BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148819.7 - phase: 0
(343 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86077 similar to GP|7208777|emb|CAB76911.1 putative PTS protei... 309 9e-85
TC84648 similar to GP|8843816|dbj|BAA97364.1 emb|CAB76911.1~gene... 142 2e-70
TC86076 similar to GP|7208777|emb|CAB76911.1 putative PTS protei... 153 8e-38
BG648645 similar to GP|18844963|db hypothetical protein {Oryza s... 49 3e-06
AJ496961 GP|7208777|emb putative PTS protein {Cicer arietinum}, ... 31 0.79
BG649088 30 1.0
TC87592 similar to GP|7331193|gb|AAF60315.1| putative purple aci... 30 1.4
TC88192 weakly similar to PIR|T05682|T05682 monooxygenase (EC 1.... 29 3.0
TC88032 similar to GP|9294381|dbj|BAB02391.1 gene_id:MIE1.9~unkn... 29 3.0
TC88804 similar to GP|7298989|gb|AAF54192.1| CG7878 gene product... 28 3.9
CA921080 28 3.9
TC78476 homologue to SP|O22437|CHLD_PEA Magnesium-chelatase subu... 28 6.7
TC88244 homologue to GP|7385201|gb|AAF61730.1| beta-ketoacyl-ACP... 27 8.8
TC78600 similar to GP|10334495|emb|CAC10209. putative extracellu... 27 8.8
TC89825 weakly similar to GP|17473561|gb|AAL38256.1 putative pro... 27 8.8
TC91222 weakly similar to GP|20977602|gb|AAM28228.1 histone acet... 27 8.8
>TC86077 similar to GP|7208777|emb|CAB76911.1 putative PTS protein {Cicer
arietinum}, partial (98%)
Length = 1877
Score = 309 bits (792), Expect = 9e-85
Identities = 164/345 (47%), Positives = 212/345 (60%), Gaps = 8/345 (2%)
Frame = +2
Query: 1 MHFGNG-ITKCRDVLASEFEFCSDLNTTLFLKRVIQDETPDFIAFTGDNIFGPSSHDAAE 59
MH+ NG T C DVL S+ C+DLNTT F+ R+I E P+ I FTGDNI+G S D+A+
Sbjct: 500 MHYANGKTTLCLDVLPSQKASCTDLNTTAFIHRMILAEKPNLIVFTGDNIYGYDSSDSAK 679
Query: 60 SMFKAFGPAMESGLPWAAILGNHDQESTLNREELMSLISLMDYSVSQINPSADSLTNSAK 119
SM AF PA+ES +PW A+LGNHDQE +L+RE +M I + S+S++NP
Sbjct: 680 SMNAAFAPAIESNIPWVAVLGNHDQEGSLSREGVMKYIVGLKNSLSKVNPP--------- 832
Query: 120 GHKMSKIDGFGNYNLRVYGAPGSMMANSSVLNLFFLDSGDRVVYQGIRTYDWIKDSQLHW 179
++ IDGFGN NL + G G++ N SVLNL+FLDSGD I YDWIK SQ W
Sbjct: 833 --EVHIIDGFGNNNLEIGGVQGTVFENKSVLNLYFLDSGDYSKVPTIPGYDWIKPSQQLW 1006
Query: 180 LRHVSQE-PQAQEQDPLHSTDHVTSPITPPALAFFHIPIPEVRQLFYKQIVGQFQE---- 234
S + +A + P + P LA+FHIP+PE + G E
Sbjct: 1007FERTSAKLRKAYIKGPAPQKE------AAPGLAYFHIPLPEYTSFDSSNMTGVKMETDGG 1168
Query: 235 -GVACSRVNSAVLQTFVSMGDVKAVFIGHDHTNDFCGNLDGIWFCYGGGFGYHGYGKAGW 293
G++ + VNS T V GDVKAVF+GHDH NDFCG L I CY GGFGYH YGKAGW
Sbjct: 1169DGISSASVNSGFFTTLVGAGDVKAVFVGHDHLNDFCGKLMDIQLCYAGGFGYHAYGKAGW 1348
Query: 294 PRRARIILAELQK-GKESWTSVQKIMTWKRLDDEKMSKIDEQILW 337
RRAR+++A L+K K SW +V+ I +WKRLDD+ ++ ID ++LW
Sbjct: 1349SRRARVVVASLEKTDKGSWGAVKSIKSWKRLDDQHLTGIDGEVLW 1483
>TC84648 similar to GP|8843816|dbj|BAA97364.1
emb|CAB76911.1~gene_id:MUL3.9~similar to unknown protein
{Arabidopsis thaliana}, partial (16%)
Length = 552
Score = 142 bits (357), Expect(2) = 2e-70
Identities = 66/72 (91%), Positives = 66/72 (91%), Gaps = 4/72 (5%)
Frame = +1
Query: 276 WFCYG----GGFGYHGYGKAGWPRRARIILAELQKGKESWTSVQKIMTWKRLDDEKMSKI 331
W YG GGFGYHGYGKAGWPRRARIILAELQKGKESWTSVQKIMTWKRLDDEKMSKI
Sbjct: 178 WMAYGFVMAGGFGYHGYGKAGWPRRARIILAELQKGKESWTSVQKIMTWKRLDDEKMSKI 357
Query: 332 DEQILWDHLHSR 343
DEQILWDHLHSR
Sbjct: 358 DEQILWDHLHSR 393
Score = 141 bits (356), Expect(2) = 2e-70
Identities = 65/65 (100%), Positives = 65/65 (100%)
Frame = +3
Query: 216 PIPEVRQLFYKQIVGQFQEGVACSRVNSAVLQTFVSMGDVKAVFIGHDHTNDFCGNLDGI 275
PIPEVRQLFYKQIVGQFQEGVACSRVNSAVLQTFVSMGDVKAVFIGHDHTNDFCGNLDGI
Sbjct: 9 PIPEVRQLFYKQIVGQFQEGVACSRVNSAVLQTFVSMGDVKAVFIGHDHTNDFCGNLDGI 188
Query: 276 WFCYG 280
WFCYG
Sbjct: 189 WFCYG 203
>TC86076 similar to GP|7208777|emb|CAB76911.1 putative PTS protein {Cicer
arietinum}, partial (51%)
Length = 677
Score = 153 bits (387), Expect = 8e-38
Identities = 80/160 (50%), Positives = 106/160 (66%), Gaps = 1/160 (0%)
Frame = +2
Query: 1 MHFGNGI-TKCRDVLASEFEFCSDLNTTLFLKRVIQDETPDFIAFTGDNIFGPSSHDAAE 59
MH+ NG T C +VL S+ C+DLNTT F+ R+I E P+ I FTGDNI+G S D+A+
Sbjct: 227 MHYANGKNTLCLNVLPSQNASCTDLNTTAFIHRMILAEKPNLIVFTGDNIYGHDSSDSAK 406
Query: 60 SMFKAFGPAMESGLPWAAILGNHDQESTLNREELMSLISLMDYSVSQINPSADSLTNSAK 119
SM AF PA+ES +PW A+LGNHDQE +L+RE +M I + S+S++NP
Sbjct: 407 SMDAAFAPAIESNIPWVAVLGNHDQEGSLSREGVMKYIVGLKNSLSKVNPP--------- 559
Query: 120 GHKMSKIDGFGNYNLRVYGAPGSMMANSSVLNLFFLDSGD 159
++ IDGFGN NL + G G++ N SVLNL+FLDSGD
Sbjct: 560 --EVHIIDGFGNNNLEIGGVQGTVFENKSVLNLYFLDSGD 673
>BG648645 similar to GP|18844963|db hypothetical protein {Oryza sativa
(japonica cultivar-group)}, partial (43%)
Length = 702
Score = 48.9 bits (115), Expect = 3e-06
Identities = 23/63 (36%), Positives = 36/63 (56%), Gaps = 2/63 (3%)
Frame = +2
Query: 23 DLNTTLFLKRVIQDETPDFIAFTGDNIFGPSSHDAAESMF--KAFGPAMESGLPWAAILG 80
D+N+ + V+ ETPDF+ + GD I + S++ +A PA G+PWA++ G
Sbjct: 206 DVNSINVMNTVLHHETPDFVIYLGDVITANNIMIENASLYWDQATSPARNRGIPWASVFG 385
Query: 81 NHD 83
NHD
Sbjct: 386 NHD 394
>AJ496961 GP|7208777|emb putative PTS protein {Cicer arietinum}, partial (5%)
Length = 581
Score = 30.8 bits (68), Expect = 0.79
Identities = 10/19 (52%), Positives = 16/19 (83%)
Frame = +1
Query: 319 TWKRLDDEKMSKIDEQILW 337
+WKRLDD+ ++ ID ++LW
Sbjct: 37 SWKRLDDQHLTGIDGEVLW 93
>BG649088
Length = 683
Score = 30.4 bits (67), Expect = 1.0
Identities = 12/41 (29%), Positives = 24/41 (58%)
Frame = -3
Query: 299 IILAELQKGKESWTSVQKIMTWKRLDDEKMSKIDEQILWDH 339
+I+A Q+G + + + I W+ E+++ +D +ILW H
Sbjct: 507 LIMASNQQGYDGYDNHAIIYNWRDKTVEQITSVDNEILWFH 385
>TC87592 similar to GP|7331193|gb|AAF60315.1| putative purple acid
phosphatase precursor {Ipomoea batatas}, partial (87%)
Length = 1247
Score = 30.0 bits (66), Expect = 1.4
Identities = 17/48 (35%), Positives = 21/48 (43%), Gaps = 4/48 (8%)
Frame = +3
Query: 40 DFIAFTGDNIFGPS---SHDAA-ESMFKAFGPAMESGLPWAAILGNHD 83
DF+ TGDN + HD E F A W ++LGNHD
Sbjct: 252 DFVVSTGDNFYDDGLTGEHDPNFEESFSKIYAAKSLQKQWYSVLGNHD 395
>TC88192 weakly similar to PIR|T05682|T05682 monooxygenase (EC 1.-.-.-) 2 -
Arabidopsis thaliana, partial (61%)
Length = 1557
Score = 28.9 bits (63), Expect = 3.0
Identities = 17/46 (36%), Positives = 21/46 (44%)
Frame = +3
Query: 21 CSDLNTTLFLKRVIQDETPDFIAFTGDNIFGPSSHDAAESMFKAFG 66
C DL TT ++ VIQ F+ F D I P A +FK G
Sbjct: 1155 CIDLITTAYIAGVIQQADSKFVTFFRDKILAPL---LAGQLFKKSG 1283
>TC88032 similar to GP|9294381|dbj|BAB02391.1 gene_id:MIE1.9~unknown protein
{Arabidopsis thaliana}, partial (14%)
Length = 1415
Score = 28.9 bits (63), Expect = 3.0
Identities = 20/65 (30%), Positives = 30/65 (45%), Gaps = 5/65 (7%)
Frame = +2
Query: 226 KQIVGQFQEGVACSRVNSAVLQTFVSMGDVKAVFIGHDHTNDFCGNLD-----GIWFCYG 280
K++V E C +VNS++LQ + ++ D+K H N C NL + C
Sbjct: 971 KRVVSSIAE*RMCLQVNSSILQRYEALTDIKH---SSRHINT-CNNLRKWK*LNVATCIS 1138
Query: 281 GGFGY 285
G GY
Sbjct: 1139 VGIGY 1153
>TC88804 similar to GP|7298989|gb|AAF54192.1| CG7878 gene product
{Drosophila melanogaster}, partial (2%)
Length = 1355
Score = 28.5 bits (62), Expect = 3.9
Identities = 13/41 (31%), Positives = 17/41 (40%)
Frame = -3
Query: 176 QLHWLRHVSQEPQAQEQDPLHSTDHVTSPITPPALAFFHIP 216
Q+H +H+ QEQ H VTS P H+P
Sbjct: 759 QIHGQKHIKATDSVQEQ*SWHQKSSVTSQCFDPTSQSIHLP 637
>CA921080
Length = 709
Score = 28.5 bits (62), Expect = 3.9
Identities = 11/29 (37%), Positives = 21/29 (71%)
Frame = -1
Query: 86 STLNREELMSLISLMDYSVSQINPSADSL 114
+++NR+ +SLI + D SV +++ S DS+
Sbjct: 400 TSMNRDMYLSLIKMFDRSVKEVDKSLDSM 314
>TC78476 homologue to SP|O22437|CHLD_PEA Magnesium-chelatase subunit chlD
chloroplast precursor (Mg- protoporphyrin IX chelatase),
partial (68%)
Length = 1596
Score = 27.7 bits (60), Expect = 6.7
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = -1
Query: 265 TNDFCGNLDGIWFCYGGGFG 284
++ FC +LD WFC GGG G
Sbjct: 558 SSSFCSSLDS-WFCGGGGGG 502
>TC88244 homologue to GP|7385201|gb|AAF61730.1| beta-ketoacyl-ACP synthetase
I {Glycine max}, partial (89%)
Length = 1768
Score = 27.3 bits (59), Expect = 8.8
Identities = 12/23 (52%), Positives = 12/23 (52%)
Frame = +2
Query: 276 WFCYGGGFGYHGYGKAGWPRRAR 298
WFCYG F G GK G AR
Sbjct: 902 WFCYG*RFWSFGIGKPGTCHEAR 970
>TC78600 similar to GP|10334495|emb|CAC10209. putative extracellular dermal
glycoprotein {Cicer arietinum}, partial (20%)
Length = 1546
Score = 27.3 bits (59), Expect = 8.8
Identities = 16/52 (30%), Positives = 26/52 (49%), Gaps = 2/52 (3%)
Frame = +1
Query: 113 SLTNSAKGHKMSKIDGF--GNYNLRVYGAPGSMMANSSVLNLFFLDSGDRVV 162
+++ S G + ID G R++GA + N +VL L F+D+G V
Sbjct: 1057 TISKSVNGPNVPTIDLVLKGGVEWRIFGANSMVKVNENVLCLGFVDAGSEEV 1212
>TC89825 weakly similar to GP|17473561|gb|AAL38256.1 putative protein
{Arabidopsis thaliana}, partial (37%)
Length = 619
Score = 27.3 bits (59), Expect = 8.8
Identities = 13/28 (46%), Positives = 15/28 (53%), Gaps = 1/28 (3%)
Frame = -3
Query: 182 HVSQEPQAQEQDPLHSTDHVTSP-ITPP 208
H PQ+ PLHS H SP I+PP
Sbjct: 491 HHPNNPQSDSSSPLHSFFHYISPQISPP 408
>TC91222 weakly similar to GP|20977602|gb|AAM28228.1 histone acetyl
transferase {Zea mays}, partial (20%)
Length = 647
Score = 27.3 bits (59), Expect = 8.8
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +1
Query: 137 YGAPGSMMANSSVLNLFFLDSGDRVVYQGIRTYDWI 172
+ AP S + N LN +F D G Y+G++ W+
Sbjct: 208 FDAPESFIINPVDLNSYFDDDGKIYGYEGLKINIWV 315
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.137 0.427
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,396,487
Number of Sequences: 36976
Number of extensions: 160616
Number of successful extensions: 923
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 902
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 916
length of query: 343
length of database: 9,014,727
effective HSP length: 97
effective length of query: 246
effective length of database: 5,428,055
effective search space: 1335301530
effective search space used: 1335301530
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC148819.7