Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC148718.5 + phase: 0 
         (344 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

AW683633 PIR|T47870|T47 hypothetical protein T8B10.250 - Arabido...   250  6e-67
TC78363 similar to PIR|D86405|D86405 unknown protein [imported] ...    40  0.002
TC87512 similar to PIR|F96607|F96607 probable clathrin-associate...    30  1.8
BG647262 similar to GP|9758917|dbj| gene_id:MXI22.6~unknown prot...    28  3.9
TC77808 similar to PIR|T03843|T03843 prohibitin - common tobacco...    28  5.2
BQ136714 similar to GP|22795875|emb maturase {Boopis graminea}, ...    27  8.8

>AW683633 PIR|T47870|T47 hypothetical protein T8B10.250 - Arabidopsis
           thaliana, partial (4%)
          Length = 633

 Score =  250 bits (638), Expect = 6e-67
 Identities = 133/172 (77%), Positives = 138/172 (79%), Gaps = 4/172 (2%)
 Frame = +2

Query: 1   MASSLIHTHYKLHTSTFERAYPRSHGLLKSGKPFQSRRFNFPSIRLNQSFICCTKLTPWE 60
           MASSLIHTHYKLHTSTFERAYPRSHGLLKSGKPFQSRRFNFPSIRLNQSFICCTKLTPWE
Sbjct: 125 MASSLIHTHYKLHTSTFERAYPRSHGLLKSGKPFQSRRFNFPSIRLNQSFICCTKLTPWE 304

Query: 61  PSPGVAYAPTDNQSDNFLQSTASVFETLESSKVDESPTANVEGLVEEKDRPGPELQLFK- 119
           PSPGVAYAPTDNQSDNFLQSTASVFETLESSKVDESPTANVEGLVEEKDRPGP    F+ 
Sbjct: 305 PSPGVAYAPTDNQSDNFLQSTASVFETLESSKVDESPTANVEGLVEEKDRPGPRASAFQV 484

Query: 120 ---WPMWLLGPSILLATGMVPTLWLPISSIFLGPNIASLLSLIGLDCIFNLG 168
                     P     T ++ T     S   + P   +LLSLIGLDCIFNLG
Sbjct: 485 ANVASRPFHSPCDRHGTNIMVTDIFNFSRSQI*P---ALLSLIGLDCIFNLG 631


>TC78363 similar to PIR|D86405|D86405 unknown protein [imported] -
           Arabidopsis thaliana, partial (82%)
          Length = 1105

 Score = 39.7 bits (91), Expect = 0.002
 Identities = 45/171 (26%), Positives = 72/171 (41%), Gaps = 12/171 (7%)
 Frame = +1

Query: 161 LDCIFNLGATLFLLMADSCSRPKNPTQEIKSKAPFSYQ------FWNIVATLTGFIVPSL 214
           LD +F   A+ ++L     + P +P+    S+    +Q       + IV T  G  +P  
Sbjct: 298 LDLVFPALASAYILALARYAFPSSPSTTSNSQQQEIFQVSSMFRMYIIVGTTIGLFLPLA 477

Query: 215 LMFGSQKGFLQPQLPFISSAVLLGPYLLLLSVQILTELL---TWYWQSPVWLVTPIIYEA 271
            + G   GF +     + SA    P+L LLS QILTE +      +  PV  + P+IY  
Sbjct: 478 YVLG---GFARGDQHAVRSAT---PHLFLLSFQILTENIISSLSLFSPPVRALVPMIYTI 639

Query: 272 YRI---LQLMRGLKLGAELTAPAWMMHTIRGLVCWWVLILGLQLMRVAWFA 319
            RI   +  +  + L     A A    T      W+    GL +  +A+F+
Sbjct: 640 RRIFVDIDWINDVWLNKTFPANARFQDT-----AWYWFGKGLAVANLAYFS 777


>TC87512 similar to PIR|F96607|F96607 probable clathrin-associated adaptor
           protein F25P12.96 [imported] - Arabidopsis thaliana,
           partial (97%)
          Length = 1620

 Score = 29.6 bits (65), Expect = 1.8
 Identities = 14/44 (31%), Positives = 22/44 (49%)
 Frame = +1

Query: 30  SGKPFQSRRFNFPSIRLNQSFICCTKLTPWEPSPGVAYAPTDNQ 73
           +G P  +  F  PSI  +  F  C +  PWE +  +++ P D Q
Sbjct: 772 TGLPDLTLSFTNPSILDDVRFHPCVRFRPWESNQILSFVPPDGQ 903


>BG647262 similar to GP|9758917|dbj| gene_id:MXI22.6~unknown protein
           {Arabidopsis thaliana}, partial (6%)
          Length = 782

 Score = 28.5 bits (62), Expect = 3.9
 Identities = 13/46 (28%), Positives = 24/46 (51%)
 Frame = -2

Query: 163 CIFNLGATLFLLMADSCSRPKNPTQEIKSKAPFSYQFWNIVATLTG 208
           CIFN+   +F +     S+P   +  + +  P S++FW  +A  +G
Sbjct: 583 CIFNI*NKIFNISLSLLSQPHRHSASVITFLPGSWRFW*RIALFSG 446


>TC77808 similar to PIR|T03843|T03843 prohibitin - common tobacco, partial
           (96%)
          Length = 1202

 Score = 28.1 bits (61), Expect = 5.2
 Identities = 21/75 (28%), Positives = 33/75 (44%)
 Frame = +1

Query: 57  TPWEPSPGVAYAPTDNQSDNFLQSTASVFETLESSKVDESPTANVEGLVEEKDRPGPELQ 116
           TP      VA AP+            SV E + SS   +S T+++  L      P P  +
Sbjct: 154 TPLSTPSTVANAPSSLTDSAASFPNQSVKELISSSHGFKSLTSSISALDLTHSHPSPVPK 333

Query: 117 LFKWPMWLLGPSILL 131
           +F+W + L   S++L
Sbjct: 334 IFRW*ISLFVFSLVL 378


>BQ136714 similar to GP|22795875|emb maturase {Boopis graminea}, partial (4%)
          Length = 1268

 Score = 27.3 bits (59), Expect = 8.8
 Identities = 10/23 (43%), Positives = 13/23 (56%)
 Frame = -2

Query: 103 GLVEEKDRPGPELQLFKWPMWLL 125
           GL  ++ RP P L+   WP W L
Sbjct: 322 GLARDRARPPPPLRARAWPWWAL 254


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.322    0.137    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,559,040
Number of Sequences: 36976
Number of extensions: 201485
Number of successful extensions: 1187
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1185
length of query: 344
length of database: 9,014,727
effective HSP length: 97
effective length of query: 247
effective length of database: 5,428,055
effective search space: 1340729585
effective search space used: 1340729585
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 59 (27.3 bits)


Medicago: description of AC148718.5