BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146308.4 + phase: 0
(357 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC83629 weakly similar to GP|6692261|gb|AAF24611.1| putative RNa... 109 2e-24
TC92306 67 8e-12
CB065285 weakly similar to PIR|A84500|A84 probable retroelement ... 65 4e-11
BF632316 57 8e-09
TC83574 38 0.007
TC83226 weakly similar to PIR|G86419|G86419 probable reverse tra... 31 0.64
TC76351 similar to GP|7635494|emb|CAB88666.1 putative UDP-glycos... 29 3.2
TC88747 similar to PIR|G71442|G71442 hypothetical protein - Arab... 28 5.4
BQ150379 28 5.4
TC84396 similar to GP|9294345|dbj|BAB02242.1 organic anion trans... 28 5.4
BQ122106 similar to GP|9366656|emb|C probable similar to ring-h2... 28 7.1
TC80062 weakly similar to GP|4115534|dbj|BAA36410.1 UDP-glycose:... 28 7.1
TC87619 similar to GP|21554673|gb|AAM63653.1 unknown {Arabidopsi... 28 7.1
AJ497987 weakly similar to GP|9927273|dbj Similar to Arabidopsis... 28 7.1
TC80088 similar to GP|9759352|dbj|BAB10007.1 gene_id:MAH20.14~un... 27 9.3
TC80061 weakly similar to GP|4115534|dbj|BAA36410.1 UDP-glycose:... 27 9.3
TC85623 similar to GP|13605696|gb|AAK32841.1 At2g45220/F4L23.27 ... 27 9.3
BE248994 similar to GP|16604364|gb| AT5g39570/MIJ24_40 {Arabidop... 27 9.3
AA660759 similar to GP|16604364|gb| AT5g39570/MIJ24_40 {Arabidop... 27 9.3
>TC83629 weakly similar to GP|6692261|gb|AAF24611.1| putative RNase H
{Arabidopsis thaliana}, partial (21%)
Length = 1071
Score = 109 bits (272), Expect = 2e-24
Identities = 58/121 (47%), Positives = 80/121 (65%)
Frame = +2
Query: 214 NPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHALKKGFTSICIQGDS 273
N G AGAGA+L ++DG+L+Y R+G+G T AEYRA++LG++HA KGF + +GDS
Sbjct: 587 NRGPAGAGALLLAEDGSLLYGFRQGLGHQTKESAEYRALLLGLKHASMKGFKYVTAKGDS 766
Query: 274 KLVCMQMKQIDGLWKVKNENLSTLYKVAKELKDKFVSFKISHVLRDLNSEADAQANLAVN 333
+LV + QI WK+K+E+L L A EL D F SF+I H+ R+ N A AN A+N
Sbjct: 767 ELV---INQILDPWKIKDEHLKKLCAEALELSDNFHSFRIQHISRERNYGAVRNANRAIN 937
Query: 334 L 334
L
Sbjct: 938 L 940
>TC92306
Length = 521
Score = 67.4 bits (163), Expect = 8e-12
Identities = 39/86 (45%), Positives = 47/86 (54%), Gaps = 1/86 (1%)
Frame = +2
Query: 253 ILGMRHALKKGFTSICIQGDSKLVCMQMKQIDGLWKVKNENLSTLYKVAKELKDKFVSFK 312
ILG+ A +G+ + ++GD + VC KQ G WKV N NL L A ELK F S
Sbjct: 2 ILGLNEARNQGYEHVHVRGDFQXVC---KQFXGSWKVNNPNLRNLCNXAVELKSNFKSVS 172
Query: 313 ISHVLRDLNSEADAQANLAVNL-AGE 337
+ HV R N ADAQAN N AGE
Sbjct: 173 VEHVPRGXNXAADAQANRGKNXGAGE 250
>CB065285 weakly similar to PIR|A84500|A84 probable retroelement gag/pol
polyprotein [imported] - Arabidopsis thaliana, partial
(2%)
Length = 592
Score = 65.1 bits (157), Expect = 4e-11
Identities = 45/125 (36%), Positives = 61/125 (48%)
Frame = -2
Query: 207 FDGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHALKKGFTS 266
FDGA N G GA++ S G+ I + TNN+AEY A I G+ A+
Sbjct: 522 FDGAV--NAYGKGIGAVIVSPQGHYIPFTARILFECTNNMAEYEACIFGIEEAIDMRIKH 349
Query: 267 ICIQGDSKLVCMQMKQIDGLWKVKNENLSTLYKVAKELKDKFVSFKISHVLRDLNSEADA 326
+ I GDS LV + QI G W+ + NL A+ L F ++ H+ RD N ADA
Sbjct: 348 LDIYGDSALV---INQIKGEWETHHANLIPYRDYARRLLTYFTKVELHHIPRDENQMADA 178
Query: 327 QANLA 331
A L+
Sbjct: 177 LATLS 163
>BF632316
Length = 370
Score = 57.4 bits (137), Expect = 8e-09
Identities = 26/47 (55%), Positives = 33/47 (69%)
Frame = +3
Query: 126 SVYKGYSMSNETEEYLLSHGLKNALYTIRASDLKEDLFGTLVPCPFQ 172
SV+KG+S+ ETEEYL+SHGLK A Y+I A+ + FG L CP Q
Sbjct: 57 SVFKGFSLPQETEEYLVSHGLKGATYSISAAHVNAGSFGRLAVCPHQ 197
>TC83574
Length = 374
Score = 37.7 bits (86), Expect = 0.007
Identities = 16/28 (57%), Positives = 20/28 (71%)
Frame = +3
Query: 74 KLHKVEAETTMNQEQNESDAFYVVRKGD 101
KL K + ET M+ ++NE D FYV RKGD
Sbjct: 291 KLXKTKXETRMDXQENERDGFYVXRKGD 374
>TC83226 weakly similar to PIR|G86419|G86419 probable reverse transcriptase
100033-105622 [imported] - Arabidopsis thaliana, partial
(2%)
Length = 885
Score = 31.2 bits (69), Expect = 0.64
Identities = 23/73 (31%), Positives = 36/73 (48%), Gaps = 1/73 (1%)
Frame = +3
Query: 210 ASKGNPGKAGAGAILRSKDGNLIYRVR-EGVGIATNNVAEYRAMILGMRHALKKGFTSIC 268
A+ N GK G G I+R + G ++ E G AE A++ GMR A GF +
Sbjct: 666 ANLQNHGKWGLGIIIRDEVGLVMAASTWETDGNDRALEAEAYALLTGMRFAKDCGFXKVX 845
Query: 269 IQGDSKLVCMQMK 281
+GD++ + +K
Sbjct: 846 FEGDNEKLMKMVK 884
>TC76351 similar to GP|7635494|emb|CAB88666.1 putative UDP-glycose {Cicer
arietinum}, partial (92%)
Length = 1645
Score = 28.9 bits (63), Expect = 3.2
Identities = 16/43 (37%), Positives = 21/43 (48%)
Frame = -1
Query: 5 FSHLSSFTSTILARTTQFVANRSLHTTPHTFSFPVKSHHSLLT 47
FS+ S+FT TI H H+F +P SHH LL+
Sbjct: 1399 FSYFSTFTITI-------------HQFLHSFLYPFSSHHCLLS 1310
>TC88747 similar to PIR|G71442|G71442 hypothetical protein - Arabidopsis
thaliana, partial (12%)
Length = 902
Score = 28.1 bits (61), Expect = 5.4
Identities = 25/66 (37%), Positives = 34/66 (50%), Gaps = 3/66 (4%)
Frame = +1
Query: 38 PVKSHHSLLTSFHSQ-FVL--TTVTRCYSTRKLRKTGSSKLHKVEAETTMNQEQNESDAF 94
PV S S++ H FVL TT+ S +L SSKL + + M +++NESD F
Sbjct: 313 PVLSFFSIIFFHHIFCFVLLNTTLLSPISHSQLLTFSSSKLQEFVSG**MMEQRNESDMF 492
Query: 95 YVVRKG 100
RKG
Sbjct: 493 QHWRKG 510
>BQ150379
Length = 874
Score = 28.1 bits (61), Expect = 5.4
Identities = 17/59 (28%), Positives = 27/59 (44%), Gaps = 4/59 (6%)
Frame = +2
Query: 8 LSSFTSTILARTTQFVANRSLHTTPHTFSFPVKSHHSLLTSFH----SQFVLTTVTRCY 62
+ S T+ TQ+ NRS H PH + V S+HS + + + F+ + CY
Sbjct: 422 IMSITARPYGNPTQY--NRSKHERPHLIFYIV*SYHSCIMIYTI*YITMFIYVYTSSCY 592
>TC84396 similar to GP|9294345|dbj|BAB02242.1 organic anion transporter-like
protein {Arabidopsis thaliana}, partial (22%)
Length = 564
Score = 28.1 bits (61), Expect = 5.4
Identities = 18/47 (38%), Positives = 27/47 (57%), Gaps = 6/47 (12%)
Frame = -1
Query: 9 SSFTSTILARTTQFVANRSLHTTPHTFS-----FPV-KSHHSLLTSF 49
S+FTS L R +A+ S+ T HTF+ FP+ K+H+ + SF
Sbjct: 363 STFTSNPLPRRRCHIADTSIGGTLHTFNTLPIRFPISKNHNHSMKSF 223
>BQ122106 similar to GP|9366656|emb|C probable similar to ring-h2 finger
protein rha1a. {Trypanosoma brucei}, partial (18%)
Length = 693
Score = 27.7 bits (60), Expect = 7.1
Identities = 21/80 (26%), Positives = 32/80 (39%)
Frame = -2
Query: 198 NNVKTCIVEFDGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMR 257
+N I+ DG+ GNP G ++R N+ G N+ ++L
Sbjct: 560 DNNSCYILNVDGSCLGNP*PTGFNGLIR----NIAGLFNSGF---PGNITNTSDILLAEL 402
Query: 258 HALKKGFTSICIQGDSKLVC 277
HA+ +G I G S VC
Sbjct: 401 HAIFQGLRMISDMGISDFVC 342
>TC80062 weakly similar to GP|4115534|dbj|BAA36410.1 UDP-glycose:flavonoid
glycosyltransferase {Vigna mungo}, partial (42%)
Length = 1071
Score = 27.7 bits (60), Expect = 7.1
Identities = 12/44 (27%), Positives = 26/44 (58%), Gaps = 1/44 (2%)
Frame = -3
Query: 3 ALFSHLSSFTSTILARTTQFVANRSL-HTTPHTFSFPVKSHHSL 45
A F+ + S +L+ + F++ ++ H +PH+F + + +HH L
Sbjct: 622 AFFNSMCSLFPKLLSTLSDFLSFITIVHQSPHSFFYTMSTHHFL 491
>TC87619 similar to GP|21554673|gb|AAM63653.1 unknown {Arabidopsis
thaliana}, partial (33%)
Length = 892
Score = 27.7 bits (60), Expect = 7.1
Identities = 18/48 (37%), Positives = 22/48 (45%)
Frame = +1
Query: 8 LSSFTSTILARTTQFVANRSLHTTPHTFSFPVKSHHSLLTSFHSQFVL 55
LSS + RT Q VA H++PH + S LL SF F L
Sbjct: 22 LSSSHVWVSERTQQNVAKHRNHSSPHRQNHFTPSFFPLLISFTKHFFL 165
>AJ497987 weakly similar to GP|9927273|dbj Similar to Arabidopsis thaliana
chromosome II BAC F26H6; putative retroelement pol
polyprotein, partial (1%)
Length = 636
Score = 27.7 bits (60), Expect = 7.1
Identities = 21/52 (40%), Positives = 26/52 (49%), Gaps = 2/52 (3%)
Frame = -2
Query: 207 FDGASK--GNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGM 256
FDGA GN G GA+L + G I TNN+AEY A I+G+
Sbjct: 278 FDGAVNVYGN----GIGAVLLTPKGAHIPFTARLRFDCTNNIAEYEACIMGI 135
>TC80088 similar to GP|9759352|dbj|BAB10007.1 gene_id:MAH20.14~unknown
protein {Arabidopsis thaliana}, partial (73%)
Length = 1346
Score = 27.3 bits (59), Expect = 9.3
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = +1
Query: 14 TILARTTQFVANRSLHTTPHTFSFPVKSHHSLLTS 48
T++A + + SLH P + P S HSLL+S
Sbjct: 193 TVIAASNSVPTSHSLHPVPILLTNPSPSTHSLLSS 297
>TC80061 weakly similar to GP|4115534|dbj|BAA36410.1 UDP-glycose:flavonoid
glycosyltransferase {Vigna mungo}, partial (11%)
Length = 486
Score = 27.3 bits (59), Expect = 9.3
Identities = 11/37 (29%), Positives = 25/37 (66%), Gaps = 1/37 (2%)
Frame = -3
Query: 10 SFTSTILARTTQFVANRSL-HTTPHTFSFPVKSHHSL 45
S +S +L+ ++ F++ ++ H +PH+F + + +HH L
Sbjct: 133 SLSSKLLSTSSNFLSFITIVHQSPHSFFYTMSTHHFL 23
>TC85623 similar to GP|13605696|gb|AAK32841.1 At2g45220/F4L23.27 {Arabidopsis
thaliana}, partial (79%)
Length = 2442
Score = 27.3 bits (59), Expect = 9.3
Identities = 25/113 (22%), Positives = 46/113 (40%), Gaps = 2/113 (1%)
Frame = +3
Query: 145 GLKNALYTIRASDLKEDLFGTLV--PCPFQDPSSTQGTTSNADSSKKRALEVLEQNNVKT 202
G+ N + + ++++ + L TL P+Q PS G + ++ L+ +
Sbjct: 999 GVTNYVLPLLSNNVTKLLSNTLSLNKVPYQQPSYKDGFPTWVKPGDRKLLQTSSAASKAN 1178
Query: 203 CIVEFDGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILG 255
+V DG+ K KA A S G + V+ GV + M++G
Sbjct: 1179 VVVAKDGSGKYTTVKAATDA-APSGSGRYVIYVKAGVYNEQVEIKAKNVMLVG 1334
>BE248994 similar to GP|16604364|gb| AT5g39570/MIJ24_40 {Arabidopsis
thaliana}, partial (20%)
Length = 579
Score = 27.3 bits (59), Expect = 9.3
Identities = 10/22 (45%), Positives = 15/22 (67%)
Frame = +2
Query: 29 HTTPHTFSFPVKSHHSLLTSFH 50
H+ PH+FS ++H +L SFH
Sbjct: 230 HSPPHSFSHHNQTHIQILASFH 295
>AA660759 similar to GP|16604364|gb| AT5g39570/MIJ24_40 {Arabidopsis
thaliana}, partial (15%)
Length = 570
Score = 27.3 bits (59), Expect = 9.3
Identities = 10/22 (45%), Positives = 15/22 (67%)
Frame = -2
Query: 29 HTTPHTFSFPVKSHHSLLTSFH 50
H+ PH+FS ++H +L SFH
Sbjct: 194 HSPPHSFSHHNQTHIQILASFH 129
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.131 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,147,895
Number of Sequences: 36976
Number of extensions: 126828
Number of successful extensions: 850
Number of sequences better than 10.0: 39
Number of HSP's better than 10.0 without gapping: 846
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 847
length of query: 357
length of database: 9,014,727
effective HSP length: 97
effective length of query: 260
effective length of database: 5,428,055
effective search space: 1411294300
effective search space used: 1411294300
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146308.4