BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140027.12 + phase: 0 /pseudo
(526 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC82688 weakly similar to GP|13477193|gb|AAH05058.1 Similar to C... 317 6e-96
TC88439 weakly similar to PIR|E84546|E84546 probable tetracyclin... 191 6e-49
CA922730 similar to PIR|F84546|F845 probable tetracycline transp... 185 4e-47
BI272694 weakly similar to PIR|G84546|G845 probable tetracycline... 83 3e-16
BE187632 weakly similar to PIR|F86258|F862 protein F5O11.14 [imp... 45 9e-05
BQ752029 GP|12002184|g tetracycline resistance protein {IncN pla... 42 4e-04
BE318703 weakly similar to GP|10177006|dbj contains similarity t... 35 7e-04
TC76669 GP|4309724|gb|AAD15494.1| unknown protein {Arabidopsis t... 30 2.3
AL376009 similar to GP|22535713|db hypothetical protein~similar ... 30 2.3
BF638007 similar to GP|22136698|gb| putative cyclic nucleotide a... 28 6.6
TC91900 similar to GP|10728183|gb|AAF48000.2 CG11122 gene produc... 28 8.6
TC87126 similar to GP|15724240|gb|AAL06513.1 At1g75220/F22H5_6 {... 28 8.6
BQ157994 28 8.6
AW691772 28 8.6
TC85673 similar to SP|O81361|RS8_PRUAR 40S ribosomal protein S8.... 28 8.6
>TC82688 weakly similar to GP|13477193|gb|AAH05058.1 Similar to CG5078 gene
product {Homo sapiens}, partial (30%)
Length = 734
Score = 317 bits (811), Expect(2) = 6e-96
Identities = 153/178 (85%), Positives = 167/178 (92%)
Frame = +3
Query: 1 MSWWSGFFELKPLFHLLLPLSIHWIAEAMTVSVLVDVTTTALCPQQSSCSKAIYINGLQQ 60
MSWWSGFFEL+PLFHLLLPLSIHWIAE MTVSVLVDVTTTALCPQQSSCSKAIYINGLQ+
Sbjct: 75 MSWWSGFFELRPLFHLLLPLSIHWIAEEMTVSVLVDVTTTALCPQQSSCSKAIYINGLQE 254
Query: 61 TITGIFKMAVLPLLGQLSDEHGRKPLLLLTISTSIIPFALLAWNQSKEFVYAYYVLRTFS 120
TI GIFKM VLPLLGQLSD+HGRKP LLLT+ST+I PFALLAWNQS+EFVYAYYVLRT S
Sbjct: 255 TIAGIFKMMVLPLLGQLSDDHGRKPFLLLTMSTTIFPFALLAWNQSEEFVYAYYVLRTIS 434
Query: 121 HIISQGSIFCISVAYVADVVHESKRVAVFSWITGLSSAAHVIANVFARFLPQNYIFVI 178
+IIS+GSIFCISVAYVA+VV+E+KR AVF WITGL SA+HV+ NV ARFLPQNYIFV+
Sbjct: 435 YIISKGSIFCISVAYVANVVNENKRAAVFGWITGLFSASHVVGNVLARFLPQNYIFVV 608
Score = 53.1 bits (126), Expect(2) = 6e-96
Identities = 25/34 (73%), Positives = 30/34 (87%)
Frame = +2
Query: 187 GFNNTIDLLSTLYAFLPS*NCETGSRKEPRVRLL 220
GFN+TID+LS+LYA PS*N +T S+KEPRVRLL
Sbjct: 605 GFNSTIDILSSLYAIFPS*NWKTSSQKEPRVRLL 706
>TC88439 weakly similar to PIR|E84546|E84546 probable tetracycline
transporter protein [imported] - Arabidopsis thaliana,
partial (37%)
Length = 1399
Score = 191 bits (485), Expect = 6e-49
Identities = 144/508 (28%), Positives = 226/508 (44%)
Frame = +2
Query: 6 GFFELKPLFHLLLPLSIHWIAEAMTVSVLVDVTTTALCPQQSSCSKAIYINGLQQTITGI 65
G L L HL + + + + + + DVT ALCP Q CS AIY++G QQ + GI
Sbjct: 32 GMEGLTGLSHLFVTMFLAGFGGIIVMPSITDVTMAALCPGQDECSLAIYLSGFQQVMIGI 211
Query: 66 FKMAVLPLLGQLSDEHGRKPLLLLTISTSIIPFALLAWNQSKEFVYAYYVLRTFSHIISQ 125
+ PL+G LSD +GRK L+ L ++ S+IP A+LA+++ +F YAYYV++T + + +
Sbjct: 212 GSVMTTPLIGNLSDRYGRKALITLPLTVSLIPQAILAYSRDTKFFYAYYVVKTLAALAGE 391
Query: 126 GSIFCISVAYVADVVHESKRVAVFSWITGLSSAAHVIANVFARFLPQNYIFVILNYGLLL 185
GS C+++AYVAD+V KR + F + G+ SA+ V + ARFL F + + +
Sbjct: 392 GSFHCLALAYVADIVPVGKRASAFGILAGVGSASFVGGTLAARFLSAALTFQVAS----V 559
Query: 186 SGFNNTIDLLSTLYAFLPS*NCETGSRKEPRVRLLY*I*GMPQK**SSGCLSHVN*HQSF 245
S + + L P +EP + +PQ+ S
Sbjct: 560 SSMIGLVYMRIFLKESAPMRQPLLKEAEEPCIEQCE--DDLPQR--------TFKKLPSM 709
Query: 246 FELTCIHES*IFPTLRGVALVSFFYKLGMTGIHSVLLQIFSLLCHYYLTNFF**IP*YNR 305
+L C+ + PT A+V F L G +V + YYL F +
Sbjct: 710 GDLICLLK--CSPTFSQAAIVLLFNSLADGGSMAVTM--------YYLKARF-------Q 838
Query: 306 QSHNSYAVLFESCFWFQQKSVLRTSDDGRNWFHLFSARFQIVLLPILNPLVGEKVILCSA 365
+ N +A ++ S G LF L+PIL P VGE+ +L +
Sbjct: 839 YNKNQFA------------DLMMISGIGATLTQLF-------LMPILVPAVGEEKLLTTG 961
Query: 366 LLASIAYAWLSGLAWAPWVSKIVTFYINLIQIYMFIWLAENFITYISIMKVIEFNPLWQV 425
L S + ++W+ W V
Sbjct: 962 LFVSCISMLMYSISWSAW-----------------------------------------V 1018
Query: 426 PYLGGSFGIIYILEKPATYGIISKASSSTNQGKAQTFIAGANSISGLLSPIVMSPLTSLF 485
PY F ++ +L +P+ I SK QG Q ++G S + ++SP++ SPLT+LF
Sbjct: 1019PYALAGFSVLGVLVRPSLTSIASKQVGPNEQGMVQGCLSGITSAANIISPLIFSPLTALF 1198
Query: 486 LSSDAPFECKGFSIICASVCMVSYLFLS 513
LS DAPF GFS++C + +++ FLS
Sbjct: 1199LSEDAPFNFPGFSLMCLGLTLMTAFFLS 1282
>CA922730 similar to PIR|F84546|F845 probable tetracycline transporter
protein [imported] - Arabidopsis thaliana, partial (6%)
Length = 757
Score = 185 bits (469), Expect = 4e-47
Identities = 103/157 (65%), Positives = 108/157 (68%), Gaps = 1/157 (0%)
Frame = -2
Query: 356 VGEKVILCSALLASIAYAWLSGLAWAPWVSKIVTFYINLIQIYMFIWLAENFITYISIMK 415
VGEKVILCSALLASIAYAWLSGLAWAPWV
Sbjct: 756 VGEKVILCSALLASIAYAWLSGLAWAPWV------------------------------- 670
Query: 416 VIEFNPLWQVPYLGGSFGIIYILEKPATYGIISKASSSTNQGKAQTFIAGANSISGLLSP 475
PYL SFGIIY+L KPATY IIS+ASSSTNQGKAQTFIAGA SIS LLSP
Sbjct: 669 ----------PYLSASFGIIYVLVKPATYAIISRASSSTNQGKAQTFIAGAQSISDLLSP 520
Query: 476 IVMSPLTSLFLSSDAPFECKGFSIICASVC-MVSYLF 511
IVMSPLTSLFLSSDAPFECKGFSI+CASVC M+S +F
Sbjct: 519 IVMSPLTSLFLSSDAPFECKGFSILCASVCMMISLIF 409
>BI272694 weakly similar to PIR|G84546|G845 probable tetracycline transporter
protein [imported] - Arabidopsis thaliana, partial (17%)
Length = 648
Score = 82.8 bits (203), Expect = 3e-16
Identities = 50/167 (29%), Positives = 75/167 (43%), Gaps = 1/167 (0%)
Frame = +1
Query: 345 QIVLLPILNPLVGEKVILCSALLASIAYAWLSGLAWAPWVSKIVTFYINLIQIYMFIWLA 404
Q+ L+PI P +GE +L L + ++ +AW+ WV
Sbjct: 154 QLFLMPIFAPTLGEARLLSIGLFFHCVHMFIYSIAWSSWV-------------------- 273
Query: 405 ENFITYISIMKVIEFNPLWQVPYLGGSFGIIYILEKPATYGIISKASSSTNQGKAQTFIA 464
PY F I+++ +P I+SK QG+AQ I+
Sbjct: 274 ---------------------PYAAAMFSILFVFSQPCIRSIVSKQVDPREQGRAQGCIS 390
Query: 465 GANSISGLLSPIVMSPLTSLFLSSDAPFECKGFSIICASVC-MVSYL 510
G SI+ ++SP+ SPLT+LFLS APF GFSI+C + MVS++
Sbjct: 391 GICSIAHIVSPLAFSPLTALFLSEKAPFNFPGFSIMCIGIASMVSFV 531
>BE187632 weakly similar to PIR|F86258|F862 protein F5O11.14 [imported] -
Arabidopsis thaliana, partial (7%)
Length = 606
Score = 44.7 bits (104), Expect = 9e-05
Identities = 23/25 (92%), Positives = 24/25 (96%)
Frame = +3
Query: 346 IVLLPILNPLVGEKVILCSALLASI 370
IV+LPILNPLVGEKVIL SALLASI
Sbjct: 279 IVMLPILNPLVGEKVILFSALLASI 353
>BQ752029 GP|12002184|g tetracycline resistance protein {IncN plasmid R46},
partial (47%)
Length = 645
Score = 42.4 bits (98), Expect = 4e-04
Identities = 29/109 (26%), Positives = 52/109 (47%)
Frame = +2
Query: 57 GLQQTITGIFKMAVLPLLGQLSDEHGRKPLLLLTISTSIIPFALLAWNQSKEFVYAYYVL 116
G+ + + + P+LG LSD GR+P+LL ++ + I +A++A +YA ++
Sbjct: 218 GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRIV 397
Query: 117 RTFSHIISQGSIFCISVAYVADVVHESKRVAVFSWITGLSSAAHVIANV 165
+ G+ ++ AY+AD+ R F GL SA + V
Sbjct: 398 AGIT-----GATGAVAGAYIADITDGEDRARHF----GLMSACFGVGMV 517
>BE318703 weakly similar to GP|10177006|dbj contains similarity to
tetracycline transporter protein~gene_id:K5J14.1
{Arabidopsis thaliana}, partial (17%)
Length = 588
Score = 35.4 bits (80), Expect(2) = 7e-04
Identities = 15/40 (37%), Positives = 24/40 (59%)
Frame = -2
Query: 345 QIVLLPILNPLVGEKVILCSALLASIAYAWLSGLAWAPWV 384
Q+ L+PIL P VGE+ +L + L S + ++W+ WV
Sbjct: 458 QLFLMPILVPAVGEEKLLTTGLFVSCISMLMYSISWSAWV 339
Score = 25.4 bits (54), Expect(2) = 7e-04
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = -1
Query: 313 VLFESCFWFQQKSVLRTSDDGRNW 336
VLFE + KSV R DD R+W
Sbjct: 543 VLFEGTVFNTTKSVCRFDDDFRDW 472
>TC76669 GP|4309724|gb|AAD15494.1| unknown protein {Arabidopsis thaliana},
partial (14%)
Length = 839
Score = 30.0 bits (66), Expect = 2.3
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +1
Query: 485 FLSSDAPFECKGFSIICASVCMVSYLFLSPIHHL 518
FL A C G S++C C+ +LFL P++ L
Sbjct: 622 FLREIARGFCPGSSLVCGCCCLALFLFLVPLYIL 723
>AL376009 similar to GP|22535713|db hypothetical protein~similar to
Arabidopsis thaliana chromosome 2 At2g40870, partial
(18%)
Length = 522
Score = 30.0 bits (66), Expect = 2.3
Identities = 14/43 (32%), Positives = 23/43 (52%)
Frame = -1
Query: 476 IVMSPLTSLFLSSDAPFECKGFSIICASVCMVSYLFLSPIHHL 518
++ S TSL ++ ++ CKGFS+I S + SPI +
Sbjct: 213 LITSHRTSLAMTRNSSSACKGFSVISGSAVTAGFRSSSPIERV 85
>BF638007 similar to GP|22136698|gb| putative cyclic nucleotide and
calmodulin-regulated ion channel protein {Arabidopsis
thaliana}, partial (26%)
Length = 636
Score = 28.5 bits (62), Expect = 6.6
Identities = 24/76 (31%), Positives = 31/76 (40%), Gaps = 6/76 (7%)
Frame = -3
Query: 443 TYGIISKASSSTNQGKAQT------FIAGANSISGLLSPIVMSPLTSLFLSSDAPFECKG 496
T+GI S AS N +A A S+ +LSP V S LTSL E +
Sbjct: 409 TFGIFSSASRRRNAAHLHLPAIRPPRMAAAWSLLLILSPAVASSLTSLLFFLYLRSEFQI 230
Query: 497 FSIICASVCMVSYLFL 512
F C ++ FL
Sbjct: 229 FHYFCLFYFLIKIFFL 182
>TC91900 similar to GP|10728183|gb|AAF48000.2 CG11122 gene product
{Drosophila melanogaster}, partial (0%)
Length = 701
Score = 28.1 bits (61), Expect = 8.6
Identities = 13/38 (34%), Positives = 24/38 (62%)
Frame = +2
Query: 166 FARFLPQNYIFVILNYGLLLSGFNNTIDLLSTLYAFLP 203
F F+ ++++FV+ ++ L S + +DLLS + FLP
Sbjct: 83 FNLFINKHFMFVVFSF*FLFSFW*MELDLLSLINLFLP 196
>TC87126 similar to GP|15724240|gb|AAL06513.1 At1g75220/F22H5_6 {Arabidopsis
thaliana}, partial (79%)
Length = 1524
Score = 28.1 bits (61), Expect = 8.6
Identities = 17/71 (23%), Positives = 36/71 (49%)
Frame = +3
Query: 75 GQLSDEHGRKPLLLLTISTSIIPFALLAWNQSKEFVYAYYVLRTFSHIISQGSIFCISVA 134
GQ+++ GRK L++ +II + +++ + F++ +L F G I +
Sbjct: 33 GQIAEYVGRKGSLMIASIPNIIGWLAISFAKDSSFLFMGRLLEGF----GVGIISYVVPV 200
Query: 135 YVADVVHESKR 145
Y+A++ E+ R
Sbjct: 201 YIAEIAPENMR 233
>BQ157994
Length = 960
Score = 28.1 bits (61), Expect = 8.6
Identities = 12/28 (42%), Positives = 16/28 (56%)
Frame = -1
Query: 494 CKGFSIICASVCMVSYLFLSPIHHLVVF 521
C GF+IIC V + S LF S + + F
Sbjct: 306 CDGFNIICVFVSLFSLLFFSVVRFEIPF 223
>AW691772
Length = 505
Score = 28.1 bits (61), Expect = 8.6
Identities = 15/34 (44%), Positives = 18/34 (52%)
Frame = +3
Query: 492 FECKGFSIICASVCMVSYLFLSPIHHLVVFFFFM 525
F C F +C V SY +S +HH VFFF M
Sbjct: 159 FSCFCFMSMC*LVFFQSYNIIS-LHHTYVFFFLM 257
>TC85673 similar to SP|O81361|RS8_PRUAR 40S ribosomal protein S8. [Apricot]
{Prunus armeniaca}, partial (97%)
Length = 966
Score = 28.1 bits (61), Expect = 8.6
Identities = 10/26 (38%), Positives = 18/26 (68%)
Frame = +3
Query: 395 IQIYMFIWLAENFITYISIMKVIEFN 420
IQ+Y+F WL+ N+ S ++++ FN
Sbjct: 804 IQVYVFFWLSVNWTPGTSFIQILLFN 881
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.339 0.147 0.482
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,229,430
Number of Sequences: 36976
Number of extensions: 334463
Number of successful extensions: 3063
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 3018
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3062
length of query: 526
length of database: 9,014,727
effective HSP length: 101
effective length of query: 425
effective length of database: 5,280,151
effective search space: 2244064175
effective search space used: 2244064175
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.8 bits)
S2: 61 (28.1 bits)
Medicago: description of AC140027.12