BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148528.1 + phase: 0
(131 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AJ496888 similar to PIR|T47757|T477 hypothetical protein F24I3.5... 34 0.019
TC79250 similar to PIR|T06329|T06329 symbiotic ammonium transpor... 33 0.025
TC89598 weakly similar to GP|20127014|gb|AAM10934.1 putative bHL... 32 0.055
TC79851 similar to GP|18253965|gb|AAL66714.1 ABC transporter-lik... 32 0.055
TC90345 similar to GP|20127014|gb|AAM10934.1 putative bHLH trans... 31 0.16
BI310616 similar to GP|21593074|gb| bHLH DNA-binding protein-lik... 30 0.21
TC85987 homologue to GP|1370178|emb|CAA98166.1 RAB5A {Lotus japo... 30 0.27
TC92981 similar to PIR|T47758|T47758 hypothetical protein F24I3.... 30 0.36
BG452053 homologue to GP|11994233|db contains similarity to heli... 29 0.61
TC78811 similar to GP|3641870|emb|CAA09459.1 hypothetical protei... 28 0.80
TC82532 similar to PIR|F96745|F96745 unknown protein T9N14.4 [im... 28 1.0
BQ141772 28 1.0
TC78413 similar to PIR|T06329|T06329 symbiotic ammonium transpor... 28 1.4
TC83199 similar to GP|20466774|gb|AAM20704.1 putative protein {A... 28 1.4
TC81020 weakly similar to GP|15912327|gb|AAL08297.1 At1g25320/F4... 27 1.8
TC91064 weakly similar to GP|20127014|gb|AAM10934.1 putative bHL... 27 1.8
CA919209 similar to SP|O80536|PIF3_ Phytochrome-interacting fact... 27 2.3
TC88287 similar to PIR|T01364|T01364 homeodomain transcription f... 26 3.9
TC83351 similar to PIR|T47788|T47788 hypothetical protein F17J16... 26 5.2
TC77146 weakly similar to PIR|D84674|D84674 hypothetical protein... 26 5.2
>AJ496888 similar to PIR|T47757|T477 hypothetical protein F24I3.50 -
Arabidopsis thaliana, partial (13%)
Length = 676
Score = 33.9 bits (76), Expect = 0.019
Identities = 13/25 (52%), Positives = 20/25 (80%)
Frame = -3
Query: 92 MHILTERERRKKMRNMFDSLHALLP 116
+H +ER+RRKK+ N++ SL +LLP
Sbjct: 338 VHYASERDRRKKINNLYSSLRSLLP 264
>TC79250 similar to PIR|T06329|T06329 symbiotic ammonium transport protein
SAT1 - soybean, partial (34%)
Length = 1145
Score = 33.5 bits (75), Expect = 0.025
Identities = 18/47 (38%), Positives = 28/47 (59%)
Frame = +2
Query: 72 ENNITTGEEKDKKYRDFDHEMHILTERERRKKMRNMFDSLHALLPEL 118
+ NI ++ D R+ HI+ ER+RR+K+ F +L ALLP+L
Sbjct: 455 KRNIQESKKTDSAARNAQD--HIIAERKRREKISQKFIALSALLPDL 589
>TC89598 weakly similar to GP|20127014|gb|AAM10934.1 putative bHLH
transcription factor {Arabidopsis thaliana}, partial
(24%)
Length = 849
Score = 32.3 bits (72), Expect = 0.055
Identities = 19/71 (26%), Positives = 35/71 (48%), Gaps = 5/71 (7%)
Frame = +3
Query: 53 EAEAPVSKKRNWGGVV-----IRSENNITTGEEKDKKYRDFDHEMHILTERERRKKMRNM 107
+ E + N+G V+ + EN ++ + RD H++ ER+RR+K+
Sbjct: 420 KTEIGYGENLNFGSVISQGDYYKRENKVSAVNRNPMQARD-----HVIAERKRREKLSQR 584
Query: 108 FDSLHALLPEL 118
F +L ++LP L
Sbjct: 585 FIALSSILPGL 617
>TC79851 similar to GP|18253965|gb|AAL66714.1 ABC transporter-like protein
{Glycine max}, partial (35%)
Length = 721
Score = 32.3 bits (72), Expect = 0.055
Identities = 15/53 (28%), Positives = 29/53 (54%), Gaps = 2/53 (3%)
Frame = +3
Query: 44 PLNQNEGINEAEAPVSKKRNWGGVVI--RSENNITTGEEKDKKYRDFDHEMHI 94
P NEG+++ EAP K G ++ R + I + KD++ R+ +++H+
Sbjct: 327 PFRMNEGLDDGEAPKKKPEPVDGPLLSERDKLKIERRKRKDERQREAKYQLHL 485
>TC90345 similar to GP|20127014|gb|AAM10934.1 putative bHLH transcription
factor {Arabidopsis thaliana}, partial (21%)
Length = 906
Score = 30.8 bits (68), Expect = 0.16
Identities = 23/77 (29%), Positives = 33/77 (41%)
Frame = +1
Query: 42 TKPLNQNEGINEAEAPVSKKRNWGGVVIRSENNITTGEEKDKKYRDFDHEMHILTERERR 101
TK +N G + S K+N + TT D HI+ ER RR
Sbjct: 469 TKVVNSKNGPRRVKNNESNKKNGS-----FSKSTTTHHTPD----------HIIAERIRR 603
Query: 102 KKMRNMFDSLHALLPEL 118
+K+ +F +L AL+P L
Sbjct: 604 EKISQLFIALSALIPNL 654
>BI310616 similar to GP|21593074|gb| bHLH DNA-binding protein-like protein
{Arabidopsis thaliana}, partial (24%)
Length = 656
Score = 30.4 bits (67), Expect = 0.21
Identities = 24/83 (28%), Positives = 38/83 (44%)
Frame = +2
Query: 35 SSKKRVDTKPLNQNEGINEAEAPVSKKRNWGGVVIRSENNITTGEEKDKKYRDFDHEMHI 94
+S+KR + + Q E +N + KKR R + + K+K+ + HI
Sbjct: 311 NSRKR---QSMEQEETMNNPQVVQRKKR-------RRKPRVC----KNKEEAETQRITHI 448
Query: 95 LTERERRKKMRNMFDSLHALLPE 117
ER RRK+M L +L+PE
Sbjct: 449 TVERNRRKQMNEHLAVLRSLMPE 517
>TC85987 homologue to GP|1370178|emb|CAA98166.1 RAB5A {Lotus japonicus},
complete
Length = 1028
Score = 30.0 bits (66), Expect = 0.27
Identities = 27/76 (35%), Positives = 35/76 (45%), Gaps = 10/76 (13%)
Frame = -2
Query: 43 KPLNQNEGINEA---EAPVSKKRNWG---GVVIRSE----NNITTGEEKDKKYRDFDHEM 92
K L N+ N+A ++KK +G GV RS I EEK KK D + EM
Sbjct: 292 KELTLNKTQNKAGFSSTHITKKNQFGVEVGVSDRSH*VEIGTIGMEEEKRKKLLDEEIEM 113
Query: 93 HILTERERRKKMRNMF 108
I R RRK+ + F
Sbjct: 112 EICRRRRRRKQSLSCF 65
>TC92981 similar to PIR|T47758|T47758 hypothetical protein F24I3.60 -
Arabidopsis thaliana, partial (27%)
Length = 828
Score = 29.6 bits (65), Expect = 0.36
Identities = 12/24 (50%), Positives = 18/24 (75%)
Frame = +3
Query: 93 HILTERERRKKMRNMFDSLHALLP 116
H +ER+RRKK+ ++ SL +LLP
Sbjct: 228 HNASERDRRKKINSLISSLRSLLP 299
>BG452053 homologue to GP|11994233|db contains similarity to helix-loop-helix
DNA-binding protein~gene_id:MUJ8.4 {Arabidopsis
thaliana}, partial (27%)
Length = 613
Score = 28.9 bits (63), Expect = 0.61
Identities = 28/105 (26%), Positives = 43/105 (40%), Gaps = 15/105 (14%)
Frame = +1
Query: 27 NSDHNLGESSKK----RVDTKPLNQNEGINEAEAPVSKKRNWGGVVIRSENNITTGEEKD 82
+ D LGE+ ++ V+ K +Q + EA VS + N E N +E +
Sbjct: 85 SGDDGLGENDERFKLTSVEDKSRDQQD---HEEARVSDENN-----SVQEKNCAVVQENN 240
Query: 83 KKYR-----------DFDHEMHILTERERRKKMRNMFDSLHALLP 116
KK + + HI ER RRK+M L +L+P
Sbjct: 241 KKRKRPRTVKTSEEVESQRMTHIAVERNRRKQMNEHLRVLRSLMP 375
>TC78811 similar to GP|3641870|emb|CAA09459.1 hypothetical protein {Cicer
arietinum}, partial (90%)
Length = 1182
Score = 28.5 bits (62), Expect = 0.80
Identities = 13/37 (35%), Positives = 20/37 (53%)
Frame = +2
Query: 81 KDKKYRDFDHEMHILTERERRKKMRNMFDSLHALLPE 117
K+K+ + HI ER RRK+M L +L+P+
Sbjct: 410 KNKEEIENQRMTHIAVERNRRKQMNEYLSILRSLMPD 520
>TC82532 similar to PIR|F96745|F96745 unknown protein T9N14.4 [imported] -
Arabidopsis thaliana, partial (18%)
Length = 627
Score = 28.1 bits (61), Expect = 1.0
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +2
Query: 81 KDKKYRDFDHEMHILTERERRKKMRNMFDSLHALLP 116
K+K+ + HI ER RRK+M + L +L+P
Sbjct: 467 KNKEEIENQRMTHITVERNRRKQMNEYLNVLRSLMP 574
>BQ141772
Length = 751
Score = 28.1 bits (61), Expect = 1.0
Identities = 14/45 (31%), Positives = 20/45 (44%)
Frame = +2
Query: 15 FIWENHLWGDLPNSDHNLGESSKKRVDTKPLNQNEGINEAEAPVS 59
F+ H W DLP DH L + R+ +Q I ++ P S
Sbjct: 275 FLKSRHGWKDLPLDDHTLVNDHRTRISCP--SQTRDIRDSHTPES 403
>TC78413 similar to PIR|T06329|T06329 symbiotic ammonium transport protein
SAT1 - soybean, partial (30%)
Length = 1397
Score = 27.7 bits (60), Expect = 1.4
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = +2
Query: 93 HILTERERRKKMRNMFDSLHALLPEL 118
HI+ ER+RR+K+ +L AL+P L
Sbjct: 533 HIIAERKRREKLSQCLIALAALIPGL 610
>TC83199 similar to GP|20466774|gb|AAM20704.1 putative protein {Arabidopsis
thaliana}, partial (18%)
Length = 748
Score = 27.7 bits (60), Expect = 1.4
Identities = 13/32 (40%), Positives = 18/32 (55%)
Frame = -2
Query: 50 GINEAEAPVSKKRNWGGVVIRSENNITTGEEK 81
GI P K+RN ++R+E ITTG +K
Sbjct: 672 GIIPGSLPEVKRRNRKSRIVRTEPTITTGHQK 577
>TC81020 weakly similar to GP|15912327|gb|AAL08297.1 At1g25320/F4F7_17
{Arabidopsis thaliana}, partial (36%)
Length = 1024
Score = 27.3 bits (59), Expect = 1.8
Identities = 21/68 (30%), Positives = 33/68 (47%)
Frame = +1
Query: 9 QGVQTGFIWENHLWGDLPNSDHNLGESSKKRVDTKPLNQNEGINEAEAPVSKKRNWGGVV 68
QG+Q+ ++ N G +PN HNL + + T L+QN A + + + +V
Sbjct: 529 QGLQSLVLYGNSFSGSVPNEIHNL-----RYLQTLDLSQNFFNGSFPASIVQCKRLKTLV 693
Query: 69 IRSENNIT 76
I S NN T
Sbjct: 694 I-SRNNFT 714
>TC91064 weakly similar to GP|20127014|gb|AAM10934.1 putative bHLH
transcription factor {Arabidopsis thaliana}, partial
(19%)
Length = 751
Score = 27.3 bits (59), Expect = 1.8
Identities = 15/48 (31%), Positives = 22/48 (45%)
Frame = +2
Query: 71 SENNITTGEEKDKKYRDFDHEMHILTERERRKKMRNMFDSLHALLPEL 118
S + G K D HI++ER RR+ + + L AL+P L
Sbjct: 116 SSSTANQGSNSKKPRNTSDTLDHIMSERNRRQLLTSKIIELSALIPGL 259
>CA919209 similar to SP|O80536|PIF3_ Phytochrome-interacting factor 3
(Phytochrome-associated protein 3), partial (11%)
Length = 727
Score = 26.9 bits (58), Expect = 2.3
Identities = 14/40 (35%), Positives = 23/40 (57%)
Frame = -2
Query: 91 EMHILTERERRKKMRNMFDSLHALLPELPSKVNSFSSSIS 130
E+H L+ER RR ++ +L L+P +KV+ F S +
Sbjct: 348 EVHNLSERRRRDRINEKMRALQELIPNC-NKVSFFPSDFA 232
>TC88287 similar to PIR|T01364|T01364 homeodomain transcription factor
(ATHB-14) [imported] - Arabidopsis thaliana, partial
(33%)
Length = 1344
Score = 26.2 bits (56), Expect = 3.9
Identities = 12/41 (29%), Positives = 23/41 (55%), Gaps = 1/41 (2%)
Frame = -2
Query: 90 HEMHILTERER-RKKMRNMFDSLHALLPELPSKVNSFSSSI 129
H+ H ++ ++++ +HA L ELPS N F++S+
Sbjct: 248 HQFHCKAAKDTYHVPVQHLLGRMHAPLYELPSSTNPFTASL 126
>TC83351 similar to PIR|T47788|T47788 hypothetical protein F17J16.110 -
Arabidopsis thaliana, partial (18%)
Length = 882
Score = 25.8 bits (55), Expect = 5.2
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +1
Query: 77 TGEEKDKKYRDFDHEMHILTERERRKKMRNMFDSLHALLP 116
T + R+ E+H L+ER RR ++ +L L+P
Sbjct: 40 TSQRSGSARRNRAAEVHNLSERRRRDRINEKMKALQQLIP 159
>TC77146 weakly similar to PIR|D84674|D84674 hypothetical protein At2g27580
[imported] - Arabidopsis thaliana, partial (74%)
Length = 956
Score = 25.8 bits (55), Expect = 5.2
Identities = 25/76 (32%), Positives = 34/76 (43%), Gaps = 2/76 (2%)
Frame = -1
Query: 30 HNLGESSKKRVDTKPLNQNEGINEAEAPVSKKRNWGGVVIRSENNITTGEEKDKKYRDFD 89
H G S KR+ L + E E SK + ENN+ GEEK ++ +
Sbjct: 263 HVRGGSKSKRILFYLLGFSFSFKEEEKGKSKNIRRRR---KRENNMRCGEEK----KEMN 105
Query: 90 HEM--HILTERERRKK 103
EM ++L ERRKK
Sbjct: 104 EEMNWNVLIIEERRKK 57
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.310 0.129 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,956,816
Number of Sequences: 36976
Number of extensions: 44392
Number of successful extensions: 226
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 226
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 226
length of query: 131
length of database: 9,014,727
effective HSP length: 85
effective length of query: 46
effective length of database: 5,871,767
effective search space: 270101282
effective search space used: 270101282
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 53 (25.0 bits)
Medicago: description of AC148528.1