
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC141109.13 - phase: 0
(208 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC89284 similar to GP|1890154|emb|CAA72432.1 alpha-mannosidase p... 40 5e-04
TC82621 similar to GP|10177664|dbj|BAB11126. alpha-mannosidase {... 39 0.001
BI309315 similar to GP|10177664|db alpha-mannosidase {Arabidopsi... 35 0.016
TC84130 similar to GP|14326479|gb|AAK60285.1 AT5g56980/MHM17_10 ... 30 0.66
TC80530 weakly similar to PIR|S44261|S44261 SRG1 protein - Arabi... 30 0.86
TC83679 weakly similar to PIR|G75403|G75403 DNA topoisomerase I ... 30 0.86
TC77254 similar to GP|10334497|emb|CAC10210. hypothetical protei... 29 1.1
TC84571 weakly similar to SP|P29136|MEP1_SOYBN Metalloendoprotei... 29 1.1
TC87274 similar to PIR|S51232|S51232 gibberellin-responsive ovar... 28 1.9
AJ497357 28 1.9
TC77479 similar to GP|19423874|gb|AAL87314.1 unknown protein {Ar... 28 1.9
TC76601 aquaporin-like protein PIP2 [Medicago truncatula] 28 3.3
TC76425 similar to SP|O65781|GAE2_CYATE UDP-glucose 4-epimerase ... 28 3.3
AJ502412 similar to GP|21592647|gb| unknown {Arabidopsis thalian... 28 3.3
BF650547 similar to GP|9758388|dbj| AP2 domain transcription fac... 28 3.3
AW774202 similar to PIR|T00517|T00 probable metal ion transporte... 27 4.3
TC86058 similar to GP|15912261|gb|AAL08264.1 AT4g27720/T29A15_21... 27 4.3
BQ151364 similar to GP|11993889|gb| virion-associated nuclear-sh... 27 5.6
TC80882 similar to PIR|G86319|G86319 hypothetical protein AAF984... 27 5.6
TC86474 weakly similar to GP|6016680|gb|AAF01507.1| putative leu... 27 5.6
>TC89284 similar to GP|1890154|emb|CAA72432.1 alpha-mannosidase precursor
{Arabidopsis thaliana}, partial (36%)
Length = 1246
Score = 40.4 bits (93), Expect = 5e-04
Identities = 17/48 (35%), Positives = 31/48 (64%), Gaps = 8/48 (16%)
Frame = +3
Query: 137 EKLKVFVVPHSHNDPGWKLTVEEYYDRQS--------RHILDTIVETL 176
+K+ V +VPHSH+D GW TV++YY + +++LD+++ +L
Sbjct: 141 DKINVHLVPHSHDDVGWLKTVDQYYVGSNNSIRGACVQNVLDSVISSL 284
>TC82621 similar to GP|10177664|dbj|BAB11126. alpha-mannosidase {Arabidopsis
thaliana}, partial (16%)
Length = 690
Score = 38.9 bits (89), Expect = 0.001
Identities = 16/39 (41%), Positives = 24/39 (61%)
Frame = +3
Query: 138 KLKVFVVPHSHNDPGWKLTVEEYYDRQSRHILDTIVETL 176
KL V +VPHSH+D GW T+++YY + I V+ +
Sbjct: 210 KLNVHLVPHSHDDVGWLKTIDQYYVGSNNTIQGACVQNV 326
>BI309315 similar to GP|10177664|db alpha-mannosidase {Arabidopsis thaliana},
partial (6%)
Length = 447
Score = 35.4 bits (80), Expect = 0.016
Identities = 15/39 (38%), Positives = 23/39 (58%)
Frame = +1
Query: 138 KLKVFVVPHSHNDPGWKLTVEEYYDRQSRHILDTIVETL 176
KL V +V H+H+D GW TV++YY + I V+ +
Sbjct: 265 KLNVHLVAHTHDDVGWLKTVDQYYVGSNNSIQGACVQNV 381
>TC84130 similar to GP|14326479|gb|AAK60285.1 AT5g56980/MHM17_10
{Arabidopsis thaliana}, partial (13%)
Length = 846
Score = 30.0 bits (66), Expect = 0.66
Identities = 22/67 (32%), Positives = 31/67 (45%)
Frame = +1
Query: 37 DFIFSNFFIIGLIISLLFFLIVLLRFGVPKPITTHFRTRTSRFRKPKKLSLNGSSTIFGG 96
D +F I+ + + FLIV L G IT+HF T+ R + SS+IFG
Sbjct: 19 DSVFIYDIIVSWVSASFIFLIVNLVIGTIA-ITSHFATQKKRQPNSPLELVRSSSSIFGR 195
Query: 97 FASVDLT 103
S L+
Sbjct: 196 VTSFGLS 216
>TC80530 weakly similar to PIR|S44261|S44261 SRG1 protein - Arabidopsis
thaliana, partial (52%)
Length = 1219
Score = 29.6 bits (65), Expect = 0.86
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +1
Query: 4 SSRRGGNWAQSILPSSNPKSKQPRKSKRRT 33
+ R+ NW +ILP SN + K+ ++RRT
Sbjct: 253 TGRKCENWCSTILPPSNGREKEVLANRRRT 342
>TC83679 weakly similar to PIR|G75403|G75403 DNA topoisomerase I -
Deinococcus radiodurans (strain R1), partial (3%)
Length = 596
Score = 29.6 bits (65), Expect = 0.86
Identities = 11/23 (47%), Positives = 16/23 (68%)
Frame = -3
Query: 181 FCLFPFLGFGDFYVYVCCYCCLF 203
+C+F F GFG + + CC+CC F
Sbjct: 297 WCIFGF-GFGWSFSWRCCWCCCF 232
>TC77254 similar to GP|10334497|emb|CAC10210. hypothetical protein {Cicer
arietinum}, partial (62%)
Length = 1985
Score = 29.3 bits (64), Expect = 1.1
Identities = 23/69 (33%), Positives = 39/69 (56%), Gaps = 5/69 (7%)
Frame = +2
Query: 34 LVKDFIFSNFFIIGLIISLLFFLIVLLRFGVPKPIT-----THFRTRTSRFRKPKKLSLN 88
L+ F+ + F IIG++ SLLFFL F PK I+ +++ T++S R + L+
Sbjct: 86 LLHRFLQNKFLIIGIVFSLLFFL-TSHSFYPPKFISDFSSISYYATKSSYSRINPHILLH 262
Query: 89 GSSTIFGGF 97
S+++F F
Sbjct: 263 -STSLFSDF 286
>TC84571 weakly similar to SP|P29136|MEP1_SOYBN Metalloendoproteinase 1
precursor (EC 3.4.24.-) (SMEP1). [Soybean] {Glycine
max}, partial (28%)
Length = 506
Score = 29.3 bits (64), Expect = 1.1
Identities = 18/56 (32%), Positives = 25/56 (44%), Gaps = 7/56 (12%)
Frame = +1
Query: 155 LTVEEYYDRQSR-HILDTIVETLSKVKFCLFPFLGFG------DFYVYVCCYCCLF 203
+T EEY R + H VET V FC+ FL G D Y +C + ++
Sbjct: 286 VTGEEYRQRWLQPHFFVVPVETFLSVDFCIITFLAIGIVFCLKDSYYRICLFTFMY 453
>TC87274 similar to PIR|S51232|S51232 gibberellin-responsive ovarian protein
G14 - garden pea (fragment), partial (45%)
Length = 734
Score = 28.5 bits (62), Expect = 1.9
Identities = 11/20 (55%), Positives = 14/20 (70%), Gaps = 1/20 (5%)
Frame = -3
Query: 186 FLGF-GDFYVYVCCYCCLFY 204
FL F G ++ + CCYCC FY
Sbjct: 501 FLCFCGGYFFHWCCYCC*FY 442
>AJ497357
Length = 666
Score = 28.5 bits (62), Expect = 1.9
Identities = 16/35 (45%), Positives = 21/35 (59%), Gaps = 9/35 (25%)
Frame = -3
Query: 48 LIISLLFFLIVLLRFGVPKP---------ITTHFR 73
+IIS FF+I+ L+FGVP P ITT F+
Sbjct: 466 IIISTTFFMIL*LKFGVPLPSQGCSLTITITTFFK 362
>TC77479 similar to GP|19423874|gb|AAL87314.1 unknown protein {Arabidopsis
thaliana}, partial (21%)
Length = 2241
Score = 28.5 bits (62), Expect = 1.9
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +3
Query: 33 TLVKDFIFSNFFIIGLIISLLFFLIVLL 60
TLVK I S +FI+ ++ +FF++V L
Sbjct: 2052 TLVKKIIRSKYFILPCVLGRIFFMLVYL 2135
>TC76601 aquaporin-like protein PIP2 [Medicago truncatula]
Length = 1497
Score = 27.7 bits (60), Expect = 3.3
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -2
Query: 184 FPFLGFGDFYVYVCCYCCLFYE 205
F FLG G + VY+C CC+ E
Sbjct: 308 FLFLGLGFWIVYLCFVCCVSEE 243
>TC76425 similar to SP|O65781|GAE2_CYATE UDP-glucose 4-epimerase GEPI48 (EC
5.1.3.2) (Galactowaldenase) (UDP- galactose
4-epimerase)., partial (58%)
Length = 738
Score = 27.7 bits (60), Expect = 3.3
Identities = 13/39 (33%), Positives = 20/39 (50%)
Frame = +1
Query: 149 NDPGWKLTVEEYYDRQSRHILDTIVETLSKVKFCLFPFL 187
+DPGWK+T+ Y++ H I E + L PF+
Sbjct: 241 SDPGWKITLLRYFNPVGAHPSGHIGEDPRGIPNNLMPFV 357
>AJ502412 similar to GP|21592647|gb| unknown {Arabidopsis thaliana}, partial
(20%)
Length = 436
Score = 27.7 bits (60), Expect = 3.3
Identities = 13/27 (48%), Positives = 16/27 (59%), Gaps = 2/27 (7%)
Frame = -3
Query: 179 VKFCLFP--FLGFGDFYVYVCCYCCLF 203
V FC F+ FGD +Y+CC C LF
Sbjct: 83 VFFCYLTVLFVCFGDR*IYMCCVCGLF 3
>BF650547 similar to GP|9758388|dbj| AP2 domain transcription factor-like
{Arabidopsis thaliana}, partial (24%)
Length = 418
Score = 27.7 bits (60), Expect = 3.3
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = -3
Query: 38 FIFSNFFIIGLIISLLFFLIVLLRFGVPKP 67
FIF FF F +V++R GVPKP
Sbjct: 158 FIFFLFFFAACSTEASFSFVVVIRRGVPKP 69
>AW774202 similar to PIR|T00517|T00 probable metal ion transporter (NRAMP)
[imported] - Arabidopsis thaliana, partial (20%)
Length = 564
Score = 27.3 bits (59), Expect = 4.3
Identities = 13/43 (30%), Positives = 24/43 (55%), Gaps = 4/43 (9%)
Frame = -1
Query: 24 KQPRKSKRRTLVK----DFIFSNFFIIGLIISLLFFLIVLLRF 62
K+ +K+ +R LV F+ F+ GL++SLL +++ F
Sbjct: 345 KKKKKTYKRLLVVLLLLGFLVMELFLFGLVLSLLLLIVLFFSF 217
>TC86058 similar to GP|15912261|gb|AAL08264.1 AT4g27720/T29A15_210
{Arabidopsis thaliana}, partial (96%)
Length = 1979
Score = 27.3 bits (59), Expect = 4.3
Identities = 14/35 (40%), Positives = 23/35 (65%)
Frame = -2
Query: 4 SSRRGGNWAQSILPSSNPKSKQPRKSKRRTLVKDF 38
SS GG+W++S + + SKQPRK+ +T+ K +
Sbjct: 772 SSIEGGHWSKSQCINKDI-SKQPRKNGNKTISKKY 671
>BQ151364 similar to GP|11993889|gb| virion-associated nuclear-shuttling
protein {Mus musculus}, partial (4%)
Length = 1113
Score = 26.9 bits (58), Expect = 5.6
Identities = 13/29 (44%), Positives = 16/29 (54%)
Frame = -3
Query: 173 VETLSKVKFCLFPFLGFGDFYVYVCCYCC 201
V +LS C+F GF YV+V C CC
Sbjct: 967 VRSLSVCVLCVFDAWGFR-VYVFVSCRCC 884
>TC80882 similar to PIR|G86319|G86319 hypothetical protein AAF98413.1
[imported] - Arabidopsis thaliana, partial (44%)
Length = 1075
Score = 26.9 bits (58), Expect = 5.6
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = +2
Query: 93 IFGGFASVDLTTKGLYDKIEFLDVDGGAWK 122
+FGGF + LY+ + LDVD G W+
Sbjct: 929 VFGGFTDA----QNLYNDLYTLDVDTGIWR 1006
>TC86474 weakly similar to GP|6016680|gb|AAF01507.1| putative
leucoanthocyanidin dioxygenase {Arabidopsis thaliana},
partial (20%)
Length = 1395
Score = 26.9 bits (58), Expect = 5.6
Identities = 18/59 (30%), Positives = 28/59 (46%)
Frame = -2
Query: 14 SILPSSNPKSKQPRKSKRRTLVKDFIFSNFFIIGLIISLLFFLIVLLRFGVPKPITTHF 72
SI+ S P S R +K T K+ + F+I L +LL+F ++ P+ T F
Sbjct: 1034 SII*SKGPSSASSRGNKNDT--KEVLLLFLFVIALCSTLLYFPLLSTSITSPRLTTKAF 864
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.326 0.143 0.458
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,299,006
Number of Sequences: 36976
Number of extensions: 131880
Number of successful extensions: 1768
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 1726
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1756
length of query: 208
length of database: 9,014,727
effective HSP length: 92
effective length of query: 116
effective length of database: 5,612,935
effective search space: 651100460
effective search space used: 651100460
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC141109.13