BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144617.5 + phase: 0
(428 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80236 similar to GP|8953395|emb|CAB96668.1 putative protein {A... 853 0.0
TC79960 similar to PIR|T52419|T52419 CTF2A protein [imported] - ... 39 0.005
BF632400 similar to GP|9857294|dbj CpABA1 {Vigna unguiculata}, p... 37 0.015
TC88941 similar to PIR|T52419|T52419 CTF2A protein [imported] - ... 32 0.61
TC77933 similar to PIR|F86324|F86324 protein F14D16.29 [imported... 31 0.80
AW256544 similar to GP|21553864|gb| unknown {Arabidopsis thalian... 30 1.4
CA921811 similar to GP|14194125|gb At1g29900/F1N18_6 {Arabidopsi... 30 1.8
TC88192 weakly similar to PIR|T05682|T05682 monooxygenase (EC 1.... 29 3.0
TC82522 similar to PIR|A96708|A96708 hypothetical protein T2E12.... 28 5.2
BG648549 similar to PIR|E85359|E853 hypothetical protein AT4g307... 28 6.8
TC81052 weakly similar to GP|10178211|dbj|BAB11635. TMV resistan... 23 7.6
TC84526 homologue to PIR|B96625|B96625 hypothetical protein T2K1... 28 8.9
TC90085 similar to GP|7767663|gb|AAF69160.1| F27F5.18 {Arabidops... 28 8.9
TC87740 similar to PIR|D84431|D84431 probable endosomal protein ... 28 8.9
>TC80236 similar to GP|8953395|emb|CAB96668.1 putative protein {Arabidopsis
thaliana}, partial (89%)
Length = 1378
Score = 853 bits (2205), Expect = 0.0
Identities = 422/425 (99%), Positives = 423/425 (99%)
Frame = +1
Query: 4 EGRKPKAVIVGGSIGGISSAHALILAGWDVLVLEKTTSPPTGSPTGAGLGLNSLSQQIIQ 63
EGRKPKAVIVGGSIGGISSAHALILAGWDVLVLEKTTSPPTGSPTGAGLGLNSLSQQIIQ
Sbjct: 1 EGRKPKAVIVGGSIGGISSAHALILAGWDVLVLEKTTSPPTGSPTGAGLGLNSLSQQIIQ 180
Query: 64 SWISKPPQFLHNTTHPLTIDQNLVTDSEKKVNRTLTRDESLNFRAAHWGDLHGVLYESLP 123
SWISKPPQFLHNTTHPLTIDQNLVTDSEKKVNRTLTRDESLNFRAAHWGDLHGVLYESLP
Sbjct: 181 SWISKPPQFLHNTTHPLTIDQNLVTDSEKKVNRTLTRDESLNFRAAHWGDLHGVLYESLP 360
Query: 124 PQIFLWGHIFLSFHVANEKGPVIVKAKVVKTGEVIEIVGDLLVAADGCLSSIRRKYLPDF 183
PQIFLWGHIFLSFHVANEKGPVIVKAKVVKTGEVIEIVGDLLVAADGCLSSIRRKYLPDF
Sbjct: 361 PQIFLWGHIFLSFHVANEKGPVIVKAKVVKTGEVIEIVGDLLVAADGCLSSIRRKYLPDF 540
Query: 184 KLRYSGYCAWRGVLDFSEIENSETIKGIKKAYPDLGKCLYFDLASGTHSVLYELPNKKLN 243
KLRYSGYCAWRGVLDFSEIENSETIKGIKKAYPDLGKCLYFDLASGTHSVLYELPNKKLN
Sbjct: 541 KLRYSGYCAWRGVLDFSEIENSETIKGIKKAYPDLGKCLYFDLASGTHSVLYELPNKKLN 720
Query: 244 WIWYVNQPEPEVKGTSVTTKVTSDMIQKMHQEAEKIWIPELAKVMKETKEPFLNFIYDSD 303
IWYVNQPEPE+KGTSVTTKVTSDMIQKMHQEAEKIWIPELAKVMKETK PFLNFIYDSD
Sbjct: 721 *IWYVNQPEPEMKGTSVTTKVTSDMIQKMHQEAEKIWIPELAKVMKETK*PFLNFIYDSD 900
Query: 304 PLEKIFWDNVVLVGEAAHPTTPHYLRSTNMTILDAAVLGKCIEKWGTEMLESALEEYQLT 363
PLEKIFWDNVVLVGEAAHPTTPHYLRSTNMTILDAAVLGKCIEKWGTEMLESALEEYQLT
Sbjct: 901 PLEKIFWDNVVLVGEAAHPTTPHYLRSTNMTILDAAVLGKCIEKWGTEMLESALEEYQLT 1080
Query: 364 RLPVTSKQVLHARRLGRIKQGLVLPDRDPFDPKSARQEECQEIILTNTPFFNDAPLSFAS 423
RLPVTSKQVLHARRLGRIKQGLVLPDRDPFDPKSARQEECQEIILTNTPFFNDAPLSFAS
Sbjct: 1081RLPVTSKQVLHARRLGRIKQGLVLPDRDPFDPKSARQEECQEIILTNTPFFNDAPLSFAS 1260
Query: 424 VTSSI 428
VTSSI
Sbjct: 1261VTSSI 1275
>TC79960 similar to PIR|T52419|T52419 CTF2A protein [imported] - Arabidopsis
thaliana, partial (53%)
Length = 907
Score = 38.5 bits (88), Expect = 0.005
Identities = 56/201 (27%), Positives = 88/201 (42%), Gaps = 5/201 (2%)
Frame = +1
Query: 6 RKPKAVIVGGSIGGISSAHALILAGWDVLVLEKTTSPPTG--SPTGAGLGLNSLSQQIIQ 63
RK VIVGG I G+++A +L G LVLE++ S TG S T + G ++L +
Sbjct: 187 RKEHVVIVGGGIAGLATALSLHRLGVRSLVLEQSESLRTGGTSLTLSKNGWSALDSIGVA 366
Query: 64 SWISKPPQFLHNTTHPLTIDQNLVTDSEKKVNRTLT---RDESLNFRAAHWGDLHGVLYE 120
+++ T L I ++ + K R L +D S RA VL E
Sbjct: 367 NYL---------RTQYLEIQGIVLKSEDGKELRALNFKEKDGSQELRAVE----RRVLLE 507
Query: 121 SLPPQIFLWGHIFLSFHVANEKGPVIVKAKVVKTGEVIEIVGDLLVAADGCLSSIRRKYL 180
+L Q+ + S V E P G +I+ +++ DG S I K++
Sbjct: 508 TLAGQLPTDTIQYSSRLVKIEPSPNGDTFLEFLDGS--KILAKIVIGCDGIRSPI-AKWM 678
Query: 181 PDFKLRYSGYCAWRGVLDFSE 201
+ Y GY A+RG+ +S+
Sbjct: 679 GFSEPNYVGYWAFRGLASYSD 741
>BF632400 similar to GP|9857294|dbj CpABA1 {Vigna unguiculata}, partial (21%)
Length = 391
Score = 37.0 bits (84), Expect = 0.015
Identities = 18/38 (47%), Positives = 21/38 (54%)
Frame = +1
Query: 162 GDLLVAADGCLSSIRRKYLPDFKLRYSGYCAWRGVLDF 199
GDLLV ADG S R K + YSGY + G+ DF
Sbjct: 241 GDLLVGADGIWSKGRTKLFGSTEATYSGYTCYTGIADF 354
>TC88941 similar to PIR|T52419|T52419 CTF2A protein [imported] - Arabidopsis
thaliana, partial (39%)
Length = 659
Score = 31.6 bits (70), Expect = 0.61
Identities = 38/122 (31%), Positives = 51/122 (41%), Gaps = 3/122 (2%)
Frame = +1
Query: 6 RKPKAVIVGGSIGGISSAHALILAGWDVLVLEKTTSPPTGSPTGAGLGLNSLSQQIIQSW 65
+K VIVGG I G+++A +L G LVLE++ S TG G L L ++ S
Sbjct: 139 QKEHVVIVGGGIAGLATALSLHRLGVRSLVLEQSESLRTG---GTSLTLFKNGWSVLDS- 306
Query: 66 ISKPPQFLHNTTHPLTIDQNLVTDSEKKVNRTLT---RDESLNFRAAHWGDLHGVLYESL 122
+L T L I +V + + R DES RA L L L
Sbjct: 307 -IGVANYLR--TQYLEIQGMVVKSEDGRELRAFNFKEEDESQEVRAVERRVLLETLAAQL 477
Query: 123 PP 124
PP
Sbjct: 478 PP 483
>TC77933 similar to PIR|F86324|F86324 protein F14D16.29 [imported] -
Arabidopsis thaliana, partial (57%)
Length = 857
Score = 31.2 bits (69), Expect = 0.80
Identities = 18/56 (32%), Positives = 29/56 (51%), Gaps = 9/56 (16%)
Frame = +2
Query: 221 CLYFDLASGTHSVLYELPNKKLNWIWYVNQP---------EPEVKGTSVTTKVTSD 267
C+YF L SGT S L+ +P+ + W Y+ +P + VK T + + +T D
Sbjct: 251 CIYFLLPSGTMSRLHRIPSSE-TWHHYIGEPITVVELNEKDSSVKFTCLGSDLTKD 415
>AW256544 similar to GP|21553864|gb| unknown {Arabidopsis thaliana}, partial
(42%)
Length = 628
Score = 30.4 bits (67), Expect = 1.4
Identities = 13/31 (41%), Positives = 20/31 (63%)
Frame = +2
Query: 221 CLYFDLASGTHSVLYELPNKKLNWIWYVNQP 251
C+YF L SGT S L+ +P+ + W Y+ +P
Sbjct: 170 CIYFLLPSGTMSRLHRIPSSE-TWHHYIGEP 259
>CA921811 similar to GP|14194125|gb At1g29900/F1N18_6 {Arabidopsis thaliana},
partial (3%)
Length = 798
Score = 30.0 bits (66), Expect = 1.8
Identities = 13/32 (40%), Positives = 20/32 (61%)
Frame = +1
Query: 114 LHGVLYESLPPQIFLWGHIFLSFHVANEKGPV 145
L GVLYE +++ ++F SF + NEK P+
Sbjct: 448 LSGVLYEESGQRLYDHSYLFWSFSLTNEKHPI 543
>TC88192 weakly similar to PIR|T05682|T05682 monooxygenase (EC 1.-.-.-) 2 -
Arabidopsis thaliana, partial (61%)
Length = 1557
Score = 29.3 bits (64), Expect = 3.0
Identities = 18/66 (27%), Positives = 30/66 (45%)
Frame = +3
Query: 312 NVVLVGEAAHPTTPHYLRSTNMTILDAAVLGKCIEKWGTEMLESALEEYQLTRLPVTSKQ 371
NV + G+A HP TP + + D VL +C+ + + + EE + R+ K+
Sbjct: 960 NVCVAGDALHPMTPDLGQGGCSALEDGVVLARCLAE---ALSKKQKEEEKYKRIEQGLKK 1130
Query: 372 VLHARR 377
RR
Sbjct: 1131 YAKERR 1148
Score = 28.9 bits (63), Expect = 4.0
Identities = 52/205 (25%), Positives = 83/205 (40%), Gaps = 6/205 (2%)
Frame = +2
Query: 11 VIVGGSIGGISSAHALILAGWDVLVLEKTTSPPTGSPTGAGLGLNSLSQQIIQS------ 64
VIVG I G+++A L G LVLE + S TG LG+ + + + +
Sbjct: 128 VIVGAGIAGLATALGLHRLGVRSLVLESSDSLRV---TGFALGIWENAWKALDAVGVGDI 298
Query: 65 WISKPPQFLHNTTHPLTIDQNLVTDSEKKVNRTLTRDESLNFRAAHWGDLHGVLYESLPP 124
K N T L Q L T S + + L R+ L L LP
Sbjct: 299 LRHKHIHLYGNVTTSLITGQQLATMSFRDKGK-------LELRSVRRKLLLEALGNELPN 457
Query: 125 QIFLWGHIFLSFHVANEKGPVIVKAKVVKTGEVIEIVGDLLVAADGCLSSIRRKYLPDFK 184
++S VA E+ K++ + I +L+ DG ++S+ K+L +
Sbjct: 458 STIR----YMSKVVAIEESGFF---KILHLVDGTTIKTKVLIGCDG-INSVVAKWLGFKE 613
Query: 185 LRYSGYCAWRGVLDFSEIENSETIK 209
Y+G RG F+E+EN+ + +
Sbjct: 614 ASYTGRYGIRG---FAELENNHSFE 679
>TC82522 similar to PIR|A96708|A96708 hypothetical protein T2E12.9
[imported] - Arabidopsis thaliana, partial (33%)
Length = 727
Score = 28.5 bits (62), Expect = 5.2
Identities = 14/52 (26%), Positives = 27/52 (51%)
Frame = -3
Query: 199 FSEIENSETIKGIKKAYPDLGKCLYFDLASGTHSVLYELPNKKLNWIWYVNQ 250
FS++ K +K+A+ + KCL+ +++ H+ PN +W VN+
Sbjct: 158 FSQVY*ISKSKTLKRAHTSIQKCLFHTVSTYLHTNSDFFPNLISENVWNVNK 3
>BG648549 similar to PIR|E85359|E853 hypothetical protein AT4g30720
[imported] - Arabidopsis thaliana, partial (7%)
Length = 736
Score = 28.1 bits (61), Expect = 6.8
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = +2
Query: 6 RKPKAVIVGGSIGGISSAHALILAGWDVLVLEK 38
RKPK IVG G+ +A L G DV ++E+
Sbjct: 626 RKPKIAIVGSGPSGLFAALVLAELGADVTLIER 724
>TC81052 weakly similar to GP|10178211|dbj|BAB11635. TMV resistance protein
N {Arabidopsis thaliana}, partial (8%)
Length = 656
Score = 23.1 bits (48), Expect(2) = 7.6
Identities = 10/28 (35%), Positives = 16/28 (56%)
Frame = +3
Query: 325 PHYLRSTNMTILDAAVLGKCIEKWGTEM 352
P+ + +NMT++ V GK KWG +
Sbjct: 450 PNMNKHSNMTLMWYKVGGKL*HKWGISL 533
Score = 23.1 bits (48), Expect(2) = 7.6
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +1
Query: 304 PLEKIFWDNVVLVGEAAHP 322
PL+K+F+ N+VL HP
Sbjct: 292 PLQKLFFLNLVLARIGTHP 348
>TC84526 homologue to PIR|B96625|B96625 hypothetical protein T2K10.12
[imported] - Arabidopsis thaliana, partial (17%)
Length = 664
Score = 27.7 bits (60), Expect = 8.9
Identities = 18/73 (24%), Positives = 32/73 (43%)
Frame = -2
Query: 321 HPTTPHYLRSTNMTILDAAVLGKCIEKWGTEMLESALEEYQLTRLPVTSKQVLHARRLGR 380
H P + +M I + + +C+ W +E+ + + P +S VLHA R+ R
Sbjct: 429 HSI*PKCVGYPSMWINISLAILRCL*SWSFSFIEARMAAHSFLTTPRSSAAVLHA-RMAR 253
Query: 381 IKQGLVLPDRDPF 393
I +P+ F
Sbjct: 252 IMSLKRVPEEKGF 214
>TC90085 similar to GP|7767663|gb|AAF69160.1| F27F5.18 {Arabidopsis
thaliana}, partial (29%)
Length = 551
Score = 27.7 bits (60), Expect = 8.9
Identities = 34/136 (25%), Positives = 49/136 (36%), Gaps = 20/136 (14%)
Frame = +3
Query: 48 TGAGLGLNSLSQQIIQSWISKPPQF-----LHNTTHPLTIDQNLVTDSEKK--------- 93
T A L L S S I + P F LH T HP TD+ +
Sbjct: 45 TAAPLPLYSFSSSIPKQLTFSPLSFCSTSELHLTQHPDNNKPEHETDNRRSQLLKPGLYL 224
Query: 94 VNRTLTRDESLNFRAAH-WGDLHGVLYESLPPQIFLWGHI-----FLSFHVANEKGPVIV 147
V + E + FRA H +L E L H +S+H NE +
Sbjct: 225 VGTPIGNLEDITFRALRVLNSAHVILSEDTRHSGKLLHHYNIKTPLMSYHKFNESQREQL 404
Query: 148 KAKVVKTGEVIEIVGD 163
+ +K GE++ ++ D
Sbjct: 405 VLRRLKQGEIVALISD 452
>TC87740 similar to PIR|D84431|D84431 probable endosomal protein [imported]
- Arabidopsis thaliana, partial (39%)
Length = 989
Score = 27.7 bits (60), Expect = 8.9
Identities = 17/45 (37%), Positives = 25/45 (54%), Gaps = 1/45 (2%)
Frame = +2
Query: 89 DSEKKVNRTLTRDESLNFRAAHWGDLH-GVLYESLPPQIFLWGHI 132
DS +TLT++E NFR+A D + + Y+ LP +WG I
Sbjct: 473 DSLSVCKKTLTKEEVANFRSAVRKDYYFQMYYDDLP----IWGFI 595
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.136 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,747,929
Number of Sequences: 36976
Number of extensions: 191810
Number of successful extensions: 954
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 950
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 954
length of query: 428
length of database: 9,014,727
effective HSP length: 99
effective length of query: 329
effective length of database: 5,354,103
effective search space: 1761499887
effective search space used: 1761499887
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC144617.5