BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126784.4 - phase: 0 /pseudo
(525 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g30780 unknown protein 394 e-110
At2g24100 Unknown protein 374 e-104
At3g05770 unknown protein 133 3e-31
At1g54300 hypothetical protein 115 8e-26
At1g07910 translation elongation factor EF-1 alpha 32 0.66
At5g60930 microtubule-associated motor - like 31 1.5
At3g49000 RNA polymerase III subunit - like protein 31 1.5
At3g20830 unknown protein 30 2.5
At1g03080 unknown protein 30 3.3
At2g32820 hypothetical protein 30 4.3
At5g46510 disease resistance protein-like 29 5.6
At2g37380 unknown protein 29 5.6
At4g24265 unknown protein 29 7.3
At4g02730 putative WD-repeat protein 29 7.3
At4g02560 LUMINIDEPENDENS protein 29 7.3
At5g02760 protein phosphatase - like protein 28 9.5
At3g10040 unknown protein 28 9.5
At2g24820 Rieske iron-sulfur like protein 28 9.5
>At4g30780 unknown protein
Length = 589
Score = 394 bits (1011), Expect = e-110
Identities = 263/595 (44%), Positives = 334/595 (55%), Gaps = 88/595 (14%)
Query: 2 VRFMGSKNPRSQWESSSATSSISPKFEI-EDSIQDQHAPLNKRHK-------------AT 47
+R G P S+ ++ + K EI ED ++++H PLNKR + A
Sbjct: 4 MRKSGGLRPESE-SAARCRDELPVKLEIAEDDLEEEHGPLNKRSRLWSPGTSSSTMAPAK 62
Query: 48 NDILNEPSPLGLSLRKSPSLLDLIQM--TLCQE----NSVNANTANDNLNSKANKNGRA- 100
+ L+EPSPLGLSL+KSPSLL+LIQM T C + ++ A L ++ A
Sbjct: 63 YNPLDEPSPLGLSLKKSPSLLELIQMKITHCGDPKAAETLKAGALGSGLKRESKTIAAAA 122
Query: 101 ---------SVEKLKASNFPATHLKIGSWEYKSKYEGDLVAKCYFAKQKLVWEVLEGELK 151
S+EKLKASNFPA+ LKIG WEYKS+YEGDLVAKCYFAK KLVWEVLE LK
Sbjct: 123 SVGPTLAPGSIEKLKASNFPASLLKIGQWEYKSRYEGDLVAKCYFAKHKLVWEVLEQGLK 182
Query: 152 SKIEIQWSDISQLKANCPDDGPSTLTLMVARQPLFFRETNPQPRKHTLWQSTTDFTGGQA 211
SKIEIQWSDI LKANCP+DGP TLTL++ARQPLFFRETNPQPRKHTLWQ+T+DFT GQA
Sbjct: 183 SKIEIQWSDIMALKANCPEDGPGTLTLVLARQPLFFRETNPQPRKHTLWQATSDFTDGQA 242
Query: 212 SIHRRHVLQCEQGLLIKHYEKLVQCNDRLKFLSQQPEIMVDSPHFDPRSAAIENPHNLKD 271
S++R+H LQC QG++ KH+EKLVQC+ RL LS+QPEI +DSP+FD R + E+P K
Sbjct: 243 SMNRQHFLQCAQGIMNKHFEKLVQCDHRLFHLSRQPEIAIDSPYFDARQSIFEDPSESKG 302
Query: 272 ---CDLHQGNGSAVSCFQNMGSPHSSLSPS----FTTEHSDPSAI--------------- 309
+L+ G ++S QN+ SP + S S + E PS++
Sbjct: 303 HPFGNLNLSTGPSISGTQNLASPVGAQSSSEHMYLSHEAPSPSSVIDARANEGIGGSEAV 362
Query: 310 ----TLDSVPCEAPS-SSSA*IMKFSSKIC------QMSII*NL**LCKSVIVVLAC*FH 358
D EAP S + F + +C +++ ++ L +S+ V +
Sbjct: 363 NSRNRTDCGQIEAPGIHQSMSLSDFLAVLCDTKNTTDLNLADDVDGLHQSMSVSDFVAY- 421
Query: 359 Q*FNMLGSRN---WDQIKLPGLRPSMSMSDFLGHIEHHISKEMASGDPSFSAERLEYQQM 415
+ SRN DQIK+PGL SMS+SDF+G + + A G E+ E +
Sbjct: 422 ----LSDSRNITDSDQIKVPGLHQSMSVSDFVG-----LLSDSAGGSHPEHMEKFEIMK- 471
Query: 416 MDGITQHLLNDNQVTTDSDEKSLMSRVNSLRCLLQMDPPAVPNSHDNTGFIEGPNDAKVN 475
Q LL+DN DEKSLM RVNSL LL DP NS NT G
Sbjct: 472 -----QQLLSDNIQFEAPDEKSLMPRVNSLFNLLYKDPNVAANSQLNTEMSVGLKSEPKG 526
Query: 476 IDIKATEENSR-----DVYGGNPAPGMSRKDSFGDLLLSLPRIASLPKFLFDISE 525
I N+ D + GM RKDSF DLLL LPRI SLPKFL +ISE
Sbjct: 527 IVSDNNNNNNNNNRVLDTASSSKPQGMLRKDSFSDLLLHLPRITSLPKFLSNISE 581
>At2g24100 Unknown protein
Length = 463
Score = 374 bits (961), Expect = e-104
Identities = 231/508 (45%), Positives = 292/508 (57%), Gaps = 83/508 (16%)
Query: 29 IEDSIQDQHAPLNKRHKATND--------ILNEPSPLGLSLRKSPSLLDLIQMTLCQENS 80
IEDS++++HAPLNKR K ++ +L EPSPLGLSL+KSPS +LI+M L Q
Sbjct: 26 IEDSLEEEHAPLNKRSKLWSNGTSVSKFSLLEEPSPLGLSLKKSPSFQELIEMKLSQ--- 82
Query: 81 VNANTANDNLNSKANKNGRASVEKLKASNFPATHLKIGSWEYKSKYEGDLVAKCYFAKQK 140
+ + +N G +VEKLKASNFPAT L+IG WEYKS+YEGDLVAKCYFAK K
Sbjct: 83 -SGDDSNSVKKESFGFGGVGTVEKLKASNFPATILRIGQWEYKSRYEGDLVAKCYFAKHK 141
Query: 141 LVWEVLEGELKSKIEIQWSDISQLKANCPDDGPSTLTLMVARQPLFFRETNPQPRKHTLW 200
LVWEVLE LKSKIEIQWSDI LKAN P+D P TLT+++AR+PLFFRETNPQPRKHTLW
Sbjct: 142 LVWEVLEQGLKSKIEIQWSDIMALKANLPEDEPGTLTIVLARRPLFFRETNPQPRKHTLW 201
Query: 201 QSTTDFTGGQASIHRRHVLQCEQGLLIKHYEKLVQCNDRLKFLSQQPEIMVDSPHFDPRS 260
Q+T+DFT GQAS++R+H LQC G++ KH+EKLVQC+ RL LS+QPEI + +P FD R
Sbjct: 202 QATSDFTDGQASMNRQHFLQCPPGIMNKHFEKLVQCDHRLFCLSRQPEINLAAPFFDSRL 261
Query: 261 AAIENPHNLKDCDLHQGNGSAVSCFQNMGSPHSSLSPSFTTEHSDPSAITLDSVPCEAPS 320
+ E+P ++ G A S +++ H +LSPS +D+ E
Sbjct: 262 SIFEDPSVSGSHNIASPVG-AQSSSEHVSLSHDALSPS----------SVMDARAIEGVG 310
Query: 321 SSSA*IMKFSSKICQMSII*NL**LCKSVIVVLAC*FHQ*FNMLGSRNWDQIKLPGLRPS 380
S + + W QIK+PGL S
Sbjct: 311 GS--------------------------------------IDSRNTNGWSQIKMPGLHQS 332
Query: 381 MSMSDFLGHIEHHISKEMASGDPSFSAERLEYQQMMDGITQHLLNDNQVTTDSDEKSLMS 440
+SM+DFL + S + E Q + + Q LL+DN T SDEKS+MS
Sbjct: 333 ISMNDFLTFL---------------SDQACENNQEFEEMKQLLLSDNTQTDPSDEKSVMS 377
Query: 441 RVNSLRCLLQMDPPAVPNSHDNTGFIEGPNDAKVNIDIKATEENSRDV---YGGNPAPGM 497
+VNS LLQ + NS N + V+ + E R V P GM
Sbjct: 378 KVNSFCNLLQ----SAANSQLNIETADTERVVGVDNNRHMPEGGKRVVDPASSSKPLQGM 433
Query: 498 SRKDSFGDLLLSLPRIASLPKFLFDISE 525
SRKDSF DLL+ LPRI SLPKFLF+ISE
Sbjct: 434 SRKDSFSDLLVHLPRITSLPKFLFNISE 461
>At3g05770 unknown protein
Length = 410
Score = 133 bits (334), Expect = 3e-31
Identities = 85/197 (43%), Positives = 112/197 (56%), Gaps = 14/197 (7%)
Query: 48 NDILNEPSPLGLSLRKSPSLLDLIQMTL-CQENSVNANTANDNLNSKANKNGRASVEKLK 106
N ++E L L L K+P L++ I+ L + T N + S K S EKLK
Sbjct: 17 NRFVDEGPRLNLPLTKTPELINKIESYLKVHYTCPHQQTENSSKTSTLPK----SPEKLK 72
Query: 107 ASNFPATHLKIGSWEYKSKYEGDLVAKCYFAKQKLVWEVLEGE-------LKSKIEIQWS 159
A NFP + +KIG + +K D+VAK YFAK+KL+WE L GE LKSKIEIQW+
Sbjct: 73 AMNFPISTIKIGDCVFVAKNPDDIVAKFYFAKKKLLWEFLFGEPVANMPRLKSKIEIQWN 132
Query: 160 DISQLKANCPD-DGPSTLTLMVARQPLFFRETNPQPRKHTLW-QSTTDFTGGQASIHRRH 217
D+S + + D L + + ++P FF ETNPQ KHT W Q DFTG QAS +RRH
Sbjct: 133 DVSSFEESINSRDETGILKIELKKRPTFFTETNPQAGKHTQWKQLDYDFTGDQASYYRRH 192
Query: 218 VLQCEQGLLIKHYEKLV 234
L G+L K+ EKL+
Sbjct: 193 TLHFPPGVLQKNLEKLL 209
>At1g54300 hypothetical protein
Length = 314
Score = 115 bits (287), Expect = 8e-26
Identities = 70/158 (44%), Positives = 93/158 (58%), Gaps = 10/158 (6%)
Query: 109 NFPATHLKIGSWEYKSKYEGDLVAKCYFAKQKLVWEVLEGE-------LKSKIEIQWSDI 161
NFP + ++IG W +K D+VAK YFAK+KL+WE L GE LK KIEIQW+D+
Sbjct: 2 NFPISTIRIGGWVVVAKNPDDIVAKFYFAKKKLIWEFLFGEPETNTLRLKRKIEIQWNDV 61
Query: 162 SQLKANCPD-DGPSTLTLMVARQPLFFRETNPQPRKHTLW-QSTTDFTGGQASIHRRHVL 219
S + + D L + + ++P FF ETNPQ KHT W Q DFTG AS +RRH L
Sbjct: 62 SSFEESISSRDETGILKIELKKRPTFFIETNPQAGKHTQWKQLDHDFTGDHASNYRRHTL 121
Query: 220 QCEQGLLIKHYEKLVQCNDRLKFLSQQPEIMVDSPHFD 257
G+L K+ EKLV + K L + P + +S +FD
Sbjct: 122 HFPPGVLQKNLEKLVTDSFWSK-LYEVPFPVHESRYFD 158
>At1g07910 translation elongation factor EF-1 alpha
Length = 1081
Score = 32.3 bits (72), Expect = 0.66
Identities = 30/156 (19%), Positives = 61/156 (38%), Gaps = 18/156 (11%)
Query: 61 LRKSPSLLDLIQMTLCQENSVNANTAND-----NLNSKANKNGRASVEKLKASNFPATHL 115
+R PSL L + N +N D ++++ + +GR + L A + +
Sbjct: 459 MRHMPSLWPLYRGFFVDINLFKSNKGRDLMALKSIDNASENDGRGEKDGL-ADDDANLMI 517
Query: 116 KIGSWEYKSKY------------EGDLVAKCYFAKQKLVWEVLEGELKSKIEIQWSDISQ 163
K+ YK + +G K Y+ +Q +W +G+ K ++ +
Sbjct: 518 KMKFLTYKLRTFLIRNGLSILFKDGAAAYKTYYLRQMKIWGTSDGKQKELCKMLDEWAAY 577
Query: 164 LKANCPDDGPSTLTLMVARQPLFFRETNPQPRKHTL 199
++ C +D S+ T + +P + P+ H L
Sbjct: 578 IRRKCGNDQLSSSTYLSEAEPFLEQYAKRSPKNHIL 613
>At5g60930 microtubule-associated motor - like
Length = 647
Score = 31.2 bits (69), Expect = 1.5
Identities = 15/38 (39%), Positives = 20/38 (52%)
Query: 448 LLQMDPPAVPNSHDNTGFIEGPNDAKVNIDIKATEENS 485
L+ + PPA+P +H NT I N V+ D ENS
Sbjct: 541 LVPVGPPALPPTHTNTHLIPEANSVTVDSDTARMPENS 578
>At3g49000 RNA polymerase III subunit - like protein
Length = 523
Score = 31.2 bits (69), Expect = 1.5
Identities = 19/71 (26%), Positives = 33/71 (45%)
Query: 39 PLNKRHKATNDILNEPSPLGLSLRKSPSLLDLIQMTLCQENSVNANTANDNLNSKANKNG 98
P +R IL EP L + ++ + +D I+ L E N++ A+DN N K
Sbjct: 178 PAKRRGAKAAKILKEPETLEEQVVEAATPVDAIRFPLIFEEDSNSSLADDNSNITEGKRK 237
Query: 99 RASVEKLKASN 109
+ V+ +S+
Sbjct: 238 QRDVDTSDSSS 248
>At3g20830 unknown protein
Length = 408
Score = 30.4 bits (67), Expect = 2.5
Identities = 13/36 (36%), Positives = 21/36 (58%)
Query: 488 VYGGNPAPGMSRKDSFGDLLLSLPRIASLPKFLFDI 523
+YG P G S+K++F ++L+ P A P L D+
Sbjct: 278 MYGETPFKGKSKKETFRNVLMKEPEFAGKPNDLTDL 313
>At1g03080 unknown protein
Length = 1744
Score = 30.0 bits (66), Expect = 3.3
Identities = 11/25 (44%), Positives = 20/25 (80%)
Query: 422 HLLNDNQVTTDSDEKSLMSRVNSLR 446
HLLND++ T S+ +SL+S ++++R
Sbjct: 765 HLLNDDKTTLTSERESLLSHIDTMR 789
>At2g32820 hypothetical protein
Length = 333
Score = 29.6 bits (65), Expect = 4.3
Identities = 24/79 (30%), Positives = 34/79 (42%), Gaps = 2/79 (2%)
Query: 6 GSKNPRSQWESSSATSSISPKFEIEDSIQDQHAPLN-KRHKATNDILNEPSPLGLSLRKS 64
GSKNPRS + I K + PLN K+H+ N + L L + S
Sbjct: 186 GSKNPRSGAGETGVIKKILRKPDFCCKSVPSRPPLNMKKHQPVKAFENPKTCLVLK-KNS 244
Query: 65 PSLLDLIQMTLCQENSVNA 83
P +L+L +M + NA
Sbjct: 245 PEMLELFEMAKKSADVANA 263
>At5g46510 disease resistance protein-like
Length = 1353
Score = 29.3 bits (64), Expect = 5.6
Identities = 22/89 (24%), Positives = 39/89 (43%), Gaps = 4/89 (4%)
Query: 363 MLGSRNWDQIKLPGLRPSMSMSDFLGHIEHHISKEMASGDPSFSAERLEYQQMMDGITQH 422
M SRNW + PS S +D + H+ K + SF + +E Q +D + +
Sbjct: 1 MASSRNWVY----DVFPSFSGTDVRRNFLSHLLKGLHKSVNSFRDQNMERSQSLDPMLKQ 56
Query: 423 LLNDNQVTTDSDEKSLMSRVNSLRCLLQM 451
+ D+++ K+ S L LL++
Sbjct: 57 AIRDSRIALVVFSKNYASSSWCLNELLEI 85
>At2g37380 unknown protein
Length = 321
Score = 29.3 bits (64), Expect = 5.6
Identities = 22/96 (22%), Positives = 44/96 (44%), Gaps = 10/96 (10%)
Query: 15 ESSSATSSISPKFEIEDSIQDQHAPLNKRHKATNDILNEPSPLGLSLRKSPSLLDLIQMT 74
++ S +S I+P+F+IE S K + N +N +PL + R ++
Sbjct: 189 QACSDSSEINPRFKIEPS---------KVSRKKNPFVNSENPLLIHRRSFSGVIQRHSQA 239
Query: 75 LCQENSVNANTANDNLNS-KANKNGRASVEKLKASN 109
C +S ++++A+ +S NG ++ L S+
Sbjct: 240 KCSTSSSSSSSASSLSSSFSFGSNGSLDLQTLMRSS 275
>At4g24265 unknown protein
Length = 140
Score = 28.9 bits (63), Expect = 7.3
Identities = 20/59 (33%), Positives = 27/59 (44%), Gaps = 1/59 (1%)
Query: 420 TQHLLNDNQVTTDSDEKSLMSRVNSLRCLLQMDPPAVPNSHDNTGFIEGPNDAKVNIDI 478
T H L Q T + L+S+V+ LR VPN N F+E P AK ++ I
Sbjct: 66 TTHHLPHQQEETIVEATPLLSQVSDLRIYQSYQQYMVPNPTSNVHFVE-PATAKRSVGI 123
>At4g02730 putative WD-repeat protein
Length = 333
Score = 28.9 bits (63), Expect = 7.3
Identities = 29/120 (24%), Positives = 41/120 (34%), Gaps = 22/120 (18%)
Query: 79 NSVNANTANDNLNSKANKNGRASVEKLKASNFPATHLKIGSWE------YKSKYEGDLVA 132
N + AN N A +G + K P HLK K +G+L+A
Sbjct: 6 NGTSNGVANANSTGNAGTSGNVPIYK------PYRHLKTLEGHTAAISCVKFSNDGNLLA 59
Query: 133 KCYFAKQKLVWEV--------LEGELKSKIEIQWSDISQLKANCPDDGPSTLTLMVARQP 184
K ++W EG ++ WS S + DD TL + AR P
Sbjct: 60 SASVDKTMILWSATNYSLIHRYEGHSSGISDLAWSSDSHYTCSASDD--CTLRIWDARSP 117
>At4g02560 LUMINIDEPENDENS protein
Length = 953
Score = 28.9 bits (63), Expect = 7.3
Identities = 33/133 (24%), Positives = 54/133 (39%), Gaps = 21/133 (15%)
Query: 9 NPRSQWE-----SSSATSSISPKFEIEDSIQDQHAPLNKRHKA-----TNDILNEPSP-- 56
NP+ W+ S T I + E+S+ + L++R A T+ L+ P P
Sbjct: 634 NPKEPWDREMDYDDSLTPEIPSQQPPEESLTEPQDSLDERRIAAGAATTSSSLSSPEPDL 693
Query: 57 -LGLSLRKSPSLLDLIQM----TLCQENSVNA----NTANDNLNSKANKNGRASVEKLKA 107
L +L K+P L+ + L ++ V T N +S +NK VE
Sbjct: 694 ELLAALLKNPDLVYALTSGKPSNLAGQDMVKLLDVIKTGAPNSSSSSNKQVEERVEVSLP 753
Query: 108 SNFPATHLKIGSW 120
S P+T+ + W
Sbjct: 754 SPTPSTNPGMSGW 766
>At5g02760 protein phosphatase - like protein
Length = 361
Score = 28.5 bits (62), Expect = 9.5
Identities = 25/96 (26%), Positives = 41/96 (42%), Gaps = 13/96 (13%)
Query: 118 GSWEYKSKYEG-DLVAKCYFAKQKLVWEVLEGELKS---KIEIQWSDISQLKANCPDDGP 173
G WE+ S E D+V +Q + +L+ LK K E+++SD++++
Sbjct: 268 GLWEHLSNQEAVDIVHNS--PRQGIARRLLKAALKEAAKKREMRYSDLTEIHPGVRRHFH 325
Query: 174 STLTLMVARQPLFFRETNPQPRKHTLWQSTTDFTGG 209
+T++V NP P K W S GG
Sbjct: 326 DDITVIVVY-------LNPHPVKTNSWASPLSIRGG 354
>At3g10040 unknown protein
Length = 418
Score = 28.5 bits (62), Expect = 9.5
Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 10/53 (18%)
Query: 6 GSKNPRSQWESSSATS-----SISPKFEIEDSIQDQHAPLNKRHKATNDILNE 53
G + +W+S S S+SP+ + ED D LNKR+K NDIL +
Sbjct: 135 GMLQKKGKWKSVSRAMVEKGFSVSPQ-QCEDKFND----LNKRYKRVNDILGK 182
>At2g24820 Rieske iron-sulfur like protein
Length = 539
Score = 28.5 bits (62), Expect = 9.5
Identities = 16/40 (40%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Query: 451 MDPPAVPNSHDNTGFIEGPNDAKVNIDIKATEENSRDVYG 490
MDP VP SHD T F DA+ + + TE ++R G
Sbjct: 238 MDPAHVPISHDRTDFTAKREDAQPLV-FEVTERSNRGFAG 276
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.322 0.135 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,216,676
Number of Sequences: 26719
Number of extensions: 532651
Number of successful extensions: 1446
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 1419
Number of HSP's gapped (non-prelim): 21
length of query: 525
length of database: 11,318,596
effective HSP length: 104
effective length of query: 421
effective length of database: 8,539,820
effective search space: 3595264220
effective search space used: 3595264220
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)
Medicago: description of AC126784.4