Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC136450.2 + phase: 0 /pseudo
         (198 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BI262818 weakly similar to GP|6642775|gb| gag-pol polyprotein {V...    46  8e-06
TC93065                                                                44  3e-05
CB892913 similar to GP|1769897|emb lectin receptor kinase {Arabi...    32  0.12
TC85043                                                                29  1.4
TC89751 similar to PIR|D84904|D84904 hypothetical protein At2g46...    28  1.8
AW691770 similar to PIR|B86473|B8 hypothetical protein AAG27107....    28  2.3
TC88380 similar to GP|4324495|gb|AAD16897.1| glutamyl-tRNA reduc...    28  2.3
BQ150639                                                               28  3.0
TC80056 similar to GP|12322859|gb|AAG51421.1 unknown protein  co...    27  4.0
TC81377 phosphate transporter PHT2-1 [Medicago truncatula]             27  4.0
TC81992 weakly similar to PIR|T47841|T47841 hypothetical protein...    26  8.8
TC85130 similar to GP|6642643|gb|AAF20224.1| unknown protein {Ar...    26  8.8
BQ135446 similar to GP|537296|gb|AA chalcone reductase {Medicago...    26  8.8

>BI262818 weakly similar to GP|6642775|gb| gag-pol polyprotein {Vitis
           vinifera}, partial (24%)
          Length = 615

 Score = 46.2 bits (108), Expect = 8e-06
 Identities = 31/147 (21%), Positives = 72/147 (48%)
 Frame = +1

Query: 12  VPKFNGDLEEFS*WKTNFYNHVMSLDEELWDILEGGIGDLVVDEEGADVDRRKHTPAQKK 71
           +P F+G  E +  W      ++ + D  LW+ +E     L + +       + H   + +
Sbjct: 175 LPVFDG--ENYHIWAARIEAYLEAND--LWEAVEEDYEVLPLSDNPTMAQIKNHKERKTR 342

Query: 72  LYKKHHTIRGALVKAIPKAEYMKMSDKSTAKSMFASLCANYEGSKKVREAKALMLVHQYE 131
             K     R  L  A+ +  + ++    +A  ++  L   YEG +++R  +AL L+ ++E
Sbjct: 343 KSKA----RATLFAAVSEEIFTRIMTIKSAFEIWNFLKTEYEGDERIRGMQALNLIREFE 510

Query: 132 LFKMKDGESIEQMYSRFHTLVSGLQIL 158
           + KMK+ E+I++  ++  ++ + +++L
Sbjct: 511 MQKMKESETIKEYANKLISIANKVRLL 591


>TC93065 
          Length = 783

 Score = 44.3 bits (103), Expect = 3e-05
 Identities = 22/81 (27%), Positives = 46/81 (56%)
 Frame = +2

Query: 117 KVREAKALMLVHQYELFKMKDGESIEQMYSRFHTLVSGLQILKKSCVASDHVSKILRSLP 176
           K +E ++  L  ++E  KMK+ E++ +   R   +V+ +++L +       V KIL  LP
Sbjct: 188 KNKENESAKLRREFEALKMKETETVREFSDRISKVVTQIRLLGEDLSDQRVVEKILVCLP 367

Query: 177 ARWRPKVTAIEETKDLNTLSV 197
             +  K++++EE K+ + ++V
Sbjct: 368 EMFEAKISSLEENKNFSEITV 430


>CB892913 similar to GP|1769897|emb lectin receptor kinase {Arabidopsis
           thaliana}, partial (35%)
          Length = 837

 Score = 32.3 bits (72), Expect = 0.12
 Identities = 15/31 (48%), Positives = 22/31 (70%)
 Frame = +1

Query: 168 VSKILRSLPARWRPKVTAIEETKDLNTLSVQ 198
           + KILRSL  ++   V  IEETKDL T++++
Sbjct: 559 MEKILRSLDPKFEHIVEKIEETKDLETMTIE 651


>TC85043 
          Length = 308

 Score = 28.9 bits (63), Expect = 1.4
 Identities = 17/44 (38%), Positives = 22/44 (49%)
 Frame = +3

Query: 111 NYEGSKKVREAKALMLVHQYELFKMKDGESIEQMYSRFHTLVSG 154
           NYEG KK  +   L L  QY LF+     +I Q+Y  F  +  G
Sbjct: 102 NYEGKKKNVKYSPLELERQYGLFESIVLPNIYQVYPMFFLIKKG 233


>TC89751 similar to PIR|D84904|D84904 hypothetical protein At2g46560
           [imported] - Arabidopsis thaliana, partial (11%)
          Length = 1446

 Score = 28.5 bits (62), Expect = 1.8
 Identities = 22/83 (26%), Positives = 38/83 (45%)
 Frame = +1

Query: 65  HTPAQKKLYKKHHTIRGALVKAIPKAEYMKMSDKSTAKSMFASLCANYEGSKKVREAKAL 124
           H+      Y K H + G L   IPKA    ++  +T  +    L  + +G  K+ +A++ 
Sbjct: 631 HSSLTSLNYDKDHNVDGMLWY-IPKAHSASVTKIATIPNTSLFLTGSTDGDVKLWDAESS 807

Query: 125 MLVHQYELFKMKDGESIEQMYSR 147
            L+H +   K+ D  +  Q  SR
Sbjct: 808 KLLHHWS--KLHDKHTFLQSGSR 870


>AW691770 similar to PIR|B86473|B8 hypothetical protein AAG27107.1 [imported]
           - Arabidopsis thaliana, partial (11%)
          Length = 635

 Score = 28.1 bits (61), Expect = 2.3
 Identities = 18/46 (39%), Positives = 23/46 (49%)
 Frame = +2

Query: 14  KFNGDLEEFS*WKTNFYNHVMSLDEELWDILEGGIGDLVVDEEGAD 59
           KF G+L+EF+       NH  SL   L  +  GG+   V  EEG D
Sbjct: 386 KFEGELQEFA-------NHAFSLRCVLECLQSGGVASDVKVEEGFD 502


>TC88380 similar to GP|4324495|gb|AAD16897.1| glutamyl-tRNA reductase
            precursor {Glycine max}, partial (92%)
          Length = 1689

 Score = 28.1 bits (61), Expect = 2.3
 Identities = 15/32 (46%), Positives = 20/32 (61%)
 Frame = -3

Query: 146  SRFHTLVSGLQILKKSCVASDHVSKILRSLPA 177
            S+  T VS L IL K+C+ S    K+L SLP+
Sbjct: 1666 SKTETSVSRLNILFKACIFSSVSLKVLLSLPS 1571


>BQ150639 
          Length = 257

 Score = 27.7 bits (60), Expect = 3.0
 Identities = 12/34 (35%), Positives = 17/34 (49%)
 Frame = +2

Query: 148 FHTLVSGLQILKKSCVASDHVSKILRSLPARWRP 181
           FH L   L +LK+ C ++D   +   S    WRP
Sbjct: 29  FHVLEQELHLLKEPCDSNDQRGEKTPSYQGNWRP 130


>TC80056 similar to GP|12322859|gb|AAG51421.1 unknown protein  contains
           TNFR/NGFR cysteine-rich region; 28705-27186 {Arabidopsis
           thaliana}, partial (98%)
          Length = 879

 Score = 27.3 bits (59), Expect = 4.0
 Identities = 16/51 (31%), Positives = 28/51 (54%)
 Frame = -2

Query: 146 SRFHTLVSGLQILKKSCVASDHVSKILRSLPARWRPKVTAIEETKDLNTLS 196
           SRF    SG   L K+ +     SKILR++ + + P+++   + K+ N+ S
Sbjct: 539 SRFWNFASGTFFL*KNDIIIGISSKILRNILSLYCPRISKAGQKKEQNSNS 387


>TC81377 phosphate transporter PHT2-1 [Medicago truncatula]
          Length = 2236

 Score = 27.3 bits (59), Expect = 4.0
 Identities = 10/16 (62%), Positives = 14/16 (87%)
 Frame = -1

Query: 147  RFHTLVSGLQILKKSC 162
            RFH L+ GL++L+KSC
Sbjct: 1195 RFHKLMPGLELLEKSC 1148


>TC81992 weakly similar to PIR|T47841|T47841 hypothetical protein T2O9.150 -
           Arabidopsis thaliana, partial (3%)
          Length = 952

 Score = 26.2 bits (56), Expect = 8.8
 Identities = 19/80 (23%), Positives = 40/80 (49%)
 Frame = +3

Query: 93  MKMSDKSTAKSMFASLCANYEGSKKVREAKALMLVHQYELFKMKDGESIEQMYSRFHTLV 152
           +K   K+  +    SL   Y+ + + + A+   L   + + +MK+GESI   ++R  T+V
Sbjct: 660 LKQF*KTRHRRRSGSLKK*YQVTARGKRAQLQAL*RDW*ILQMKNGESIIDYFARTVTIV 839

Query: 153 SGLQILKKSCVASDHVSKIL 172
           + +++  +       + KIL
Sbjct: 840 NKIRMHGEQMSNLTVIEKIL 899


>TC85130 similar to GP|6642643|gb|AAF20224.1| unknown protein {Arabidopsis
           thaliana}, partial (37%)
          Length = 775

 Score = 26.2 bits (56), Expect = 8.8
 Identities = 9/22 (40%), Positives = 15/22 (67%)
 Frame = -2

Query: 155 LQILKKSCVASDHVSKILRSLP 176
           L I +  C+ S+H+ K+ +SLP
Sbjct: 582 LPIAQNGCIFSEHLQKVTKSLP 517


>BQ135446 similar to GP|537296|gb|AA chalcone reductase {Medicago sativa
           subsp. sativa}, partial (14%)
          Length = 718

 Score = 26.2 bits (56), Expect = 8.8
 Identities = 13/53 (24%), Positives = 31/53 (57%)
 Frame = -1

Query: 130 YELFKMKDGESIEQMYSRFHTLVSGLQILKKSCVASDHVSKILRSLPARWRPK 182
           + +F++K G+ +   YS++       ++L++ C+A+++   ++ S P   RPK
Sbjct: 454 FPVFRVKVGDVLRIRYSQYSPY*---RVLRQFCIAANYKMSMIFSNPKFSRPK 305


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.319    0.134    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,448,237
Number of Sequences: 36976
Number of extensions: 63151
Number of successful extensions: 263
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 263
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 263
length of query: 198
length of database: 9,014,727
effective HSP length: 91
effective length of query: 107
effective length of database: 5,649,911
effective search space: 604540477
effective search space used: 604540477
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)


Medicago: description of AC136450.2