
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148718.5 + phase: 0
(344 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW683633 PIR|T47870|T47 hypothetical protein T8B10.250 - Arabido... 250 6e-67
TC78363 similar to PIR|D86405|D86405 unknown protein [imported] ... 40 0.002
TC87512 similar to PIR|F96607|F96607 probable clathrin-associate... 30 1.8
BG647262 similar to GP|9758917|dbj| gene_id:MXI22.6~unknown prot... 28 3.9
TC77808 similar to PIR|T03843|T03843 prohibitin - common tobacco... 28 5.2
BQ136714 similar to GP|22795875|emb maturase {Boopis graminea}, ... 27 8.8
>AW683633 PIR|T47870|T47 hypothetical protein T8B10.250 - Arabidopsis
thaliana, partial (4%)
Length = 633
Score = 250 bits (638), Expect = 6e-67
Identities = 133/172 (77%), Positives = 138/172 (79%), Gaps = 4/172 (2%)
Frame = +2
Query: 1 MASSLIHTHYKLHTSTFERAYPRSHGLLKSGKPFQSRRFNFPSIRLNQSFICCTKLTPWE 60
MASSLIHTHYKLHTSTFERAYPRSHGLLKSGKPFQSRRFNFPSIRLNQSFICCTKLTPWE
Sbjct: 125 MASSLIHTHYKLHTSTFERAYPRSHGLLKSGKPFQSRRFNFPSIRLNQSFICCTKLTPWE 304
Query: 61 PSPGVAYAPTDNQSDNFLQSTASVFETLESSKVDESPTANVEGLVEEKDRPGPELQLFK- 119
PSPGVAYAPTDNQSDNFLQSTASVFETLESSKVDESPTANVEGLVEEKDRPGP F+
Sbjct: 305 PSPGVAYAPTDNQSDNFLQSTASVFETLESSKVDESPTANVEGLVEEKDRPGPRASAFQV 484
Query: 120 ---WPMWLLGPSILLATGMVPTLWLPISSIFLGPNIASLLSLIGLDCIFNLG 168
P T ++ T S + P +LLSLIGLDCIFNLG
Sbjct: 485 ANVASRPFHSPCDRHGTNIMVTDIFNFSRSQI*P---ALLSLIGLDCIFNLG 631
>TC78363 similar to PIR|D86405|D86405 unknown protein [imported] -
Arabidopsis thaliana, partial (82%)
Length = 1105
Score = 39.7 bits (91), Expect = 0.002
Identities = 45/171 (26%), Positives = 72/171 (41%), Gaps = 12/171 (7%)
Frame = +1
Query: 161 LDCIFNLGATLFLLMADSCSRPKNPTQEIKSKAPFSYQ------FWNIVATLTGFIVPSL 214
LD +F A+ ++L + P +P+ S+ +Q + IV T G +P
Sbjct: 298 LDLVFPALASAYILALARYAFPSSPSTTSNSQQQEIFQVSSMFRMYIIVGTTIGLFLPLA 477
Query: 215 LMFGSQKGFLQPQLPFISSAVLLGPYLLLLSVQILTELL---TWYWQSPVWLVTPIIYEA 271
+ G GF + + SA P+L LLS QILTE + + PV + P+IY
Sbjct: 478 YVLG---GFARGDQHAVRSAT---PHLFLLSFQILTENIISSLSLFSPPVRALVPMIYTI 639
Query: 272 YRI---LQLMRGLKLGAELTAPAWMMHTIRGLVCWWVLILGLQLMRVAWFA 319
RI + + + L A A T W+ GL + +A+F+
Sbjct: 640 RRIFVDIDWINDVWLNKTFPANARFQDT-----AWYWFGKGLAVANLAYFS 777
>TC87512 similar to PIR|F96607|F96607 probable clathrin-associated adaptor
protein F25P12.96 [imported] - Arabidopsis thaliana,
partial (97%)
Length = 1620
Score = 29.6 bits (65), Expect = 1.8
Identities = 14/44 (31%), Positives = 22/44 (49%)
Frame = +1
Query: 30 SGKPFQSRRFNFPSIRLNQSFICCTKLTPWEPSPGVAYAPTDNQ 73
+G P + F PSI + F C + PWE + +++ P D Q
Sbjct: 772 TGLPDLTLSFTNPSILDDVRFHPCVRFRPWESNQILSFVPPDGQ 903
>BG647262 similar to GP|9758917|dbj| gene_id:MXI22.6~unknown protein
{Arabidopsis thaliana}, partial (6%)
Length = 782
Score = 28.5 bits (62), Expect = 3.9
Identities = 13/46 (28%), Positives = 24/46 (51%)
Frame = -2
Query: 163 CIFNLGATLFLLMADSCSRPKNPTQEIKSKAPFSYQFWNIVATLTG 208
CIFN+ +F + S+P + + + P S++FW +A +G
Sbjct: 583 CIFNI*NKIFNISLSLLSQPHRHSASVITFLPGSWRFW*RIALFSG 446
>TC77808 similar to PIR|T03843|T03843 prohibitin - common tobacco, partial
(96%)
Length = 1202
Score = 28.1 bits (61), Expect = 5.2
Identities = 21/75 (28%), Positives = 33/75 (44%)
Frame = +1
Query: 57 TPWEPSPGVAYAPTDNQSDNFLQSTASVFETLESSKVDESPTANVEGLVEEKDRPGPELQ 116
TP VA AP+ SV E + SS +S T+++ L P P +
Sbjct: 154 TPLSTPSTVANAPSSLTDSAASFPNQSVKELISSSHGFKSLTSSISALDLTHSHPSPVPK 333
Query: 117 LFKWPMWLLGPSILL 131
+F+W + L S++L
Sbjct: 334 IFRW*ISLFVFSLVL 378
>BQ136714 similar to GP|22795875|emb maturase {Boopis graminea}, partial (4%)
Length = 1268
Score = 27.3 bits (59), Expect = 8.8
Identities = 10/23 (43%), Positives = 13/23 (56%)
Frame = -2
Query: 103 GLVEEKDRPGPELQLFKWPMWLL 125
GL ++ RP P L+ WP W L
Sbjct: 322 GLARDRARPPPPLRARAWPWWAL 254
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.322 0.137 0.430
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,559,040
Number of Sequences: 36976
Number of extensions: 201485
Number of successful extensions: 1187
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1185
length of query: 344
length of database: 9,014,727
effective HSP length: 97
effective length of query: 247
effective length of database: 5,428,055
effective search space: 1340729585
effective search space used: 1340729585
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 59 (27.3 bits)
Medicago: description of AC148718.5