Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC141109.13 - phase: 0 
         (208 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC89284 similar to GP|1890154|emb|CAA72432.1 alpha-mannosidase p...    40  5e-04
TC82621 similar to GP|10177664|dbj|BAB11126. alpha-mannosidase {...    39  0.001
BI309315 similar to GP|10177664|db alpha-mannosidase {Arabidopsi...    35  0.016
TC84130 similar to GP|14326479|gb|AAK60285.1 AT5g56980/MHM17_10 ...    30  0.66
TC80530 weakly similar to PIR|S44261|S44261 SRG1 protein - Arabi...    30  0.86
TC83679 weakly similar to PIR|G75403|G75403 DNA topoisomerase I ...    30  0.86
TC77254 similar to GP|10334497|emb|CAC10210. hypothetical protei...    29  1.1
TC84571 weakly similar to SP|P29136|MEP1_SOYBN Metalloendoprotei...    29  1.1
TC87274 similar to PIR|S51232|S51232 gibberellin-responsive ovar...    28  1.9
AJ497357                                                               28  1.9
TC77479 similar to GP|19423874|gb|AAL87314.1 unknown protein {Ar...    28  1.9
TC76601 aquaporin-like protein PIP2 [Medicago truncatula]              28  3.3
TC76425 similar to SP|O65781|GAE2_CYATE UDP-glucose 4-epimerase ...    28  3.3
AJ502412 similar to GP|21592647|gb| unknown {Arabidopsis thalian...    28  3.3
BF650547 similar to GP|9758388|dbj| AP2 domain transcription fac...    28  3.3
AW774202 similar to PIR|T00517|T00 probable metal ion transporte...    27  4.3
TC86058 similar to GP|15912261|gb|AAL08264.1 AT4g27720/T29A15_21...    27  4.3
BQ151364 similar to GP|11993889|gb| virion-associated nuclear-sh...    27  5.6
TC80882 similar to PIR|G86319|G86319 hypothetical protein AAF984...    27  5.6
TC86474 weakly similar to GP|6016680|gb|AAF01507.1| putative leu...    27  5.6

>TC89284 similar to GP|1890154|emb|CAA72432.1 alpha-mannosidase precursor
           {Arabidopsis thaliana}, partial (36%)
          Length = 1246

 Score = 40.4 bits (93), Expect = 5e-04
 Identities = 17/48 (35%), Positives = 31/48 (64%), Gaps = 8/48 (16%)
 Frame = +3

Query: 137 EKLKVFVVPHSHNDPGWKLTVEEYYDRQS--------RHILDTIVETL 176
           +K+ V +VPHSH+D GW  TV++YY   +        +++LD+++ +L
Sbjct: 141 DKINVHLVPHSHDDVGWLKTVDQYYVGSNNSIRGACVQNVLDSVISSL 284


>TC82621 similar to GP|10177664|dbj|BAB11126. alpha-mannosidase {Arabidopsis
           thaliana}, partial (16%)
          Length = 690

 Score = 38.9 bits (89), Expect = 0.001
 Identities = 16/39 (41%), Positives = 24/39 (61%)
 Frame = +3

Query: 138 KLKVFVVPHSHNDPGWKLTVEEYYDRQSRHILDTIVETL 176
           KL V +VPHSH+D GW  T+++YY   +  I    V+ +
Sbjct: 210 KLNVHLVPHSHDDVGWLKTIDQYYVGSNNTIQGACVQNV 326


>BI309315 similar to GP|10177664|db alpha-mannosidase {Arabidopsis thaliana},
           partial (6%)
          Length = 447

 Score = 35.4 bits (80), Expect = 0.016
 Identities = 15/39 (38%), Positives = 23/39 (58%)
 Frame = +1

Query: 138 KLKVFVVPHSHNDPGWKLTVEEYYDRQSRHILDTIVETL 176
           KL V +V H+H+D GW  TV++YY   +  I    V+ +
Sbjct: 265 KLNVHLVAHTHDDVGWLKTVDQYYVGSNNSIQGACVQNV 381


>TC84130 similar to GP|14326479|gb|AAK60285.1 AT5g56980/MHM17_10
           {Arabidopsis thaliana}, partial (13%)
          Length = 846

 Score = 30.0 bits (66), Expect = 0.66
 Identities = 22/67 (32%), Positives = 31/67 (45%)
 Frame = +1

Query: 37  DFIFSNFFIIGLIISLLFFLIVLLRFGVPKPITTHFRTRTSRFRKPKKLSLNGSSTIFGG 96
           D +F    I+  + +   FLIV L  G    IT+HF T+  R        +  SS+IFG 
Sbjct: 19  DSVFIYDIIVSWVSASFIFLIVNLVIGTIA-ITSHFATQKKRQPNSPLELVRSSSSIFGR 195

Query: 97  FASVDLT 103
             S  L+
Sbjct: 196 VTSFGLS 216


>TC80530 weakly similar to PIR|S44261|S44261 SRG1 protein - Arabidopsis
           thaliana, partial (52%)
          Length = 1219

 Score = 29.6 bits (65), Expect = 0.86
 Identities = 12/30 (40%), Positives = 19/30 (63%)
 Frame = +1

Query: 4   SSRRGGNWAQSILPSSNPKSKQPRKSKRRT 33
           + R+  NW  +ILP SN + K+   ++RRT
Sbjct: 253 TGRKCENWCSTILPPSNGREKEVLANRRRT 342


>TC83679 weakly similar to PIR|G75403|G75403 DNA topoisomerase I -
           Deinococcus radiodurans (strain R1), partial (3%)
          Length = 596

 Score = 29.6 bits (65), Expect = 0.86
 Identities = 11/23 (47%), Positives = 16/23 (68%)
 Frame = -3

Query: 181 FCLFPFLGFGDFYVYVCCYCCLF 203
           +C+F F GFG  + + CC+CC F
Sbjct: 297 WCIFGF-GFGWSFSWRCCWCCCF 232


>TC77254 similar to GP|10334497|emb|CAC10210. hypothetical protein {Cicer
           arietinum}, partial (62%)
          Length = 1985

 Score = 29.3 bits (64), Expect = 1.1
 Identities = 23/69 (33%), Positives = 39/69 (56%), Gaps = 5/69 (7%)
 Frame = +2

Query: 34  LVKDFIFSNFFIIGLIISLLFFLIVLLRFGVPKPIT-----THFRTRTSRFRKPKKLSLN 88
           L+  F+ + F IIG++ SLLFFL     F  PK I+     +++ T++S  R    + L+
Sbjct: 86  LLHRFLQNKFLIIGIVFSLLFFL-TSHSFYPPKFISDFSSISYYATKSSYSRINPHILLH 262

Query: 89  GSSTIFGGF 97
            S+++F  F
Sbjct: 263 -STSLFSDF 286


>TC84571 weakly similar to SP|P29136|MEP1_SOYBN Metalloendoproteinase 1
           precursor (EC 3.4.24.-) (SMEP1). [Soybean] {Glycine
           max}, partial (28%)
          Length = 506

 Score = 29.3 bits (64), Expect = 1.1
 Identities = 18/56 (32%), Positives = 25/56 (44%), Gaps = 7/56 (12%)
 Frame = +1

Query: 155 LTVEEYYDRQSR-HILDTIVETLSKVKFCLFPFLGFG------DFYVYVCCYCCLF 203
           +T EEY  R  + H     VET   V FC+  FL  G      D Y  +C +  ++
Sbjct: 286 VTGEEYRQRWLQPHFFVVPVETFLSVDFCIITFLAIGIVFCLKDSYYRICLFTFMY 453


>TC87274 similar to PIR|S51232|S51232 gibberellin-responsive ovarian protein
           G14 - garden pea (fragment), partial (45%)
          Length = 734

 Score = 28.5 bits (62), Expect = 1.9
 Identities = 11/20 (55%), Positives = 14/20 (70%), Gaps = 1/20 (5%)
 Frame = -3

Query: 186 FLGF-GDFYVYVCCYCCLFY 204
           FL F G ++ + CCYCC FY
Sbjct: 501 FLCFCGGYFFHWCCYCC*FY 442


>AJ497357 
          Length = 666

 Score = 28.5 bits (62), Expect = 1.9
 Identities = 16/35 (45%), Positives = 21/35 (59%), Gaps = 9/35 (25%)
 Frame = -3

Query: 48  LIISLLFFLIVLLRFGVPKP---------ITTHFR 73
           +IIS  FF+I+ L+FGVP P         ITT F+
Sbjct: 466 IIISTTFFMIL*LKFGVPLPSQGCSLTITITTFFK 362


>TC77479 similar to GP|19423874|gb|AAL87314.1 unknown protein {Arabidopsis
            thaliana}, partial (21%)
          Length = 2241

 Score = 28.5 bits (62), Expect = 1.9
 Identities = 12/28 (42%), Positives = 19/28 (67%)
 Frame = +3

Query: 33   TLVKDFIFSNFFIIGLIISLLFFLIVLL 60
            TLVK  I S +FI+  ++  +FF++V L
Sbjct: 2052 TLVKKIIRSKYFILPCVLGRIFFMLVYL 2135


>TC76601 aquaporin-like protein PIP2 [Medicago truncatula]
          Length = 1497

 Score = 27.7 bits (60), Expect = 3.3
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = -2

Query: 184 FPFLGFGDFYVYVCCYCCLFYE 205
           F FLG G + VY+C  CC+  E
Sbjct: 308 FLFLGLGFWIVYLCFVCCVSEE 243


>TC76425 similar to SP|O65781|GAE2_CYATE UDP-glucose 4-epimerase GEPI48 (EC
           5.1.3.2) (Galactowaldenase) (UDP- galactose
           4-epimerase)., partial (58%)
          Length = 738

 Score = 27.7 bits (60), Expect = 3.3
 Identities = 13/39 (33%), Positives = 20/39 (50%)
 Frame = +1

Query: 149 NDPGWKLTVEEYYDRQSRHILDTIVETLSKVKFCLFPFL 187
           +DPGWK+T+  Y++    H    I E    +   L PF+
Sbjct: 241 SDPGWKITLLRYFNPVGAHPSGHIGEDPRGIPNNLMPFV 357


>AJ502412 similar to GP|21592647|gb| unknown {Arabidopsis thaliana}, partial
           (20%)
          Length = 436

 Score = 27.7 bits (60), Expect = 3.3
 Identities = 13/27 (48%), Positives = 16/27 (59%), Gaps = 2/27 (7%)
 Frame = -3

Query: 179 VKFCLFP--FLGFGDFYVYVCCYCCLF 203
           V FC     F+ FGD  +Y+CC C LF
Sbjct: 83  VFFCYLTVLFVCFGDR*IYMCCVCGLF 3


>BF650547 similar to GP|9758388|dbj| AP2 domain transcription factor-like
           {Arabidopsis thaliana}, partial (24%)
          Length = 418

 Score = 27.7 bits (60), Expect = 3.3
 Identities = 13/30 (43%), Positives = 16/30 (53%)
 Frame = -3

Query: 38  FIFSNFFIIGLIISLLFFLIVLLRFGVPKP 67
           FIF  FF         F  +V++R GVPKP
Sbjct: 158 FIFFLFFFAACSTEASFSFVVVIRRGVPKP 69


>AW774202 similar to PIR|T00517|T00 probable metal ion transporter (NRAMP)
           [imported] - Arabidopsis thaliana, partial (20%)
          Length = 564

 Score = 27.3 bits (59), Expect = 4.3
 Identities = 13/43 (30%), Positives = 24/43 (55%), Gaps = 4/43 (9%)
 Frame = -1

Query: 24  KQPRKSKRRTLVK----DFIFSNFFIIGLIISLLFFLIVLLRF 62
           K+ +K+ +R LV      F+    F+ GL++SLL  +++   F
Sbjct: 345 KKKKKTYKRLLVVLLLLGFLVMELFLFGLVLSLLLLIVLFFSF 217


>TC86058 similar to GP|15912261|gb|AAL08264.1 AT4g27720/T29A15_210
           {Arabidopsis thaliana}, partial (96%)
          Length = 1979

 Score = 27.3 bits (59), Expect = 4.3
 Identities = 14/35 (40%), Positives = 23/35 (65%)
 Frame = -2

Query: 4   SSRRGGNWAQSILPSSNPKSKQPRKSKRRTLVKDF 38
           SS  GG+W++S   + +  SKQPRK+  +T+ K +
Sbjct: 772 SSIEGGHWSKSQCINKDI-SKQPRKNGNKTISKKY 671


>BQ151364 similar to GP|11993889|gb| virion-associated nuclear-shuttling
           protein {Mus musculus}, partial (4%)
          Length = 1113

 Score = 26.9 bits (58), Expect = 5.6
 Identities = 13/29 (44%), Positives = 16/29 (54%)
 Frame = -3

Query: 173 VETLSKVKFCLFPFLGFGDFYVYVCCYCC 201
           V +LS    C+F   GF   YV+V C CC
Sbjct: 967 VRSLSVCVLCVFDAWGFR-VYVFVSCRCC 884


>TC80882 similar to PIR|G86319|G86319 hypothetical protein AAF98413.1
           [imported] - Arabidopsis thaliana, partial (44%)
          Length = 1075

 Score = 26.9 bits (58), Expect = 5.6
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = +2

Query: 93  IFGGFASVDLTTKGLYDKIEFLDVDGGAWK 122
           +FGGF       + LY+ +  LDVD G W+
Sbjct: 929 VFGGFTDA----QNLYNDLYTLDVDTGIWR 1006


>TC86474 weakly similar to GP|6016680|gb|AAF01507.1| putative
            leucoanthocyanidin dioxygenase {Arabidopsis thaliana},
            partial (20%)
          Length = 1395

 Score = 26.9 bits (58), Expect = 5.6
 Identities = 18/59 (30%), Positives = 28/59 (46%)
 Frame = -2

Query: 14   SILPSSNPKSKQPRKSKRRTLVKDFIFSNFFIIGLIISLLFFLIVLLRFGVPKPITTHF 72
            SI+ S  P S   R +K  T  K+ +    F+I L  +LL+F ++      P+  T  F
Sbjct: 1034 SII*SKGPSSASSRGNKNDT--KEVLLLFLFVIALCSTLLYFPLLSTSITSPRLTTKAF 864


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.326    0.143    0.458 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,299,006
Number of Sequences: 36976
Number of extensions: 131880
Number of successful extensions: 1768
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 1726
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1756
length of query: 208
length of database: 9,014,727
effective HSP length: 92
effective length of query: 116
effective length of database: 5,612,935
effective search space: 651100460
effective search space used: 651100460
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 56 (26.2 bits)


Medicago: description of AC141109.13