Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC147472.6 + phase: 0 /pseudo
         (180 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC85336 similar to PIR|E86154|E86154 hypothetical protein AAG008...   303  2e-83
BM814717 similar to SP|Q55717|Y639_ Hypothetical protein slr0639...    31  0.30
AW687280 similar to PIR|C96760|C96 probable sucrose synthase T9L...    28  2.0
TC86007 similar to GP|3204101|emb|CAA07227.1 hypothetical protei...    27  5.7
TC80583 squalene monooxygenase 1 [Medicago truncatula]                 27  5.7
BQ137743                                                               26  7.5
TC82345 weakly similar to GP|19698867|gb|AAL91169.1 unknown prot...    26  9.8
BQ141916 similar to GP|19172379|gb| mannitol transporter {Apium ...    26  9.8

>TC85336 similar to PIR|E86154|E86154 hypothetical protein AAG00893.1
           [imported] - Arabidopsis thaliana, partial (82%)
          Length = 1296

 Score =  303 bits (777), Expect = 2e-83
 Identities = 151/173 (87%), Positives = 157/173 (90%)
 Frame = +3

Query: 2   MRMCLTGLAFGMGVLTIFASPPLYRTFCQATSYDGTVTRRERVEEKIARHDSNQTVTSRE 61
           M M LTGL FGM  L+ +A+ PLYR FCQAT Y GTVTRRE VEEKIARHDSNQTVTSRE
Sbjct: 780 MLMYLTGLVFGMVGLS-YAAVPLYRRFCQATGYGGTVTRRESVEEKIARHDSNQTVTSRE 956

Query: 62  IVVQFNADISDGMPWKFLPTQREVRVKPGESALAFYTVENQSSTPITGVSTYNVTPMKLG 121
           IVVQFNADI+DGM WKF+PTQREVRVKPGESALAFYT ENQSSTPITGVSTYNVTPMK  
Sbjct: 957 IVVQFNADIADGMQWKFVPTQREVRVKPGESALAFYTAENQSSTPITGVSTYNVTPMKAA 1136

Query: 122 VYFNKIQCFCFEEQRLLPGEKIDMPVFFYIDPEIEDDPKMNGINNIILSYTFF 174
           VYFNKIQCFCFEEQRLLPGEKIDMPVFFYIDPEIEDDPKM+GINNIILSYT F
Sbjct: 1137VYFNKIQCFCFEEQRLLPGEKIDMPVFFYIDPEIEDDPKMDGINNIILSYTLF 1295


>BM814717 similar to SP|Q55717|Y639_ Hypothetical protein slr0639. [strain
           PCC 6803] {Synechocystis sp.}, partial (8%)
          Length = 584

 Score = 30.8 bits (68), Expect = 0.30
 Identities = 23/80 (28%), Positives = 38/80 (46%), Gaps = 1/80 (1%)
 Frame = +3

Query: 38  VTRRERV-EEKIARHDSNQTVTSREIVVQFNADISDGMPWKFLPTQREVRVKPGESALAF 96
           +T+   V   KI R  +  T T++  +++ +    D       PT + +      +AL  
Sbjct: 324 ITKNNNVPRSKIRRRSTTWTDTTKMGIIKQSKTTEDNNI-NIRPTTKTIPTSSSSAAL-- 494

Query: 97  YTVENQSSTPITGVSTYNVT 116
           Y  +N SS PIT +S +NVT
Sbjct: 495 YMQQNISSMPITTISEHNVT 554


>AW687280 similar to PIR|C96760|C96 probable sucrose synthase T9L24.42
           [imported] - Arabidopsis thaliana, partial (15%)
          Length = 620

 Score = 28.1 bits (61), Expect = 2.0
 Identities = 12/45 (26%), Positives = 23/45 (50%)
 Frame = -3

Query: 94  LAFYTVENQSSTPITGVSTYNVTPMKLGVYFNKIQCFCFEEQRLL 138
           L F+T+ + +   I G    +  P+  G+Y   +  FCF + ++L
Sbjct: 207 LMFFTLSSLAIEKIIGFFLSSKNPLDEGIYLAMVFLFCFSKHQVL 73


>TC86007 similar to GP|3204101|emb|CAA07227.1 hypothetical protein {Cicer
            arietinum}, partial (72%)
          Length = 1440

 Score = 26.6 bits (57), Expect = 5.7
 Identities = 9/18 (50%), Positives = 14/18 (77%)
 Frame = +3

Query: 112  TYNVTPMKLGVYFNKIQC 129
            +YN TP+  GVYF +++C
Sbjct: 1032 SYNFTPLLRGVYF*RVKC 1085


>TC80583 squalene monooxygenase 1 [Medicago truncatula]
          Length = 1830

 Score = 26.6 bits (57), Expect = 5.7
 Identities = 11/20 (55%), Positives = 13/20 (65%)
 Frame = +1

Query: 120  LGVYFNKIQCFCFEEQRLLP 139
            LGVYF K Q  CF++  L P
Sbjct: 1405 LGVYFQKDQSLCFQD*TLAP 1464


>BQ137743 
          Length = 1153

 Score = 26.2 bits (56), Expect = 7.5
 Identities = 14/39 (35%), Positives = 19/39 (47%)
 Frame = -1

Query: 82   QREVRVKPGESALAFYTVENQSSTPITGVSTYNVTPMKL 120
            QRE++  P +    F+   N S  P  GVS   V  +KL
Sbjct: 1150 QREIKFYPKKRITPFFQGNNISKHPPPGVSKRKVLSLKL 1034


>TC82345 weakly similar to GP|19698867|gb|AAL91169.1 unknown protein
           {Arabidopsis thaliana}, partial (27%)
          Length = 1237

 Score = 25.8 bits (55), Expect = 9.8
 Identities = 18/55 (32%), Positives = 25/55 (44%)
 Frame = +1

Query: 37  TVTRRERVEEKIARHDSNQTVTSREIVVQFNADISDGMPWKFLPTQREVRVKPGE 91
           T+  RE  E++++   SN+   +   V  F  D SDG  WK      E  V  GE
Sbjct: 454 TLILREIPEDEVSNFLSNKDCLAACDVAAFVHDSSDGYSWKRSIDLLEKVVNQGE 618


>BQ141916 similar to GP|19172379|gb| mannitol transporter {Apium graveolens
           var. dulce}, partial (9%)
          Length = 824

 Score = 25.8 bits (55), Expect = 9.8
 Identities = 10/18 (55%), Positives = 13/18 (71%), Gaps = 1/18 (5%)
 Frame = +3

Query: 19  FASP-PLYRTFCQATSYD 35
           + SP P+YRT+C   SYD
Sbjct: 729 YTSPYPIYRTYCSRLSYD 782


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.322    0.138    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,407,532
Number of Sequences: 36976
Number of extensions: 65191
Number of successful extensions: 334
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 333
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 333
length of query: 180
length of database: 9,014,727
effective HSP length: 90
effective length of query: 90
effective length of database: 5,686,887
effective search space: 511819830
effective search space used: 511819830
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)


Medicago: description of AC147472.6