: 363 TNAPTGQRKAFMDAWSKVRRKSVKHLGVRSGVAHEAYTQWVIDRAEEIGMPYPAMRYVSS 422
++A + + + W K R + + L + VA A + + ++ +R + +
Sbjct: 671 SSAAATETEKIIQEWRKERDEFERKLSLAKEVAKTAQKELTLTKSSNDDKE-TRLRNLKT 729
Query: 423 STPSIPLPLLPATQDMYQEHLAMESHEKQVWKARYN---QAENLIMTLDGRDEQKTHENL 479
+ L QE + + KQV + + + E + LD R E ++ EN
Sbjct: 730 EVEGLSLQYSELQNSFVQEKMENDELRKQVSNLKVDIRRKEEEMTKILDARMEARSQENG 789
Query: 480 MLKKELAKARKEL---EEKDELLLRDSKRAKGR 509
++ L+K EL + K+ + R+ K + R
Sbjct: 790 HKEENLSKLSDELAYCKNKNSSMERELKEMEER 822
>At4g23780 putative protein
Length = 148
Score = 30.0 bits (66), Expect = 3.3
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 14/75 (18%)
Query: 435 TQDMYQEHLAMESHEKQVWKARYNQAENLIMTLDGRDEQKTHENLMLKKELAKARKELEE 494
+ + + +++ + HE +WK +Y E+L+ HENL+ + E EL E
Sbjct: 12 SNNKFSDNVPLAQHE--IWKEKY---ESLVTE---------HENLVKEHETLIKTLELIE 57
Query: 495 KDELLLRDSKRAKGR 509
K E+ L D + K R
Sbjct: 58 KHEITLEDLVKKKQR 72
>At5g49030 isoleucyl-tRNA synthetase
Length = 1093
Score = 29.6 bits (65), Expect = 4.4
Identities = 21/59 (35%), Positives = 27/59 (45%), Gaps = 3/59 (5%)
Query: 368 GQRKAFMDAWSKVRRKSVKHLGV-RSGVAHEAYTQWVIDRAEEIGMPYPAMRYVSSSTP 425
G R A MDA + V K V H V R + + W I R G+P PA +V + P
Sbjct: 548 GFRTATMDAINNV--KWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHVKTKEP 604
>At5g61490 putative protein
Length = 260
Score = 29.3 bits (64), Expect = 5.7
Identities = 14/42 (33%), Positives = 23/42 (54%)
Query: 261 LCCISLLYRWFISHLPSSFHDNSENWSYSQRIMALTPNEVVW 302
LC SLL + + L +FH + S + +M+LT ++ VW
Sbjct: 118 LCNTSLLQPFMLDRLYDAFHVFQTDPSVQRMVMSLTSDKAVW 159
>At5g49680 putative protein
Length = 1378
Score = 29.3 bits (64), Expect = 5.7
Identities = 20/77 (25%), Positives = 36/77 (45%), Gaps = 7/77 (9%)
Query: 455 ARYNQAENLIMTLDGRDEQKTHENL-------MLKKELAKARKELEEKDELLLRDSKRAK 507
AR +A N + L G ++ + E + + + ELAK E +E+D ++L D R
Sbjct: 765 ARLPKAHNDSLKLSGEEDDEVEEEIDEVVPDGIEEVELAKIELEAKERDRMMLLDDIRKL 824
Query: 508 GRRDFFARYCGSDSESD 524
+ + + + ESD
Sbjct: 825 TQNESNSGNINLEKESD 841
>At1g63100 transcription factor SCARECROW, putative
Length = 658
Score = 29.3 bits (64), Expect = 5.7
Identities = 17/44 (38%), Positives = 23/44 (51%), Gaps = 3/44 (6%)
Query: 487 KARKELEEKDELLLRDSKRAKGRRD---FFARYCGSDSESDDLP 527
K+ K L E+DE L +KR K + +F + G DS S LP
Sbjct: 117 KSLKRLAEEDESCLSRAKRTKCENEGGFWFEHFTGQDSSSPALP 160
>At1g47560 hypothetical protein
Length = 1564
Score = 29.3 bits (64), Expect = 5.7
Identities = 21/72 (29%), Positives = 30/72 (41%), Gaps = 3/72 (4%)
Query: 454 KARYNQAENLIMTLDGRDEQKTHENLMLKKELAKARKELEEKDELLLRDSKRAKG---RR 510
+ Y E + L + E + L +RKE EEK L+ +KR+ G +
Sbjct: 1431 QVNYFDREEFVKKLQAELDISVREEINQSWYLRISRKEAEEKCYTYLQWAKRSSGTKRAK 1490
Query: 511 DFFARYCGSDSE 522
DF R G D E
Sbjct: 1491 DFHGRANGDDEE 1502
>At1g16690 Unknown protein
Length = 439
Score = 29.3 bits (64), Expect = 5.7
Identities = 42/164 (25%), Positives = 72/164 (43%), Gaps = 23/164 (14%)
Query: 383 KSVKHLGVRSGVAHEAYTQWVIDRAEEIGMPYPAMRYVSSSTP-------SIPLP----- 430
+++K+L ++ GV H Y+ W R EI P +R + P ++ P
Sbjct: 173 EALKYLSIKYGVFHAIYSYWKNKR--EIWQK-PILRRLQPPPPVNDTNPYNVFRPREKAH 229
Query: 431 LLPATQDMYQEHLAMESHEKQVWKARYNQAENLIMTLDGRDEQKTHENLMLKKELAKARK 490
L A + +E+ A + + + +QA+ ++ L R+E+K + E++ R
Sbjct: 230 RLHARRMQQRENNAQSFEKLRQVRRNLDQAKTILEALIKREEKKRD---FMASEVSLQRI 286
Query: 491 ELEEKDEL-LLRDSKRAKGRRDFFARYCGSDS----ESDDLPTT 529
+L+ K+E LL DS G A GS +SDD TT
Sbjct: 287 QLKYKNETELLEDSLALAGFPLSTAYRFGSSEDEFMDSDDPTTT 330
>At5g55520 putative protein
Length = 802
Score = 28.9 bits (63), Expect = 7.4
Identities = 16/59 (27%), Positives = 30/59 (50%), Gaps = 4/59 (6%)
Query: 445 MESHEKQVWKARYNQAENLIMTLDGRDE----QKTHENLMLKKELAKARKELEEKDELL 499
++ ++K + + L+ + DG DE ++T+E +E K R +L EK+ LL
Sbjct: 431 LDEYKKNALQESSKVTQQLMKSDDGEDETEVVKETYETNQRSEEFGKVRIDLSEKEALL 489
>At5g13340 putative protein
Length = 242
Score = 28.9 bits (63), Expect = 7.4
Identities = 21/68 (30%), Positives = 40/68 (57%), Gaps = 7/68 (10%)
Query: 445 MESHEKQVWKARYNQAENLIMTLDGRDEQKTHENLMLK--KELAKARKELEEKDELLLRD 502
+E + ++V +++ +A M L ++E++ E +L+ KE A RK+LEE++E +
Sbjct: 169 LEENSRRVEESQRREA----MELQRKEEERYRELELLQRQKEEAARRKKLEEEEE-IRNS 223
Query: 503 SKRAKGRR 510
SK + G R
Sbjct: 224 SKLSNGNR 231
>At2g25010 unknown protein
Length = 509
Score = 28.9 bits (63), Expect = 7.4
Identities = 35/140 (25%), Positives = 57/140 (40%), Gaps = 35/140 (25%)
Query: 242 LADTYHAIHDRTLKGRGYILCCISLLYRWFISHLPSSFHDNSEN-------W-------- 286
LA Y A+ + +LK + I C++LL W HL + SE W
Sbjct: 221 LACLYRALGNASLKSQSNICGCLTLLQCWSYFHLDIGRPEKSEACFPLALLWKGKGSRSK 280
Query: 287 ----SYSQRIMALTPNEVVW---------ITPAAQAKEIIMGCGDFLNVPLLGTRGGINY 333
Y + + L P+++ W I P +AK I+ G + + +++
Sbjct: 281 TDLSEYRRELDDLDPSKITWCPYERFENLIPPHIKAKLIL---GRSKTTLVCFEKIELHF 337
Query: 334 NPELAMRQFGFPMKSKPINL 353
P+ +RQFG K +PI L
Sbjct: 338 -PDRCLRQFG---KRQPIPL 353
>At1g13130 unknown protein
Length = 552
Score = 28.9 bits (63), Expect = 7.4
Identities = 9/22 (40%), Positives = 18/22 (80%)
Query: 5 NYYVREDKLQLDESFGVVDATW 26
+YY+R+ K+ ++E +GV+D+ W
Sbjct: 357 SYYLRQGKVGMNEYYGVLDSDW 378
>At1g73860 kinesin-related protein
Length = 1050
Score = 28.5 bits (62), Expect = 9.7
Identities = 14/59 (23%), Positives = 32/59 (53%)
Query: 449 EKQVWKARYNQAENLIMTLDGRDEQKTHENLMLKKELAKARKELEEKDELLLRDSKRAK 507
EK +W+ + E ++ L +++Q E LK+EL +++ E++ + +K++K
Sbjct: 238 EKSMWEEKKKHEEEDMVKLMKQNDQHNLEISALKQELETTKRKYEQQYSQIESQTKKSK 296
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.320 0.136 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,575,821
Number of Sequences: 26719
Number of extensions: 503043
Number of successful extensions: 1477
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 1462
Number of HSP's gapped (non-prelim): 24
length of query: 532
length of database: 11,318,596
effective HSP length: 104
effective length of query: 428
effective length of database: 8,539,820
effective search space: 3655042960
effective search space used: 3655042960
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 62 (28.5 bits)
Medicago: description of AC130803.17