BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC141106.11 + phase: 0 /pseudo
(333 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77037 similar to PIR|B84652|B84652 hypothetical protein At2g25... 131 3e-31
TC77654 similar to GP|22137236|gb|AAM91463.1 At2g36630/F1O11.26 ... 117 6e-27
TC88574 weakly similar to GP|22137236|gb|AAM91463.1 At2g36630/F1... 114 4e-26
TC88695 similar to GP|13272465|gb|AAK17171.1 unknown protein {Ar... 56 2e-08
TC82901 similar to GP|22137236|gb|AAM91463.1 At2g36630/F1O11.26 ... 40 0.002
TC84534 weakly similar to PIR|B84652|B84652 hypothetical protein... 38 0.005
TC85544 homologue to GP|6984142|gb|AAF34771.1| 40S ribosomal pro... 28 4.9
TC88015 similar to GP|13926206|gb|AAK49581.1 Unknown protein {Ar... 28 4.9
CB893164 weakly similar to GP|6016718|gb| hypothetical protein {... 27 8.4
>TC77037 similar to PIR|B84652|B84652 hypothetical protein At2g25740
[imported] - Arabidopsis thaliana, partial (33%)
Length = 2114
Score = 131 bits (330), Expect = 3e-31
Identities = 60/90 (66%), Positives = 72/90 (79%)
Frame = +2
Query: 1 MITGGAGATVLYNLKKRHPTLDLPVIDYDLALLFQPMLMLGISLGVAFNLIFPDWMLTTL 60
MI G A +TV YNL+ RHPTLD+P+IDYDLALL QPMLMLGIS+GV FN++FPDW++T L
Sbjct: 764 MIMGAALSTVYYNLRLRHPTLDMPIIDYDLALLIQPMLMLGISIGVVFNVVFPDWLVTIL 943
Query: 61 LIIFFTGISVNAFLKGVNTWKKETLTKKVA 90
LI+ F G S AF KG+ TW KET+ KK A
Sbjct: 944 LIVLFLGTSTKAFFKGLETWNKETIMKKEA 1033
Score = 76.6 bits (187), Expect = 1e-14
Identities = 62/124 (50%), Positives = 65/124 (52%)
Frame = +3
Query: 202 *SVACLVLVEDLS*GHFSLDLESLLRWQVLHPLLQ*HFLHLCLWWNITF*NVFQFHMLFT 261
*SV C DL F ESLL+ Q PLLQ*H LCL WN T *N FQF MLFT
Sbjct: 1404 *SVDCWD*EADLLWVRFF*S*ESLLKCQAPQPLLQ*HSPRLCLLWNTTC*NDFQFLMLFT 1583
Query: 262 L*L*PLLLHLLGNIW*ESLLPY*EEHQLSFSS*PSQFL*VVYH*VEQA*QI*LKGLKTKS 321
LL L NI *E Y EEH S *P+Q+L V H*VE A QI L S
Sbjct: 1584 *AWWLLLQPWLDNIL*EG*SSYLEEHHSSSLF*PAQYLSVQSH*VELAFQIWCTRLPIMS 1763
Query: 322 TWDL 325
WDL
Sbjct: 1764 IWDL 1775
>TC77654 similar to GP|22137236|gb|AAM91463.1 At2g36630/F1O11.26
{Arabidopsis thaliana}, partial (94%)
Length = 1939
Score = 117 bits (293), Expect = 6e-27
Identities = 52/88 (59%), Positives = 69/88 (78%)
Frame = +2
Query: 1 MITGGAGATVLYNLKKRHPTLDLPVIDYDLALLFQPMLMLGISLGVAFNLIFPDWMLTTL 60
MI G + ++V YNL+ RHPT D+P++DYDLALLFQPMLMLGI++GVA +++FP W++T L
Sbjct: 389 MIMGASTSSVFYNLRVRHPTKDVPILDYDLALLFQPMLMLGITVGVALSVVFPYWLITVL 568
Query: 61 LIIFFTGISVNAFLKGVNTWKKETLTKK 88
+II F G S +F KG WK+ETL KK
Sbjct: 569 IIILFIGTSSRSFFKGTEMWKEETLLKK 652
Score = 32.7 bits (73), Expect = 0.20
Identities = 44/127 (34%), Positives = 54/127 (41%), Gaps = 4/127 (3%)
Frame = +3
Query: 207 LVLVEDLS*GHFSLDLESLLRWQVLHPLLQ*HFLHLCLWWNITF*NVFQFHMLFTL*L*P 266
LVL D FSL S L+ V L * F H CLW N T+ F M T
Sbjct: 1071 LVLEVDSFWALFSLRSVSSLKLLVQQRHL**CFHHHCLWLNSTYSRGSPFPMHCTSSQCL 1250
Query: 267 LLLHLLGNIW*ESLLPY*EEHQLSFSS*PSQFL*VVYH*VEQA*QI*LKG----LKTKST 322
L L + *E+ L + EE Q +SS FL V+ V A LKG +T +
Sbjct: 1251 FWLALWDSYL*ENCLEFLEEPQSLYSSSQGSFLLVLSPWV*LA----LKGAFI*YRTMNL 1418
Query: 323 WDLVTYV 329
WD +V
Sbjct: 1419 WDF*AFV 1439
>TC88574 weakly similar to GP|22137236|gb|AAM91463.1 At2g36630/F1O11.26
{Arabidopsis thaliana}, partial (25%)
Length = 663
Score = 114 bits (286), Expect = 4e-26
Identities = 52/87 (59%), Positives = 69/87 (78%)
Frame = +3
Query: 1 MITGGAGATVLYNLKKRHPTLDLPVIDYDLALLFQPMLMLGISLGVAFNLIFPDWMLTTL 60
M+TG A +TV +NLK RHPTLD+P+IDYDL LL P+++LGIS+GV +++F DWM+T L
Sbjct: 402 MVTGAAISTVFFNLKLRHPTLDIPMIDYDLVLLNAPVIILGISIGVVLSVVFADWMITVL 581
Query: 61 LIIFFTGISVNAFLKGVNTWKKETLTK 87
LII F SV A+LKG++TW KET+ K
Sbjct: 582 LIIVFIVTSVRAYLKGLDTWNKETIIK 662
>TC88695 similar to GP|13272465|gb|AAK17171.1 unknown protein {Arabidopsis
thaliana}, partial (24%)
Length = 591
Score = 55.8 bits (133), Expect = 2e-08
Identities = 26/65 (40%), Positives = 42/65 (64%), Gaps = 1/65 (1%)
Frame = +2
Query: 1 MITGGAGATVL-YNLKKRHPTLDLPVIDYDLALLFQPMLMLGISLGVAFNLIFPDWMLTT 59
M+TGG+ A V+ Y +IDYD+AL +P ++LG+S+GV NL+FP+W++T
Sbjct: 380 MVTGGSIANVICYMFTTSTKFGGKSLIDYDIALSSEPCMLLGVSVGVICNLVFPEWLITL 559
Query: 60 LLIIF 64
+ +F
Sbjct: 560 MFAVF 574
>TC82901 similar to GP|22137236|gb|AAM91463.1 At2g36630/F1O11.26
{Arabidopsis thaliana}, partial (22%)
Length = 647
Score = 39.7 bits (91), Expect = 0.002
Identities = 19/52 (36%), Positives = 31/52 (59%)
Frame = +3
Query: 1 MITGGAGATVLYNLKKRHPTLDLPVIDYDLALLFQPMLMLGISLGVAFNLIF 52
MI G + ++V YNL+ RHPT D+P++D + F + +SL V ++F
Sbjct: 483 MIMGASTSSVFYNLRVRHPTKDVPILDL*SSSSFSTYALCLVSLLVLLLMLF 638
Score = 27.7 bits (60), Expect = 6.4
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +2
Query: 34 FQPMLMLGISLGVAFNLIFP 53
F MLGI++GVA N++FP
Sbjct: 584 FNLCFMLGITVGVALNVVFP 643
>TC84534 weakly similar to PIR|B84652|B84652 hypothetical protein At2g25740
[imported] - Arabidopsis thaliana, partial (9%)
Length = 505
Score = 38.1 bits (87), Expect = 0.005
Identities = 42/104 (40%), Positives = 52/104 (49%), Gaps = 4/104 (3%)
Frame = +2
Query: 229 QVLHPLLQ*HFLHLCLWWNITF*NVFQFHMLFTL*L*PLLLHLLGNIW*ESL----LPY* 284
QV P +*H HL L +IT *NVF F ML T P L L +SL + +
Sbjct: 11 QVPQPH*E*HSQHLYLLHSITS*NVFLFLMLSTS---P*CLPLQHT*DRKSLTSLSISFK 181
Query: 285 EEHQLSFSS*PSQFL*VVYH*VEQA*QI*LKGLKTKSTWDLVTY 328
E LS PSQFL V H*VE * +G + +TWDL ++
Sbjct: 182 ELP*LSLFY-PSQFLLVQLH*VE*EYHA*FRGFRIMNTWDLRSF 310
>TC85544 homologue to GP|6984142|gb|AAF34771.1| 40S ribosomal protein S11
{Euphorbia esula}, partial (76%)
Length = 1335
Score = 28.1 bits (61), Expect = 4.9
Identities = 15/46 (32%), Positives = 22/46 (47%)
Frame = -3
Query: 28 YDLALLFQPMLMLGISLGVAFNLIFPDWMLTTLLIIFFTGISVNAF 73
YDL LL +L I L F +F + L ++FF + V+ F
Sbjct: 592 YDLTLLLAERFLLHIKLASLFFFLFLQLLCVCLCLMFFLVLVVSLF 455
>TC88015 similar to GP|13926206|gb|AAK49581.1 Unknown protein {Arabidopsis
thaliana}, partial (57%)
Length = 741
Score = 28.1 bits (61), Expect = 4.9
Identities = 11/35 (31%), Positives = 19/35 (53%)
Frame = +1
Query: 46 VAFNLIFPDWMLTTLLIIFFTGISVNAFLKGVNTW 80
V F F W+L T++++F +S+ L +N W
Sbjct: 586 VVFLCPFSQWLLHTIVLLFILILSIKLLLSFLNFW 690
>CB893164 weakly similar to GP|6016718|gb| hypothetical protein {Arabidopsis
thaliana}, partial (45%)
Length = 818
Score = 27.3 bits (59), Expect = 8.4
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = -3
Query: 219 SLDLESLLRWQVLHPLLQ*HFLH 241
SLDL ++L QVL PL+ HF H
Sbjct: 771 SLDLFAMLYCQVLQPLVSLHFSH 703
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.369 0.171 0.620
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,451,576
Number of Sequences: 36976
Number of extensions: 165318
Number of successful extensions: 2126
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1188
Number of HSP's successfully gapped in prelim test: 78
Number of HSP's that attempted gapping in prelim test: 922
Number of HSP's gapped (non-prelim): 1298
length of query: 333
length of database: 9,014,727
effective HSP length: 97
effective length of query: 236
effective length of database: 5,428,055
effective search space: 1281020980
effective search space used: 1281020980
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 36 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC141106.11